Researchers Database

sato yusuke

    DepartmentofInfectionandImmunityDivisionofBacteriology Research Associate
Last Updated :2021/11/23

Researcher Information

J-Global ID

Research Interests

  • 合成生物学   薬剤耐性   ファージセラピー   Bacteriophage   Toxin   Pathogenic   Staphylococcus aureus   Bacteriology   

Research Areas

  • Life sciences / Bacteriology

Academic & Professional Experience

  • 2015/04 - Today  Jichi medical universitydivision of bacteriologyAssistant professor
  • 2015/04 - Today  Jichi Medical University感染免疫学講座細菌学助教
  • 2014/10 - 2015/03  Hiroshma universityBacteriologyResearcher
  • 2014/10 - 2015/03  Hiroshima University医歯薬保健学研究科細菌学研究員

Education

  • 2012/10 - 2014/09  Hirosima university
  • 2012/10 - 2014/09  Hiroshima University  Graduate School of Biomedical Sciences  医歯薬保健学研究科
  • 2011/04 - 2014/09  Gifu gruduated university  Department of veterinary science
  • 2011/03 - 2014/09  Gifu University  The United Graduate School of Veterinary Sciences  連合獣医学研究科
  • 2005/04 - 2011/03  Iwate university  Depertment of agriculture  Veterinary medicine
  •        -   Iwate University  Faculty of Agriculture  獣医学科

Association Memberships

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN   日本ブドウ球菌研究会   JAPANESE SOCIETY FOR BACTERIOLOGY   JAPANESE SOCIETY OF VETERINARY SCIENCE   JAPAN VETERINARY MEDICAL ASSOCIATION   栃木県獣医師会   

Published Papers

  • Harumi Koibuchi, Yasutomo Fujii, Yusuke Sato’o, Takashi Mochizuki, Toshiyuki Yamada, Longzhu Cui, Nobuyuki Taniguchi
    Journal of Medical Ultrasonics 1346-4523 2021/08 
    Abstract Purpose We aimed to investigate whether low-intensity continuous and pulsed wave ultrasound (US) irradiation can inhibit the formation of Staphylococcus epidermidis biofilms, for potential application in the treatment of catheter-related bloodstream infections (CRBSI). Methods S. epidermidis biofilms that formed on the bottom surfaces of 6-well plates were irradiated on the bottom surface using the sound cell incubator system for different intervals of time. Results US irradiation with continuous waves for 24 h notably inhibited biofilm formation (p < 0.01), but the same US irradiation for 12 h had no remarkable effect. Further, double US irradiation with pulsed waves for 20 min inhibited biofilm formation by 33.6%, nearly two-fold more than single US irradiation, which reduced it by 17.9%. Conclusion US irradiation of a lower intensity (ISATA = 6–29 mW/cm2) than used in a previous study and lower than recommended by the Food and Drug Administration shows potential for preventing CRBSI caused by bacterial biofilms.
  • Yusuke SATO’O, Katsuhiko OMOE, Yasuko AIKAWA, Mayuko KANO, Hisaya K. ONO, Dong-Liang HU, Akio NAKANE, Motoyuki SUGAI
    Journal of Veterinary Medical Science 0916-7250 2021 [Refereed]
  • Kanate Thitiananpakorn, Yoshifumi Aiba, Xin-Ee Tan, Shinya Watanabe, Kotaro Kiga, Yusuke Sato’o, Tanit Boonsiri, Feng-Yu Li, Teppei Sasahara, Yusuke Taki, Aa Haeruman Azam, Yuancheng Zhang, Longzhu Cui
    Scientific Reports 10 (1) 16107 - 16107 2020/12 [Refereed][Not invited]
     
    Abstract We first reported a phenomenon of cross-resistance to vancomycin (VCM) and daptomycin (DAP) in methicillin-resistant Staphylococcus aureus (MRSA) in 2006, but mechanisms underlying the cross-resistance remain incompletely understood. Here, we present a follow-up study aimed to investigate genetic determinants associated with the cross-resistance. Using 12 sets of paired DAP susceptible (DAPS) and DAP non-susceptible (DAPR) MRSA isolates from 12 patients who had DAP therapy, we (i) assessed susceptibility to DAP and VCM, (ii) compared whole-genome sequences, (iii) identified mutations associated with cross-resistance to DAP and VCM, and (iv) investigated the impact of altered gene expression and metabolic pathway relevant to the cross-resistance. We found that all 12 DAPR strains exhibiting cross-resistance to DAP and VCM carried mutations in mprF, while one DAPR strain with reduced susceptibility to only DAP carried a lacF mutation. On the other hand, among the 32 vancomycin-intermediate S. aureus (VISA) strains isolated from patients treated with VCM, five out of the 18 strains showing cross-resistance to DAP and VCM carried a mprF mutation, while 14 strains resistant to only VCM had no mprF mutation. Moreover, substitution of mprF in a DAPS strain with mutated mprF resulted in cross-resistance and vice versa. The elevated lysyl-phosphatidylglycerol (L-PG) production, increased positive bacterial surface charges and activated cell wall (CW) synthetic pathways were commonly found in both clinical isolates and laboratory-developed mutants that carry mprF mutations. We conclude that mprF mutation is responsible for the cross-resistance of MRSA to DAP and VCM, and treatment with DAP is more likely to select for mprF-mediated cross-resistance than is with VCM.
  • Tanit Boonsiri, Shinya Watanabe, Xin-Ee Tan, Kanate Thitiananpakorn, Ryu Narimatsu, Kosuke Sasaki, Remi Takenouchi, Yusuke Sato’o, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, Yusuke Taki, Feng-Yu Li, Yuancheng Zhang, Aa Haeruman Azam, Tomofumi Kawaguchi, Longzhu Cui
    Scientific Reports 10 (1) 16907 - 16907 2020/12 [Refereed]
     
    Abstract Staphylococcus aureus strains that are susceptible to the β-lactam antibiotic oxacillin despite carrying mecA (OS-MRSA) cause serious clinical problems globally because of their ability to easily acquire β-lactam resistance. Understanding the genetic mechanism(s) of acquisition of the resistance is therefore crucial for infection control management. For this purpose, a whole-genome sequencing-based analysis was performed using 43 clinical OS-MRSA strains and 100 mutants with reduced susceptibility to oxacillin (MICs 1.0–256 µg/mL) generated from 26 representative OS-MRSA strains. Genome comparison between the mutants and their respective parent strains identified a total of 141 mutations in 46 genes and 8 intergenic regions. Among them, the mutations are frequently found in genes related to RNA polymerase (rpoBC), purine biosynthesis (guaA, prs, hprT), (p)ppGpp synthesis (relSau), glycolysis (pykA, fbaA, fruB), protein quality control (clpXP, ftsH), and tRNA synthase (lysS, gltX), whereas no mutations existed in mec and bla operons. Whole-genome transcriptional profile of the resistant mutants demonstrated that expression of genes associated with purine biosynthesis, protein quality control, and tRNA synthesis were significantly inhibited similar to the massive transcription downregulation seen in S. aureus during the stringent response, while the levels of mecA expression and PBP2a production were varied. We conclude that a combination effect of mecA upregulation and stringent-like response may play an important role in acquisition of β-lactam resistance in OS-MRSA.
  • Kotaro Kiga, Xin-Ee Tan, Rodrigo Ibarra-Chávez, Shinya Watanabe, Yoshifumi Aiba, Yusuke Sato’o, Feng-Yu Li, Teppei Sasahara, Bintao Cui, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Aa Haeruman Azam, Masato Suzuki, José R. Penadés, Longzhu Cui
    Nature Communications 11 (1) 2020/12 [Refereed][Not invited]
     
    AbstractThe emergence of antimicrobial-resistant bacteria is an increasingly serious threat to global health, necessitating the development of innovative antimicrobials. Here we report the development of a series of CRISPR-Cas13a-based antibacterial nucleocapsids, termed CapsidCas13a(s), capable of sequence-specific killing of carbapenem-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus by recognizing corresponding antimicrobial resistance genes. CapsidCas13a constructs are generated by packaging programmed CRISPR-Cas13a into a bacteriophage capsid to target antimicrobial resistance genes. Contrary to Cas9-based antimicrobials that lack bacterial killing capacity when the target genes are located on a plasmid, the CapsidCas13a(s) exhibit strong bacterial killing activities upon recognizing target genes regardless of their location. Moreover, we also demonstrate that the CapsidCas13a(s) can be applied to detect bacterial genes through gene-specific depletion of bacteria without employing nucleic acid manipulation and optical visualization devices. Our data underscore the potential of CapsidCas13a(s) as both therapeutic agents against antimicrobial-resistant bacteria and nonchemical agents for detection of bacterial genes.
  • Yoshifumi Aiba, Shinya Watanabe, Rieko Tsukahara, Naoka Umemoto, Kanate Thitiananpakorn, Tanit Boonsiri, Feng-Yu Li, Kotaro Kiga, Yusuke Sato'o, Xin-Ee Tan, Yusuke Taki, Aa Haeruman Azam, Yuancheng Zhang, Teppei Sasahara, Toshio Demitsu, Longzhu Cui
    Microbiology resource announcements 9 (23) 2020/06 [Refereed][Not invited]
     
