Artoni Kevin R Ang Yasufumi Umena Ayana Sato-Tomita Naoya Shibayama Naohisa Happo Riho Marumi Yuta Yamamoto Koji Kimura Naomi Kawamura Yu Takano Tomohiro Matsushita Yuji C Sasaki Jian Ren Shen Kouichi Hayashi
Journal of synchrotron radiation 30(Pt 2) 368-378 2023年3月
X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the ...
We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3...
The Journal of Physical Chemistry Letters 12(xxxx) 2172-2176 2021年2月 [査読有り]
Hydration water plays a crucial role for activating the protein dynamics required for functional expression. Yet, the details are not understood about how hydration water couples with protein dynamics. A temperature hysteresis of the ice formation...
Naoya Shibayama Ayana Sato-Tomita Mio Ohki Kouhei Ichiyanagi Sam-Yong Park
Proceedings of the National Academy of Sciences 117(9) 4741-4748 2020年3月 [査読有り]
Hemoglobin is one of the best-characterized proteins with respect to structure and function, but the internal ligand diffusion pathways remain obscure and controversial. Here we captured the CO migration processes in the tense (T), relaxed (R), an...
Artoni Kevin R. Ang Ayana Sato-Tomita Naoya Shibayama Yasufumi Umena Naohisa Happo Riho Marumi Koji Kimura Tomohiro Matsushita Kazuto Akagi Takahiko Sasaki Yuji C. Sasaki Kouichi Hayashi
Japanese Journal of Applied Physics 59(1) 2020年1月
X-ray fluorescence holography (XFH) is a technique that can directly image the 3D arrangement of atoms around an element in a sample. The holograms contain both intensity and phase information, allowing atomic reconstruction without needing prior ...
A number of ligand binding studies of human adult hemoglobin (HbA) cross-linked between Lys 82beta(1) and Lys 82beta(2) with bis(3,5-dibromosalicyl)fumarate have been reported. The oxygen binding properties of native HbA, including the cooperativi...
Theory and simulations predict that the folding kinetics of protein-like heteropolymers become nonexponential and glassy (i.e., controlled by escape from different low-energy misfolded states) at low temperatures, but there was little experimental...
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 100(12) 7039-7044 2003年6月
Human Hb, an alpha(2)beta(2) tetrameric oxygen transport protein that switches from a T (tense) to an R (relaxed) quaternary structure during oxygenation, has long served as a model for studying protein allostery, in general. Time-resolved spectro...
自治医科大学
医学部