Researchers Database

yoshimura kotaro

    SurgeryPlasticSurgery Professor
Last Updated :2021/11/23

Researcher Information

Research funding number

  • 60210762

J-Global ID

Research Interests

  • 放射線障害   脂肪幹細胞   keloid   Mechanobiology   培養皮膚   レチノイド   血管新生   Tissue repair/regeneration   Wound healing   虚血   抗加齢   色素   血管   毛髪   脂肪   形成医学   再生医学   hypoxia   angiogenesis   Dermal papilla   Skin   Adipose   Cell therapy   Tissue engineering   Cosmetic medicine   Regeneration medicine   

Research Areas

  • Life sciences / Plastic and reconstructive surgery
  • Life sciences / Cell biology

Academic & Professional Experience

  • 1998/07  The University of TokyoFaculty of Medicine講師
  • 1994/05 - 1995/06  ミシガン大学Visiting Researcher

Education

  •        - 1985  The University of Tokyo  Faculty of Medicine  School of Medicine

Association Memberships

  • 国際脂肪研究治療学会(IFATS)   米国美容外科学会(ASAPS)   JAPAN SOCIETY OF AESTHETIC PLASTIC SURGERY   米国形成外科学会(ASPS)   国際美容外科学会(ISAPS)   International Society of Plastic Regenerative Surgery   Japanese Society of Oncoplastic Breast Surgery   日本抗加齢医学会   PSRC   IPRAS   THE JAPANESE SOCIETY FOR REGENERTIVE MEDICINE(JSRM)   JAPAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGERY   

Published Papers

  • Szu-Hsien Wu, Takako Shirado, Takanobu Mashiko, Jingwei Feng, Rintaro Asahi, Koji Kanayama, Masanori Mori, Daekwan Chi, Ataru Sunaga, Shunji Sarukawa, Kotaro Yoshimura
    Plastic and reconstructive surgery 142 (2) 383 - 391 0032-1052 2018/08 [Refereed][Not invited]
     
    BACKGROUND: Clinical sequelae of irradiation result in tissue devitalization (e.g., ischemia, fibrosis, and atrophy) where wound healing capacity is impaired. Fat-derived products may work to treat such pathology. METHODS: Nonlethal irradiation at various doses (5, 10, and 15 Gy) and frequencies (one to three times on sequential days) was delivered to dorsal skin of nude mice, and subsequent gross and microscopic changes were evaluated for up to 4 weeks. Cutaneous punch wounds were then created to compare wound healing in irradiated and nonirradiated states. Wounds were also locally injected with vehicle, cultured adipose-derived stem cells, centrifuged fat tissue, or micronized cellular adipose matrix, and the therapeutic impact was monitored for up to 15 days. RESULTS: Nude mice given total doses greater than 15 Gy spontaneously developed skin ulcers, and radiation damage was dose-dependent; however, a fractionated irradiation protocol was able to reduce the damage. Histologic assessment revealed dose-dependent dermal fibrosis/thickening and subcutaneous atrophy. Dose-dependent (5 to 15 Gy) impairment of wound healing was also evident. At the highest dosage (15 Gy three times), open wounds persisted on day 15. However, wounds injected with cultured adipose-derived stem cells were nearly healed on day 12, and those treated with injection of centrifuged fat or micronized tissue healed faster than untreated controls (p < 0.05). There was no significant differences between treated groups. CONCLUSIONS: Tissue devitalization by irradiation was dose-dependent, although fractionated protocols helped to reduce it. Adipose-derived stem cells and other fat-derived products harboring adipose-derived stem cells successfully revitalized irradiated tissues and accelerated wound healing.
  • Yoshimura K
    Plastic and reconstructive surgery 142 (1) 122 - 123 0032-1052 2018/07 [Refereed][Not invited]
  • Takanobu Mashiko, Hitomi Takada, Szu-Hsien Wu, Koji Kanayama, Jingwei Feng, Kensuke Tashiro, Rintaro Asahi, Ataru Sunaga, Kazuto Hoshi, Akira Kurisaki, Tsuyoshi Takato, Kotaro Yoshimura
    Journal of tissue engineering and regenerative medicine 12 (5) 1186 - 1194 1932-6254 2018/05 [Refereed][Not invited]
     
    Chronic changes following radiotherapy include alterations in tissue-resident stem cells and vasculatures, which can lead to impaired wound healing. In this study, novel recombinant human collagen peptide (rhCP) scaffolds were evaluated as a biomaterial carrier for cellular regenerative therapy. Human adipose-derived stem cells (hASCs) were successfully cultured on rhCP scaffolds. By hASC culture on rhCP, microarray assay indicated that expression of genes related to cell proliferation and extracellular matrix production was upregulated. Pathway analyses revealed that signaling pathways related to inflammatory suppression and cell growth promotion were activated as well as signaling pathways consistent with some growth factors including vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta, although gene expression of these growth factors was not upregulated. These findings suggest the rhCP scaffold showed similar biological actions to cytokines regulating cell growth and immunity. In subsequent impaired wound healing experiments using a locally irradiated (20 Gray) mouse, wound treatment with rhCP sponges combined with cultured hASCs and human umbilical vein endothelial cells accelerated wound closure compared with wounds treated with rhCP with hASCs alone, rhCP only, and control (dressing alone), with better healing observed according to this order. These results indicating the therapeutic value of rhCP scaffolds as a topical biomaterial dressing and a biocarrier of stem cells and vascular endothelial cells for regenerating therapies. The combination of rhCP and functional cells was suggested to be a potential tool for revitalizing stem cell-depleted conditions such as radiation tissue damage.
  • Chi D, Kamochi H, Sunaga A, Sarukawa S, Uda H, Nakagawa S, Asahi R, Mori M, Yoshimura K
    Plastic and reconstructive surgery. Global open 6 (4) e1730  2018/04 [Refereed][Not invited]
  • Takanobu Mashiko, Szu-Hsien Wu, Koji Kanayama, Rintaro Asahi, Takako Shirado, Masanori Mori, Ataru Sunaga, Shunji Sarukawa, Hirokazu Uda, Kotaro Yoshimura
    Plastic and reconstructive surgery 141 (1) 104 - 115 0032-1052 2018/01 [Refereed][Not invited]
     
    BACKGROUND: Fat grafting frequently requires multiple treatments and thus repeated liposuction to achieve treatment goals. The purpose of this study was to evaluate whether cryopreservation of adipose tissue may facilitate future fat grafting. METHODS: Lipoaspirates were harvested from six women and preserved using two cryopreservation methods: (1) simple cooling to -80°C (cryo-1); or (2) programmed cooling to -196°C (cryo-2). Fresh fat, cryo-1 fat, and cryo-2 fat were analyzed both in vitro and in vivo. RESULTS: Immunohistochemistry of both types of cryopreserved adipose tissue revealed that most adipocytes were necrotic. The cell number and viability of stromal vascular fraction cells were significantly decreased in cryo-1 fat (1.7 × 10 cells, 42.6 percent viable) and cryo-2 fat (2.0 × 10 cells, 55.4 percent viable), compared with fresh fat (3.9 × 10 cells, 90.6 percent viable). Although adipose-derived stem cells were cultured successfully from all fats, functional adipose-derived stem cells from cryopreserved fats were much fewer, with comparable multilineage differentiating capacity. In vivo studies using human fat grafted into immunocompromised mice revealed that, 3 months after transplantation, all of the cryopreserved fats maintained their volume to some extent; however, the cryopreserved fats were mostly filled with dead tissue and produced significantly lower engraftment scores than fresh fat. CONCLUSIONS: Most adipocytes were killed in the process of cryopreservation and thawing. Adipose-derived stem cells were isolated from cryopreserved fat, but the number of functional adipose-derived stem cells was very limited in both cryopreservation methods. After grafting, cryopreserved fat was retained as dead and fibrous tissue, suggesting a risk of clinical complications such as oil cysts.
  • Hirokazu Uda, Hideaki Kamochi, Syunji Sarukawa, Ataru Sunaga, Yasushi Sugawara, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 140 (6) 1101 - 1109 0032-1052 2017/12 [Refereed][Not invited]
     
    Background: The deep inferior epigastric perforator (DIEP) flap, which is a modification of the muscle-sparing free transverse rectus abdominis musculocutaneous (TRAM) flap, is being more frequently used in an effort to reduce postoperative abdominal morbidity. However, there is no consensus as to which of these flaps is superior. The authors aimed to compare quantitative measurements of abdominal function obtained with an isokinetic dynamometer after DIEP and muscle-sparing free TRAM flap elevation. Methods: Patients who underwent unilateral single-pedicled DIEP (n = 42) or muscle-sparing free TRAM flap (n = 36) breast reconstruction performed by a single surgeon were included in this study. Preoperative and postoperative trunk flexion parameters were measured prospectively using an isokinetic dynamometer in all patients. The occurrence of postoperative pain, stiffness, and bulging along with patient activity level were also investigated. Results: At 3 months postoperatively, abdominal functions were decreased in both groups, with a larger decline in the muscle-sparing free TRAM flap group. However, at 6 months postoperatively, abdominal muscle function recovered to preoperative levels in both groups. These findings were consistent with the absence of a statistically significant difference in patient postoperative abdominal pain and stiffness, activity level, and the incidence of bulging between the two groups at 6 months postoperatively. Conclusion: From these results, we propose that the surgeon can select the muscle-sparing free TRAM flap, without hesitation or concern regarding abdominal morbidity, when a thick and reliable perforator does not exist and multiple thin perforators must be incorporated. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, III.
  • Koji Kanayama, Takanobu Mashiko, Rintaro Asahi, Ataru Sunaga, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 140 (6) 831E - 832E 0032-1052 2017/12 [Refereed][Not invited]
  • Hideaki Kamochi, Ataru Sunaga, Daekwan Chi, Rintaro Asahi, Shiho Nakagawa, Masanori Mori, Hirokazu Uda, Shunji Sarukawa, Yasushi Sugawara, Kotaro Yoshimura
    JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY 45 (11) 1842 - 1845 1010-5182 2017/11 [Refereed][Not invited]
     
    Background: Although the charting of normal intracranial volume (ICV) is fundamental for managing craniosynostosis, Asian norms in this regard are unknown. The purpose of this study was to establish a growth curve for ICVs in a large series of normal Asian children, providing reference values to guide corrective surgery. Methods: A total of 124 normal children (male, 63; female, 61) and 41 children diagnosed with craniosynostoses were analyzed. Patients aged 0-8 years presenting to the emergency room and subjected to computed tomography (CT) for head trauma served as the reference cohort. Axial CT head scan data were obtained from radiographic archives at Jichi Medical University. Imaging was done on a Siemens CT scanner (5-mm slice thickness), using a DICOM viewer to measure ICVs. Results: ICVs were plotted against age, and best-fit logarithmic curves for normal subjects were generated, without and with gender stratification. Male and female growth curves were similar in shape but diverged past the age of 1 year (male > female). ICVs of patients with craniosynostoses were plotted to male and female growth curves by disease subset, revealing the following: sagittal synostosis, near normal (or marginally larger); metopic synostosis, below normal; other non-syndromic synostoses (unilateral, bilateral, and lambdoidal) and Crouzon syndrome, near normal; Apert syndrome, above normal; and Pfeiffer syndrome, variable. Conclusion: ICVs of early childhood were investigated in Asian subjects, creating growth curves that set criteria for timing, planning and goalsetting in surgical correction of craniosynostosis. (C) 2017 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
  • Keishi Kawasaki, Dan Takeuchi, Takaaki Kaneko, Seiki Miura, Junichiro Kamiya, Yoji Miyahara, Kotaro Yoshimura, Akira Ogata
    Japanese Journal of Cancer and Chemotherapy 44 (11) 1017 - 1020 0385-0684 2017/11 [Refereed][Not invited]
     
    We report a 72-year-old woman who was initially diagnosed with locally advanced gastric cancer with involvement of the esophagus and pancreas. She received 3 courses of neoadjuvant chemotherapy (NAC) with docetaxel, cisplatin, and 5- fluorouracil (the DCF regimen) and achieved an excellent response. She underwent total gastrectomy with distal pancreatectomy, splenectomy, and D2 lymphadenectomy. Histological examination confirmed a pathological complete response. NAC chemotherapy can down stage/down size the disease and allow some patients to undergo curative radical surgery.
  • Yoko Katsuragi Tomioka, Hirokazu Uda, Kotaro Yoshimura, Ataru Sunaga, Hideaki Kamochi, Yasushi Sugawara
    JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY 70 (10) 1391 - 1396 1748-6815 2017/10 [Refereed][Not invited]
     
    Background: The proximal ends of internal mammary (IM) vessels are now the most common recipient vessels for breast reconstruction. On the other hand, bilateral deep inferior epigastric artery perforator (DIEP) flaps are often needed according to the territory and the volume required for reconstruction. The usefulness of retrograde IM vessels as second recipients has recently been reported, but there are very few quantitative studies on the hydrodynamics of the retrograde IM vessels. Because the flow is dependent on the pressure differential, the blood pressures of the antegrade IM artery (AIMA), antegrade IM vein (AIMV), retrograde IM artery (RIMA), retrograde IM vein (RIMV), and recirculated intraflap vein (FV) were investigated to solve this question and to confirm the reliability and usefulness of the retrograde IM vessels. Methods: Ten free flap breast reconstructions were included in this study. The IM vessels were exposed, and the pressures were measured. After recirculation, the FV pressures were measured when the flap was not ischemic or congestive. Systemic blood pressure was also recorded during the whole measurement period. Results: The AIMA and RIMA pressures were 70.4 +/- 8.2 mmHg and 54.0 +/- 8.6 mmHg (p = 0.000003), respectively, while the systemic pressure was 65.1 +/- 10.0 mmHg. The AIMV pressure was always smaller than the RIMV pressure; the mean AIMV pressure was 5.3 +/- 1.6 mmHg. In addition, the FV pressure was greater (p = 0.03) than the RIMV pressure (17.7 +/- 9.9 mmHg), while the RIMV pressure was 8.7 +/- 2.0 mmHg. Conclusions: Both the RIMA and RIMV are useful and reliable as second recipients for bipedicled free flap transfers. This is a great benefit because it would provide two recipients in one surgical site and would be especially useful in thin patients or patients with previous abdominal scars requiring double pedicled DIEP flaps. Level of evidence: Therapeutic Study, Level IV. (C) 2017 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
  • Shunji Sarukawa, Hideaki Kamochi, Tadahide Noguchi, Ataru Sunaga, Hirokazu Uda, Yoshiyuki Mori, Hiroshi Nishino, Kotaro Yoshimura
    JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY 45 (9) 1573 - 1577 1010-5182 2017/09 [Refereed][Not invited]
     