    The association of Panton-Valentine leukocidin (PVL) toxin with necrotizing soft tissue infection (NSTI) caused by Staphylococcus aureus remains controversial. Here, we report the complete genome sequence of the PVL-negative S. aureus strain JMUB1273, isolated from a patient with pervasive NSTI.
  • Shinya Watanabe, Bintao Cui, Kotaro Kiga, Yoshifumi Aiba, Xin Ee Tan, Yusuke Sato'o, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Fen Yu Li, Aa Haeruman Azam, Yumi Nakada, Teppei Sasahara, Longzhu Cui
    Frontiers in Microbiology 11 2020/02 
    In the original article, there was a mistake in Table 1 as published. “GC% of L. wadei JMUB3933, JMUB3934, JCM16777, Leptotrichia sp.-1 JMUB3936, L. shahii JCM16776, L. hofstadii JCM16775, L. trevisanii JMUB3870, JMUB4039, JMUB3935 and L. buccalis C-1013-b, Leptotrchia sp.-3 F0260, Leptotrichia sp. F0590, L. goodfellowi JCM16774 and Leptotrichia sp.-6W10393, and chromosome length of L. wadei JCM16777” were incorrect. The corrected Table 1 appears below. In the original article, there was an error. GC% of genome-sequenced strains was incorrect. A correction has been made to Results and Discussion, Comparative Analysis of Leptotrichia Genome, line 373-375: As shown in Table 1, the chromosome size of the genus Leptotrichia varies from 2,142,946 to 2,829,322 bp with GC contents of 29.5% to 31.7%. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
  • Fatkhanuddin Aziz, Junzo Hisatsune, Liansheng Yu, Junko Kajimura, Yusuke Sato'o, Hisaya K Ono, Kanako Masuda, Mika Yamaoka, Siti Isrina Oktavia Salasia, Akio Nakane, Hiroki Ohge, Yoichiro Kusunoki, Motoyuki Sugai
    Infection and immunity 88 (2) 2020/01 [Refereed][Not invited]
     
    While investigating the virulence traits of Staphylococcus aureus adhering to the skin of atopic-dermatitis (AD) patients, we identified a novel open reading frame (ORF) with structural similarity to a superantigen from genome sequence data of an isolate from AD skin. Concurrently, the same ORF was identified in a bovine isolate of S. aureus and designated SElY (H. K. Ono, Y. Sato'o, K. Narita, I. Naito, et al., Appl Environ Microbiol 81:7034-7040, 2015, https://doi.org/10.1128/AEM.01873-15). Recombinant SElYbov had superantigen activity in human peripheral blood mononuclear cells. It further demonstrated emetic activity in a primate animal model, and it was proposed that SElY be renamed SEY (H. K. Ono, S. Hirose, K. Narita, M. Sugiyama, et al., PLoS Pathog 15:e1007803, 2019, https://doi.org/10.1371/journal.ppat.1007803). Here, we investigated the prevalence of the sey gene in 270 human clinical isolates of various origins in Japan. Forty-two strains were positive for the sey gene, and the positive isolates were from patients with the skin diseases atopic dermatitis and impetigo/staphylococcal scalded skin syndrome (SSSS), with a detection rate of ∼17 to 22%. There were three variants of SEY (SEY1, SEY2, and SEY3), and isolates producing SEY variants formed three distinct clusters corresponding to clonal complexes (CCs) 121, 59, and 20, respectively. Most sey+ isolates produced SEY in broth culture. Unlike SEYbov, the three recombinant SEY variants exhibited stability against heat treatment. SEY predominantly activated human T cells with a particular T-cell receptor (TCR) Vα profile, a unique observation since most staphylococcal enterotoxins exert their superantigenic activities through activating T cells with specific TCR Vβ profiles. SEY may act to induce localized inflammation via skin-resident T-cell activation, facilitating the pathogenesis of S. aureus infection in disrupted epithelial barriers.
  • Shinya Watanabe, Bintao Cui, Kotaro Kiga, Yoshifumi Aiba, Xin-Ee Tan, Yusuke Sato'o, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Fen-Yu Li, Aa Haeruman Azam, Yumi Nakada, Teppei Sasahara, Longzhu Cui
    Frontiers in microbiology 10 2838 - 2838 2019 [Not refereed][Not invited]
     
    Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a, previously known as CRISPR-C2c2, is the most recently identified RNA-guided RNA-targeting CRISPR-Cas system that has the unique characteristics of both targeted and collateral single-stranded RNA (ssRNA) cleavage activities. This system was first identified in Leptotrichia shahii. Here, the complete whole genome sequences of 11 Leptotrichia strains were determined and compared with 18 publicly available Leptotrichia genomes in regard to the composition, occurrence and diversity of the CRISPR-Cas13a, and other CRISPR-Cas systems. Various types of CRISPR-Cas systems were found to be unevenly distributed among the Leptotrichia genomes, including types I-B (10/29, 34.4%), II-C (1/29, 2.6%), III-A (6/29, 15.4%), III-D (6/29, 15.4%), III-like (3/29, 7.7%), and VI-A (11/29, 37.9%), while 8 strains (20.5%) had no CRISPR-Cas system at all. The Cas13a effectors were found to be highly divergent with amino acid sequence similarities ranging from 61% to 90% to that of L. shahii, but their collateral ssRNA cleavage activities leading to impediment of bacterial growth were conserved. CRISPR-Cas spacers represent a sequential achievement of former intruder encounters, and the retained spacers reflect the evolutionary phylogeny or relatedness of strains. Analysis of spacer contents and numbers among Leptotrichia species showed considerable diversity with only 4.4% of spacers (40/889) were shared by two strains. The organization and distribution of CRISPR-Cas systems (type I-VI) encoded by all registered Leptotrichia species revealed that effector or spacer sequences of the CRISPR-Cas systems were very divergent, and the prevalence of types I, III, and VI was almost equal. There was only one strain carrying type II, while none carried type IV or V. These results provide new insights into the characteristics and divergences of CRISPR-Cas systems among Leptotrichia species.
  • Bintao Cui, Shinya Watanabe, Yusuke Sato'o, Fumiya Nihashi, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, Xin-Ee Tan, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Feng-Yu Li, Shiro Imokawa, Longzhu Cui
    Microbiology resource announcements 8 (4) e01652-18  2019/01 [Refereed][Not invited]
     
    Severe community-acquired pneumonia (CAP) caused by methicillin-resistant Staphylococcus aureus (MRSA) is relatively rare and is usually associated with rapid progression to death. Here, we report the complete genome sequence of the MRSA strain JMUB3031, which was isolated from a patient with fatal CAP.
  • Shinya Watanabe, Yoshifumi Aiba, Xin-Ee Tan, Feng-Yu Li, Tanit Boonsiri, Kanate Thitiananpakorn, Bintao Cui, Yusuke Sato'o, Kotaro Kiga, Teppei Sasahara, Longzhu Cui
    BMC genomics 19 (1) 810 - 810 2018/11 [Refereed][Not invited]
     
    BACKGROUND: Staphylococcus caprae is an animal-associated bacterium regarded as part of goats' microflora. Recently, S. caprae has been reported to cause human nosocomial infections such as bacteremia and bone and joint infections. However, the mechanisms responsible for the development of nosocomial infections remain largely unknown. Moreover, the complete genome sequence of S. caprae has not been determined. RESULTS: We determined the complete genome sequences of three methicillin-resistant S. caprae strains isolated from humans and compared these sequences with the genomes of S. epidermidis and S. capitis, both of which are closely related to S. caprae and are inhabitants of human skin capable of causing opportunistic infections. The genomes showed that S. caprae JMUB145, JMUB590, and JMUB898 strains contained circular chromosomes of 2,618,380, 2,629,173, and 2,598,513 bp, respectively. JMUB145 carried type V SCCmec, while JMUB590 and JMUB898 had type IVa SCCmec. A genome-wide phylogenetic SNP tree constructed using 83 complete genome sequences of 24 Staphylococcus species and 2 S. caprae draft genome sequences confirmed that S. caprae is most closely related to S. epidermidis and S. capitis. Comparative complete genome analysis of eight S. epidermidis, three S. capitis and three S. caprae strains revealed that they shared similar virulence factors represented by biofilm formation genes. These factors include wall teichoic acid synthesis genes, poly-gamma-DL-glutamic acid capsule synthesis genes, and other genes encoding nonproteinaceous adhesins. The 17 proteinases/adhesins and extracellular proteins known to be associated with biofilm formation in S. epidermidis were also conserved in these three species, and their biofilm formation could be detected in vitro. Moreover, two virulence-associated gene clusters, the type VII secretion system and capsular polysaccharide biosynthesis gene clusters, identified in S. aureus were present in S. caprae but not in S. epidermidis and S. capitis genomes. CONCLUSION: The complete genome sequences of three methicillin-resistant S. caprae isolates from humans were determined for the first time. Comparative genome analysis revealed that S. caprae is closely related to S. epidermidis and S. capitis at the species level, especially in the ability to form biofilms, which may lead to increased virulence during the development of S. caprae infections.
  • Yusuke Sato'o, Yoshifumi Aiba, Kotaro Kiga, Shinya Watanabe, Teppei Sasahara, Yasuhiko Hayakawa, Longzhu Cui
    Journal of microbiological methods 146 25 - 32 0167-7012 2018/03 [Refereed][Not invited]
     
