研究者総覧

牧野 伸子 (マキノ ノブコ)

  • 公衆衛生学部門 教授
Last Updated :2021/10/17

研究者情報

学位

  • 医学博士(自治医科大学)

ホームページURL

J-Global ID

研究キーワード

  • 公衆衛生学   疫学   川崎病   難治性疾患   嗅覚   細胞・組織   好酸球   

研究活動情報

論文

  • 自治医科大学女性卒業生を対象とした就業継続に関する調査結果
    牧野伸子, 仲摩恵美, 吉村成子, 竹内三春, 雲井悠里, 河原奈麻美, 石川由紀子, 牧野伸二, 松村正巳
    月刊地域医学 30 6 462 - 466 2017年06月 [査読有り][通常論文]
  • Analysis of Neuroblastoma Mortality Rates before and after the Cessation of the Mass Screening Program According to Vital Statistics of Japan Data
    Makino N, Nakamura Y, Ikeda H, Oka A
    Epidemiol: Open Access 7 2 161 - 165 2017年 [査読有り][通常論文]
  • Nobuko Makino, Yosikazu Nakamura, Mayumi Yashiro, Ryusuke Ae, Satoshi Tsuboi, Yasuko Aoyama, Takao Kojo, Ritei Uehara, Kazuhiko Kotani, Hiroshi Yanagawa
    Journal of Epidemiology 25 3 239 - 245 2015年 [査読有り][通常論文]
     
    Background: The number of patients and incidence rate of Kawasaki disease (KD) are increasing in Japan. We have therefore characterized the latest epidemiological information on KD. Methods: The 22nd nationwide survey of KD, which targeted patients diagnosed with KD in 2011 and 2012, was conducted in 2013 and included a total of 1983 departments and hospitals. In order to report on all patients with KD during the 2 survey years, we targeted hospitals of 100 beds or more with pediatric departments, or specialized pediatric hospitals. Results: From a total of 1420 hospitals and departments (71.6% response rate), 26 691 KD patients were reported (12 774 in 2011 and 13 917 in 2012 15 442 males and 11 249 females). The annual incidence rates were 243.1 per 100 000 population aged 0 to 4 years in 2011 and 264.8 in 2012. The number of cases of KD recorded in 2012 was the highest ever reported in Japan. The incidence rate of complete cases was also the highest ever reported in Japan and contributed to the increase in the rate of total cases in recent years. The number of patients diagnosed per month peaked in January, and additional peaks were noted during summer months, although these peaks were lower than those seen in winter. Age-specific incidence rate showed a monomodal distribution with a peak in the latter half of the year in which patients were born. Conclusions: The number of patients and the incidence rate of KD in Japan continue to increase. A similar trend has also been seen for patients with complete KD.
  • Nobuko Makino, Seiji Madoiwa, Tsukasa Ohmori, Kazuo Katoh, Shigeo Ookawara, Takeharu Kanazawa, Osamu Matsuo, Masumi Ichikawa, Jun Mimuro, Keiichi Ichimura, Yoichi Sakata
    INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY 3 6 458 - 467 2013年06月 [査読有り][通常論文]
     
    Background Tissue type plasminogen activator (tPA) functions as a fibrinolytic factor in the blood and has unique roles in the nervous system. However, the role of tPA in the olfactory epithelium (OE) is still unclear. Generally, surgical ablation of the olfactory bulb (bulbectomy) triggers degeneration followed by regeneration of OE. In this experimental study, we investigated the role of tPA in OE regeneration. Methods Wild-type (WT) mice and tPA-knockout (tPA-/-) mice were subjected to bulbectomy. Reverse-transcription polymerase chain reaction (RT-PCR), in situ hybridization, and immunohistochemical examination was done to detect tPA expression in the olfactory bulb and OE. Cellular proliferation and apoptosis was also monitored in the OE. Results Before bulbectomy, tPA was found to be expressed in the olfactory bulb and OE. OE degenerated to a similar extent in both strains between 0 and 3 days after bulbectomy. However, OE was thicker and contained more cells in tPA-/- mice than in WT mice at 7 days after bulbectomy. Moreover, the number of apoptotic bodies was reduced and the number of proliferating cells was increased in the OE of tPA-/- mice compared to WT mice, after bulbectomy. Transmission electron microscopy revealed continuous degeneration of the OE for up to 7 days after bulbectomy in WT mice. In contrast, we observed some intact olfactory vesicles and almost normal supporting cells in the OE of tPA-/- mice, at 7 days after bulbectomy. Conclusion The current findings show that the tPA-plasmin system plays an inhibitory role in the regulation of regeneration in the OE.
  • Dokai M, Madoiwa S, Yasumoto A, Kashiwakura Y, Ishiwata A, Sakata A, Makino N, Ohmori T, Mimuro J, Sakata Y
    Thrombosis research 128 3 283 - 292 2011年09月 [査読有り][通常論文]
     
