基本情報
- 所属
- 自治医科大学 医学部 解剖学講座 組織学部門 講師
- 学位
- 博士(理学)(総合研究大学院大学)
- 研究者番号
- 90758004
- J-GLOBAL ID
- 202001007587820189
- researchmap会員ID
- R000006728
経歴
8-
2022年11月 - 現在
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2020年4月 - 2022年10月
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2017年5月 - 2020年3月
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2015年4月 - 2017年4月
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2012年4月 - 2015年3月
受賞
4-
2024年6月
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2024年2月
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2022年9月
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2016年9月
論文
17-
Molecular Therapy - Methods and Clinical Development 32(3) 101288-101288 2024年9月 査読有り
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High-efficiency pharmacogenetic ablation of oligodendrocyte progenitor cells in the adult mouse CNS.Cell reports methods 3(2) 100414-100414 2023年2月27日Approaches to investigate adult oligodendrocyte progenitor cells (OPCs) by targeted cell ablation in the rodent CNS have limitations in the extent and duration of OPC depletion. We have developed a pharmacogenetic approach for conditional OPC ablation, eliminating >98% of OPCs throughout the brain. By combining recombinase-based transgenic and viral strategies for targeting OPCs and ventricular-subventricular zone (V-SVZ)-derived neural precursor cells (NPCs), we found that new PDGFRA-expressing cells born in the V-SVZ repopulated the OPC-deficient brain starting 12 days after OPC ablation. Our data reveal that OPC depletion induces V-SVZ-derived NPCs to generate vast numbers of PDGFRA+NG2+ cells with the capacity to proliferate and migrate extensively throughout the dorsal anterior forebrain. Further application of this approach to ablate OPCs will advance knowledge of the function of both OPCs and oligodendrogenic NPCs in health and disease.
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Neurochemistry international 164 105505-105505 2023年2月6日Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system characterized by remyelination failure, axonal degeneration, and progressive worsening of motor functions. Animal models of demyelination are frequently used to develop and evaluate therapies for MS. We recently reported that focal internal capsule (IC) demyelination in mice with lysophosphatidylcholine injection induced acute motor deficits followed by recovery through remyelination. However, it remains unknown whether the IC demyelination mouse model can be used to evaluate changes in motor functions caused by pharmacological treatments that promote remyelination using behavioral testing and histological analysis. In this study, we examined the effect of clemastine, an anti-muscarinic drug that promotes remyelination, in the mouse IC demyelination model. Clemastine administration improved motor function and changed forepaw preference in the IC demyelinated mice. Moreover, clemastine-treated mice showed increased mature oligodendrocyte density, reduced axonal injury, an increased number of myelinated axons and thicker myelin in the IC lesions compared with control (PBS-treated) mice. These results suggest that the lysophosphatidylcholine-induced IC demyelination model is useful for evaluating changes in motor functions following pharmacological treatments that promote remyelination.
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Scientific reports 13(1) 852-852 2023年1月16日Calcium phosphate forms particles under excessive urinary excretion of phosphate in the kidney. While the formation of calcium phosphate particles (CaPs) has been implicated in the damage to renal tubular cells and renal dysfunction, clarifying the ultrastructural information and the elemental composition of the small CaPs in the wide areas of kidney tissue has been technically difficult. This study introduces correlative and sequential light as well as electron microscopic CaP observation in the kidney tissue by combining fluorescent staining for CaPs and energy-dispersive X-ray spectroscopy (EDS) in scanning electron microscopy (SEM) on resin sections prepared using high-pressure freezing and freeze substitution. CaPs formed in mouse kidneys under long-term feeding of a high-phosphate diet were clearly visualized on resin sections by fluorescence-conjugated alendronate derivatives and toluidine blue metachromasia. These CaPs were verified by correlative observation with EDS. Furthermore, small CaPs formed in the kidney under short-term feeding were detected using fluorescent probes. The elemental composition of the particles, including calcium and magnesium, was identified following EDS analyses. These results suggest that the correlative microscopy approach is helpful for observing in situ distribution and elemental composition of CaPs in the kidney and contributing to studies regarding CaP formation-associated pathophysiology.
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Neurochemical research 47(9) 2815-2825 2022年8月6日An appropriate sensory experience during the early developmental period is important for brain maturation. Dark rearing during the visual critical period delays the maturation of neuronal circuits in the visual cortex. Although the formation and structural plasticity of the myelin sheaths on retinal ganglion cell axons modulate the visual function, the effects of dark rearing during the visual critical period on the structure of the retinal ganglion cell axons and their myelin sheaths are still unclear. To address this question, mice were reared in a dark box during the visual critical period and then normally reared to adulthood. We found that myelin sheaths on the retinal ganglion cell axons of dark-reared mice were thicker than those of normally reared mice in both the optic chiasm and optic nerve. Furthermore, whole-mount immunostaining with fluorescent axonal labeling and tissue clearing revealed that the myelin internodal length in dark-reared mice was shorter than that in normally reared mice in both the optic chiasm and optic nerve. These findings demonstrate that dark rearing during the visual critical period affects the morphology of myelin sheaths, shortens and thickens myelin sheaths in the visual pathway, despite the mice being reared in normal light/dark conditions after the dark rearing.
MISC
14講演・口頭発表等
3-
2019 Meeting of the Austrasian Neuroscience Society, 39th Annual Scientific Meeting. 2019年12月2日 招待有り
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ARMI seminar, Monash University 2016年12月20日 招待有り
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The 8th Nagoya global retreat. (February 13th 2016, Higashiura, Japan) 2016年2月13日 招待有り
共同研究・競争的資金等の研究課題
7-
日本学術振興会 科学研究費助成事業 2024年4月 - 2027年3月
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上原記念生命科学財団 2022年度研究奨励金 2023年3月 - 2024年3月
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日本学術振興会 科学研究費助成事業 若手研究 2021年4月 - 2024年3月
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日本学術振興会 科学研究費助成事業 研究活動スタート支援 2020年9月 - 2022年3月
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自治医科大学 自治医科大学医学部研究奨励金 2021年4月 - 2022年3月
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川野小児医学奨学財団 第33回研究助成(若手枠) 2022年度
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内藤記念科学記念財団 2016年度 内藤記念海外研究留学助成