    Electroporation is a common technique necessary for genomic manipulation of Staphylococci. However, because this technique has too low efficiency to be applied to some Staphylococcal species and strains, especially to coagulase-negative Staphylococcus (CNS) isolates, basic researches on these clinically important Staphylococci are limited. Here we report on the optimization of electroporation parameters and conditions as well as on the generation of a universal protocol that can be efficiently applicable to both CNS and Coagulase-positive Staphylococci (CPS). This protocol could generate transformants of clinical Staphylococcus epidermidis isolate, with an efficiency of up to 1400 CFU/μg of plasmid DNA. Transformants of 12 other clinically important Staphylococcal species, including CNS and CPS, were also generated with this protocol. To our knowledge, this is the first report on successful electroporation in nine these Staphylococcal species.
  • Yusuke Sato’o, Junzo Hisatsune, Liansheng Yu, Tetsushi Sakuma, Takashi Yamamoto, Motoyuki Sugai
    PLoS ONE 13 (1) e0185987  1932-6203 2018/01 [Refereed][Not invited]
     
    Preparing the genetically modified organisms have required much time and labor, making it the rate-limiting step but CRISPR/Cas9 technology appearance has changed this difficulty. Although reports on CRISPR/Cas9 technology such as genome editing and CRISPR interference (CRISPRi) in eukaryotes increased, those in prokaryotes especially in Staphylococci were limited. Thus, its potential in the bacteriology remains unexplored. This is attributed to ecological difference between eukaryotes and prokaryotes. Here, we constructed a novel CRISPRi plasmid vector, pBACi for Staphylococcus aureus. The transformation efficiency of S. aureus was ~104 CFU/μg DNA using a vector extracted from dcm negative, which encoded one of DNA modification genes, E. coli. Further, pBACi was introduced into various clinical isolates including that not accepting the conventional temperature-sensitive vector. dcas9 in the vector was expressed throughout the growth phases of S. aureus and this vector decreased various gene mRNA expressions based on the crRNA targeting sequences and altered the knockdown strains’ phenotypes. The targeted genes included various virulence and antibiotic resistant genes. Bioinformatics suggest this vector can be introduced into wide range of low-GC Gram-positive bacteria. Because this new CRISPR/Cas9-based vector can easily prepare knockdown strains, we believe the novel vector will facilitate the characterization of the function of genes from S. aureus and other Gram-positive bacteria.
  • Hisaya K. Ono, Shouhei Hirose, Ikunori Naito, Yusuke Sato'o, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Akio Nakane
    MICROBIOLOGY AND IMMUNOLOGY 61 (1) 12 - 16 0385-5600 2017/01 [Refereed][Not invited]
     
    Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable causative agents of emetic food poisoning in humans. New types of SEs and SE-like (SEl) toxins have been reported. Several epidemiological investigations have shown that the SEs and SEl genes, particularly, SEK, SEL, SEM, SEN and SEO genes, are frequently detected in strains isolated from patients with food poisoning. The purpose of the present study was to evaluate the emetic activity of recently identified SEs using a small emetic animal model, the house musk shrew. The emetic activity of these SEs in house musk shrews was evaluated by intraperitoneal administration and emetic responses, including the number of shrews that vomited, emetic frequency and latency of vomiting were documented. It was found that SEs induce emetic responses in these animals. This is the first time to demonstrate that SEK, SEL, SEM, SEN and SEO possess emetic activity in the house musk shrew.
  • Liansheng Yu, Junzo Hisatsune, Ikue Hayashi, Nobuyuki Tatsukawa, Yusuke Sato'o, Emiri Mizumachi, Fuminori Kato, Hideki Hirakawa, Gerald B. Pier, Motoyuki Sugai
    MBIO 8 (1) 2150-7511 2017/01 [Refereed][Not invited]
     
    Staphylococcus aureus TF2758 is a clinical isolate from an atheroma and a super-biofilm-elaborating/polysaccharide intercellular adhesin (PIA)/poly-Nacetylglucosamine (PNAG)-overproducing strain (L. Shrestha et al., Microbiol Immunol 60: 148 -159, 2016, https://doi.org/10.1111/1348-0421.12359). A microarray analysis and DNA genome sequencing were performed to identify the mechanism underlying biofilm overproduction by TF2758. We found high transcriptional expression levels of a 7-gene cluster (satf2580 to satf2586) and the ica operon in TF2758. Within the 7-gene cluster, a putative transcriptional regulator gene designated rob had a nonsense mutation that caused the truncation of the protein. The complementation of TF2758 with rob from FK300, an rsbU-repaired derivative of S. aureus strain NCTC8325-4, significantly decreased biofilm elaboration, suggesting a role for rob in this process. The deletion of rob in non-biofilm-producing FK300 significantly increased biofilm elaboration and PIA/PNAG production. In the search for a gene(s) in the 7-gene cluster for biofilm elaboration controlled by rob, we identified open reading frame (ORF) SAOUHSC_ 2898 (satf2584). Our results suggest that ORF SAOUHSC_ 2898 (satf2584) and icaADBC are required for enhanced biofilm elaboration and PIA/PNAG production in the rob deletion mutant. Rob bound to a palindromic sequence within its own promoter region. Furthermore, Rob recognized the TATTT motif within the icaR-icaA intergenic region and bound to a 25-bp DNA stretch containing this motif, which is a critically important short sequence regulating biofilm elaboration in S. aureus. Our results strongly suggest that Rob is a longsought repressor that recognizes and binds to the TATTT motif and is an important regulator of biofilm elaboration through its control of SAOUHSC_ 2898 (SATF2584) and Ica protein expression in S. aureus. IMPORTANCE During the search for molecular mechanisms underlying biofilm overproduction of Staphylococcus aureus TF2758, we found a putative transcriptional regulator gene designated rob within a 7-gene cluster showing a high transcriptional expression level by microarray analysis. The deletion of rob in non-biofilm-producing FK300, an rsbU-repaired derivative of NCTC8325-4, significantly increased biofilm elaboration and PIA/PNAG production. The search for a gene(s) in the 7-gene cluster for biofilm elaboration controlled by rob identified ORF SAOUHSC_ 2898. Besides binding to its own promoter region to control ORF SAOUHSC_ 2898 expression, Rob recognized the TATTT motif within the icaR-icaA intergenic region and bound to a 25-bp DNA stretch containing this motif, which is a critically important short sequence regulating biofilm elaboration in S. aureus. Our results strongly suggest that Rob is a long-sought repressor that recognizes and binds to the TATTT motif and is a new important regulator of biofilm elaboration through its control of SAOUHSC_2898 and Ica protein expression in S. aureus.
  • Yusuke Sato'o, Junzo Hisatsune, Hideki Hirakawa, Hisaya K. Ono, Katsuhiko Omoe, Motoyuki Sugai
    Genome Announcements 5 (34) 2169-8287 2017 [Refereed][Not invited]
     
    Staphylococcus aureus No. 10 is an isolate from a staphylococcal food poisoning outbreak in Japan, classified as clonal complex 81 subtype 1. It preferentially produces larger quantities of staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin H (SEH) in foods and media. Here, we report the complete annotated genome sequence of the chromosome and a plasmid.
  • Junzo Hisatsune, Yusuke Sato'o, Liansheng Yu, Shoko Kutsuno, Yasuhiko Hayakawa, Motoyuki Sugai
    JOURNAL OF MICROBIOLOGICAL METHODS 130 69 - 72 0167-7012 2016/11 [Refereed][Not invited]
     
    A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9 x 10(5) S. aureus RN4220 transformed cells/1 mu g plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse. (C) 2016 Elsevier B.V. All rights reserved.
  • Shinya Watanabe, Teppei Sasahara, Naoshi Arai, Kazumasa Sasaki, Yoshifumi Aiba, Yusuke Sato'o, Longzhu Cui
    Genome Announcements 4 (5) 2169-8287 2016 [Refereed][Not invited]
     
    Acute phlegmonous gastritis is an uncommon endogenous bacterial gastritis presenting with a high mortality rate. Here, we report the complete genome sequence of an emm89 Streptococcus pyogenes strain, JMUB1235, which is the causative agent of acute phlegmonous gastritis.
  • Yusuke Sato'o, Junzo Hisatsune, Yuria Nagasako, Hisaya K. Ono, Katsuhiko Omoe, Motoyuki Sugai
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81 (22) 7782 - 7790 0099-2240 2015/11 [Refereed][Not invited]
     
