池上 昭彦

Upstream element of Pressure-regulated operon from a deep-sea barotolerant bacterium strain DSS12 under several pressures was studied. Region A and region B were classified by sequence analysis. A unique octamer motif AAGGTAAG, was found to be tandemly repeated 13 times in Region B. Electrophoretic mobility shift assay demonstrated that σ⁵⁴-like factor recognized Region A and another factors recognized Region B of the operon. Different shift patterns of the protein-DNA complexes were observed when cell extracts cultured at 0. 1MPa and 50MPa were incubated with the DNA probe of Region B. These results indicate that the strain expresses different DNA-binding factors under different pressure conditions.