    Neutrophil elastase released from activated neutrophils contributes in combating bacterial infection. While chronic inflammation results in anemia and decreased bone marrow activities, little is known about the effect of neutrophil elastase on hematological cell growth in severe inflammatory states. Here, we demonstrated that alpha 1-antitrypsin, a physiological inhibitor of neutrophil elastase, functions as a regulator for cell growth by neutralizing neutrophil elastase activity in lipopolysaccharide-primed hematological cells. HL-60 cells were resistant to neutrophil elastase, as they also expressed alpha 1-antitrypsin. The growth of HL-60 cells transduced with a LentiLox-short hairpin alpha 1-antitrypsin vector was significantly suppressed by neutrophil elastase or lipopolysaccharide. When CD34(+) progenitor cells were differentiated towards a granulocytic lineage, they concomitantly expressed neutrophil elastase and alpha 1-antitrypsin and prevented neutrophil elastase-induced growth inhibition. These results suggest that granulocytes might protect themselves from neutrophil elastase-induced cellular damage by efficiently neutralizing its activity through the simultaneous secretion of endogenous alpha 1-antitrypsin. (C) 2011 Elsevier Ltd. All rights reserved.
  • Nobuko Makino, Shigeo Ookawara, Kazuo Katoh, Yasushi Ohta, Masumi Ichikawa, Keiichi Ichimura
    CHEMICAL SENSES 34 2 171 - 179 2009年02月 [査読有り][通常論文]
     
    Transmission electron microscopy was used to study the responses of the supporting cells of the olfactory epithelium at 1-5 days after surgical ablation of the olfactory bulb (bulbectomy). In intact olfactory epithelium, lamellar smooth endoplasmic reticulum and rod-shaped mitochondria were distinctly observed in the supporting cells. On the first day after bulbectomy, bending of the microvilli and an increase in the smooth endoplasmic reticulum were observed. Cristae of the mitochondria became obscure, and the density of the mitochondrial matrix decreased. On the second day after bulbectomy, the number of microvilli decreased, broad cytoplasmic projections that contained cytoplasmic organelles protruded into the luminal side, and the mitochondria were swollen. On the fifth day after bulbectomy, microvilli seemed to be normal and some cells had large cytoplasmic projections that protruded toward the lumen of the nasal cavity. Within the cytoplasmic projections of the supporting cells, a large lamellar and reticular-shaped smooth endoplasmic reticulum was evident. Mitochondria exhibited almost normal morphology. The current findings demonstrate that morphological changes occur in the supporting cells after bulbectomy. This new evidence hypothesizes that these changes represent events that contribute to the regeneration of the olfactory epithelium after bulbectomy.
  • Y Ohta, M Takanosawa, C Matumoto, N Makino, S Ishimoto, K Ichimura
    ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY 111 10 909 - 911 2002年10月 [査読有り][通常論文]
     
    Glucocorticoids (GCs) are commonly prescribed for treatment of olfactory dysfunction. However, the effects of GCs on olfactory epithelium are not well known. We investigated the effects of high-dose GCs on proliferating cells of olfactory epithelium. Five adult male rats (300 g) received a single daily subcutaneous dose of vehicle containing 0.3 mg dexamethasone (DEX) for 9 days (DEX+ group), and a control group received vehicle alone (DEX- group). We compared sections from the two groups for numbers of Ki67-positive cells. The mean number of Ki67-positive cells per 500 olfactory epithelial cells was 9.6 for the DEX+ group and 58 for the DEX- group (significant difference). We conclude that high-dose GC suppressed proliferation of olfactory epithelium. We suggest that high-dose GC suppresses cytokines and growth factors, resulting in secondary suppression of proliferating ability.

講演・口頭発表等

  • Epidemiology of Kawasaki disease in Japan: The etiology of Kawasak disease investigated from epidemiology  [招待講演]
    牧野伸子
    2017国際疫学会 2017年08月 シンポジウム・ワークショップパネル(指名)

MISC

  • 窓岩 清治, 牧野 伸子, 大森 司, 三室 淳, 坂田 洋一 日本血栓止血学会誌 24 (5) 485 -490 2013年 [査読無し][通常論文]
  • 西野 宏, 高野澤 美奈子, 太田 康, 石川 敏夫, 木村 宙倫, 池田 佐恵子, 瀬嶋 尊之, 牧野 伸子, 斎藤 知寿, 市村 恵一 日本鼻科学会会誌 47 (1) 16 -20 2008年04月 [査読無し][通常論文]
  • 阿部 弘一, 西野 宏, 牧野 伸子, 石川 和宏, 石川 浩太郎, 今井 恵子, 市村 恵一 日本耳鼻咽喉科學會會報 110 (1) 13 -19 2007年 [査読無し][通常論文]
     