    We previously demonstrated the clonal complex 81 (CC81) subtype 1 lineage is the major staphylococcal food poisoning (SFP)-associated lineage in Japan (Y. Sato'o et al., J Clin Microbiol 52:2637-2640, 2014, http://dx.doi.org/10.1128/JCM.00661-14). Strains of this lineage produce staphylococcal enterotoxin H (SEH) in addition to SEA. However, an evaluation of the risk for the recently reported SEH has not been sufficiently conducted. We first searched for staphylococcal enterotoxin (SE) genes and SE proteins in milk samples that caused a large SFP outbreak in Japan. Only SEA and SEH were detected, while there were several SE genes detected in the samples. We next designed an experimental model using a meat product to assess the productivity of SEs and found that only SEA and SEH were detectably produced in situ. Therefore, we investigated the regulation of SEH production using a CC81 subtype 1 isolate. Through mutant analysis of global regulators, we found the repressor of toxin (Rot) functioned oppositely as a stimulator of SEH production. SEA production was not affected by Rot. seh mRNA expression correlated with rot both in media and on the meat product, and the Rot protein was shown to directly bind to the seh promoter. The seh promoter sequence was predicted to form a loop structure and to hide the RNA polymerase binding sequences. We propose Rot binds to the promoter sequence of seh and unfolds the secondary structure that may lead the RNA polymerase to bind the promoter, and then seh mRNA transcription begins. This alternative Rot regulation for SEH may contribute to sufficient toxin production by the CC81 subtype 1 lineage in foods to induce SFP.
  • Hisaya K. Ono, Yusuke Sato'o, Kouji Narita, Ikunori Naito, Shouhei Hirose, Junzo Hisatsune, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Motoyuki Sugai, Akio Nakane
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81 (20) 7034 - 7040 0099-2240 2015/10 [Refereed][Not invited]
     
    Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus have superantigenic and emetic activities, which cause toxic shock syndrome and staphylococcal food poisoning, respectively. Our previous study demonstrated that the sequence of SET has a low level of similarity to the sequences of other SEs and exhibits atypical bioactivities. Hence, we further explored whether there is an additional SET-related gene in S. aureus strains. One SET-like gene was found in the genome of S. aureus isolates that originated from a case of food poisoning, a human nasal swab, and a case of bovine mastitis. The deduced amino acid sequence of the SET-like gene showed 32% identity with the amino acid sequence of SET. The SET-like gene product was designated SElY. In the food poisoning and nasal swab isolates, mRNA encoding SElY was highly expressed in the early log phase of cultivation, whereas a high level of expression of this mRNA was found in the bovine mastitis isolate at the early stationary phase. To estimate whether SElY has both superantigenic and emetic activities, recombinant SElY was prepared. Cell proliferation and cytokine production were examined to assess the superantigenic activity of SElY. SElY exhibited superantigenic activity in human peripheral blood mononuclear cells but not in mouse splenocytes. In addition, SElY exhibited emetic activity in house musk shrews after intraperitoneal and oral administration. However, the stability of SElY against heating and pepsin and trypsin digestion was different from that of SET and SEA. From these results, we identified SElY to be a novel staphylococcal emetic toxin.
  • Emeline Laborel-Preneron, Pascale Bianchi, Franck Boralevi, Philippe Lehours, Frederique Fraysse, Fanny Morice-Picard, Motoyuki Sugai, Yusuke Sato'o, Cedric Badiou, Gerard Lina, Anne-Marie Schmitt, Daniel Redoules, Christiane Casas, Christian Davrinche
    PLOS ONE 10 (10) e0141067  1932-6203 2015/10 [Refereed][Not invited]
     
    Interactions between the immune system and skin bacteria are of major importance in the pathophysiology of atopic dermatitis (AD), yet our understanding of them is limited. From a cohort of very young AD children (1 to 3 years old), sensitized to Dermatophagoides pteronyssinus allergens (Der p), we conducted culturomic analysis of skin microbiota, cutaneous transcript profiling and quantification of anti-Der p CD4(+) T cells. This showed that the presence of S. aureus in inflamed skin of AD patients was associated with a high IgE response, increased expression of inflammatory and Th2/Th22 transcripts and the prevalence of a peripheral Th2 anti-Der p response. Monocyte-derived dendritic cells (moDC) exposed to the S. aureus and S. epidermidis secretomes were found to release pro-inflammatory IFN-gamma and anti-inflammatory IL-10, respectively. Allogeneic moDC exposed to the S. aureus secretome also induced the proliferation of CD4(+) T cells and this effect was counteracted by concurrent exposure to the S. epidermidis secretome. In addition, whereas the S. epidermidis secretome promoted the activity of regulatory T cells (Treg) in suppressing the proliferation of conventional CD4(+) T cells, the Treg lost this ability in the presence of the S. aureus secretome. We therefore conclude that S. aureus may cause and promote inflammation in the skin of AD children through concomitant Th2 activation and the silencing of resident Treg cells. Commensals such as S. epidermidis may counteract these effects by inducing the release of IL-10 by skin dendritic cells.
  • Yasunori Suzuki, Makiko Kobayashi, Shigeru Matsushita, Satomi Uehara, Rei Kato, Yusuke Sato'o, Hisaya K. Ono, Kenji Sadamasu, Akemi Kai, Yoichi Kamata
    JOURNAL OF VETERINARY MEDICAL SCIENCE 77 (8) 905 - 911 0916-7250 2015/08 [Refereed][Not invited]
     
    The plasmid is a very well-known mobile genetic element that participates in the acquisition of virulence genes, such as staphylococcal enterotoxins (SEs), via horizontal transfer. SEs are emetic toxins and causative agents in staphylococcal food poisoning (SFP). We herein identified the types of plasmids harbored by seven SFP isolates and examined their production of plasmid-related SE/SEI to determine whether the new types of plasmid-related SE or SE-like (SEI) toxins (i.e. SEIJ and SER) were involved in SFP. These isolates harbored pIB485-like plasmids, and all, except for one isolate, produced SEIJ and SER. The amount of SER produced by each isolate accounted for the highest or second highest percentage of the total amount of SE/SEI produced. These new types of plasmid-related SE/SEIs as well as classical SE may play a role in SFP. The seven isolates were classified into two SED-production types; a high SED-production type (>500 ng/ml) and no SED-production type. A nucleotide sequencing analysis revealed that three plasmids harbored by the SED-non-producing isolates had a single-base deletion in the red gene with a resulting stop codon (from 233 amino acids of the intact SED to 154 amino acids of the mutant SED (mSED)). A real-time reverse transcription-PCR analysis showed that the mRNA of the msed gene was transcribed in the isolates. If the msed gene was translated as a protein, mSED may act as an emetic toxin instead of intact SED.
  • Y. Suzuki, H. Kubota, Y. Sato'o, H. K. Ono, R. Kato, K. Sadamasu, A. Kai, Y. Kamata
    JOURNAL OF APPLIED MICROBIOLOGY 118 (6) 1507 - 1520 1364-5072 2015/06 [Refereed][Not invited]
     
    AimsHorizontal transfer of Staphylococcus aureus pathogenicity islands (SaPIs) plays an important role in acquiring pathogenicity. This study aimed to identify novel SaPIs encoding staphylococcal enterotoxins (SEs) and to characterize their SE productivity and replication process. Methods and ResultsFour novel SaPIs (SaPITokyo12413, SaPITokyo11212, SaPITokyo12571 and SaPITokyo12381) were determined using the SaPI scanning method. These SaPIs were composed of mosaic structures containing reported sequences. Four strains harbouring novel SaPIs produced significant amounts of SEs to cause staphylococcal food poisoning (SFP). With focus on the interaction between the replication initiator protein (Rep) and the replication origin (ori sites) that are proposed to be important for the replication of SaPIs, each Rep was prepared and their two functions were confirmed: binding activity to ori sites and helicase activity. These activities were present in the Reps of SaPITokyo11212, SaPITokyo12571 and SaPITokyo12381, but were both absent in the Rep of SaPITokyo12413. ConclusionsAll four novel SaPIs could give sufficient toxicity to Staph.aureus to cause SFP. However, SaPITokyo12413 may be restricted in its replication capacity, suggesting that it lacks transfer ability unlike the other SaPIs. Significance and Impact of the StudyThis is the first report to identify four novel SE-encoding SaPIs and to examine their toxicity and replication capacity. Because SaPIs deeply participate in SE acquisition, it is important to elucidate their characteristics for understanding Staph.aureus virulence and speculating regarding its evolution as a pathogen.
  • Noriko Ido, Kaori Iwabuchi, Yusuke Sato'o, Yasuo Sato, Masaru Sugawara, Gakuji Yaegashi, Masaru Konno, Masato Akiba, Kiyoshi Tanaka, Katsuhiko Omoe, Ikuo Uchida
    JOURNAL OF VETERINARY MEDICAL SCIENCE 77 (5) 609 - 613 0916-7250 2015/05 [Refereed][Not invited]
     
    Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson's diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.
  • Yasunori Suzuki, Katsuhiko Omoe, Dong-Liang Hu, Yusuke Sato'o, Hisaya K. Ono, Chie Monma, Teruyoshi Arai, Noriko Konishi, Rei Kato, Akihiko Hirai, Akiko Nakama, Akemi Kai, Yoichi Kamata
    MICROBIOLOGY AND IMMUNOLOGY 58 (10) 570 - 580 0385-5600 2014/10 [Refereed][Not invited]
     
    Staphylococcal food poisoning (SFP), one of the commonest food-borne diseases, results from the ingestion of one or more staphylococcal enterotoxins (SEs) produced in foods by Staphylococcus aureus. In the present study, 203 S. aureus strains originating from 83 outbreaks that had occurred in Tokyo were examined for their coagulase type and genotype of SEs to analyze their molecular epidemiological characteristics. The representative subsets of the 83 S. aureus isolates were analyzed by multilocus sequence typing (MLST) and S. aureus pathogenicity island (SaPI) scanning. The isolates were integrated into eight specific clonal complexes (CC) s; CC81, CC8, CC6, CC5, CC508, CC59, CC20 and CC30. The profiles of the coagulase type, SE/SEl genotype and the suspected type of enterotoxin-encoding mobile genetic element (MGE) indicated a correlation with each CC. SaPI scanning showed fixed regularity between the distributions of genomic islands, including SaPIs, and the phylogenetic lineage based on MLST. These results indicate that the S. aureus isolates, which classified into eight CCs, have distinguishable properties concerning specific coagulase type, enterotoxin genotype and MGE type. Strains of S. aureus harboring these particular elements possess the potential to cause SFP.
  • Yusuke Sato'o, Katsuhiko Omoe, Ikunori Naito, Hisaya K. Ono, Akio Nakane, Motoyuki Sugai, Norio Yamagishi, Dong-Liang Hu
    JOURNAL OF CLINICAL MICROBIOLOGY 52 (7) 2637 - 2640 0095-1137 2014/07 [Refereed][Not invited]
     
    Molecular characterization of isolates from staphylococcal food poisoning (SFP) outbreaks in Japan showed that the dominant lineage causing SFP outbreaks is clonal complex 81 (CC81), a single-locus variant of sequence type 1, coagulase type VII, positive for sea and/or seb, and positive for seh. Among various CC lineages producing staphylococcal enterotoxin A, CC81 showed the highest toxin productivity.
  • Naohiro Nishikawa, Katsuhiko Omoe, Kenji Murakami, Yusuke Sato'o, Takekazu Sawa, Yoshihiro Hagihara, Nobuhito Yoshihara, Hiroaki Okawai, Toshirou Iyama, Masahiro Mizuno, Shinya Tsukamoto
    International Journal of Nanomanufacturing 10 (1-2) 185 - 200 1746-9392 2014 [Refereed][Not invited]
     
    In manufacturing, machining fluid as cutting oil or grinding fluid is used for machining. This fluid contains several chemicals such as oil, surface active agent, extreme pressure agent (chlorine, phosphorus, sulphur, etc.), rust preventive agent, antiseptic agent, germicide and so on for improvement machining performance. However, it is not good for human body and environment. It is afraid that workers health hazard is occurred by fluid mist absorption in breath and splash contacting. In addition, waste fluid needs disposal treatment (incineration or coagulative precipitation and landfill, etc.) that is high cost and heavy environmental load. Therefore, environmental friendly and harmless machining is proposed for nanomanufacturing and green manufacturing. The electric rust preventive machining method system is developed in this investigation. This method system use only water (tap water, under ground water, industrial water) as machining fluid. Water only machining lead to greatly decreasing of waste fluid treatment and petroleum oil resources saving. In addition, complete removal of bacteria (Legionella pneumophila) for safe machining water by improvement water recycle system installed reverse osmosis membrane and ultraviolet radiation unit with nonusing chemicals is evaluated by biological cultivation assay. And artificially contaminated tank water bacteria amount tendency is estimated. © 2014 Inderscience Enterprises Ltd.
  • Naohiro Nishikawa, Katsuhiko Omoe, Kenji Murakami, Yusuke Sato'o, Takekazu Sawa, Yoshihiro Hagihara, Nobuhito Yoshihara, Hiroaki Okawai, Toshirou Iyama, Masahiro Mizuno, Shinya Tsukamoto
    INTERNATIONAL JOURNAL OF PRECISION ENGINEERING AND MANUFACTURING 14 (6) 897 - 902 2234-7593 2013/06 [Refereed][Not invited]
     
    It is great concern that is environmental load in manufacturing In machining site, metal working fluid (coolant) such as cutting oil, grinding fluid is used. It contains several chemicals that are oil, surface active agent, extreme pressure additive, antirust agent, antifoaming agent, preservative, biocide etc.. It is thought that it is not good for environment and human body. Machining fluid mist and splash contained several chemicals that are cause of danger for worker's health while machining Furthermore, time elapses, fluid is rotten by bacteria. Bad smell and degradation of machining performance occurs. Therefore, after using machining fluid, waste fluid is disposed. Incineration or coagulative precipitation and landfill etc. are necessary. It arise great disposal cost and environmental load as discharging of huge amount green house gas (CO2 etc). Then, machining fluid decreasing or non-using is demanded in industry. So, in this investigation, new environmental machining method: the electric rust preventive machining method system that uses only water as machining fluid has been developed In this paper water purification recycle system in water machining system development is mentioned Therefore, complete removal of bacteria (Enterobacter aerogenes) without using chemicals such as biocide is examined for corruption, malodor prevention and safe machining water.
  • Yusuke Sato'o, Katsuhiko Omoe, Hisaya K. Ono, Akio Nakane, Dong-Liang Hu
    MICROBIOLOGY AND IMMUNOLOGY 57 (2) 91 - 99 0385-5600 2013/02 [Refereed][Not invited]
     
    Staphylococcus aureus pathogenicity islands (SaPIs) form a growing family of mobile genetic elements (MGEs) in Staphylococci. Horizontal genetic transfer by MGEs plays an important role in the evolution of S. aureus. Several SaPIs carry staphylococcal enterotoxin and SE-like toxin genes. To comprehensively investigate the diversity of SaPIs, a series of primers corresponding to sequences flanking six SaPI insertion sites in S. aureus genome were designed and a long and accurate (LA)-PCR analysis method established. LA-PCR products of 1317kbp were observed in strains with seb, selk or selq genes. Restriction fragment length polymorphism (RFLP) analysis showed that the products have different RFLP characteristics than do previously described SaPIs; they were therefore predicted to include new SaPIs. Nucleotide sequencing analysis revealed seven novel SaPIs: seb-harboring SaPIivm10, SaPishikawa11, SaPIivm60, SaPIno10 and SaPIhirosaki4, selk and selq-harboring SaPIj11 and non-superantigen-harboring SaPIhhms2. These SaPIs have mosaic structures containing components of known SaPIs and other unknown genes. Strains carrying different SaPIs were found to have significantly different production of superantigen toxins. The present results show that the LA-PCR approach can comprehensively identify SaPI diversity and is useful for investigating the evolution of S. aureus pathogenicity.

Books etc

  • 医学と薬学
    佐藤祐介, 菅井基行 (Contributorブドウ球菌)
    自然科学社 2018/07 9784909260055 753-768
  • 分子疫学的手法を用いた日本の高食中毒原性黄色ブドウ球菌の解析
    佐藤祐介 (Contributor)
    岩獣会報 2015
  • 感染症内科
    久恒順三, 達川伸行, 佐藤祐介, 加藤文紀, 鹿山鎮男, 菅井基行 (Contributor黄色ブドウ球菌)
    2013