    Anteromedial maxillectomy is typically performed in conjunction with low-dose radiotherapy and intraarterial chemotherapy. In doing so, the extent of surgical defects is reduced. However, nasal deviation and oral incompetence may ensue, due to cicatricial contracture of wounds, and may be distressing to these patients. Herein, we report a series of eight free perforator flap procedures (anterolateral thigh [ALT] flap, 6; thoracodorsal artery perforator [TAP] flap, 2) used to correct such deformities. The TAP flap was combined with scapular tip [ST] osseous flap in patients with added zygomatic prominence defects. Three adipocutaneous parts developed from each perforator flap were applied as follows: two to reconstruct nasal lining and oral vestibule, and one to augment cheek volume. All aesthetic results proved satisfactory, although orbital dystopia and contracture of mimic muscles were not resolved completely. These secondary interventions are suitable for sequelae of simple anteromedial maxillectomy. Immediate reconstruction should be considered if orbital floor and mimic muscles are involved. (C) 2017 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
  • Yoko Tomioka, Shintaro Enomoto, Jian Gu, Akiko Kaneko, Itsuro Saito, Yusuke Inoue, Taeseong Woo, Isao Koshima, Kotaro Yoshimura, Takao Someya, Masaki Sekino
    SCIENTIFIC REPORTS 7 (1) 9643  2045-2322 2017/08 [Refereed][Not invited]
     
    Compromised circulation is a potential complication during the postoperative period following tissue transplantation. The use of a monitoring device allows physicians to detect compromised circulation immediately. Such monitoring devices need to be continuously usable, wearable, and area-detectable. However, existing devices fail to satisfy all of these requirements simultaneously. We developed a wearable, multipoint pulse wave-monitoring device. An array of reflective optical sensors implemented on a thin film substrate was used as a lightweight and flexible probe. As a model of tissue transplantation, an inguinal flap in a Wistar rat was dissected and freed from all subcutaneous tissue. By ligating the artery or vein, ischemia or congestion was induced in the tissue. In a human study, ischemia or congestion was induced in the palm by pressing the feeding artery or cutaneous vein, respectively. The amplitude of the pulse wave was evaluated using the power spectrum of Fourier transformed signals. Pulse wave amplitude significantly decreased under compromised circulation in both animal and human models. Moreover, we accomplished 1 week of continuous wireless monitoring in healthy subjects. These results demonstrated the potential utility of the developed device in postoperative blood-flow monitoring to improve the rescue rate of transplanted tissue.
  • Takanobu Mashiko, Szu-Hsien Wu, Jingwei Feng, Koji Kanayama, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 139 (6) 1370E - 1371E 0032-1052 2017/06 [Refereed][Not invited]
  • Masanori Mori, Hayato Nagashima, Satoshi Akazawa, Noriko Saegusa, Yuichi Ichikawa, Kei Iida, Kotaro Yoshimura, Masahiro Nakagawa
    Plastic and Reconstructive Surgery - Global Open 5 (6) e1366  2169-7574 2017/06 [Refereed][Not invited]
     
    Takotsubo cardiomyopathy (TCM) is a form of transient heart failure that clinically mimics acute coronary syndrome and is characterized by left ventricular wall motion abnormalities. The pathophysiology of TCM is not well established. TCM is often preceded by emotional or physical stress and may occur after surgery. We present 3 cases of TCM occurring after head and neck reconstructive surgery. Echocardiography plays a central role in the diagnosis of TCM. Left ventricular wall motion abnormalities extend beyond the territory of a single coronary artery. Coronary angiography and cardiac computed tomography can demonstrate the absence of coronary atherosclerosis and are useful for confirming the diagnosis of TCM. Particularly after reconstructive surgery, it is necessary to carefully monitor fluid replacement to avoid dehydration, which may compromise flap blood flow, although congestive heart failure is the most common complication of TCM. It is important to encourage ambulation as soon as possible, while considering the degree of cardiac impairment.
  • Jingwei Feng, Kazuhide Mineda, Szu-Hsien Wu, Takanobu Mashiko, Kentaro Doi, Shinichiro Kuno, Kahori Kinoshita, Koji Kanayama, Rintaro Asahi, Ataru Sunaga, Kotaro Yoshimura
    SCIENTIFIC REPORTS 7 (1) 1548  2045-2322 2017/05 [Refereed][Not invited]
     
    For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D culture vessel. Spheroids of cells formed after 12 h. We found that 6 x 10(6) ASCs/ml in 3% HA gel achieved the highest spheroid density with appropriate spheroid sizes (20-100 mu m). Immunocytology revealed that the stem cell markers, NANOG, OCT3/4, SOX-2, and SSEA-3 were up-regulated in the ASC spheroids compared with those in nonadherent-dish spheroids or in monolayer cultured ASCs. In delayed wound healing mice models, diabetic ulcers treated with ASC spheroids demonstrated faster wound epithelialization with thicker dermis than those treated with vehicle alone or monolayer cultured ASCs. In irradiated skin ulcers in immunodeficient mice, ASC spheroids exhibited faster healing and outstanding angiogenic potential partly by direct differentiation into alpha-SMA+ pericytes. Our method of 3D in-syringe HA gel culture produced clinically relevant amounts of ready-to-inject human ASC microspheroids that exhibited superior stemness in vitro and therapeutic efficacy in pathological wound repair in vivo.
  • Hideaki Kamochi, Shunji Sarukawa, Hirokazu Uda, Hiroshi Nishino, Kotaro Yoshimura
    JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY 75 (2) 439.e1 - 439.e6 0278-2391 2017/02 [Refereed][Not invited]
     
    Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latissimus dorsi musculocutaneous and scapular angle osseous free flap (LD-SA flap). The LD-SA flap has substantial advantages, such as providing structural support to the malar prominence, filling the large soft tissue defect, and preventing postoperative drooping of the cheek. The surgical technique is relatively straightforward, requires a shorter operative time, and produces less blood loss compared with other reconstruction approaches. The LD-SA flap is a useful option for extended orbitomaxillary defect reconstruction. (C) 2016 American Association of Oral and Maxillofacial Surgeons
  • Koji Kanayama, Kazuhide Mineda, Takanobu Mashiko, Szu-Hsien Wu, Jingwei Feng, Kahori Kinoshita, Ataru Sunaga, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 139 (2) 365 - 374 0032-1052 2017/02 [Refereed][Not invited]
     
    Background: There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.Methods: Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood ( blood transfusion model) or plasma equivalent ( normovolemic hemodilution). Control rats were subjected to a sham procedure. Subsequently, a random-pattern skin flap ( 1.5 x 6 cm) was elevated on the back of each rat. Physiologic assessments of flap vascularity/viability were performed using laser Doppler spectrophotometry before and after flap elevation.Results: The normovolemic hemodilution group showed anemia ( hemoglobin, 9.5 +/- 0.8 g/dl) but less flow occlusion and greater flap survival ( 72.8 +/- 8.6 percent) compared with control ( 57.4 +/- 9.6 percent; p < 0.01) and blood transfusion ( 62.1 +/- 6.5 percent; p < 0.089) groups. In control and blood transfusion groups but not the normovolemic hemodilution group, blood flow was decreased and relative quantity of hemoglobin was increased toward the flap tip, indicating congestion. In control and blood transfusion groups, blood flow and tissue oxygen saturation dropped after flap elevation, but recovered by day 7; congestion gradually improved by day 7.Conclusions: The authors determined that congestion promoted necrosis and hemodilution reduced microcirculatory occlusion and increased blood flow and oxygenation in skin flaps. It was suggested that perioperative hemodilution is superior to blood transfusion in any flap operations unless there is a critical systemic need for blood transfusion.
  • Ataru Sunaga, Yasushi Sugawara, Hideaki Kamochi, Akira Gomi, Hirokazu Uda, Shunji Sarukawa, Kotaro Yoshimura
    Plastic and Reconstructive Surgery - Global Open 5 (12) e1617  2169-7574 2017 [Refereed][Not invited]
     
    Patients with syndromic craniosynostosis often require a large amount of cranial expansion to avoid intracranial hypertension, but the surgical procedure remains controversial. A patient of severe syndromic craniosynostosis with multiple bony defects and anomalous venous drainage at the occipital region was treated by multidirectional cranial distraction osteogenesis (MCDO) at the age of 8 months. Distraction started 5 days after surgery and ceased on postoperative day 16. The distraction devices were removed 27 days after completing distraction. After device removal, the increase of intracranial volume was 155 ml and the cephalic index was improved from 115.5 to 100.5. The resultant cranial shape was well maintained with minimal relapse at postoperative 9 months. In cases of syndromic craniosynostosis with multiple bony defects and/or anomalous venous drainage at the occipital region, expansion of the anterior cranium by MCDO is a viable alternative to conventional methods.
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Ataru Sunaga, Yasushi Sugawara, Hideaki Kamochi, Akira Gomi, Daekwan Chi, Rintaro Asahi, Masanori Mori, Shunji Sarukawa, Hirokazu Uda, Kotaro Yoshimura
    Plastic and Reconstructive Surgery - Global Open 5 (10) e1536  2169-7574 2017 [Refereed][Not invited]
     
    Background: Multidirectional cranial distraction osteogenesis (MCDO) is a procedure of ours developed earlier for treating craniosynostosis. However, the numerous bone flaps led to prolonged operative time and occasional bone detachment from dura. We have since simplified the osteotomy design. In treating sagittal synostosis, required bone flaps have been reduced to 11 (from ∼20). Methods: In a 2-year period (2014-2015), 5 boys with sagittal synostosis underwent MCDO using our simplified and fixed-form osteotomy. Mean age at surgery was 9.4 months (range, 8-11 months). Pre- and postoperative cranial morphology was assessed by cephalic index and by mid-sagittal vector analysis. Results: Improved cranial shape was confirmed by 3-dimensional CT scans and by mid-sagittal vector index. Mean preoperative cephalic index (68.7) progressively increased to means of 78.5 immediately after distraction device removal, 75.2 at postoperative month 6, and 75.1 at 1 year postoperatively. There were no major complications, although transient cerebrospinal fluid leakage and loosening of anchor pins occurred in 1 patient. Conclusions: Simplified MCDO has a number of advantages over conventional distraction procedures such as discretionary reshaping/expansion of cranium and predictable osteogenesis and is a valid treatment option for patients with sagittal synostosis.
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • Takanobu Mashiko, Szu-Hsien Wu, Jingwei Feng, Koji Kanayama, Kaori Kinoshita, Ataru Sunaga, Mitsunaga Narushima, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 139 (1) 79 - 90 0032-1052 2017/01 [Refereed][Not invited]
     
    Background: Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.Methods: Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emulsified by repeated transfer between two syringes through a small-hole connecter and then separated by mesh filtration into two portions: residual tissue of emulsified fat and filtrated fluid of emulsified fat. The four products were examined for cellular components.Results: Histologic and electron microscopic analyses revealed that squeezed fat and residual tissue of emulsified fat contained broken adipocytes and fragmented capillaries. Compared with centrifuged fat, the squeezed fat and residual fat products exhibited increased specific gravity and increased numbers of adipose-derived stem/stromal cells and endothelial cells per volume, suggesting successful cell/tissue condensation in both squeezed fat and residual tissue of emulsified fat. Although cell number and viability in the stromal vascular fraction were well maintained in both squeezed fat and residual fat, stromal vascular fraction culture assay showed that adipose-derived stromal cells were relatively damaged in residual tissue of emulsified fat but not in squeezed fat. By contrast, no adipose-derived stromal cells were cultured from filtrated fluid of emulsified fat.Conclusions: The authors' results demonstrated that mechanical micronization is easily conducted as a minimal manipulation procedure, which can condense the tissue by selectively removing adipocytes without damaging key components, such as adipose-derived stromal cells and endothelial cells. Depending on the extent of adipocyte removal, the product may be a useful therapeutic tool for efficient tissue volumization or therapeutic revitalization/ fertilization.
  • Kanayama Koji, Mineda Kazuhide, Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kinoshita Kahori, Sunaga Ataru, Yoshimura Kotaro
    Blood Congestion Can Be Rescued by Hemodilution in a Random-Pattern Skin Flap. 139 (2) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:There is no standard method to ensure survival of random-pattern skin flaps. The authors developed a rat anemia model to observe survival of random-pattern skin flaps after blood transfusion and hemodilution.;METHODS:Anemia was induced by withdrawal of 35 percent blood volume followed by compensation with the same amount of blood (blood transfusion model) or plasma equivalent (normovole
  • Kamochi Hideaki, Sarukawa Shunji, Uda Hirokazu, Nishino Hiroshi, Yoshimura Kotaro
    Orbitomaxillary Reconstruction Using a Combined Latissimus Dorsi Musculocutaneous and Scapular Angle Osseous Flap. 75 (2) 1531-5053 2017 [Not refereed][Not invited]
     
    :Immediate reconstruction of orbitomaxillary defects is challenging for head and neck reconstructive surgeons. The primary goals of orbitomaxillary reconstruction are to cover the skin and mucosal defects, fill the defect space, and reconstruct the natural facial contour. This report describes 2 patients who underwent extended orbitomaxillectomy and immediate reconstruction using a combined latiss
  • Mashiko Takanobu, Wu Szu-Hsien, Feng Jingwei, Kanayama Koji, Kinoshita Kaori, Sunaga Ataru, Narushima Mitsunaga, Yoshimura Kotaro
    Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques. 139 (1) 1529-4242 2017 [Not refereed][Not invited]
     
