医学部 解剖学講座

望月 信弥

Shinya Mochizuki

基本情報

所属
自治医科大学 医学部 解剖学講座 解剖学部門 助教
学位
医学博士(自治医科大学)

J-GLOBAL ID
201901010260875346
researchmap会員ID
B000358673

論文

 3
  • Yasuyuki Osanai, Yao Lulu Xing, Shinya Mochizuki, Kenta Kobayashi, Jihane Homman-Ludiye, Amali Cooray, Jasmine Poh, Ayumu Inutsuka, Nobuhiko Ohno, Tobias D. Merson
    Molecular Therapy - Methods & Clinical Development 32(3) 101288-101288 2024年9月  査読有り
  • Shinya Mochizuki, Harukata Miki, Ruyun Zhou, Yasuko Noda
    Biochemistry and Biophysics Reports 30 101257-101257 2022年7月  査読有り筆頭著者
    Oxysterol-binding protein (OSBP)-related protein (ORP) 6, a member of subfamily III in the ORP family, localizes to membrane contact sites between the endoplasmic reticulum (ER) and other organelles and functions in non-vesicular exchange of lipids including phosphatidylinositol-4-phosphate (PI4P) in neurons. In this study, we searched for the lipid counter-transported in exchange for PI4P by using molecular cell biology techniques. Deconvolution microscopy revealed that knockdown of ORP6 partially shifted localization of a phosphatidylserine (PS) marker but not filipin in primary cultured cerebellar neurons. Overexpression of ORP6 constructs lacking the OSBP-related ligand binding domain (ORD) resulted in the same shift of the PS marker. A PI4KⅢα inhibitor specifically inhibiting the synthesis and plasma membrane (PM) localization of PI4P, suppressed the localization of ORP6 and the PS marker at the PM. Overexpression of mutant PS synthase 1 (PSS1) inhibited transport of the PS marker to the PM and relocated the PI4P marker to the PM in Neuro-2A cells. Introduction of ORP6 but not the dominant negative ORP6 constructs, shifted the localization of PS back to the PM. These data collectively suggest the involvement of ORP6 in the counter-transport of PI4P and PS.
  • Shinya Mochizuki, Harukata Miki, Ruyun Zhou, Yukiharu Kido, Wataru Nishimura, Motoshi Kikuchi, Yasuko Noda
    Experimental Cell Research 370(2) 601-612 2018年9月  査読有り筆頭著者

MISC

 14

講演・口頭発表等

 19

担当経験のある科目(授業)

 5

共同研究・競争的資金等の研究課題

 4

社会貢献活動

 5