Conference Activities & Talks

  • クロストーク法によるKlebsiella pneumoniaeに対する広感染宿主域ファージの分離  [Not invited]
    佐藤祐介, 氣駕恒太朗, 渡邊真弥, anit Boonsiri, 李峰宇, 相羽由詞, 李俊傑, Azam Haeruman, 笹原鉄平, 崔龍洙
    日本ファージセラピー研究会 第1回研究集会  2021/11
  • Generation of phagemid-based CRISPR-Cas13 antimicrobials against MRSA
    李 峰宇, 氣駕 恒太朗, in-Ee Tan, 渡邊 真弥, 佐藤 祐介, 相羽 由詞, Kanate Thitiananpakorn, 瀧雄介, 笹原鉄平, 崔龍洙
    第94回日本細菌学会総会  2021/03
  • 毒素性ショック症候群を発症した黄色ブドウ球菌の毒素産生制御機構の解明
    瀧 雄介, 渡邊 真弥, 佐藤 祐介, 李 峰宇, Kanate Thitiananpakorn, XinEe Tan, 相羽 由詞, 氣駕 恒太朗, 笹原 鉄平, 崔 龍洙
    第94回日本細菌学会総会  2021/03
  • 毒素性ショック症候群を発症した黄色ブドウ球菌の毒素産生制御機構の解明
    瀧 雄介, 渡邊 真弥, 佐藤 祐介, 李 峰宇, Kanate Thitiananpakorn, Tanit Boonsiri, 相羽 由詞, 氣駕 恒太朗, 笹原 鉄平, 崔 龍洙
    第93回日本細菌学会総会  2020/02
  • MRSA におけるダプトマイシンとバンコマシンの交差耐性に関わるmprF 変異の役割
    Kanate Thitiananpakorn, 相羽 由詞, 渡邊 真弥, 佐藤 祐介, 氣駕 恒太朗, Tanit Boonsiri, XinEe Tan, Feng-Yu Li, 崔 龍洙
    第93回日本細菌学会総会  2020/02
  • CRISPR-Cas13a 搭載ファージを用いた細菌ゲノム変異の検出
    相羽 由詞, Kanate Thitiananpakorn, 氣駕 恒太朗, 渡邊 真弥, 佐藤 祐介, XinEe Tan, Tanit Boonsiri, 李 峰宇, 崔 龍洙
    2020/02
  • Oxacillin 感性mecA 陽性黄色ブドウ球菌のβ ラクタム薬in vitro高度耐性化機構の解析
    渡邊 真弥, Tanit Boonsiri, Kanate Thitiananpakorn, XinEe Tan, 相羽由詞, 佐藤 祐介, 氣駕 恒太朗, 瀧 雄介, 笹原 鉄平, 崔 龍洙
    第93回日本細菌学会総会  2020/02
  • Staphylococcus capitis におけるグリコペプチド系抗菌薬感受性の乖離機構の解明
    佐藤 祐介, 鐘司 光貴, 渡邊 真弥, 相羽 由詞, 氣駕 恒太朗, 菊池 賢, 平松 啓一, 崔 龍洙
    第93回日本細菌学会総会  2020/02
  • Generation of Bactericidal Chimeric Phages Using SaPI Element of Staphylococcus aureus 黄色ブドウ球菌の SaPI システムを利用した殺菌キメラファージの合成  [Not invited]
    Xin-Ee Tan, Kotaro Kiga, Shinya Watanabe, Yusuke Sato’o, Feng-Yu Li, Aa Haeruman Azam, Víctor Rodrigo, Ibarra Chávez, José R Penadés, Longzhu Cui
    第64回 日本ブドウ球菌研究会  2019/08
  • TCR sequencing facilitates the characterization of human T-cells activation manner following staphylococcal superantigen stimulation  [Invited]
    Fatkhanuddin Aziz, Junzo Hisatsune, Junko Kajimura, Liansheng Yu, Yusuke Sato’o, Hisaya K. Ono, Akio Nakane, Mika Yamaoka, Yoichiro Kusunoki, Hitoshi Komatsuzawa, Hiroki Ohge, Motoyuki Sugai
    第64回日本ブドウ球菌研究会  2019/08
  • ブドウ球菌食中毒の誤謬  [Invited]
    佐藤 祐介, 菅井 基行.
    第64回 日本ブドウ球菌研究会  2019/08
  • 殺菌キメラファージの開発―レプトトリキア科細菌由来の CRISPR-Cas13 システムの機能解析
    Bintao Cui, 渡邊 真弥, 氣駕 恒太朗, 相羽 由詞, 河内 護之, Tanit Boonsiri, Kanate Thitiananpakorn, 佐藤 祐介, Xin Ee Tan, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • Mechanisms of cross-resistant to daptomycin and vancomycin in MRSA  [Not invited]
    Kanate Thitiananpakorn, 相羽由詞, Xin Ee Tan, 渡邊真 弥, 氣駕恒太朗, 佐藤祐介, 河内護之, Tanit Boonsiri, Bintao Cui, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 黄色ブドウ球菌の SaPI-phageシステムを利用した殺菌キメラファージの合成  [Not invited]
    Xin Ee Tan, 氣駕 恒太朗, 渡邊 真弥, 佐藤 祐介, 相羽 由詞, 河内 護之, Kanate Thitiananpakorn, Víctor Rodrigo, Ibarra Chávez,José, R, Penadés, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • Genetic Analysis of Highly β-lactam-resistant Mutants Generated from OS-MRSA  [Not invited]
    Tanit Boonsiri, 渡邊 真弥, Kanate Thitiananpakorn, 佐藤 祐介, 相羽 由詞, 氣駕恒太朗, 笹原 鉄平, 李 峰宇, Xin Ee Tan, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 殺菌キメラファージの開発(6)―大腸菌に広く感染するファージの分離・同定  [Not invited]
    李 峰宇, 氣駕 恒太朗, 渡邊 真弥, 佐藤 祐介, 相羽 由詞, 河内 護之, Xin Ee Tan, Tanit Boonsiri, Kanate Thitiananpakorn, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 殺菌キメラファージの開発(8)―抗菌ファージの開発に資する耐性菌の収集とMRSAファージの分離と同定  [Not invited]
    相羽 由詞, Xin Ee Tan, Kanate Thitiananpakorn, 渡邊 真弥, 氣駕 恒太朗, 佐藤 祐介, Tanit Boonsiri, 李 峰宇, 河内 護之, 崔 龍洙
    23. 第92回日本細菌学会総会  2019/04
  • 殺菌キメラファージの開発(4)―酵母を利用したキメラファージ合成技術の開発  [Not invited]
    河内 護之, 氣駕 恒太朗, 李 峰宇, Tanit Boonsiri, Xin Ee Tan, 佐藤 祐介, 相羽 由詞, Kanate Thitiananpakorn, 渡邊 真弥, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 殺菌キメラファージの開発(1)― 狙った細菌を選択的に殺菌する殺菌技術の開発  [Not invited]
    氣駕 恒太朗, 李 峰宇, Xin Ee Tan, 佐藤 祐介, 渡邊 真弥, 相羽 由詞, íctor Rodrigo, Ibarra Chávez,José, R, Penadés, 鈴木 仁人, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 毒素性ショック症候群を発症した黄色ブドウ球菌の毒素産生制御機構の解明  [Not invited]
    瀧 雄介, 渡邊 真弥, 佐藤 祐介, 李 峰宇, Thitiananpakorn Kanate, Boonsiri Tanit, 相羽 由詞, 氣駕 恒太朗, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 離床分離由来 Staphylococcus argenteusが産生する Staphylococcal Enterotoxin Y の性状解析  [Not invited]
    Fatkhanuddin Aziz, 久恒 順三, 小野 久弥, 梶村 順子, 楠 洋一郎, 佐藤 祐介, 于 連升, 菅井 基行
    第92回日本細菌学会総会  2019/04
  • 緑膿菌に広く感染するファージの分離・同
    渡邊 真弥, 李 峰宇, 氣駕 恒太朗, 相羽 由詞, 佐藤 祐介, Xin Ee Tan, 河内 護之, Tanit Boonsiri,Thitiananpakorn, Kanate,崔 龍洙
    第92回日本細菌学会総会  2019/04
  • 殺菌キメラファージの開発(7)―ファージによる細菌感染症治療モデルの確立  [Not invited]
    佐藤 祐介, 李 峰宇, 氣駕 恒太朗, 渡邊 真弥, 相羽 由詞, 河内 護之, Xin Ee Tan, Tanit Boonsiri, Kanate Thitiananpakorn, 崔 龍洙
    第92回日本細菌学会総会  2019/04
  • DEVELOPMENT OF PHAGE GENOME ASSEMBLY SYSTEM USING YEAST
    Moriyuki Kawauchi, Kotaro Kiga, Feng-Yu Li, Tanit Boonsiri, Xin-Ee Tan, Yusuke Sato’o, Yoshifumi Aiba, Kanate Thitiananpakorn, Shinya Watanabe, Longzhu Cui
    Pacific Basin Consortium International Conference  2019
  • Staphylococcus capitisにおけるバンコマシン/テイコプラニン耐性の乖離機構の解明  [Not invited]
    佐藤 祐介、鐘司 光貴、渡邊 真弥、相羽 由詞、気駕 恒太朗、菊池 賢、平松 啓一、崔龍洙
    第63回 日本ブドウ球菌研究会  2018/09
  • walK mutation is responsible for the reverse cross-resistance of Staphylococcus capitis against vancomycin and teicoplanin  [Not invited]
    Yusuke Sato’o, Mitsutaka Shoji, Shinya Watanabe, Yoshifumi Aiba, Kotaro Kiga, Ken Kikuchi, Keiichi Hiramatsu, Longzhu Cui
    18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2018/08
  • GENETIC EVALUATION OF HIGHLY ß-LACTAM-RESISTANT MUTANTS OF OXACILLIN-SUSCEPTIBLE MECA POSITIVE STAPHYLOCOCCUS AUREUS  [Not invited]
    Tanit Boonsiri, Shinya Watanabe, Kanate Thitiananpakorn, Sato'o Yusuke, Aiba Yoshifumi, Kiga Kotaro, Sasahara Teppei, Li Feng-Yu, Cui Longzhu
    18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2018/08
  • On genetic diversity of β-lactam-resistant population of MRSA  [Not invited]
    Yoshifumi Aiba, Shinya Watanabe, Yusuke Sato’o, Kotaro Kiga, Teppei Sasahara, Kanate Thitiananpakorn, Tanit Boonsiri, Feng-Yu Li, Longzhu Cui
    18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2018/08
  • GENETIC MECHANISMS LINKED TO DAPTOMYCIN AND VANCOMYCIN CROSS-RESISTANCE OF MRSA  [Not invited]
    Kanate Thitiananpakorn, Yoshifumi Aiba, Shinya Watanabe, Yusuke Sato’o, Kotaro Kiga, Teppei Sasahara, Tanit Boonsiri, Longzhu Cui
    18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2018/08
  • COMPLETE GENOME SEQUENCES OF STAPHYLOCOCCUS CAPRAE STRAINS ISOLATED FROM HUMAN AND COMPARATIVE GENOMICS OF S. EPIDERMIDIS, S. CAPITIS AND S. CAPRAE  [Not invited]
    Shinya Watanabe, Yoshifumi Aiba, Xin-Ee Tan, Fengyu Li, Tanit Boonsiri, Kanate Thitiananpakorn, Bintao Cui, Yusuke Sato'o, Kotaro Kiga, Teppei Sasahara, Longzhu Cui
    18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2018/08
  • On the genetic alterations conferring β-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus.  [Not invited]
    Boonsiri Tanit, Shinya Watanabe, Thitiananpakorn Kanate, Yusuke Sato’o, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, XinEe Tan, Bintao Cui, Feng-Yu Li, Longzhu Cui
    第63回日本ブドウ球菌研究会  2018/08
  • MRSAのβラクタム系抗菌薬耐性における遺伝学的多様性  [Not invited]
    相羽 由詞, Kanate Thitiananpakorn, 渡邊 真弥, Tanit Boonsiri, 佐藤 祐介, 氣駕 恒太朗, 李 峰宇, 笹原 鉄平, 崔 龍洙
    第63回日本ブドウ球菌研究会  2018/08
  • Oxacillin感性を示す mecA 陽性黄色ブドウ球菌のβ-ラクタム薬感性化機構の解明  [Not invited]
    渡邊 真弥, Boonsiri Tanit,Thitiananpakorn Kanate, 相羽 由詞, 佐藤 祐介, 氣駕 恒太朗, 笹原 鉄平, 崔 龍洙