    BACKGROUND:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.;METHODS:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emul
  • Feng Jingwei, Mineda Kazuhide, Wu Szu-Hsien, Mashiko Takanobu, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Asahi Rintaro, Sunaga Ataru, Yoshimura Kotaro
    An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells. 7 (1) 2045-2322 2017 [Not refereed][Not invited]
     
    :For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D cultu
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • Uda H, Yoshimura K, Asahi R, Sarukawa S, Sunaga A, Kamochi H, Sugawara Y
    Plastic and reconstructive surgery. Global open 4 (12) e1123  2016/12 [Refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • 【形成外科の基礎研究-私の研究歴、および研究マインド育成に対する提言-】 形成外科の基礎研究 計画から完成まで
    吉村 浩太郎
    形成外科 克誠堂出版(株) 59 (12) 1264  0021-5228 2016/12 [Not refereed][Not invited]
  • 美容外科教育 個人史からの提言 美容外科教育について
    吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 156  0288-2027 2016/12 [Not refereed][Not invited]
  • Maxillary protrusionに対する分節骨切り術の適応について考える
    宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本美容外科学会会報 (一社)日本美容外科学会 38 (4) 171  0288-2027 2016/12 [Not refereed][Not invited]
  • Kamochi H, Sugawara Y, Uda H, Sarukawa S, Sunaga A, Yoshimura K
    Plastic and reconstructive surgery. Global open 4 (11) e1116  2016/11 [Refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Mashiko T, Minabe T, Yamakawa T, Araki J, Sano H, Yoshimura K
    Plastic and reconstructive surgery. Global open 4 (10) e1089  2016/10 [Refereed][Not invited]
  • Craniofacial surgeryがもたらした機能と整容の革命 顔面修復外科の興趣 注入治療による顔面修復
    吉村 浩太郎
    日本頭蓋顎顔面外科学会誌 (一社)日本頭蓋顎顔面外科学会 32 (3) 171  0914-594X 2016/10 [Not refereed][Not invited]
  • Hirokazu Uda, Yoko Katsuragi Tomioka, Syunji Sarukawa, Ataru Sunaga, Hideaki Kamochi, Yasusih Sugawara, Kotaro Yoshimura
    JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY 69 (9) 1178 - 1183 1748-6815 2016/09 [Refereed][Not invited]
     
    Background and aim: The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnervation occurs, patients who undergo single-pedicled DIEP (S-DIEP) flap or double-pedicled DIEP (D-DIEP) flap breast reconstruction would have similar levels of preoperative trunk flexion. To determine this, we investigated perioperative changes in trunk flexor muscle ability quantitatively using an isokinetic dynamometer in patients who had received S-DIEP or D-DIEP. Methods: Patients who underwent breast reconstruction with S-DIEP (n = 37) and D-DIEP (n = 30) were included in this study. Pre- and postoperative trunk flexor muscle ability was measured prospectively by an isokinetic dynamometer in all patients. Postoperative abdominal pain and stiffness, patients' activity, and incidence of bulging were also investigated. Results: Six months after surgery, the trunk flexor muscle ability recovered and did not significantly decrease subsequently in either group. This finding was consistent with the result that patients' activities and the incidence of bulging were similar between the two groups. Conclusions: Our results show that reinnervation of the rectus muscle can be confirmed at 6 months after DIEP flap elevation. Thus, we recommend D-DIEP flap without concern for abdominal wall weakness, especially in patients with multiple abdominal scars and who require breast tissue exceeding the amount of tissue that can be transferred with S-DIEP flap. (C) 2016 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
  • Pu LL, Yoshimura K
    Plastic and reconstructive surgery. Global open 4 (8) e830  2016/08 [Refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 吉村 浩太郎
    人工臓器 (一社)日本人工臓器学会 45 (1) 78  0300-0818 2016/06 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 須永 中, 去川 俊二, 宇田 宏一, 菅原 康志, 吉村 浩太郎
    日本顎変形症学会雑誌 (NPO)日本顎変形症学会 26 (2) 115  0916-7048 2016/05 [Not refereed][Not invited]
  • 加持 秀明, 去川 俊二, 杉浦 康史, 野口 忠秀, 西野 宏, 森 良之, 菅原 康志, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 148  1349-5747 2016/05 [Not refereed][Not invited]
  • 去川 俊二, 加持 秀明, 杉浦 康史, 野口 忠秀, 菅原 康志, 森 良之, 西野 宏, 吉村 浩太郎
    頭頸部癌 日本頭頸部癌学会 42 (2) 131  1349-5747 2016/05 [Not refereed][Not invited]
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Uda Hirokazu, Yoshimura Kotaro, Asahi Rintaro, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasushi
    Vertically Set Sombrero-shaped Abdominal Flap for Asian Breast Reconstruction after Skin-sparing Mastectomy. 4 (12) 2016 [Not refereed][Not invited]
     
    BACKGROUND:Immediate autologous breast reconstruction after skin-sparing mastectomy is an esthetically superior method, and a free abdominal flap is often used. However, in Asian patients, little redundant abdominal skin and thin subcutaneous tissue are common, necessitating the development of a more suitable flap design and setting. We devised a narrow flap, the sombrero-shaped flap (S-flap), set
  • Kamochi Hideaki, Sugawara Yasushi, Uda Hirokazu, Sarukawa Shunji, Sunaga Ataru, Yoshimura Kotaro
    A Novel Technique That Protects the Lips during Orthognathic Surgery. 4 (11) 2016 [Not refereed][Not invited]
     
    :Serious lip injuries can occur during orthognathic surgery. Although an Angle Wider device, which is commonly used during orthognathic surgery, provides some lip protection, it leaves more than half of the lip exposed to surgical instruments. Here, we describe a novel technique to protect the entire upper and lower lips during orthognathic surgery using a minilaparotomy wound edge protector (Lap-
  • Mashiko Takanobu, Minabe Toshiharu, Yamakawa Tomomi, Araki Jun, Sano Hitomi, Yoshimura Kotaro
    Platelet-derived Factor Concentrates with Hyaluronic Acid Scaffolds for Treatment of Deep Burn Wounds. 4 (10) 2016 [Not refereed][Not invited]
     
    :A deep burn wound is a critical condition that generally necessitates vascularized tissue coverage. We performed the injection of platelet-derived factor concentrates combined with non-cross-linked hyaluronic acid scaffolds for 2 patients with critical burn wounds with bone and tendon exposure and achieved successful healing. Hyaluronic acid was considered to have served as a controlled-release c
  • Pu Lee L Q, Yoshimura Kotaro
    The Fourth World Congress of the International Society of Plastic Regenerative Surgeons: Another Successful Scientific Forum for Regenerative Surgery. 4 (8) 2016 [Not refereed][Not invited]
  • Uda Hirokazu, Tomioka Yoko Katsuragi, Sarukawa Syunji, Sunaga Ataru, Kamochi Hideaki, Sugawara Yasusih, Yoshimura Kotaro
    Abdominal morbidity after single- versus double-pedicled deep inferior epigastric perforator flap use. 69 (9) 1878-0539 2016 [Not refereed][Not invited]
     
    BACKGROUND AND AIM:The reduced incidence of donor site morbidity after deep inferior epigastric perforator (DIEP) flap is because the rectus muscle and its fascia are preserved. However, no study has proved that trunk flexion recovers not by the compensatory effect of the contralateral rectus muscle but by reinnervation of the ipsilateral rectus muscle. We hypothesized that if sufficient reinnerva
  • Mineda K, Feng J, Ishimine H, Takada H, Doi K, Kuno S, Kinoshita K, Kanayama K, Kato H, Mashiko T, Hashimoto I, Nakanishi H, Kurisaki A, Yoshimura K
    Stem cells translational medicine 4 (12) 1511 - 1522 2157-6564 2015/12 [Refereed][Not invited]
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and reconstructive surgery. Global open 3 (11) e567  2015/11 [Not refereed][Not invited]
     
    Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and reconstructive surgery. Global open 3 (11) e567  2015/11 [Not refereed][Not invited]
     
    Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and reconstructive surgery. Global open 3 (11) e567  2015/11 [Not refereed][Not invited]
     
    Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and reconstructive surgery. Global open 3 (11) e567  2015/11 [Not refereed][Not invited]
     
    Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and reconstructive surgery. Global open 3 (11) e567  2169-7574 2015/11 [Refereed][Not invited]
     
    Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Takanobu Mashiko, Tanetaka Akizuki, Yorikatsu Watanabe, Ryo Sasaki, Munehiro Yokoyama, Kotaro Yoshimura, Kazuhide Mineda
    JOURNAL OF CRANIOFACIAL SURGERY 26 (6) 2025 - 2026 1049-2275 2015/09 [Refereed][Not invited]
  • Kotaro Yoshimura, Sydney R. Coleman
    CLINICS IN PLASTIC SURGERY 42 (3) 383 - 388 0094-1298 2015/07 [Refereed][Not invited]
     
    Recent technical and scientific advances in fat grafting procedures and concepts have improved predictability of fat grafting. Large-volume fat injection is gaining much attention as an attracting procedure for body contouring and reconstruction, but an increasing number of complications also has been recognized over the world. In this article, typical complications after fat grafting are described, as well as an explanation of how and why they occur, and how surgeons can avoid and treat complications.
  • Lee L. Q. Pu, Kotaro Yoshimura, Sydney R. Coleman
    CLINICS IN PLASTIC SURGERY 42 (3) 389 - 394 0094-1298 2015/07 [Refereed][Not invited]
     
    Autologous fat grafting is an exciting part of plastic and reconstructive surgery. Fat serves as a filler and its role in tissue regeneration will likely play a more important role in our specialty. As we learn more about the basic science of fat grafting and the standardized techniques and instruments used for fat grafting, this procedure alone or in conjunction with invasive procedures may be able to replace many operations that we perform currently. Its minimally invasive nature will benefit greatly our cosmetic and reconstructive patients, and may even achieve better clinical outcomes.
  • Lee L. Q. Pu, Kotaro Yoshimura, Sydney R. Coleman
    CLINICS IN PLASTIC SURGERY 42 (3) XIII - XIV 0094-1298 2015/07 [Refereed][Not invited]
  • Takanobu Mashiko, Yoshihisa Abo, Shinichiro Kuno, Kotaro Yoshimura
    DERMATOLOGIC SURGERY 41 (6) 755 - 758 1076-0512 2015/06 [Refereed][Not invited]
  • Takanobu Mashiko, Kotaro Yoshimura
    CLINICS IN PLASTIC SURGERY 42 (2) 181 - + 0094-1298 2015/04 [Refereed][Not invited]
     
    Autologous fat grafting has become an important procedure for volumization and revitalization, although clinical outcomes depend greatly on technique. It was revealed recently how grafted fat tissue survives, regenerates, or dies. Experimental results provided the underlying mechanism and clinical implications for therapeutic strategies to maximize the effects of fat grafting, minimize necrosis, and avoid oil cyst formation.
  • Shinichiro Kuno, Kotaro Yoshimura
    CLINICS IN PLASTIC SURGERY 42 (2) 191 - + 0094-1298 2015/04 [Refereed][Not invited]
     
    Aspirated fat contains unnecessary components such as water, oil, and blood cells. For better outcomes, tissue purification and condensation are useful, especially when injection volume to the recipient site is limited. Because aspirated fat is relatively poor in adipose-derived stem/stromal cells (ASCs), ASC condensation seems important for obtaining better regeneration and retention. Reducing tissue volume by removing some adipocytes or supplementation of stromal vascular fraction or ASCs can increase the ASC/adipocyte ratio in the graft. Clinical results of ASC supplementation remain controversial, but ASC condensation seems to lead to expanding applications of fat grafting into revitalization of stem cell-depleted tissue.
  • Lee L. Q. Pu, Kotaro Yoshimura, Sydney R. Coleman
    CLINICS IN PLASTIC SURGERY 42 (2) IX - X 0094-1298 2015/04 [Refereed][Not invited]
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Takanobu Mashiko, Kahori Kinoshita, Koji Kanayama, Jingwei Feng, Kotaro Yoshimura
    Plastic and Reconstructive Surgery - Global Open 3 (11) 2015 [Not refereed][Not invited]
     
    © 2015 The Authors. Published by Lippincott Williams & Wilkins on behalf of The American Society of Plastic Surgeons. Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injecting HA as intradermal struts with the skin fully stretched by the practitioner's fingers. The results of both NLFs in 10 patients suggest that this technique improves NLFs and maintain the effects more consistently than conventional techniques, although the effects of both methods were almost lost after 6 months. Selective and/or combined application of this technique may enhance the current approach to facial rejuvenation with dermal fillers.
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Mashiko Takanobu, Kinoshita Kahori, Kanayama Koji, Feng Jingwei, Yoshimura Kotaro
    Perpendicular Strut Injection of Hyaluronic Acid Filler for Deep Wrinkles. 3 (11) 2015 [Not refereed][Not invited]
     
    :Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injectin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Mashiko Takanobu, Kinoshita Kahori, Kanayama Koji, Feng Jingwei, Yoshimura Kotaro
    Perpendicular Strut Injection of Hyaluronic Acid Filler for Deep Wrinkles. 3 (11) 2015 [Not refereed][Not invited]
     
    :Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injectin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Jingwei Feng, Kentaro Doi, Shinichiro Kuno, Kazuhide Mineda, Harunosuke Kato, Kahori Kinoshita, Koji Kanayama, Takanobu Mashiko, Kotaro Yoshimura
    REGENERATIVE MEDICINE 10 (6) 699 - 708 1746-0751 2015 [Refereed][Not invited]
     
