研究者氏名	谷原史倫

URL
所属	自治医科大学
部署	先端医療技術開発センター動物資源ラボラトリー
職名	准教授
学位	博士(獣医学)(山口大学)
科研費研究者番号	90754680
J-Global ID	201801008049373758

プロフィール

動物の発生工学、生殖工学分野で、ブタの体外胚生産技術やゲノム編集に関する研究を行っています。

研究キーワード

卵母細胞 ,ゲノム編集 ,体外受精 ,ブタ

研究分野

ライフサイエンス / 動物生命科学 /

経歴

2020年10月

現在

自治医科大学先端医療技術開発センター准教授

論文

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GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system.

Fuminori Tanihara Maki Hirata Zhao Namula Manita Wittayarat Lanh Thi Kim Do Qingyi Lin Koki Takebayashi Hiromasa Hara Megumi Nagahara Takeshige Otoi

Molecular biology reports 50(6) 5049-5057 2023年6月 [査読有り]

BACKGROUND: Pigs are excellent large animal models with several similarities to humans. They provide valuable insights into biomedical research that are otherwise difficult to obtain from rodent models. However, even if miniature pig strains are u...

DEVELOPMENT AND GENOME MUTATION OF BOVINE ZYGOTES VITRIFIED BEFORE AND AFTER GENOME EDITING VIA ELECTROPORATION

Thanh Van Nguyen Lanh Thi Kim Do Zhao Namula Qingyi Lin Nanaka Torigoe Megumi Nagahara Maki Hirata Fuminori Tanihara Takeshige Otoi

Cryo-Letters 44(2) 118-122 2023年3月 [査読有り]

BACKGROUND: Cryopreservation of bovine zygotes allows for a flexible schedule of genome editing via electroporation. However, vitrification-induced cell membrane damage may not only affect embryonic development but also genome mutation. OBJECTIVE:...

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN.

Fuminori Tanihara Maki Hirata Zhao Namula Lanh Thi Kim Do Naoaki Yoshimura Qingyi Lin Koki Takebayashi Tetsushi Sakuma Takashi Yamamoto Takeshige Otoi

Frontiers in cell and developmental biology 11 884340 2023年 [査読有り]

Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secre...

GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines.

Fuminori Tanihara Maki Hirata Takeshige Otoi

Methods in molecular biology (Clifton, N.J.) 2637 293-300 2023年 [招待有り]

Pigs are excellent large animal models owing to their several physiological and anatomical similarities to humans. Somatic cell nuclear transfer using gene-modified cells is the mainstream approach for generating genetically modified pigs. Recent ...

Efficiency of genetic modification in gene-knockout sperm-derived zygotes followed by electroporation of guide RNA targeting the same gene.

Koki Takebayashi Manita Wittayarat Qingyi Lin Nanaka Torigoe Bin Liu Maki Hirata Megumi Nagahara Fuminori Tanihara Takeshige Otoi

Animal science journal = Nihon chikusan Gakkaiho 94(1) e13842 2023年 [査読有り]

Genetic mosaicism is considered one of the main limitations of the electroporation method used to transfer CRISPR-Cas9/guide RNA (gRNA) into porcine zygotes. We hypothesized that fertilization of oocytes with sperm from gene-deficient boars, in co...