    第91回日本細菌学会総会 (2018)  2018/03
  • Identification of mutations associated with cross-resistance to daptomycin and vancomycin in MRSA  [Not invited]
    Kanate Thitiananpakorn, 相羽 由詞, 渡邊 真弥, 佐藤 祐介, 氣駕 恒太朗, 笹原 鉄平, Tanit Boonsiri, 李 峰宇, 崔 龍洙
    第91回日本細菌学会総会  2018/03
  • MRSAのβ-ラクタム系抗菌薬耐性における遺伝学的多様性の解明  [Not invited]
    相羽 由詞, 渡邊 真弥, 佐藤 祐介, 氣駕 恒太朗, Kanate Thitiananpakorn, Tanit Boonsiri, 李 峰宇, 笹原 鉄平, 崔 龍洙
    第91回日本細菌学会総会  2018/03
  • Genetic mechanisms of high β-lactam resistance in Methicillin- resistant Staphylococcus aureus  [Not invited]
    Boonsiri Tanit, 渡邊 真弥, Thitiananpakorn Kanate, 佐藤 祐介, 相羽 由詞, 氣駕 恒太朗, 笹原 鉄平, 李 峰宇, 崔 龍洙
    第91回日本細菌学会総会  2018/03
  • ブドウ球菌属細菌における高効率エレクトロポレーション法の確立  [Not invited]
    佐藤 祐介,相羽 由詞,氣駕 恒太朗,渡邊 真弥,笹原 鉄平,早川 靖彦,崔 龍洙
    第91回日本細菌学会総会  2018/03
  • 酵母を利用したファージ合成技術の検討  [Not invited]
    河内 護之, 氣駕 恒太朗, 佐藤 祐介, 相羽 由詞, 笹原 鉄平, 渡邊 真弥, 崔 龍洙
    第101回日本細菌学会関東支部総会  2018
  • A unique staphylococcal enterotoxin SEY: prevalence and it’s characterization  [Not invited]
    Fatkhanuddin Aziz, Junzo Hisatsune, Yu Liansheng, Kanako Masuda, Yusuke Sato’o, Hisaya K. Ono, Junko Kajimura, Yoichiro Kusunoki, Motoyuki Sugai
    第71回日本細菌学会中国・四国支部総会  2018
  • ブドウ球菌食中毒特異的クローンの同定とその解析  [Invited]
    佐藤祐介
    第62回ブドウ球菌研究会  2017/09
  • Identification of gene mutations associated with daptomycin and vancomycin cross-resistance in Staphylococcus aureus  [Not invited]
    Kanate Thitiananpakorn, Yoshifumi Aiba, Shinya Watanabe, Yusuke Sato’o, Tanit Boonsiri, Kotaro Kiga, Feng-Yu Li, Teppei Sasahara, Longzhu Cui
    第62回ブドウ球菌研究会  2017/08
  • Oxacillin感性を示すmecA陽性黄色ブドウ球菌のOxacillin感性化機構の解明  [Not invited]
    渡邊 真弥, Tanit Boonsiri, Kanate Thitiananpakorn, 相羽 由詞, 佐藤 祐介, 氣駕恒太朗, 李 峰宇, 笹原 鉄平, 崔 龍洙
    第62回ブドウ球菌研究会  2017/08
  • Association of walk and murA mutations with vancomycin susceptibility and biofilm in Staphylococcus  [Not invited]
    佐藤 祐介,鐘司 光貴,渡邊 真弥,崔 龍洙.
    第90回日本細菌学会総会  2017/03
  • グリコペプチド耐性 Staphylococcus capitis のゲノム解析  [Not invited]
    佐藤祐介, 鐘司光貴, 渡邊真弥, 崔龍洙
    日本細菌学会 関東支部 インターラボセミナー 2016 in 栃木  2016/10
  • Genomic analysis of Staphylococcus capitis resistant to glycopeptide antibiotics  [Not invited]
    Yusuke Sato’o, Mitsutaka Shoji, Shinya Watanabe, Ken Kikuchi, Keiichi Hiramatsu, Longzhu
    17th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2016/08
  • Genomic analysis of Staphylococcus capitis resistant to glycopeptide antibiotic  [Not invited]
    Yusuke Sato’o, Mitsutaka Shoji, Shinya Watanabe, Ken Kikuchi, Keiichi Hiramatsu, Longzhu
    17th International Symposium on Staphylococci and Staphylococcal Infections  2016/08
  • Genome analysis of Staphylococcus capitis resistant to glycopeptide antibiotics  [Not invited]
    Yusuke Sato’o, Mitsutaka Shoji, Shinya Watanabe, Ken Kikuchi, Keiichi Hiramatsu, Longzhu Cui
    第89回日本細菌学会総会  2016/03
  • ブドウ球菌エンテロトキシンを保有する新規 SaPI の同定とその特性  [Not invited]
    鈴木 康規, 久保田 寛顕, 佐藤 祐介, 小野 久弥, 加藤 玲, 甲斐 明美, 平井 昭彦, 貞升 健志, 鎌田 洋一
    第 98 回日本細菌学会関東支部総会  2015/09
  • 黄色ブドウ球菌が産生する新規Staphylococcal_enterotoxinの解析  [Not invited]
    佐藤 祐介、久恒 順三、小野 久弥、胡 東良、中根 明夫、菅井 基行
    第158回日本獣医学会総会  2015/09
  • ブドウ球菌エンテロトキシン H(SEH)の食中毒における重要性  [Not invited]
    佐藤祐介、久恒順三、菅井基行
    第88回日本細菌学会総会  2015/03
  • ブドウ球菌エンテロトキシン関連プラスミドの食中毒事例への関与と変異型 sed 遺伝子の同定
    鈴木康規, 佐藤祐介, 小野久弥, 小林真紀, 松下 秀, 加藤 玲, 小西典子, 門間千絵, 甲斐明美, 平井昭彦, 貞升健志, 鎌田洋一
    第36回日本食品微生物学会学術総会  2015
  • ブドウ球菌エンテロトキシンを保有する新たなStaphylococcus aureus pathogenicity islandsの同定およびその特性解析  [Not invited]
    鈴木康規, 重茂克彦, 佐藤祐介, 小野久弥, 門間千枝, 小西典子, 尾畑浩魅, 小林真紀子, 松下秀, 上原さとみ, 加藤玲, 貞升健志, 甲斐明美, 鎌田洋一
    第157回日本獣医学会総会  2014/09
  • 臨床および食中毒由来黄色ブドウ球菌のスーパー抗原毒素遺伝子とその発現の比較検討  [Not invited]
    樋爪裕美, 岡田玲奈, 岡村雅史, 佐藤祐介, 小野久弥, 菅井基行, 中根明夫, 胡東良
    第157回日本獣医学会総会  2014/09
  • ブドウ球菌エンテロトキシンH(SEH)の発現制御機構と食中毒における重要性  [Not invited]
    佐藤祐介、久恒順三、菅井基行
    第157回日本獣医学会総会  2014/09
  • Identification and Characterization of a Novel Staphylococcal Emetic Toxin Encoded in Staphylococcus aureus Core Genome  [Not invited]
    Identification, Characterization of, a, Novel, Staphylococcal Emetic Toxin, Encoded in Staphylococcus aureus Core Genome
    16th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI)  2014/08
  • 臨床および食中毒由来黄色ブドウ球菌のスーパー抗原毒素遺伝子とその発現の比較検討  [Not invited]
    胡東良, 樋爪裕美, 岡田玲奈, 岡村雅史, 佐藤祐介, 小野久弥, 菅井基行, 中根明夫
    第68回 日本細菌学会東北支部総会  2014/08
  • 黄色ブドウ球菌ゲノム中に存在する新規嘔吐毒の生物活性.  [Not invited]
    小野久弥, 佐藤祐介, 久恒順三, 菅井基行, 重茂克彦, 中根明夫
    第 87 回 日本細菌学会総会  2014/03
  • Molecular epidemiology of S. aureus isolated from food poisoning.  [Not invited]
    佐藤祐介、重茂克彦、小野久弥、中根明夫、菅井基行、胡東良.
    第 87 回 日本細菌学会総会  2014/03
  • 東京都で分離された食中毒事例由来 Staphylococcus aureus の分子疫学的解析  [Not invited]
    鈴木康則, 門間千枝, 荒井輝義, 小西典子, 尾畑浩魅, 上原さとみ, 加藤玲, 平井昭彦, 佐藤祐介, 小野久弥, 品川邦汎, 重茂克彦, 仲真晶子, 甲斐明美
    第155回日本獣医学会  2013/09
  • 黄色ブドウ球菌ゲノム上に存在する新規エンテロトキシンSEYの生物活性  [Not invited]
    小野久弥, 内藤郁慶, 佐藤祐介, 菅井基行, 重茂克彦, 中根明夫
    第67回日本細菌学会東北支部総会  2013/08
  • Staphylococcus aureus pathogenicity islandsの新規解析手法の確立とそれを用いた解析  [Not invited]
    佐藤 祐介、重茂 克彦、小野 久弥、中根 明夫、胡 東良
    第155回日本獣医学会  2013/03
  • 血清型Typhimurium単相変異株としてのサルモネラO4:i:-の性状  [Not invited]
    井戸 徳子, 岩渕 香織, 佐藤 祐介, 李 謙一, 内田 郁夫, 楠本 正博, 岩田 剛敏, 重茂 克彦, 秋庭 正人
    第154回日本獣医学会学術集会  2012/09
  • 黄色ブドウ球菌の分子生物学的解析  [Invited]
    佐藤祐介, 内藤郁慶, 小野久弥, 菅井基行, 久恒順三, 瀬尾宏美, 上原良雄, 胡東良, 中根明夫, 重茂克彦
    第57回 日本ブドウ球菌研究会  2012/09
  • 食品中の新型エンテロトキシンに対する温度影響の評価  [Not invited]
    福間幹大, 佐藤明彦, 長廻ゆりあ, 佐藤祐介, 小野久弥, 胡 東良, 中根明夫, 重茂克彦
    第66回日本細菌学会東北支部総会 (2012)  2012/08
  • Genetic features of Staphylococcus aureus carring Staphylococcal enterotoxin Type H  [Not invited]
    Yusuke Sato, Hisaya Ono, Dong-Liang Hu, Akio Nakane, Katsuhiko Omoe
    第85回日本細菌学会  2012/03
  • DENTIFICATION OF MAJOR CLONAL COMPLEXES OF FOOD POISONING-RELATED STAPHYLOCOCCUS AUREUS IN JAPAN  [Not invited]
    Yusuke Sato'o, Hisaya K. Ono, Mari Shibata, Mikihiro Fukuma, Katsuhiko Omoe
    International Union of Microbiological Societies 2011 congress (2011)  2011/09
  • TEMPERATURE DEPENDENT REGULATION OF ENTEROTOXIN-GENE-CLUSTER-RELATED STAPHYLOCOCCAL ENTEROTOXINS PRODUCTION  [Not invited]
    Akihiko Sato, Yuria Nagasako, Yuki Yamamoto, Yusuke Sato, Hisaya K Ono, Dong-Liang Hu, Akio Nakane, Katsuhiko Omoe
    International Union of Microbiological Societies 2011 congress  2011/09
  • 家畜及びヒトから分離されたSalmonella O4:i:-の分子疫学  [Not invited]
    井戸 徳子, 長山 玲子, 千葉 伸, 岩渕 香織, 佐藤 祐介, 秋庭 正人, 重茂 克彦
    第152回日本獣医学会学術集会  2011/09
  • 食中毒事例分離黄色ブドウ球菌におけるゲノム構成の解明  [Not invited]
    佐藤 祐介、小野 久弥、胡 東良、中根 明夫、重茂 克彦
    第152回日本獣医学会学術集会  2011/09
  • ブドウ球菌エンテロトキシンおよびエンテロトキシン様毒素産生に対する温度の影響  [Not invited]
    佐藤明彦, 長廻ゆりあ, 山本裕紀, 小野久弥, 佐藤祐介, 胡 東良, 中根明夫, 重茂克彦
    第65回 日本細菌学会東北支部総会  2011/08
  • ブドウ球菌エンテロトキシンB関連新規staphylococcus aureus pathogenicity islandsの同定  [Not invited]
    佐藤祐介、小野久弥、胡東良、中根明夫、重茂克彦
    第33回日本分子生物学会  2010/12
  • 黄色ブドウ球菌エンテロトキシンB遺伝子を保有する新規Staphylococcus aureus pathogenicity islandの解析  [Not invited]
    佐藤祐介、小野久弥、胡東良、中根明夫、重茂克彦
    第64回 日本細菌学会東北支部総会  2010/08
  • ブドウ球菌エンテロトキシンB遺伝子を保有する新規staphylococcus aureus pathogenicity islandsの解析  [Not invited]
    佐藤祐介、小野久弥、胡東良、中根明夫、重茂克彦
    第83回日本細菌学会 2010/3横浜  2010/03
  • A unique staphylococcal enterotoxin SEY: prevalence and it’s characterization  [Not invited]
    Fatkhanuddin Aziz, Junzo Hisatsune, Yu Liansheng, Kanako Masuda, Yusuke Sato’o, Hisaya K. Ono, Junko Kajimura, Yoichiro Kusunoki, Motoyuki Sugai
    第63回 日本ブドウ球菌研究会