    Background: Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies. Materials & methods: Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures. Results: It retained some capillaries and ASCs, but most adipocytes were removed. The presence of viable ASCs, vascular endothelial cells was confirmed and ASCs of MCAM kept intact mesenchymal differentiation capacity. In diabetic mice, skin wounds treated with MCAM showed significantly accelerated healing compared with phosphate-buffered saline-treated ones. Conclusion: The proven potential of MCAM to accelerate healing in ischemic diabetic ulcers may offer a simple, safe and minimally invasive means for tissue repair and revitalization.
  • Mineda Kazuhide, Feng Jingwei, Ishimine Hisako, Takada Hitomi, Doi Kentaro, Kuno Shinichiro, Kinoshita Kahori, Kanayama Koji, Kato Harunosuke, Mashiko Takanobu, Hashimoto Ichiro, Nakanishi Hideki, Kurisaki Akira, Yoshimura Kotaro
    Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel. 4 (12) 2157-6564 2015 [Not refereed][Not invited]
     
    UNLABELLED:Three-dimensional culture of mesenchymal stem/stromal cells for spheroid formation is known to enhance their therapeutic potential for regenerative medicine. Spheroids were prepared by culturing human adipose-derived stem/stromal cells (hASCs) in a non-cross-linked hyaluronic acid (HA) gel and compared with dissociated hASCs and hASC spheroids prepared using a nonadherent dish. Prelimin
  • Mashiko Takanobu, Kinoshita Kahori, Kanayama Koji, Feng Jingwei, Yoshimura Kotaro
    Perpendicular Strut Injection of Hyaluronic Acid Filler for Deep Wrinkles. 3 (11) 2015 [Not refereed][Not invited]
     
    :Although various injection techniques of hyaluronic acid (HA) filler for facial rejuvenation have been developed, correction of deep wrinkles/grooves, such as the nasolabial fold (NLF), with intradermal or subdermal injections remains difficult. We tested the intradermal HA injection method to place multiple HA struts by (1) inserting a small needle perpendicularly to the wrinkle and (2) injectin
  • Feng Jingwei, Doi Kentaro, Kuno Shinichiro, Mineda Kazuhide, Kato Harunosuke, Kinoshita Kahori, Kanayama Koji, Mashiko Takanobu, Yoshimura Kotaro
    Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer. 10 (6) 1746-076X 2015 [Not refereed][Not invited]
     
    BACKGROUND:Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.;MATERIALS & METHODS:Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.;RESULTS:It retain
  • Kahori Kinoshita, Hisako Ishimine, Kenshiro Shiraishi, Harunosuke Kato, Kentaro Doi, Shinichiro Kuno, Koji Kanayama, Kazuhide Mineda, Takanobu Mashiko, Jingwei Feng, Keiichi Nakagawa, Akira Kurisaki, Satoshi Itami, Kotaro Yoshimura
    CELLS TISSUES ORGANS 200 (3-4) 240 - 252 1422-6405 2014 [Refereed][Not invited]
     
    Ionizing radiation is often used to treat progressive neoplasms. However, the consequences of long-term radiation exposure to healthy skin tissue are poorly understood. We aimed to evaluate the short-and long-term radiation damage to healthy skin of the same irradiation given either as single or fractional doses. C57BL/J6 mice were randomly assigned to one of three groups: a control and two exposure groups (5 Gy x2 or 10 Gy x1). The inguinal area was irradiated (6-MeV beam) 1 week after depilation in the treatment groups. Skin samples were evaluated macroscopically and histologically for up to 6 months after the final exposure. After anagen hair follicle injury by irradiation, hair cycling resumed in both groups, but hair graying was observed in the 10 Gy x1 group but not in the 5 Gy x2 group, suggesting the dose of each fractional exposure is more relevant to melanocyte stem cell damage than the total dose. On the other hand, in the long term, the fractional double exposures induced more severe atrophy and capillary reduction in the dermis and subcutis, suggesting fractional exposure may cause more depletion of tissue stem cells and endothelial cells in the tissue. Thus, our results indicated that there were differences between the degrees of damage that occurred as a result of a single exposure compared with fractional exposures to ionizing radiation: the former induces more severe acute injury to the skin with irreversible depigmentation of hairs, while the latter induces long-term damage to the dermis and subcutis. (C) 2015 S. Karger AG, Basel
  • Kentaro Doi, Shinsuke Tanaka, Hideo Iida, Hitomi Eto, Harunosuke Kato, Noriyuki Aoi, Shinichiro Kuno, Toshitsugu Hirohi, Kotaro Yoshimura
    Journal of Tissue Engineering and Regenerative Medicine 7 (11) 864 - 870 1932-6254 2013/11 [Refereed][Not invited]
     
    The heterogeneous stromal vascular fraction (SVF), containing adipose-derived stem/progenitor cells (ASCs), can be easily isolated through enzymatic digestion of aspirated adipose tissue. In clinical settings, however, strict control of technical procedures according to standard operating procedures and validation of cell-processing conditions are required. Therefore, we evaluated the efficiency and reliability of an automated system for SVF isolation from adipose tissue. SVF cells, freshly isolated using the automated procedure, showed comparable number and viability to those from manual isolation. Flow cytometric analysis confirmed an SVF cell composition profile similar to that after manual isolation. In addition, the ASC yield after 1week in culture was also not significantly different between the two groups. Our clinical study, in which SVF cells isolated with the automated system were transplanted with aspirated fat tissue for soft tissue augmentation/reconstruction in 42 patients, showed satisfactory outcomes with no serious side-effects. Taken together, our results suggested that the automated isolation system is as reliable a method as manual isolation and may also be useful in clinical settings. Automated isolation is expected to enable cell-based clinical trials in small facilities with an aseptic room, without the necessity of a good manufacturing practice-level cell processing area. © 2012 John Wiley & Sons, Ltd.
  • Hitomi Eto, Hisako Ishimine, Kahori Kinoshita, Kanako Watanabe-Susaki, Harunosuke Kato, Kentaro Doi, Shinichiro Kuno, Akira Kurisaki, Kotaro Yoshimura
    Stem Cells and Development 22 (6) 985 - 997 1547-3287 2013/03 [Refereed][Not invited]
     
    Adipose tissue (AT) is composed of mature adipocytes and stromal vascular fraction (SVF) cells, including adipose stem/stromal cells (ASCs). We characterized hematopoietic cells residing in human nonobese AT by analyzing the SVF isolated from human lipoaspirates and peripheral blood (PB). Flow cytometry revealed that AT-resident hematopoietic cells consisted of AT-resident macrophages (ATMs) or lymphocytes with a negligible number of granulocytes. AT-resident lymphocytes were composed of helper T cells and natural killer cells. Almost no B cells and few cytotoxic T cells were observed in nonobese AT. More than 90% of ATMs were M2 state CD206+ macrophages (CD45 +/CD14+) that were located in the periendothelium or interstitial spaces between adipocytes. We also discovered a novel subpopulation of CD34+/CD206+ ATMs (11.1% of CD206+ATMs) that localized in the perivascular region. Microarray of noncultured CD34 +/CD206+ ATMs, CD34-/CD206+ ATMs, CD45-/CD31-/CD34+ ASCs, and PB-derived circulating monocytes revealed that CD34+/CD206+ ATMs shared characteristics with ASCs and circulating monocytes. Unlike CD34 -/CD206+ ATMs, CD34+/CD206+ ATMs could grow in adherent culture and were capable of differentiating into multiple mesenchymal (adipogenic, osteogenic, and chondrogenic) lineages, similar to ASCs. CD34+/CD206+ ATMs grew rapidly and lost expression of CD45, CD14, and CD206 by passage 3, which resulted in a similar expression profile to ASCs. Thus, this novel ATM subpopulation (CD45+/CD14 +/CD34+/CD206+) showed distinct biological properties from other ATMs and circulating monocytes/macrophages. The CD34 +/CD206+ ATMs possessed characteristics similar to ASCs, including adherence, localization, morphology, and mesenchymal multipotency. This AT-resident subpopulation may have migrated from the bone marrow and may be important to tissue maintenance and remolding. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
  • Takashi Nagase, Tomoko Akase, Hiromi Sanada, Takeo Minematsu, Ai Ibuki, Lijuan Huang, Mayumi Asada, Kotaro Yoshimura, Miki Nagase, Tsutomu Shimada, Masaki Aburada, Gojiro Nakagami, Junko Sugama
    Aging cell 12 (1) 50 - 7 1474-9718 2013/02 [Refereed][Not invited]
     
    Aging is accelerated, at least in part, by pathological condition such as metabolic syndrome (MetS), and various molecular pathways such as oxidative stress are common mediators of aging and MetS. We previously developed the aging-like skin model by single ultraviolet (UV) irradiation on the MetS model mice. Recent studies revealed that mineralocorticoid receptor (MR) signaling plays a pivotal role for various tissue inflammation and damages in MetS. Although previous studies reported that MR is expressed in the skin and that overexpression of MR in the skin resulted in the skin atrophy, the physiological or pathological functions of MR in the skin are not fully elucidated. Here, we show the involvement of MR signaling in the aging-like skin changes in our own model. Elevations of oxidative stress and inflammation markers were observed in the MetS mice, and the UV-evoked aging-like skin damages were attenuated by topical antioxidant. MR expression was higher in the MetS mouse skin, and notably, expression of its effecter gene Sgk1 was significantly upregulated in the aging-like skin in the UV-irradiated MetS mice. Furthermore, topical application of MR antagonist spironolactone suppressed Sgk1 expression, oxidative stress, inflammation, and the aging-like changes in the skin. The 2-week UV onto the non-MetS mice, the more usual photoaging model, resulted in the skin damages mostly equivalent to the MetS mice with single UV, but they were not associated with upregulation of MR signaling. Our studies suggested an unexpected role of MR signaling in the skin aging in MetS status.
  • Noriyuki Aoi, Keita Inoue, Toshihiro Chikanishi, Ryoji Fujiki, Hanako Yamamoto, Harunosuke Kato, Hitomi Eto, Kentaro Doi, Satoshi Itami, Shigeaki Kato, Kotaro Yoshimura
    STEM CELLS TRANSLATIONAL MEDICINE 1 (8) 615 - 626 2157-6564 2012/08 [Refereed][Not invited]
     
    Dermal papilla cells (DPCs) have the potential to induce differentiation of epithelial stem cells into hair, and Wnt signaling is deeply involved in the initiation process. The functional limitation of expanded adult DPCs has been a difficult challenge for cell-based hair regrowth therapy. We previously reported that 1 alpha,25-dihydroxyvitamin D-3 (VD3) upregulates expression of transforming growth factor (TGF)-beta 2 and alkaline phosphatase (ALP) activity, both features of hair-inducing human DPCs (hDPCs). In this study, we further examined the effects and signaling pathways associated with VD3 actions on DPCs. VD3 suppressed hDPC proliferation in a dose-dependent, noncytotoxic manner. Among the Wnt-related genes investigated, Wnt10b expression was significantly upregulated by VD3 in hDPCs. Wnt10b upregulation, as well as upregulation of ALPL (ALP, liver/bone/kidney) and TGF-beta 2, by VD, was specific in hDPCs and not detected in human dermal fibroblasts. Screening of paracrine or endocrine factors in the skin indicated that all-trans retinoic acid (atRA) upregulated Wnt10b gene expression, although synergistic upregulation (combined atRA and VD3) was not seen. RNA interference with vitamin D receptor (VDR) revealed that VD3 upregulation of Wnt10b, ALPL, and TGF-beta 2 was mediated through the genomic VDR pathway. In a rat model of de novo hair regeneration by murine DPC transplantation, pretreatment with VD3 significantly enhanced hair folliculogenesis. Specifically, a greater number of outgrowing hair shafts and higher maturation of regenerated follicles were observed. Together, these data suggest that VD3 may promote functional differentiation of DPCs and be useful in preserving the hair follicle-inductive capacity of cultured DPCs for hair regeneration therapies. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:615-626
  • Noriyuki Aoi, Keita Inoue, Toshihiro Chikanishi, Ryoji Fujiki, Hanako Yamamoto, Harunosuke Kato, Hitomi Eto, Kentaro Doi, Satoshi Itami, Shigeaki Kato, Kotaro Yoshimura
    STEM CELLS TRANSLATIONAL MEDICINE 1 (8) 615 - 626 2157-6564 2012/08 [Refereed][Not invited]
     
    Dermal papilla cells (DPCs) have the potential to induce differentiation of epithelial stem cells into hair, and Wnt signaling is deeply involved in the initiation process. The functional limitation of expanded adult DPCs has been a difficult challenge for cell-based hair regrowth therapy. We previously reported that 1 alpha,25-dihydroxyvitamin D-3 (VD3) upregulates expression of transforming growth factor (TGF)-beta 2 and alkaline phosphatase (ALP) activity, both features of hair-inducing human DPCs (hDPCs). In this study, we further examined the effects and signaling pathways associated with VD3 actions on DPCs. VD3 suppressed hDPC proliferation in a dose-dependent, noncytotoxic manner. Among the Wnt-related genes investigated, Wnt10b expression was significantly upregulated by VD3 in hDPCs. Wnt10b upregulation, as well as upregulation of ALPL (ALP, liver/bone/kidney) and TGF-beta 2, by VD, was specific in hDPCs and not detected in human dermal fibroblasts. Screening of paracrine or endocrine factors in the skin indicated that all-trans retinoic acid (atRA) upregulated Wnt10b gene expression, although synergistic upregulation (combined atRA and VD3) was not seen. RNA interference with vitamin D receptor (VDR) revealed that VD3 upregulation of Wnt10b, ALPL, and TGF-beta 2 was mediated through the genomic VDR pathway. In a rat model of de novo hair regeneration by murine DPC transplantation, pretreatment with VD3 significantly enhanced hair folliculogenesis. Specifically, a greater number of outgrowing hair shafts and higher maturation of regenerated follicles were observed. Together, these data suggest that VD3 may promote functional differentiation of DPCs and be useful in preserving the hair follicle-inductive capacity of cultured DPCs for hair regeneration therapies. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:615-626
  • Takeo Minematsu, Lijuan Huang, Ai Ibuki, Gojiro Nakagami, Tomoko Akase, Junko Sugama, Takashi Nagase, Kotaro Yoshimura, Hiromi Sanada
    Biological research for nursing 14 (3) 242 - 9 1099-8004 2012/07 [Refereed][Not invited]
     
