研究者業績
  1. 研究者検索結果一覧
  2. 奥田 浩

奥田 浩

オクダ ヒロシ  (Hiroshi Okuda)

基本情報

所属
自治医科大学 医学部数学 教授
J-GLOBAL ID
201401009209723250
researchmap会員ID
B000238167
外部リンク

研究キーワード

 18

研究分野

 5

経歴

 15
もっとみる

受賞

 1

論文

 55
  • S Iwamoto, JP Li, N Sugimoto, H Okuda, E Kajii
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 222(3) 852-859 1996年5月  査読有り責任著者
    We have previously identified a novel first exon of Duffy gene and two inverse GATA motifs in the 600 bp 5' flanking region. The proximal GATA is positioned downstream from the start position of endothelium and upstream from that of erythroid. One base substitution (-365T-->C) was found in the proximal GATA motif from three black Fy(a-b-) individuals, and was regarded as a common polymorphic mutation in black Fy(a-b-) individuals. The upstream sequence of the novel first exon was inserted in the upstream of chloramphenicol acetyltransferase (CAT) gene and transfected in human erythroleukemia cell line (HEL) and human microvascular endothelial cells (HMvEC). The black type mutation abolished the CAT transcription in HEL cells but not in HMvEC. Deletion mutagenesis study revealed that the proximal GATA motif represent the erythroid regulatory core region for Duffy gene. Gel shift assay showed that the proximal GATA motif is the target sequence of GATA-1. These studies indicate that the black type mutation abolishes Duffy gene expression in erythroid but not in postcapillary venule endothelium, which is compatible with the Northern blot and immunohistochemical observation in black Fy(a-b-) individuals. (C) 1996 Academic Press, Inc.
  • 小谷和彦, 奥田浩, 小山田隆, 梶井英治, 池本卯典
    自治医大紀要 18 139-146 1995年  査読有り責任著者
  • 植木寿一, 秋藤洋一, 田中孝幸, 奥田浩, 松ノ谷真智子, 松本尚美, 石河健, 中本周他
    鳥取医学雑誌 21 290-293 1993年  査読有り責任著者
  • 田中孝幸, 植木寿一, 安東吾郎, 大谷恭一, 奥田浩, 加藤一吉 他
    鳥取医学雑誌 16 112-117 1988年  査読有り責任著者
  • 奥田浩, 山本隆, 新鞍誠, 梶井英治, 土田修一, 池本卯典
    自治医大紀要 9 163-172 1986年  査読有り筆頭著者

MISC

 3
  • M Kumada, S Iwamoto, T Kamesaki, H Okuda, E Kajii
    GENE 299(1-2) 165-172 2002年10月  
    The mouse genomic sequence of the region containing the gene Rhced, the orthologue to the human gene RH30, was determined to elucidate the structure of Rhced and its flanking regions and to compare these with the corresponding human genomic region. Two genes, Smpl and AK003528 (an orthologue of FLJ10747), flank Rhced. Neither sequences homologous to the characteristic nucleotide elements flanking the RHD gene in humans (rhesus boxes) nor an additional Rh gene were found within the mouse region sequenced. This result and that of a previous report demonstrate that this chromosomal region of the mouse comprises five genes (FLJI0747-RHCE-SMP1-NPD014-P29) that exhibit syntenic homology with the corresponding human region, which suggests that the RHD gene and rhesus boxes were inserted later. Evaluations of tissue distribution and subcellular localization of these genes indicate that the SMPI orthologue has a ubiquitous tissue distribution and cytoplasmic localization, whereas AK003528 is expressed slightly higher in testis with a strong subcellular localization in the nucleus. Despite the steady improvements in the draft sequence of the human genome, this study demonstrates the continuing benefits of comparative genetic analyses in increasing our understanding of human genomic structure. (C) 2002 Elsevier Science B.V. All rights reserved.
  • 岩本 禎彦, 亀崎 豊実, 熊田 真樹, 奥田 浩, 袴田 陽二, 小林 英司, 梶井 英治
    日本輸血学会雑誌 47(2) 200-200 2001年4月  
  • 亀崎豊実, 岩本禎彦, 近江俊徳, 奥田浩, 田中光信, 高橋順子, 瀬尾たい子, 谷慶彦, 梶井英治
    日本法医学雑誌 54(2) 2000年  

書籍等出版物

 7
もっとみる

講演・口頭発表等

 84
もっとみる

担当経験のある科目(授業)

 8
もっとみる

共同研究・競争的資金等の研究課題

 11
もっとみる
一覧へ戻る