    気管挿管の合併症や喉頭外傷の一つとして披裂軟骨脱臼がある. これらは自然回復例もあるが, 全身麻酔やNLA麻酔のもとに直達喉頭鏡を挿入しての整復術の報告が多く, また喉頭形成術など観血的手術が必要となった症例も報告されている. 今回我々は8例の披裂軟骨脱臼症例を経験し, 自然回復例を除く5例に対して局所麻酔下の整復術を外来手術として施行し良好な結果を得た. 症例は53~75歳の男性2例, 女性3例である. すべて前方型脱臼で4例が気管挿管後, 1例がラリンゲアルマスク使用例であった. 整復術は, 両鼻腔および喉頭に4%キシロカイン®で表面麻酔を行った後, 一側鼻腔よりファイバースコープを他側鼻腔より尿道バルーンカテーテルを挿入して行った. 喉頭を観察しながら, バルーンを拡張させ披裂部の高さで数秒間留置する操作 (留置法) とバルーンを声門部で拡張させ引き抜く操作 (引き抜き法) を行った. これらを数回繰り返して1回の整復術とした. 3例は1回目の整復術後1~2週で改善したが, 2例は改善が不十分であり2回目を追加した. この2例も2回目の整復術後1~2週で声帯運動, 嗄声ともに改善した. なお, 5症例に対し計7回の整復術を施行したが全例で合併症は認められなかった.本法は外来で施行が可能であり, 簡便で侵襲も少なく成績も良好であるため, 前方型披裂軟骨脱臼症に対する治療法として有用と考えられた.
  • 太田 康, 椿 恵樹, 山本 昌範, 児玉 梢, 牧野 伸子, 石川 敏夫, 市村 恵一 日本耳鼻咽喉科學會會報 109 (3) 157 -162 2006年 [査読無し][通常論文]
     
    Chronic sinusitis with eosinophils easily recurs after endoscopic sinus surgery. The condition is usually complicated by asthma, and many eosinophils are present in the sinus mucosa. One conservative treatment method is the administration of glucocorticoids locally or systematically. To evaluate the efficacy and tolerability of intranasal fluticasone propionate for the treatment of chronic sinusitis with eosinophils, seven patients with chronic sinusitis with eosinophils were treated over a 12-week period using a fluticasone propionate aqueous nasal spray (800μg per day in each nostrilz). The Symptons of 7 patients, especially nasal discharge and nasal obstructions, improved and an expanded air space was observed on paranasal CT images. The percent of drug systemically available after intranasal administration varied by less than 1% for intranasal fluticasone propionate. Therefore, even if intranasal fluticasone propionate is administerved at double the usual dose, it is unlikely to cause systemic side effects. Fluticasone propionate aqueous nasal spray at double the usual dose is effective for the treatment of chronic sinusitis with eosinophils.
  • 牧野 伸子, 太田 康, 石川 敏夫, 市村 恵一 日本鼻科学会会誌 44 (2) 123 -126 2005年08月 [査読無し][通常論文]
  • 牧野 伸子, 太田 康, 石川 敏夫, 市村 恵一 日本耳鼻咽喉科學會會報 108 (5) 528 -532 2005年 [査読無し][通常論文]
     
    Purpose: To evaluate the systemic effects of intranasal steroid drops in patients with dysosmia.<BR>Cases and Methods: Intranasal steroid drops were administered for 12 weeks to 23 patients with dysosmia, and their plasma cortisol and adrenocorticotropic hormone (ACTH) values were measured before and after treatment. Improvement in dysosmia was judged based on the standerd olfactory test and the patients' symptoms.<BR>Results: The post-treatment plasma cortisol levels ranged from 0.1 to 25.0μg/dl (5.4±5.9μg/dl), and the posttreatment plasma ACTH levels ranged from 5.0 to 55.0pg/ml (13.8±11.7pg/ml). After treatment, the 23 cases were divided into two groups: 14 cases (60.9%) with a decline in cortisol and/or ACTH level, and 9 patients (39.1%) with normal plasma cortisol and ACTH levels. Improvement in dysosmia was found in 4 patients (28.6%) in the former group and 4 patients (44.4%) in the latter group. The difference in percentage of patients who improved was not significant between the groups.<BR>Conclusions: We conclude that the direct topical effect of intranasal steroid drops on the olfactory mucosa may have been the principal reason for the improvement in the patients' dysosmia.
  • 市村 恵一, 太田 康, 丹生 健一, 石川 敏夫, 高野澤 美奈子, 牧野 伸子, 安井 理絵, 近藤 健二, 松本 千織 日本鼻科学会会誌 43 (1) 92 -94 2004年04月 [査読無し][通常論文]
  • 市村 恵一, 石川 和宏, 高野澤 美奈子, 瀬嶋 尊之, 佐藤 貴恵, 牧野 伸子, 石川 敏夫, 太田 康, 石田 孝 日本鼻科学会会誌 42 (2) 138 -145 2003年08月 [査読無し][通常論文]
  • 市村 恵一, 瀬嶋 尊之, 太田 康, 牧野 伸子 日本鼻科学会会誌 41 (2) 149 -155 2002年06月 [査読無し][通常論文]
  • 牧野 伸子, 太田 康, 市村 恵一 日本鼻科学会会誌 41 (2) 162 -166 2002年06月 [査読無し][通常論文]


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