    Obesity is recognized as a risk factor for delayed cutaneous wound healing. The authors hypothesized that the secretion of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) from subcutaneous adipose tissue correlates with disorder of the healing process in obese subjects. Findings from previous studies on the expression of MMPs and TIMPs in obese adipose tissue are inconsistent. Since these conflicting results could be due to the effect of several intrinsic factors, the authors conducted a simple in vitro experiment to clarify the change in profile of MMPs and TIMPs in excessively matured adipocytes. The authors cultured the induced adipocytes under conditions of high or low glucose and with or without insulin supplementation. Oil red O staining and its dye extraction assay revealed excessive lipid accumulation in high glucose and insulin-supplemented adipocytes. Additionally, there was altered expression of adipokines, similar to the change in adipose tissue in obese subjects. Under these conditions, the expression/activity of MMP8 was promoted and the expression of MMP3 and TIMP3 was inhibited. Further studies to determine the effect of other obesity-related factors, such as insulin resistance, on MMPs and TIMPs are required.
  • Hitomi Eto, Harunosuke Kato, Hirotaka Suga, Noriyuki Aoi, Kentaro Doi, Shinichiro Kuno, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 129 (5) 1081 - 1092 0032-1052 2012/05 [Refereed][Not invited]
     
    Background: Clinical outcomes following fat grafting are variable and technique dependent, and it is unknown how the graft is revascularized. The authors recently observed that living and dead adipocytes can be differentiated not with hematoxylin and eosin staining but with immunohistochemistry for perilipin. Methods: The viability of cellular components (adipocytes, adipose stem/stromal/progenitor cells, vascular endothelial cells, and hematopoietic cells) in human adipose tissue was evaluated using (1) stored lipoaspirates, (2) cultured cells, and (3) organ-cultured adipose tissue. In addition, the groin fat pad (150 to 200 mg) in mice was transplanted under the scalp, and the graft was stained at 0, 1, 2, 3, 5, 7, or 14 days. Results: In vitro studies revealed that adipocytes are most susceptible to death under ischemic conditions, although adipose-derived stromal cells can remain viable for 3 days. The in vivo study indicated that most adipocytes in the graft began to die on day 1, and only some of the adipocytes located within 300 mu m of the tissue edge survived. The number of proliferating cells increased from day 3, and an increase in viable adipocyte area was detected from day 7, suggesting that repair/regeneration of the dead tissue had begun. Conclusions: The authors show convincing evidence of very dynamic remodeling of adipose tissue after nonvascularized grafting. The authors observed three zones from the periphery to the center of the graft: the surviving area (adipocytes survived), the regenerating area (adipocytes died, adipose-derived stromal cells survived, and dead adipocytes were replaced with new ones), and the necrotic area (both adipocytes and adipose-derived stromal cells died). (Plast. Reconstr. Surg. 129: 1081, 2012.)
  • J. Peter Rubin, Devin Coon, Margarita Zuley, Jonathan Toy, Yuko Asano, Masakazu Kurita, Noriyuki Aoi, Kiyonori Harii, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 129 (5) 1029 - 1038 0032-1052 2012/05 [Refereed][Not invited]
     
    Background: One issue in the adoption of autologous fat transfer to the breast is concern over mammographic changes that may obscure cancer detection. The authors compared mammographic changes following fat grafting to the breast with changes seen after breast reduction. Methods: Twenty-seven women who had normal preoperative mammograms were treated with fat grafting to the breast, including admixing of autologous adipose stem cells with the fat graft, for cosmetic augmentation. Repeated mammograms were performed 12 months after surgery. As a control group, postsurgical mammograms from 23 reduction mammaplasty patients were compared. Eight academic breast imaging radiologists reviewed each mammogram in a blinded fashion. Outcomes analysis accounting for individual radiologist's tendencies was performed using generalized estimating equations. Results: The average volume of fat injected per patient was 526.5 cc. Fifty mammograms (27 lipotransfer, 23 breast reduction) were assessed. Differences in interpretation among individual radiologists were consistently observed (p < 0.10). Differences in abnormality rates were nonsignificant for oil cysts, benign calcifications, and calcifications warranting biopsy. Scarring (p < 0.001) and masses requiring biopsy (p < 0.001) were more common in the reduction cohort. Breast Imaging Reporting and Data System scores were higher after breast reduction (p < 0.001). Significant differences in the recommended follow-up time were also seen (p < 0.01). Conclusions: Compared with reduction mammaplasty, a widely accepted procedure, fat grafting to the breast produces fewer radiographic abnormalities with a more favorable Breast Imaging Reporting and Data System score and less aggressive follow-up recommendations by breast radiologists. (Plast. Reconstr. Surg. 129: 1029, 2012.)
  • Ai Ibuki, Tomoko Akase, Takashi Nagase, Takeo Minematsu, Gojiro Nakagami, Motoko Horii, Hiroshi Sagara, Takashi Komeda, Masayuki Kobayashi, Tsutomu Shimada, Masaki Aburada, Kotaro Yoshimura, Junko Sugama, Hiromi Sanada
    Experimental dermatology 21 (3) 178 - 83 0906-6705 2012/03 [Refereed][Not invited]
     
    The purpose of this study was to test the hypothesis that obese diabetic mice exhibit marked skin fragility, which is caused by increased oxidative stress and increased matrix metalloproteinase (MMP) gene expression in the subcutaneous adipose tissue. Scanning electron microscopy of skin samples from Tsumura-Suzuki obese diabetic (TSOD) mice revealed thinner collagen bundles, and decreased density and convolution of the collagen fibres. Furthermore, skin tensile strength measurements confirmed that the dorsal skin of TSOD mice was more fragile to tensile force than that of non-obese mice. The mRNA expressions of heme oxygenase 1 (Hmox1), a marker of oxidative stress, Mmp2 and Mmp14 were increased in the adipose tissue of TSOD mice. Antioxidant experiments were subsequently performed to determine whether the changes in collagen fibres and skin fragility were caused by oxidative stress. Strikingly, oral administration of the antioxidant dl-α-tocopherol acetate (vitamin E) decreased Hmox1, Mmp2 and Mmp14 mRNA expressions, and improved the skin tensile strength and structure of collagen fibres in TSOD mice. These findings suggest that the skin fragility in TSOD mice is associated with dermal collagen damage and weakened tensile strength, and that oxidative stress and MMP overexpression in the subcutaneous adipose tissue may, at least in part, affect dermal fragility via a paracrine pathway. These observations may contribute to novel clinical interventions, such as dietary supplementation with antioxidants or application of skin cream containing antioxidants, which may overcome skin fragility in obese patients with diabetes.
  • Jun Araki, Masahiro Jona, Hitomi Eto, Noriyuki Aoi, Harunosuke Kato, Hirotaka Suga, Kentaro Doi, Yutaka Yatomi, Kotaro Yoshimura
    TISSUE ENGINEERING PART C-METHODS 18 (3) 176 - 185 1937-3384 2012/03 [Refereed][Not invited]
     
    Platelet-rich plasma (PRP) has been clinically used as an easily prepared growth factor cocktail that can promote wound healing, angiogenesis, and tissue remodeling. However, the therapeutic effects of PRP are still controversial, due partly to the lack of optimized and standardized preparation protocols. We used whole blood (WB) samples to optimize the preparation protocols for PRP, white blood cell-containing (W-PRP), platelet-concentrated plasma (PCP), and noncoagulating platelet-derived factor concentrate (PFC). PRP and W-PRP were most efficiently collected by 10 min centrifugation in a 15-mLconical tube at 230-270 g and 70 g, respectively. To prepare PCP, platelets were precipitated by centrifugation of PRP at >2300 g, 90% of supernatant plasma was removed, and the platelets were resuspended. For preparation of noncoagulating PFC, the supernatant was replaced with one-tenth volume of saline, followed by platelet activation with thrombin. Platelet (before activation) and platelet-derived growth factor (PDGF)-BB (after activation) concentrations in PCP were approximately 20 times greater than those in WB, whereas PFC contained a 20-times greater concentration of platelets before platelet activation and a 50-times greater concentration of PDGF-BB without formation of a fibrin gel after platelet activation than WB. Surprisingly, total PDGF-BB content in the PFC was twice that of activated WB, which suggested that a substantial portion of the PDGF-BB became trapped in the fibrin glue, and replacement of plasma with saline is crucial for maximization of platelet-derived factors. As an anticoagulant, ethylene di-amine tetra-acetic acid disodium inhibited platelet aggregation more efficiently than acid citrate dextrose solution, resulting in higher nonaggregated platelet yield and final PDGF-BB content. These results increase our understanding of how to optimize and standardize preparation of platelet-derived factors at maximum concentrations.
  • Mayumi Asada, Gojiro Nakagami, Takeo Minematsu, Takashi Nagase, Tomoko Akase, Lijuan Huang, Kotaro Yoshimura, Hiromi Sanada
    Experimental dermatology 21 (2) 118 - 22 0906-6705 2012/02 [Refereed][Not invited]
     
    Wound infection is a form of host damage resulting from an imbalance in pathogen virulence and the host immune response. However, at present, diagnosis is based solely on bacterial numbers or inflammatory signs and is therefore not precise. Thus, infection diagnosis requires indicators of both of these factors. We focused on wound fluid because it includes both bacteria and host cells. The purpose of this study was to establish biomarkers that reflect both bacterial and host factors using the reverse transcription-polymerase chain reaction method on the centrifugal precipitation of wound fluids (wound fluid RT-PCR). We created full thickness wounds in animal models of the three groups: control, colonization and infection, which were conditioned by administration of different concentrations of Pseudomonas aeruginosa dispersion. Messenger RNA expression in bacteria and host cells was analysed. Expression of bacterial housekeeping genes was detected in the samples in the colonization and infection groups. Expression of host housekeeping genes was detected in all samples from the three groups. Expression of toxA, encoding the virulence factor exotoxin A, was detected in 90% of samples in the infection group only. Expression of Foxp3, encoding the transcription factor forkhead box P3, was detected in 100% of samples only in the colonization group. These results revealed that wound fluid RT-PCR analysis reflected both bacterial virulence and the host immune status, and we determined the combination of novel biomarkers that can discriminate these three groups. We anticipate that wound fluid RT-PCR could be applied in the future to diagnose wound infection.
  • Hitomi Eto, Hirotaka Suga, Noriyuki Aoi, Harunosuke Kato, Kentaro Doi, Shinichiro Kuno, Yasuhiko Tabata, Kotaro Yoshimura
    LABORATORY INVESTIGATION 92 (2) 214 - 223 0023-6837 2012/02 [Refereed][Not invited]
     
    Although hypertrophic scars (HTSs) and keloids are challenging problems, their pathogenesis is not well understood, making therapy difficult. We showed that matrix metalloproteinase (MMP)-1 expression was downregulated in HIS compared with normal skin from the same patients, whereas type 1 and 3 collagen and transforming growth factor-beta (TGF-beta) were upregulated. These differences, however, were not seen in cultured fibroblasts, suggesting the involvement of microenvironmental factors in the pathogenesis of HIS. Fibroblast growth factor-2 (FGF-2) highly upregulated the expression of MMP-1 and hepatocyte growth factor (HGF) in both HTS-derived and control fibroblasts; the upregulation was reversed by extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) inhibitors. An animal study using human HTS tissue implanted into nude mice indicated that controlled-release FGF-2 resulted in significantly less weight and decreased hydroxyproline content in HTS. Degradation of collagen fibers in FGF-2-treated HTS was also confirmed histologically. Western blotting showed that FGF-2-treated HIS expressed significantly higher MMP-1 protein than control. Decreased MMP-1 expression may be an important transcriptional change in HTS, and its reversal as well as upregulation of HGF by FGF-2 could be a new therapeutic approach for HIS. Laboratory Investigation (2012) 92, 214-223; doi:10.1038/labinvest.2011.127; published online 26 September 2011
  • Noriyuki Aoi, Keita Inoue, Harunosuke Kato, Hirotaka Suga, Takuya Higashino, Hitomi Eto, Kentaro Doi, Jun Araki, Takuya Iida, Tomoya Katsuta, Kotaro Yoshimura
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 6 (2) 85 - 95 1932-6254 2012/02 [Refereed][Not invited]
     
    Dermal papilla cells (DPCs) interact with epithelial stem cells and induce hair folliculogenesis. Cell-based therapies using expanded DPCs for hair regeneration have been unsuccessful in humans. Two major challenges remain: first, expanded DPCs obtained from adult hair follicles have functional limitations; second, a clinically applicable method is needed for transplanting DPCs. This study aimed to identify an efficient, minimally invasive and economical DPC transplantation procedure for use in clinical settings. Five clinically applicable transplantation procedures were tested, termed the Pinhole, Laser, Slit, Non-vascularized sandwich (NVS) and Hemi-vascularized sandwich (HVS) methods. Labelled rat dermal papilla tissue was transplanted into rat sole skin, and hair follicle regeneration was evaluated histologically. Regenerated follicles and labelled DPCs were detected for all methods, although some follicles showed abnormal growth, i.e. a cystic or inverted appearance. The HVS method, pioneered here, resulted in significantly larger number of regenerated follicles that were more mature and regular than those observed using the other methods. Moreover, hair growth was detected after expanded adult-derived DPC transplantation using the HVS method. These results suggest that direct contact of epithelial and dermal components and better vascularization/oxygenation of the recipient site are critical for hair regeneration in cell-based therapies. Copyright (C) 2011 John Wiley & Sons, Ltd.
  • Asada, M., Nakagami, G., Minematsu, T., Nagase, T., Akase, T., Huang, L., Yoshimura, K., Sanada, H.
    Wound Repair and Regeneration 20 (4) 601 - 610 1067-1927 2012 [Refereed][Not invited]
  • Kotaro Yoshimura, Hitomi Eto, Harunosuke Kato, Kentaro Doi, Noriyuki Aoi
    Regenerative Medicine 6 (6) 33 - 41 1746-0751 2011/11 [Refereed][Not invited]
     
