研究者業績

長内 康幸

オサナイ ヤスユキ  (Yasuyuki Osanai)

基本情報

所属
自治医科大学 医学部 解剖学講座 組織学部門 講師
学位
博士(理学)(総合研究大学院大学)

研究者番号
90758004
J-GLOBAL ID
202001007587820189
researchmap会員ID
R000006728

論文

 18
  • Yasuyuki Osanai, Yao Lulu Xing, Shinya Mochizuki, Kenta Kobayashi, Jihane Homman-Ludiye, Amali Cooray, Jasmine Poh, Ayumu Inutsuka, Nobuhiko Ohno, Tobias D. Merson
    Molecular Therapy - Methods and Clinical Development 32(3) 101288-101288 2024年9月  査読有り
  • バッツルガ・バトブレブ, 長内 康幸, 山崎 礼二, 矢田部 恵, 篠原 良章, 大野 伸彦
    日本組織細胞化学会総会・学術集会講演プログラム・予稿集 64回 38-38 2023年10月  
  • Yao Lulu Xing, Jasmine Poh, Bernard H A Chuang, Kaveh Moradi, Stanislaw Mitew, William D Richardson, Trevor J Kilpatrick, Yasuyuki Osanai, Tobias D Merson
    Cell reports methods 3(2) 100414-100414 2023年2月27日  
    Approaches to investigate adult oligodendrocyte progenitor cells (OPCs) by targeted cell ablation in the rodent CNS have limitations in the extent and duration of OPC depletion. We have developed a pharmacogenetic approach for conditional OPC ablation, eliminating >98% of OPCs throughout the brain. By combining recombinase-based transgenic and viral strategies for targeting OPCs and ventricular-subventricular zone (V-SVZ)-derived neural precursor cells (NPCs), we found that new PDGFRA-expressing cells born in the V-SVZ repopulated the OPC-deficient brain starting 12 days after OPC ablation. Our data reveal that OPC depletion induces V-SVZ-derived NPCs to generate vast numbers of PDGFRA+NG2+ cells with the capacity to proliferate and migrate extensively throughout the dorsal anterior forebrain. Further application of this approach to ablate OPCs will advance knowledge of the function of both OPCs and oligodendrogenic NPCs in health and disease.
  • Reiji Yamazaki, Yasuyuki Osanai, Tom Kouki, Jeffrey K Huang, Nobuhiko Ohno
    Neurochemistry international 164 105505-105505 2023年2月6日  
    Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system characterized by remyelination failure, axonal degeneration, and progressive worsening of motor functions. Animal models of demyelination are frequently used to develop and evaluate therapies for MS. We recently reported that focal internal capsule (IC) demyelination in mice with lysophosphatidylcholine injection induced acute motor deficits followed by recovery through remyelination. However, it remains unknown whether the IC demyelination mouse model can be used to evaluate changes in motor functions caused by pharmacological treatments that promote remyelination using behavioral testing and histological analysis. In this study, we examined the effect of clemastine, an anti-muscarinic drug that promotes remyelination, in the mouse IC demyelination model. Clemastine administration improved motor function and changed forepaw preference in the IC demyelinated mice. Moreover, clemastine-treated mice showed increased mature oligodendrocyte density, reduced axonal injury, an increased number of myelinated axons and thicker myelin in the IC lesions compared with control (PBS-treated) mice. These results suggest that the lysophosphatidylcholine-induced IC demyelination model is useful for evaluating changes in motor functions following pharmacological treatments that promote remyelination.
  • Batpurev Battulga, Kazuhiro Shiizaki, Yutaka Miura, Yasuyuki Osanai, Reiji Yamazaki, Yoshiaki Shinohara, Yoshiyuki Kubota, Toru Hara, Makoto Kuro-O, Nobuhiko Ohno
    Scientific reports 13(1) 852-852 2023年1月16日  
    Calcium phosphate forms particles under excessive urinary excretion of phosphate in the kidney. While the formation of calcium phosphate particles (CaPs) has been implicated in the damage to renal tubular cells and renal dysfunction, clarifying the ultrastructural information and the elemental composition of the small CaPs in the wide areas of kidney tissue has been technically difficult. This study introduces correlative and sequential light as well as electron microscopic CaP observation in the kidney tissue by combining fluorescent staining for CaPs and energy-dispersive X-ray spectroscopy (EDS) in scanning electron microscopy (SEM) on resin sections prepared using high-pressure freezing and freeze substitution. CaPs formed in mouse kidneys under long-term feeding of a high-phosphate diet were clearly visualized on resin sections by fluorescence-conjugated alendronate derivatives and toluidine blue metachromasia. These CaPs were verified by correlative observation with EDS. Furthermore, small CaPs formed in the kidney under short-term feeding were detected using fluorescent probes. The elemental composition of the particles, including calcium and magnesium, was identified following EDS analyses. These results suggest that the correlative microscopy approach is helpful for observing in situ distribution and elemental composition of CaPs in the kidney and contributing to studies regarding CaP formation-associated pathophysiology.

MISC

 16

講演・口頭発表等

 3

共同研究・競争的資金等の研究課題

 7