医学部 生理学講座

尾仲 達史

Tatsushi Onaka

基本情報

所属
自治医科大学 医学部生理学講座 神経脳生理学部門 教授
学位
医学博士

J-GLOBAL ID
200901019055785792
researchmap会員ID
1000063236

外部リンク

受賞

 2

論文

 166
  • 犬束 歩, 尾仲 達史
    糖尿病・内分泌プラクティスWeb はじめに ストレスは食欲や睡眠といった生理現象に大きな影響を与え、行動面での変化にもつながる。こうした生理応答や行動変容においては、ストレスによって脳内で分泌される神経ペプチドが重要な役割を果たしている。 2023年7月  
  • 犬束 歩, 吉田 匡秀, 高柳 友紀, 尾仲 達史
    日本内分泌学会雑誌 99(1) 177-177 2023年5月  
  • Shota Okabe, Yuki Takayanagi, Masahide Yoshida, Tatsushi Onaka
    iScience 26(3) 106243-106243 2023年3月  
  • Masahide Yoshida, Tomoko Saito, Yuki Takayanagi, Yoshikazu Totsuka, Tatsushi Onaka
    Scientific Reports 12(1) 20390-20390 2022年11月  査読有り
  • Ayumu Inutsuka, Sho Maejima, Hiroyuki Mizoguchi, Ryosuke Kaneko, Rei Nomura, Keiko Takanami, Hirotaka Sakamoto, Tatsushi Onaka
    Communications biology 5(1) 979-979 2022年9月16日  
    Abstract Transgenic animals expressing fluorescent proteins are widely used to label specific cells and proteins. GFP-dependent gene regulation utilizes these lines to manipulate gene expression; however, its application has been limited to fluorescent proteins derived from Aequorea jellyfish. By using a split Cre recombinase fused with mCherry-binding nanobodies or designed ankyrin repeat proteins, we created Cre recombinase dependent on red fluorescent protein (RFP) (Cre-DOR). Functional binding units for monomeric RFPs (mCherry, mRFP1) are different from those for dimeric RFP (tdTomato). We confirmed target RFP-dependent gene expression in the mouse cerebral cortex using stereotaxic injection of adeno-associated virus vectors including Cre-DOR, target RFP, and reporter GFP vector. We found highly selective GFP expression in RFP-positive cortical neurons with 93.5 ± 0.6% of GFP-positive cells being mRFP1-positive. In estrogen receptor-beta (Esr2)-mRFP1 mice, we confirmed that Cre-DOR can be used for selective expression of membrane-bound GFP in the paraventricular nucleus of the hypothalamus. The neural projection from Esr2-expressing neurons in the hypothalamic paraventricular nucleus to the posterior pituitary was visualized by Cre-DOR. In gastrin-releasing peptide receptor (Grpr)-mRFP1 rats, we similarly achieved anterograde tracing of Grpr-expressing neurons in the medial amygdala and found that they are projecting axons to the posterior bed nucleus of the stria terminalis. Cellular localization of RFPs affects recombination efficiency of Cre-DOR, and light and chemical-induced nuclear translocation of an RFP-fused protein can increase or decrease Cre-DOR efficiency. Our results provide a method for manipulating gene expression in specific cells expressing RFPs and expand the repertory of nanobody-based genetic tools.

MISC

 165

共同研究・競争的資金等の研究課題

 38