    Many features of adipose stem/progenitor cells, including their physiological functions and localization, have been clarified in the past decade. Adipose tissue turns over very slowly, with perivascular progenitor cells differentiating into new adipocytes to replace dead adipocytes. A number of clinical trials using freshly isolated or cultured adipose-derived stromal cells containing adipose progenitor/stem cells are ongoing. Therapeutic use of adipose stem/progenitor cells has been shown to promote angiogenesis and adipose tissue regeneration. Identification of adipocyte-releasing factors upon apoptosis/necrosis would be a breakthrough and could lead to the next stage for adipose tissue regeneration. Activation of precursors in perichondrium and periosteum shows a dramatic neogenesis by simple injection and is an ideal example of in situ tissue engineering. The 'hit and catch' strategy using a mobilizer of bone-marrow stem/progenitor cells (hit) and attractants to lead the cells to proper homing into the target tissue (catch) may be the future of stem cell manipulation. Careful design of the microenvironment, cell delivery protocol to avoid unexpected behavior and induce maximal potential, and selection of target diseases, will be critical to the success of clinical applications of adipose-derived stromal cells. © 2011 Future Medicine Ltd.
  • Eri Ohto-Fujita, Tomohiro Konno, Miho Shimizu, Kazuhiko Ishihara, Toshihiro Sugitate, Jun Miyake, Kotaro Yoshimura, Kaori Taniwaki, Takashi Sakurai, Yukio Hasebe, Yoriko Atomi
    CELL AND TISSUE RESEARCH 345 (1) 177 - 190 0302-766X 2011/07 [Refereed][Not invited]
     
    We have found that a water-soluble alkaline-digested form of eggshell membrane (ASESM) can provide an extracellular matrix (ECM) environment for human dermal fibroblast cells (HDF) in vitro. Avian eggshell membrane (ESM) has a fibrous-meshwork structure and has long been utilized as a Chinese medicine for recovery from burn injuries and wounds in Asian countries. Therefore, ESMis expected to provide an excellent natural material for biomedical use. However, such applications have been hampered by the insolubility of ESM proteins. We have used a recently developed artificial cell membrane biointerface, 2-methacryloyloxyethyl phosphorylcholine polymer (PMBN) to immobilize ASESM proteins. The surface shows a fibrous structure under the atomic force microscope, and adhesion of HDF to ASESM is ASESM-dose-dependent. Quantitative mRNA analysis has revealed that the expression of type III collagen, matrix metalloproteinase-2, and decorin mRNAs is more than two-fold higher when HDF come into contact with a lower dose ASESM proteins immobilized on PMBN surface. A particle-exclusion assay with fixed erythrocytes has visualized secreted water-binding molecules around the cells. Thus, HDF seems to possess an ECM environment on the newly designed PMBN-ASESM surface, and future applications of the ASESM-PMBN system for biomedical use should be of great interest.
  • Takeo Minematsu, Yuko Yamamoto, Takashi Nagase, Ayumi Naito, Kimie Takehara, Shinji Iizaka, Kazunori Komagata, Lijuan Huang, Gojiro Nakagami, Tomoko Akase, Makoto Oe, Kotaro Yoshimura, Tadao Ishizuka, Junko Sugama, Hiromi Sanada
    Journal of dermatological science 62 (3) 160 - 8 0923-1811 2011/06 [Refereed][Not invited]
     
    BACKGROUND: Skin maceration is recognized as a risk factor for the development of certain skin lesions. In health care settings, incontinence-associated skin maceration is highly prevalent in the elderly. However, the effect of senescence on maceration has not been fully elucidated. OBJECTIVE: To reveal the enhancement of the maceration-induced ultrastructural alteration and barrier function of the epidermis by aging. METHODS: Skin maceration was reproduced by exposure to agarose gel in human and rat. The ultrastructural alterations in human and rat tissue were observed by transmission electron microscopy. The skin barrier function was evaluated by noninvasive methods in human, and by the transdermal penetration of small- and large-fluorescent molecules in rat. In order to reveal the effect of aging on the skin maceration, we compared these parameters between young and aged rats. RESULTS: In macerated skin, we observed expansion of the interstices of the stratum corneum, spinosum, and basale of the epidermis; disruption of the intercellular lipid structure in the stratum corneum; a decreased number of cell processes in the stratum spinosum and basale. The transdermal penetration test in the rat using two types of fluorescein indicated that maceration disrupted skin barrier function. Furthermore, senescence-enhanced ultrastructural and functional alterations were revealed in the rodent studies. CONCLUSION: This study demonstrates that aging enhances skin maceration. Considering that maceration is a risk factor for the skin damage, the development of technology to promote skin barrier recovery after maceration in the elderly is warranted.
  • Hitomi Eto, Hirotaka Suga, Keita Inoue, Noriyuki Aoi, Harunosuke Kato, Jun Araki, Kentaro Doi, Takuya Higashino, Kotaro Yoshimura
    AMERICAN JOURNAL OF PATHOLOGY 178 (5) 2322 - 2332 0002-9440 2011/05 [Refereed][Not invited]
     
    Based on the analysis of exudates from injured adipose tissue, we prepared a mixture containing the injury-associated growth factors at the same proportion as the exudates, named adipose injury cocktail (AIC). We hypothesized that AIC induces a series of regenerating and angiogenic processes without actual wounding. The purpose of this study is to elucidate the therapeutic potentials of AIC. AIC preferentially activated adipose-derived stem/progenitor/stromal cells (ASCs) to proliferate, migrate, and form networks compared with vascular endothelial cells, whereas vascular endothelial growth factor did not induce mitogenesis or chemotaxis in human ASCs. Each component growth factor of AIC was differently responsible for the ASC activation. AIC-treated ASCs tended to differentiate into adipocytes or vessel-constituting cells rather than into other cell types. In ischemic adipose tissues of mice, induced by either a surgical intervention or diabetes, AIC administration enhanced proliferation, especially of CD31(-)/CD34(+) ASCs, and mitigated tissue hypoxia by increasing capillary density and reducing fibrogenesis. These results suggest that AIC may have therapeutic potentials for various ischemic/hypoxic conditions by inducing adipose remodeling and neovascularization through activation of ASCs and other cells. Treatment with AIC has many advantages over cell-based therapies regarding morbidity, cost, and physical risks and may be used as an alternative therapy for improving tissue oxygen. (Am J Pathol 2011, 178:2322-2332; 10.1016/j.ajpath.2011.01.032)
  • Harunosuke Kato, Jun Araki, Hitomi Eto, Kentaro Doi, Rintaro Hirai, Shinichiro Kuno, Takuya Higashino, Kotaro Yoshimura
    DERMATOLOGIC SURGERY 37 (5) 605 - 610 1076-0512 2011/05 [Refereed][Not invited]
     
    BACKGROUND Postinflammatory hyperpigmentation (PIH) is the most common skin complication in Asians after invasive cosmetic treatments. OBJECTIVE To determine whether oral tranexamic acid (TA) reduces the incidence of PIH after Q-switched ruby laser (QSRL) treatment. METHODS AND MATERIALS Thirty-two Japanese women underwent QSRL treatment for senile lentigines on the face. They were randomly divided into two groups that did (n=15) and did not (n=17) receive oral TA treatment (750 mg/d) for the first 4 weeks after QSRL treatment. Nineteen participants had melasma-like maculae at baseline. Clinical and colorimetric assessments were performed at baseline and 2 and 4 weeks later. RESULTS Pigmentation was effectively treated using QSRL at 2 weeks, but PIH was frequently seen at 4 weeks. There was no significant difference in the incidence of PIH between participants who received oral TA and those who did not. The presence of melasma did not influence the effectiveness of the treatment. CONCLUSION Although oral TA has been reported to have depigmentation effects, it may not be effective for preventing PIH after QSRL. Considering the dosage and duration of treatment, an optimal protocol may be needed to induce the efficacy of this treatment to achieve the PIH-preventing effect of oral TA. The authors have indicated no significant interest with commercial supporters.
  • Naoki Kobayashi, Kohjiro Ueki, Yukiko Okazaki, Aya Iwane, Naoto Kubota, Mitsuru Ohsugi, Motoharu Awazawa, Masatoshi Kobayashi, Takayoshi Sasako, Kazuma Kaneko, Miho Suzuki, Yoshitaka Nishikawa, Kazuo Hara, Kotaro Yoshimura, Isao Koshima, Susumu Goyama, Koji Murakami, Junko Sasaki, Ryozo Nagai, Mineo Kurokawa, Takehiko Sasaki, Takashi Kadowaki
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108 (14) 5753 - 5758 0027-8424 2011/04 [Refereed][Not invited]
     
    Obesity and insulin resistance, the key features of metabolic syndrome, are closely associated with a state of chronic, low-grade inflammation characterized by abnormal macrophage infiltration into adipose tissues. Although it has been reported that chemokines promote leukocyte migration by activating class IB phosphoinositide-3 kinase (PI3K gamma) in inflammatory states, little is known about the role of PI3K gamma in obesity-induced macrophage infiltration into tissues, systemic inflammation, and the development of insulin resistance. In the present study, we used murine models of both diet-induced and genetically induced obesity to examine the role of PI3K gamma in the accumulation of tissue macrophages and the development of obesity-induced insulin resistance. Mice lacking p110 gamma (Pik3cg(-/-)), the catalytic subunit of PI3K gamma, exhibited improved systemic insulin sensitivity with enhanced insulin signaling in the tissues of obese animals. In adipose tissues and livers of obese Pik3cg(-/-) mice, the numbers of infiltrated proinflammatory macrophages were markedly reduced, leading to suppression of inflammatory reactions in these tissues. Furthermore, bone marrow-specific deletion and pharmacological blockade of PI3K gamma also ameliorated obesity-induced macrophage infiltration and insulin resistance. These data suggest that PI3K gamma plays a crucial role in the development of both obesity-induced inflammation and systemic insulin resistance and that PI3K gamma can be a therapeutic target for type 2 diabetes.
  • Xiaohong Zhang, Masako Hirai, Susana Cantero, Rodica Ciubotariu, Ludy Dobrila, Allen Hirsh, Koichi Igura, Hitoshi Satoh, Izuru Yokomi, Toshihide Nishimura, Satoru Yamaguchi, Kotaro Yoshimura, Pablo Rubinstein, Tsuneo A. Takahashi
    JOURNAL OF CELLULAR BIOCHEMISTRY 112 (4) 1206 - 1218 0730-2312 2011/04 [Refereed][Not invited]
     
    Human umbilical cord blood (CB) is a potential source for mesenchymal stem cells (MSC) capable of forming specific tissues, for example, bone, cartilage, or muscle. However, difficulty isolating MSC from CB (CB-MSC) has impeded their clinical application. Using more than 450 CB units donated to two public CB banks, we found that successful cell recovery fits a hyper-exponential function of time since birth with very high fidelity. Additionally, significant improvement in the isolation of CB-MSC was achieved by selecting cord blood units having a volume >= 90 ml and time <= 2 h after donor's birth. This resulted in 90% success in isolation of CB-MSC by density gradient purification and without a requirement for immunoaffinity methods as previously reported. Using MSC isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC) as reference controls, we observed that CB-MSC exhibited a higher proliferation rate and expanded to the order of the 1 x 10(9) cells required for cell therapies. CB-MSC showed karyotype stability after prolonged expansion. Functionally, CB-MSC could be more readily induced to differentiate into chondrocytes than could BM-MSC and AT-MSC. CB-MSC showed immunosuppressive activity equal to that of BM-MSC and AT-MSC. Collectively, our data indicate that viable CB-MSC could be obtained consistently and that CB should be reconsidered as a practical source of MSC for cell therapy and regenerative medicine using the well established CB banking system. J. Cell. Biochem. 112: 1206-1218, 2011. (C) 2011 Wiley-Liss, Inc.
  • Gregorio Chazenbalk, Cristina Bertolotto, Saleh Heneidi, Medet Jumabay, Bradley Trivax, Joel Aronowitz, Kotaro Yoshimura, Charles F. Simmons, Daniel A. Dumesic, Ricardo Azziz
    PLOS ONE 6 (3) e17834  1932-6203 2011/03 [Refereed][Not invited]
     
    Introduction: Previous studies highlight a complex relationship between lineage and phenotype for adipose tissue macrophages (ATMs), adipose stem cells (ASCs), and adipocytes, suggesting a high degree of plasticity of these cells. In the present study, using a novel co-culture system, we further characterized the interaction between ATMs, ASCs and adipocytes. Research Design and Methods: Human adipocytes and the stromal vascular fraction containing ATMs and ASCs were isolated from human adipose tissue and co-cultured for 24 hours. FACS was used to characterize ATMs and ASCs before and after co-culture. Preadipocytes generated after co-culture were characterized by immunostaining for DLK (preadipocytes), CD14 and CD68 (ATMs), CD34 (ASCs), and Nile Red staining for lipid drops. qRT-PCR was used to quantify adipogenic markers such as C/EBP alpha and PPAR gamma. A novel fluorescent nanobead lineage tracing method was utilized before co-culture where fluorescent nanobeads were internalized by CD68 (+) ATMs. Results: Co-culture of adipocytes with ATMs and ASCs increased the formation of new preadipocytes, thereby increasing lipid accumulation and C/EBP alpha and PPAR gamma gene expression. Preadipocytes originating after co-culture were positive for markers of preadipocytes, ATMs and ASCs. Moreover, fluorescent nanobeads were internalized by ATMs before co-culture and the new preadipocytes formed after co-culture also contained fluorescent nanobeads, suggesting that new preadipocytes originated in part from ATMs. The formation of CD34(+)/CD68(+)/DLK(+) cell spheres supported the interaction of ATMs, ASCs and preadipocytes. Conclusions: Cross-talk between adipocytes, ATMs and ASCs promotes preadipocyte formation. The regulation of this novel adipogenic pathway involves differentiation of ATMs to preadipocytes. The presence of CD34(+)/CD68(+)/DLK(+) cells grouped in spheres suggest that paracrine interactions between these cell types plays an important role in the generation and proliferation of new preadipocytes. This phenomenon may reflect the in vivo plasticity of adipose tissue in which ATMs play an additional role during inflammation and other disease states. Understanding this novel pathway could influence adipogenesis, leading to new treatments for obesity, inflammation, and type 2 diabetes.
  • Masakazu Kurita, Daisuke Matsumoto, Harunosuke Kato, Jun Araki, Takuya Higashino, Takashi Fujino, Katsuya Takasu, Kotaro Yoshimura
    AESTHETIC SURGERY JOURNAL 31 (3) 347 - 351 1090-820X 2011/03 [Refereed][Not invited]
     