MISC

Awards & Honors

  • 2021/11 Japanese 􏰂ociety for Phage 􏰅herapy 若手優秀発表賞
     クロストーク法によるKlebsiella pneumoniaeに対する広感染宿主域ファージの分離
  • 2015/03 Japanese society of bacteriology Poster award
     
    受賞者: Yusuke Sato'o
  • 2015/03 日本細菌学会 優秀ポスター賞
     
    受賞者: 佐藤祐介
  • 2014/09 Japanese Society of Veterinary Science encouragement award (subcommittee of public health)
     
    受賞者: Yusuke Sato'o
  • 2014/09 日本獣医学会 公衆衛生分科会奨励賞
     
    受賞者: 佐藤祐介

Research Grants & Projects

  • 細菌叢の正常化と疾患治療を目的としたバクテリオファージ療法の開発
    Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
    Date (from‐to) : 2020/04 -2023/03 
    Author : 佐藤 祐介
  • 細菌のゲノム再編成とそれによる持続感染機構の解明 研究課題
    JSPS:Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2017/04 -2020/03 
    Author : Yusuke Sato'o
  • RNA 標的型 CRISPR/Cas 搭載抗菌バクテリオファージを活用した腸内細菌叢編集技術の構築
    TBRF:東京生化学研究会研究助成
    Date (from‐to) : 2019 -2020 
    Author : Yusuke Sato'o
  • 高食中毒原性黄色ブドウ球菌が産生する新型エンテロトキシンの危害評価
    旗影会:一般助成
    Date (from‐to) : 2016/04 -2017/03 
    Author : 佐藤祐介


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