早川 枝李

Although the neuronal protein α-synuclein (α-syn) is thought to play a central role in the pathogenesis of Parkinson's disease (PD), its physiological function remains unknown. It is known that α-syn is also abundantly expressed in erythrocytes. However, its role in erythrocytes is also unknown. In the present study, we investigated the localization of α-syn in human erythroblasts and erythrocytes. Protein expression of α-syn increased during terminal differentiation of erythroblasts (from day 7 to day 13), whereas its mRNA level peaked at day 11. α-syn was detected in the nucleus, and was also seen in the cytoplasm and at the plasma membrane after day 11. In erythroblasts undergoing nucleus extrusion (day 13), α-syn was detected at the periphery of the nucleus. Interestingly, we found that recombinant α-syn binds to trypsinized inside-out vesicles of erythrocytes and phosphatidylserine (PS) liposomes. The dissociation constants for binding to PS/phosphatidylcholine (PC) liposomes of N-terminally acetylated (NAc) α-syn was lower than that of non NAc α-syn. This suggests that N-terminal acetylation plays a significant functional role. The results of the present study collectively suggest that α-syn is involved in the enucleation of erythroblasts and the stabilization of erythroid membranes.

2011年1月から2014年12月までに医動物学部門へ照会された120症例について報告する。マラリア根治療法前(46例)とマラリア以外の疾患治療前や溶血性疾患除外目的(14例)にglucose-6-phosphate-dehydrogenase(G6PD)欠損症スクリーニング依頼があり、新生児黄疸を示したコートジボワール出身の男児と、フィリピン出身の母親と息子2人に欠損症を認めた。腸管内寄生虫症として日本海裂頭条虫症8例、アジア条虫症2例、アニサキス症6例、蟯虫症2例、鉤虫・糞線虫・回虫症各1例、ヒトブラストシスチス1例が照会され、腸管外寄生虫症としてトキソプラズマ症3例、肺吸虫・肝蛭・マンソン孤虫症各1例が照会された。衛生動物関連疾患9例の内マダニ咬症が6例(発熱1例)を占めた。発熱症例のマラリア除外依頼6例、その他の照会が17例(旅行者下痢症、渡航前相談、寄生虫疾患の除外)であった。当