    Background: Thread lifting has become popular as a minimally-invasive suspension procedure, but there is little basic and clinical evidence in the literature on the long-term effects. Objectives: The authors investigate the effects of two types of lifting threads in a rat model over the course of seven months. Methods: The dorsal skin of 18 Wistar rats was implanted with a 20-mm fragment of one of three types of thread: nonabsorbable monofilament cog, pure gold (24 karat) with no cog, and pure gold-coated cog. Six rats were in each group. Tissue samples were harvested and histologically evaluated at one, three, and seven months. Results: Histological assessment indicated (1) acute tissue reactions to the regular cog thread involving myofibroblasts and (2) delayed tissue reactions to the pure gold thread involving giant cells. The gold-coated cog thread showed a combination of the histological reactions associated with the cog thread and the pure gold thread, including faint early reactions, strong delayed reactions, and long-lasting capsule formation. Notably, the gold coating gradually came loose from the thread surface, suggesting that the release of tiny gold particles may promote longer-lasting tissue reactions. Conclusions: The combination of cog structure and pure gold coating was evaluated for the first time in this study and results suggest that the gold-coated cog thread has clinical potential.
  • Gimble JM, Bunnell BA, Casteilla L, Jung JS, Yoshimura K
    Stem cells international 2010 604713  1687-966X 2011/02 [Refereed][Not invited]
  • Toshitsugu Hirohi, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 127 (1) 396 - 406 0032-1052 2011/01 [Refereed][Not invited]
     
    Background: Although double eyelid plasty, levator aponeurotic surgery, and epicanthoplasty are well-accepted cosmetic treatments for Asian eyes, some patients are incompletely satisfied with the outcomes and request further surgery. Although lower eyelid descent is generally recognized as a symptom of aging or a complication after blepharoplasty, the authors propose a perceptional change: a lowering the lower eyelid procedure to vertically enlarge the palpebral aperture in selected Asian patients. Methods: A total of 125 Japanese patients underwent the lowering the lower eyelid procedure between 2005 and 2009. The main indications were patients with vertically narrow palpebral aperture or an up-slanting appearance. The lowering the lower eyelid procedure is performed by a combination of the removal of approximately 4 to 6 mm of the subciliary skin (usually the lateral one-third to two-thirds of the lower eyelids) and static shortening of the lower eyelid retractors (posterior lamella) through a transconjunctival approach. The middle lamella was not touched during the procedure. Results: The up-slanting lower eyelid margin was lowered and the lateral part of the palpebral aperture was enlarged by the procedure in all cases. Cosmetic outcomes were encouraging and satisfying to most patients. Three complications occurred (2.4 percent): lagophthalmos in one patient (0.8 percent) and entropion in two patients (1.6 percent). These minor complications resolved within 1 month. Eight revision operations were required for undercorrection. Conclusions: The lowering the lower eyelid procedure offers Asian patients desiring large oval eyes a novel surgical option. The procedure proved to be a reliable and consistent technique that provided satisfactory results in carefully selected patients. (Plast. Reconstr. Surg. 127: 396, 2011.)
  • Hirotaka Suga, Hitomi Eto, Noriyuki Aoi, Harunosuke Kato, Jun Araki, Kentaro Doi, Takuya Higashino, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 126 (6) 1911 - 1923 0032-1052 2010/12 [Refereed][Not invited]
     
    Background: Following various types of plastic surgery, such as adipose grafting and flap elevation, adipose tissue undergoes ischemia, leading to hypoxia and nutrient depletion. However, few studies have examined ischemic and/or hypoxic changes in adipose tissue. Methods: The authors established surgically induced ischemia models by severing blood vessels supplying the inguinal fat pads in mice. The partial pressure of oxygen in adipose tissue was measured with an oxygen monitor, and ischemic changes were analyzed by whole-mount staining, immunohistochemistry, flow cytometry, and Western blotting. The authors also examined cell survival under a hypoxic condition in vitro. Results: Models for three degrees (mild, intermediate, and severe) of ischemia showed approximately 75, 55, and 20 percent of the partial pressure of oxygen level in normal adipose tissue (50.5 +/- 1.3 mm Hg), respectively. Adipose tissue atrophy with substantial fibrosis on day 28 was seen, depending on the severity of ischemia. Intermediate and severe ischemia induced elevated expression of hypoxia-inducible factor 1 alpha and fibroblast growth factor 2 on day 1 and degenerative changes (i.e., apoptosis, necrosis, and macrophage infiltration and phagocytosis) in adipose tissue. Dead cells included adipocytes, vascular endothelial cells, and blood-derived cells, but not adipose-derived stem/progenitor cells. Subsequent to degenerative changes, regenerative changes were seen, including angiogenesis, adipogenesis, and proliferation of cells (adipose-derived stem/progenitor cells, vascular endothelial cells, and blood cells). The authors found that, in vitro, the experimentally differentiated adipocytes underwent apoptosis and/or necrosis under severe hypoxia, but adipose-derived stem/progenitor cells remained viable. Conclusions: Severe ischemia/hypoxia induces degenerative changes in adipose tissue and subsequent adaptive tissue remodeling. Adipocytes die easily under ischemic conditions, whereas adipose-derived stem/progenitor cells are activated and contribute to adipose tissue repair. (Plast. Reconstr. Surg. 126: 1911, 2010.)
  • Harunosuke Kato, Rie Yoshida, Katsuhiko Tsukamoto, Hirotaka Suga, Hitomi Eto, Takuya Higashino, Jun Araki, Tsutomu Ogata, Kotaro Yoshimura
    INTERNATIONAL JOURNAL OF DERMATOLOGY 49 (10) 1146 - 1151 0011-9059 2010/10 [Refereed][Not invited]
     
    P>Five familial cases exhibited ephelides-like multiple lentigines, and we examined three of them, a mother and two sons. All three patients presented with small dark-brown maculae on the face and neck and electrocardiographic abnormalities. These findings sufficed to fulfill the criteria for LEOPARD syndrome (multiple lentigines syndrome), although they lacked five of seven major clinical features. However, the family members presented with a webbed neck and pectus excavatum, which are more frequently seen in Turner or Noonan syndrome. Histological examination of the lentigines revealed slightly elongated rete ridges, a hyperpigmented basal layer, and melanophages in the papillary dermis. Direct sequencing of the patients' genomic DNA revealed that all three had a consistent missense mutation [c.1403C > T (p.T468M)] in the PTPN11 gene, confirming LEOPARD syndrome with an atypical phenotype. It was suggested that LEOPARD syndrome shows a diverse phenotype but its diagnosis can be verified by mutation analysis.
  • Toshitsugu Hirohi, Kotaro Yoshimura
    JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY 63 (8) 1251 - 1259 1748-6815 2010/08 [Refereed][Not invited]
     
    Background: In Asian countries, many patients with a prominent mandibular angle desire its correction, because they consider it to be an unappealing feature. Reduction mandibuloplasty has been frequently performed through the intraoral approach, but an invisible mandibular angle forces the surgeon to perform blind ostectomy. In addition, the limited mobility of the oscillating saw leads to semi-vertical ostectomy, and leaves unnatural mandibular contours, such as loss of the mandibular angle. Methods: To overcome the drawbacks of conventional procedures, we performed en bloc mandibular corpus-angle ostectomy using a contra-angle handpiece and subsequent corticectomy in 519 patients with prominent mandibular angles. A retractor with an endoscope was supportively used in 190 patients. A pre- and postoperative cephalogram was taken in 86 patients, and the gonial angle (GA) and the mandibular plane angle to the Frankfort horizontal plane (MPA) were measured. Results: The majority of patients were satisfied with the aesthetic results. GA and MPA were increased by approximately 10 degrees. GA was successfully improved to within the pre-set desired range in 84.5% and 60.0% of the female and male patients, respectively. The overall complication rate was 4.0%; all of these were minor complications, and no major complication such as malfracture or facial nerve injury was seen. Conclusions: Our new technique allows surgeons to perform accurate, safe and reproducible ostectomies and to reshape prominent angles to more natural-looking ones with smooth ostectomised borders. (C) 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
  • Harunosuke Kato, Hirotaka Suga, Hitomi Eto, Jun Araki, Noriyuki Aoi, Kentaro Doi, Takuya Iida, Yasuhiko Tabata, Kotaro Yoshimura
    TISSUE ENGINEERING PART A 16 (6) 2029 - 2040 1937-3341 2010/06 [Refereed][Not invited]
     
    Various kinds of tissue expansion have been performed clinically with internal devices, but external expansion has not been previously investigated. We applied continuous external force on skin tissue in a mouse model. Four weeks of external suspension caused enlargement of the subcutaneous tissue, particularly adipose tissue, although the enlargement was reversible. We found that the enlarged tissue volume could be adequately sustained with controlled release of basic fibroblast growth factor (bFGF), administered at the time the device was removed. Ki67+ proliferating cells, perilipin+ small adipocytes, lectin+ capillaries, and glycerol-3-phosphate dehydrogenase activity in the tissue increased during the expansion process, indicating dynamic adipose remodeling with adipogenesis and angiogenesis. Histological analyses revealed that vessels had elongated in the direction of the external force. Adipose-resident progenitor cells (adipose-derived stem/stromal cells) were the primary proliferating cell population involved in the remodeling process, particularly in the superficial layer. Treatment with bFGF did not enhance the small adipocyte number, but promoted angiogenesis; this mechanism may contribute to the preservation of enlarged tissue. Our results suggested that external tissue suspension induced adipose tissue enlargement by activating resident progenitor cells and that this external suspension approach, combined with controlled release of bFGF, has therapeutic potential for soft tissue engineering.
  • Kotaro Yoshimura, Yuko Asano, Noriyuki Aoi, Masakazu Kurita, Yoshio Oshima, Katsujiro Sato, Keita Inoue, Hirotaka Suga, Hitomi Eto, Harunosuke Kato, Kiyonori Harii
    BREAST JOURNAL 16 (2) 169 - 175 1075-122X 2010/03 [Refereed][Not invited]
     
    Breast enhancement with artificial implants is one of the most frequently performed cosmetic surgeries but is associated with various complications, such as capsular contracture, that lead to implant removal or replacement at a relatively high rate. For replacement, we used transplantation of progenitor-supplemented adipose tissue (cell-assisted lipotransfer; CAL) in 15 patients. The stromal vascular fraction containing adipose tissue progenitor cells obtained from liposuction aspirates was used to enrich for progenitor cells in the graft. Overall, clinical results were very satisfactory, and no major abnormalities were seen on magnetic resonance imaging or mammogram after 12 months. Postoperative atrophy of injected fat was minimal and did not change substantially after 2 months. Surviving fat volume at 12 months was 155 +/- 50 mL (Right; mean +/- SD) and 143 +/- 80 mL (Left) following lipoinjection from an initial mean of 264 mL. These preliminary results suggest that CAL is a suitable methodology for the replacement of breast implants.
  • Hirotaka Suga, Jun Araki, Noriyuki Aoi, Harunosuke Kato, Takuya Higashino, Kotaro Yoshimura
    JOURNAL OF CUTANEOUS PATHOLOGY 36 (12) 1293 - 1298 0303-6987 2009/12 [Refereed][Not invited]
     
    Suga H, Araki J, Aoi N, Kato H, Higashino T, Yoshimura K. Adipose tissue remodeling in lipedema: adipocyte death and concurrent regeneration.
  • Keita Inoue, Noriyuki Aoi, Yuji Yamauchi, Takahiro Sato, Hirotaka Suga, Hitomi Eto, Harunosuke Kato, Yasuhiko Tabata, Kotaro Yoshimura
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 13 (11-12) 4643 - 4656 1582-1838 2009/11 [Refereed][Not invited]
     
    Dermal papilla cells (DPCs) in the mammalian hair follicle have been shown to develop hair follicles through epithelial-mesenchymal interactions. A cell therapy to regenerate human hair is theoretically possible by expanding autologous human DPCs (hDPCs) and transplanting them into bald skin, though much remains to be overcome before clinical success. In this study, we compared gene signatures of hDPCs at different passages and human dermal fibroblasts, and found transforming growth factor (TGF)-beta(2) to be highly expressed in cultured hDPCs. Keratinocyte conditioned medium, which is known to help preserve the hair-inducing capacity of hDPCs, up-regulated TGF-beta(2) expression of hDPCs and also enhanced their alkaline phosphatase (ALP) activity, a known index for hair-inductive capacity. Through screening of components secreted from keratinocytes, the vitamin D-3 analogue was found to promote TGF-beta(2) expression and ALP activity of hDPCs. In animal hair folliculogenesis models using rat epidermis and expanded hDPCs, inhibition of TGF-beta(2) signalling at the ligand or receptor level significantly impaired hair folliculogenesis and maturation. These results suggest an important role for TGF-beta(2) in hair follicle morphogenesis and provide insights into the establishment of future cell therapies for hair regrowth by transplanting expanded DPCs.
  • Hitomi Eto, Hirotaka Suga, Daisuke Matsumoto, Keita Inoue, Noriyuki Aoi, Harunosuke Kato, Jun Araki, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 124 (4) 1087 - 1097 0032-1052 2009/10 [Refereed][Not invited]
     
