研究者業績

早川 枝李

ハヤカワ エリ  (Eri Saki H. Hayakawa)

基本情報

所属
自治医科大学 医学部感染・免疫学講座 医動物学部門
学位
博士(工学)(北海道大学)
工学修士(北海道大学)

J-GLOBAL ID
201401099853595629
researchmap会員ID
B000238861

委員歴

 2

主要な論文

 27
  • Eri Saki, H. Hayakawa, Marina Wayama, Fuyuki Tokumasu, Nobuhiko Ohno, Mami Matsumoto, Jiro Usukura
    Frontiers in Cellular and Infection Microbiology 12 962495-962495 2022年8月22日  査読有り筆頭著者責任著者
  • Fuyuki Tokumasu, Eri H. Hayakawa, Junpei Fukumoto, Suzumi M. Tokuoka, Shinya Miyazaki
    Parasitology International 83 102369 2021年8月  査読有り招待有り
  • Eri H. Hayakawa, Hirotomo Kato, Glenn A. Nardone, Jiro Usukura
    Parasitology International 80 102179-102179 2021年2月  査読有り筆頭著者責任著者
  • Eri H. Hayakawa,, Kentaro Yamaguchi, Masahiro Mori, Glenn Nardone
    Scientific Report 10 1-12 2020年2月17日  査読有り筆頭著者責任著者
  • Eri H. Hayakawa, Fuyuki Tokumasu, Jiro Usukura, Hiroyuki Matsuoka, Takafumi Tsuboi, Thomas E.Wellems
    Experimental parasitology 153 174-179 2015年6月  査読有り筆頭著者責任著者
  • Eri H. Hayakawa, Eiko Mochizuki, Tetsuya Tsuda, Kazunari Akiyoshi, Hiroyuki Matsuoka, Susumu Kuwabata
    PLoS ONE 8(12) e85467 2013年12月31日  査読有り筆頭著者責任著者
    Ionic liquids (ILs) are room-temperature molten salts that have applications in both physical sciences and more recently in the purification of proteins and lipids, gene transfection and sample preparation for electron microscopy (EM) studies. Transfection of genes into cells requires membrane fusion between the cell membrane and the transfection reagent, thus, ILs may be induce a membrane fusion event. To clarify the behavior of ILs with cell membranes the effect of ILs on model membranes, i.e., liposomes, were investigated. We used two standard ILs, 1-ethyl-3-methylimidazolium lactate ([EMI][Lac]) and choline lactate ([Ch][Lac]), and focused on whether these ILs can induce lipid vesicle fusion. Fluorescence resonance energy transfer and dynamic light scattering were employed to determine whether the ILs induced vesicle fusion. Vesicle solutions at low IL concentrations showed negligible fusion when compared with the controls in the absence of ILs. At concentrations of 30% (v/v), both types of ILs induced vesicle fusion up to 1.3 and 1.6 times the fluorescence intensity of the control in the presence of [Ch][Lac] and [EMI] [Lac], respectively. This is the first demonstration that [EMI][Lac] and [Ch][Lac] induce vesicle fusion at high IL concentrations and this observation should have a significant influence on basic biophysical studies. Conversely, the ability to avoid vesicle fusion at low IL concentrations is clearly advantageous for EM studies of lipid samples and cells. This new information describing IL-lipid membrane interactions should impact EM observations examining cell morphology. © 2013 Hayakawa et al.
  • Eri H. Hayakawa, Michiko Furutani, Rumiko Matsuoka, Yuichi Takakuwa
    J Physiol Sci 15 2011年5月  査読有り筆頭著者責任著者
  • Eri Hayakawa, Fuyuki Tokumasu, Glenn A. Nardone, Albert J. Jin, Vince A. Hackley, James A. Dvorak
    BIOPHYSICAL JOURNAL 93(11) 4018-4030 2007年12月  査読有り筆頭著者
    Tuberculosis is an infectious and potentially fatal disease caused by the acid-fast bacillus Mycobacterium tuberculosis (MTB). One hallmark of a tuberculosis infection is the ability of the bacterium to subvert the normal macrophage defense mechanism of the host immune response. Lipoarabinomannan ( LAM), an integral component of the MTB cell wall, is released when MTBs are taken into phagosomes and has been reported to be involved in the inhibition of phago-lysosomal (P-L) fusion. However, the physical chemistry of the effects of LAM on lipid membrane structure relative to P-L fusion has not been studied. We produced membranes in vitro composed of dioleoylphosphatidylcholine, sphingomyelin, and cholesterol to simulate phagosomal lipid membranes and quantified the effects of the addition of LAM to these membranes, using fluorescence resonance energy transfer assays and atomic force microscopy. We found that LAM inhibits vesicle fusion and markedly alters lipid membrane domain morphology and sphingomyelin-chollesterol/dioleoylphosphatidylcholine ratios. These data demonstrate that LAM induces a dramatic reorganization of lipid membranes in vitro and clarifies the role of LAM in the inhibition of P-L fusion and the survival of the MTB within the macrophage.

MISC

 9

書籍等出版物

 2
  • Eri H. Hayakawa, Yuichi Takakuwa (担当:分担執筆, 範囲:Chapter 33: The Study of a Protein Molecular Motion by Fluorescence Correlation Spectroscopy (FCS))
    2007年  Refereed
  • (担当:共著, 範囲:マラリアなど原虫の検査)
    2005年  Refereed

講演・口頭発表等

 39

共同研究・競争的資金等の研究課題

 4

その他

 1
  • 2023年1月 - 現在
    2022年に公開された論文; “Budding pouches and associated bubbles: 3D visualization of exo-membrane structures in plasmodium falciparum gametocytes”が、Medicine Innovates Series (https://medicineinnovates.com/)に ブレイクスルーとして採択された。 https://medicineinnovates.com/budding-pouches-associated-bubbles-3d-visualization-exo-membrane-structures-plasmodium-falciparum-gametocytes/