研究者業績

佐瀬 美和子

Sase Miwako

基本情報

所属
自治医科大学 附属さいたま医療センター外科系診療部歯科口腔外科

J-GLOBAL ID
201401011277076187
researchmap会員ID
B000238699

論文

 16
  • Taku Sato, Miwako Sase, Shun Ishikawa, Mihoko Kajita, Jumpei Asano, Toshiro Sato, Yoshiyuki Mori, Toshiaki Ohteki
    Scientific reports 10(1) 8308-8308 2020年5月22日  
    The small intestine has a robust regenerative capacity, and various cell types serve as "cells-of-origin" in the epithelial regeneration process after injury. However, how much each population contributes to regeneration remains unclear. Using lineage tracing, we found that Lgr5-expressing cell derivatives contained radioresistant intestinal stem cells (ISCs) crucial for epithelial regeneration in the damaged intestine after irradiation. Single-cell qRT-PCR analysis showed that surviving Lgr5-expressing cell derivatives in the damaged intestine are remarkably heterogeneous, and that the expression levels of a YAP-target gene Sca1 were inversely correlated with their "stemness", suggesting that the YAP/Wnt signal balance in surviving crypt epithelial cells determines the cellular contribution to epithelial regeneration. Single-cell RNA sequencing of Sca1-Lgr5-derivatives revealed that expression of a tetraspanin family member CD81 correlated well with the expression of ISC- and proliferation-related genes. Consistent with these findings, organoid-forming ability was confined to the CD81hiSca1- fraction within the damaged crypt epithelial cells. Characterization of radioresistant epithelial stem cell heterogeneity in the damaged intestine may contribute to therapeutic strategies for gastrointestinal diseases.
  • 佐瀬美和子, 佐瀬美和子, 浅井昌大, 神部芳則, 森良之
    日本口腔外科学会雑誌 64(6) 361‐366 2018年6月20日  
  • Sase Miwako, Itoh Hiroto, Nakano Takahumi, Hayasaka Junichi, Noguchi Tadahide, Jinbu Yoshinori, Mori Yoshiyuki
    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 29(5) 438-442 2017年9月1日  査読有り
  • Takafumi Nakano, Yoshinori Jinbu, Naruo Okada, Miwako Sase, Junichi Hayasaka, Yoshiyuki Tsuchiya, Hiroto Itoh, Tadahide Noguchi, Kentaro Hoshi, Yoshiyuki Mori
    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 28(6) 525-529 2016年11月1日  査読有り
  • Miwako Sase, Hiroto Itoh, Junichi Hayasaka, Kentaro Hoshi, Tadahide Noguchi, Yoshinori Jinbu, Yoshiyuki Mori
    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 28(6) 493-497 2016年11月1日  査読有り

MISC

 6

共同研究・競争的資金等の研究課題

 2