基本情報
- 所属
- 自治医科大学 医学部生理学講座生物物理学部門 教授
- 学位
- 工学博士(大阪大学)
- 研究者番号
- 20196439
- J-GLOBAL ID
- 200901080671475544
- researchmap会員ID
- 1000063201
- 外部リンク
研究キーワード
8経歴
1学歴
4-
- 1985年
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- 1985年
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- 1983年
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- 1983年
委員歴
1-
2010年1月 - 現在
論文
88-
Journal of synchrotron radiation 30(Pt 2) 368-378 2023年3月1日X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter-scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH.
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The Journal of Physical Chemistry B 126(51) 10797-10812 2022年12月29日
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Biochemical and Biophysical Research Communications 635 277-282 2022年12月
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Biophys. J. 121(14) 2767-2780 2022年6月 査読有り
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PLOS ONE 17(5) e0261699-e0261699 2022年5月5日We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 kinase inhibitors, and succeeded in screening two inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.
MISC
39-
Japanese Journal of Applied Physics 59(1) 2020年1月1日X-ray fluorescence holography (XFH) is a technique that can directly image the 3D arrangement of atoms around an element in a sample. The holograms contain both intensity and phase information, allowing atomic reconstruction without needing prior structural information or a tentative structural model. XFH has already been used to reveal the local structures of various inorganic samples, and recently, work has begun on XFH for soft matter. In this paper, we review the progress of XFH on soft materials. First, we review the fundamental principles of XFH. Second, we review inverse mode XFH on soft materials, and the results of the experiments on hemoglobin, myoglobin, and κ-(BEDT-TTF)2Cu[N(CN)2]Br crystals. In the last section, we report the progress of the development of normal mode holography for soft materials. The new apparatus and scanning method is described, and results of the initial tests on the protein Photosystem II are discussed.
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日本農芸化学会大会講演要旨集(Web) 2016 2C004 (WEB ONLY) 2016年3月5日
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1st Asia-Oceania Conference on Neutron Scattering 2011年11月23日
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ACA 2008 (Meeting of the American Crystallographic Association) 179 2008年
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IUCr2008 Satellite symposium 2008年
講演・口頭発表等
43担当経験のある科目(授業)
11共同研究・競争的資金等の研究課題
17-
日本学術振興会 科学研究費助成事業 2020年4月 - 2023年3月
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日本学術振興会 科学研究費助成事業 2019年4月 - 2023年3月
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文部科学省 科学研究費補助金(基盤研究(C)) 2016年4月 - 2019年3月
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文部科学省 科学研究費補助金(新学術領域研究) 2014年7月 - 2019年3月
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文部科学省 科学研究費補助金(新学術領域研究) 2015年4月 - 2017年3月