    Background: Adipose tissue is an easily accessible tissue for use as a soft-tissue filler and source of adult multipotent cells, called adipose-derived stem/stromal/progenitor cells. However, many aspects of its anatomy remain unclear because of the fragile structure of adipocytes and adipose tissue. Methods: Aspirated (n = 15) or intact (n = 9) subcutaneous adipose tissue samples were evaluated by (1) whole-mount histology with triple-fluorescence staining for three-dimensional visualization of living adipose tissue, (2) glycerol-3-phosphate dehydrogenase assay, (3) multicolor flow cytometry (CD34, CD31, and CD45), and (4) adherent cell culture of stromal vascular fraction cells for viable adipose-derived stromal cell yield. Results: Whole-mount histology revealed the presence of a capillary network running alongside adipocytes, which was partly disrupted in aspirated adipose tissues. Aspirated adipose tissue also lacked large vasculature structures and showed significantly larger numbers of small lipid droplets (ruptured adipocytes) (p = 0.00016) and dead cells (p = 0.0038) compared with excised adipose tissue. Adipocyte number was less than 20 percent of total cellularity, and vasculature-associated cells, including endothelial cells and adipose-derived stromal cells, constituted over one-half of total cells in both intact and aspirated adipose tissue. Glycerol-3-phosphate dehydrogenase assay suggested that greater than 30 percent and 5 percent of adipocytes were ruptured in aspirated and excised adipose tissue, respectively (p = 0.032). Multicolor flow cytometric analysis indicated a much higher percentage of blood-derived cells (CD45(+)) contaminated in aspirated adipose tissue (p = 0.0038), and adipose-derived stromal cell yield in aspirated adipose tissue was approximately one-half that in excised tissue (p = 0.011). Conclusion: The authors' results indicate the differential structure and cellular composition of the two tissues, and significant tissue damage and progenitor yield deficiency in aspirated adipose tissue. (Plast. Reconstr. Surg. 124: 1087, 2009.)
  • Hirotaka Suga, Daisuke Matsumoto, Hitomi Eto, Keita Inoue, Noriyuki Aoi, Harunosuke Kato, Jun Araki, Kotaro Yoshimura
    STEM CELLS AND DEVELOPMENT 18 (8) 1201 - 1209 1547-3287 2009/10 [Refereed][Not invited]
     
    CD34 is frequently used as a marker of adipose-derived stem/stromal/progenitor cells (ASCs). However, CD34 expression in human ASCs (hASCs) decreases over time in culture, and the implications of this remain unclear. In this study, we sorted shortly-cultured hASCs into CD34+ and CD34- fractions and compared their biological functions. Results indicated that CD34+ hASCs were more proliferative and had a greater ability to form colonies. In contrast, CD34-cells showed a greater ability for differentiation into adipogenic and osteogenic lineages. Both CD34+ and CD34-cells showed similar abilities in migration and capillary formation in response to growth factors. Expression levels of endothelial progenitor markers (Flk-1, FLT1, and Tie-2) were higher in CD34+ cells, whereas those of pericyte markers (CD146, NG2, and alpha-smooth muscle actin) were higher in CD34- cells. Both CD34+ and CD34-cells showed similar expression levels of vascular endothelial growth factor and hepatocyte growth factor, although hypoxia affected the expression levels. Together these results suggest that CD34 expression in hASCs may correlate with replicative capacity, differentiation potentials, expression profiles of angiogenesis-related genes, and immaturity or stemness of the cells. Loss of CD34 expression may be related to the physiological process of commitment and/or differentiation from immature status into specific lineages such as adipose, bone, or smooth muscle.
  • Noriyuki Aoi, Kotaro Yoshimura, Takafumi Kadono, Gojiro Nakagami, Shinji Iizuka, Takuya Higashino, Jun Araki, Isao Koshima, Hiromi Sanada
    Plastic and reconstructive surgery 124 (2) 540 - 50 0032-1052 2009/08 [Refereed][Not invited]
     
    BACKGROUND: The concept of deep tissue injury under intact skin helps us understand the pathogenesis of pressure ulcers, but the best method for detecting and evaluating deep tissue injury remains to be established. METHODS: Intermediate-frequency (10-MHz) ultrasonography was performed to evaluate deep tissue injury. The authors analyzed 12 patients (nine male patients and three female patients aged 16 to 92 years) who showed deep tissue injury-related abnormal findings on ultrasonography at the first examination and were followed up until the pressure ulcer reached a final stage. RESULTS: The stage of ulcer worsened in six of 12 cases compared with baseline, and healed in the remaining six patients. The authors recognized four types of abnormal signs unique to deep tissue damage in ultrasonography: unclear layered structure, hypoechoic lesion, discontinuous fascia, and heterogeneous hypoechoic area. Unclear layered structure, hypoechoic lesion, discontinuous fascia, and heterogeneous hypoechoic area were detected at the first examination in 12, 10, seven, and five patients, respectively. Unclear layered structure and hypoechoic lesion were more commonly seen in pressure ulcers in deep tissue injury than the other features, but the follow-up study suggested that discontinuous fascia and heterogeneous hypoechoic area are more reliable predictors of future progression of pressure ulcers. CONCLUSIONS: The use of intermediate-frequency ultrasound reliably identified deep tissue injury and was believed to contribute to prevention and treatment of pressure-related ulcers. The results suggest that specific ultrasonographic characteristics may predict which pressure ulcers will progress.
  • Satoshi Nishimura, Ichiro Manabe, Mika Nagasaki, Koji Eto, Hiroshi Yamashita, Mitsuru Ohsugi, Makoto Otsu, Kazuo Hara, Kohjiro Ueki, Seiryo Sugiura, Kotaro Yoshimura, Takashi Kadowaki, Ryozo Nagai
    NATURE MEDICINE 15 (8) 914 - U116 1078-8956 2009/08 [Refereed][Not invited]
     
    Inflammation is increasingly regarded as a key process underlying metabolic diseases in obese individuals. In particular, obese adipose tissue shows features characteristic of active local inflammation. At present, however, little is known about the sequence of events that comprises the inflammatory cascade or the mechanism by which inflammation develops. We found that large numbers of CD8(+) effector T cells infiltrated obese epididymal adipose tissue in mice fed a high-fat diet, whereas the numbers of CD4(+) helper and regulatory T cells were diminished. The infiltration by CD8(+) T cells preceded the accumulation of macrophages, and immunological and genetic depletion of CD8(+) T cells lowered macrophage infiltration and adipose tissue inflammation and ameliorated systemic insulin resistance. Conversely, adoptive transfer of CD8(+) T cells to CD8-deficient mice aggravated adipose inflammation. Coculture and other in vitro experiments revealed a vicious cycle of interactions between CD8(+) T cells, macrophages and adipose tissue. Our findings suggest that obese adipose tissue activates CD8(+) T cells, which, in turn, promote the recruitment and activation of macrophages in this tissue. These results support the notion that CD8(+) T cells have an essential role in the initiation and propagation of adipose inflammation.
  • Keita Inoue, Noriyuki Aoi, Takahiro Sato, Yuji Yamauchi, Hirotaka Suga, Hitomi Eto, Harunosuke Kato, Jun Araki, Kotaro Yoshimura
    LABORATORY INVESTIGATION 89 (8) 844 - 856 0023-6837 2009/08 [Refereed][Not invited]
     
    Several putative biomarkers have been suggested for identifying murine follicular stem cells; however, human hair follicles have a different pattern of biomarker expression, and follicular stem cell isolation methods have not been established. To isolate a stem cell population applicable to clinical settings, we conducted a comprehensive survey of the expression of stem-cell-associated (K15, CD200, CD34, and CD271) and other biomarkers (K1, K14, CD29, and CD49f) in immunohistological sections of the human epidermis and follicular outer root sheath (ORS). We also examined freshly isolated and cultured epidermal or follicular cells with single- and multicolor flow cytometry or immunocytochemistry. After sorting cells by CD200, CD34, and forward scatter (FSC) values (cell size), colony-forming assays were performed. We found that biomarkers were differentially expressed in the epidermis and ORS. Basal bulge cells were mainly K15(+)CD200(+)CD34(-)CD271(-), and suprabasal cells were K15(-)CD200(+)CD34(-)CD271(-). We categorized follicular cells into nine subpopulations according to biomarker expression profiles. The CD200(+)CD34(-) bulge cells had much higher colony-forming abilities than the CD34(+) population, and were divided into two subpopulations: a CD200(+)CD34(-)FSC(high) (K15-rich, basal) and a CD200(+)CD34-FSC(low) (K15-poor, suprabasal) population. The former formed fewer but larger-sized colonies than the latter. Follicular epithelial cell cultivation resulted in loss of K15, CD200, CD34, and CD271 expression, but maintenance of K14, CD29, and CD49f expression. We found that the bulge contained two populations with different localizations, cell sizes, and colony-forming abilities. We showed that K15, CD200, CD34, and CD271 were useful biomarkers for characterizing freshly isolated human follicular epithelial cells in diverse stages of differentiation. Laboratory Investigation (2009) 89, 844-856; doi:10.1038/labinvest.2009.48; published online 8 June 2009
  • Keita Inoue, Harunosuke Kato, Takahiro Sato, Aki Osada, Noriyuki Aoi, Hirotaka Suga, Hitomi Eto, Koichi Gonda, Kotaro Yoshimura
    CELLS TISSUES ORGANS 190 (2) 102 - 110 1422-6405 2009 [Refereed][Not invited]
     
    Background: The dermal papilla (DP) interacts with epithelial cells for folliculogenesis. For translational research on cell therapies for hair regrowth with cultured human DP cells (hDPCs), a model to evaluate the capacity of hDPCs to induce hair formation is inevitable. Methods: Chamber models were constructed by transplanting 4 different combinations of mouse or human epithelial and mesenchymal cells into a silicone chamber implanted onto the back of nude mice. In parallel, 3 types of sandwich constructs were created by inserting hDPCs or human DP tissue between the epidermis and dermis of isolated rat footpad skin or human facial skin, and subcutaneously transplanting the constructs into the back of nude mice. Four to six weeks later, skin sections of each model were histologically examined. Results: Folliculoneogenesis was detected in both chamber and sandwich models, although the induction rate and maturity of the hair follicles varied among cell combination subgroups in each model. The difference in hair induction rate was not statistically significant between 2 representative chamber and sandwich subgroups using cultured hDPCs. The sandwich model, however, required fewer hDPCs, did not require human keratinocytes, and exhibited a higher rate of successful sample collection. Conclusions: Although there is no significant difference in hair induction rate, the sandwich model using cultured hDPCs and the rat sole skin is more feasible than the chamber model using human cultured keratinocytes and hDPCs as a tool to evaluate the hair-inducing capacity of cultured hDPCs. Copyright (c) 2008 S. Karger AG, Basel
  • Hirotaka Suga, Hitomi Eto, Tomokuni Shigeura, Keita Inoue, Noriyuki Aoi, Harunosuke Kato, Satoshi Nishimura, Ichiro Manabe, Koichi Gonda, Kotaro Yoshimura
    STEM CELLS 27 (1) 238 - 249 1066-5099 2009 [Refereed][Not invited]
     
    Adipose-derived stem/stromal cells (ASCs) not only function as tissue-specific progenitor cells but also are multipotent and secrete angiogenic growth factors, such as hepatocyte growth factor (HGF), under certain circumstances. However, the biological role and regulatory mechanism of this secretion have not been well studied. We focused on the role of ASCs in the process of adipose tissue injury and repair and found that among injury-associated growth factors, fibroblast growth factor-2 (FGF-2) strongly promoted ASC proliferation and HGF secretion through a c-Jun N-terminal kinase (JNK) signaling pathway. In a mouse model of ischemia-reperfusion injury of adipose tissue, regenerative changes following necrotic and apoptotic changes were seen for 2 weeks. Acute release of FGF-2 by injured adipose tissue was followed by upregulation of HGF. During the adipose tissue remodeling process, adipose-derived 5-bromo-2-deoxyuridine-positive cells were shown to be ASCs (CD31-CD34+). Inhibition of JNK signaling inhibited the activation of ASCs and delayed the remodeling process. In addition, inhibition of FGF-2 or JNK signaling prevented postinjury upregulation of HGF and led to increased fibrogenesis in the injured adipose tissue. Increased fibrogenesis also followed the administration of a neutralizing antibody against HGF. FGF-2 released from injured tissue acts through a JNK signaling pathway to stimulate ASCs to proliferate and secrete HGF, contributing to the regeneration of adipose tissue and suppression of fibrogenesis after injury. This study revealed a functional role for ASCs in the response to injury and provides new insight into the therapeutic potential of ASCs. STEM CELLS 2009; 27: 238-249
  • Masakazu Kurita, Emiko Aiba-Kojima, Tomokuni Shigeura, Daisuke Matsumoto, Hirotaka Suga, Keita Inoue, Hitomi Eto, Harunosuke Kato, Noriyuki Aoi, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 122 (2) 438 - 448 0032-1052 2008/08 [Refereed][Not invited]
     
    Background: To avoid potential risks of animal-derived products such as viral transmission and immunologic reactions, usefulness of human-derived products in manipulation of cells for cell-based therapies has been investigated but has not yet been completely clarified. Methods: Three types of human sera-serum from whole blood, serum from platelet-rich plasma, and serum from platelet-poor plasma-were prepared from blood samples obtained from the same four volunteers. The authors investigated the biochemical profiles of the three serum preparations as well as their potential as culture additives using three types of human cells: dermal fibroblasts, adipose-derived stem/stromal cells, and umbilical vein endothelial cells. Results: Platelet counts differed among serum from whole blood (100 percent), platelet-rich plasma (75.1 percent), and platelet-poor plasma (12.6 percent), resulting in differential concentrations of platelet-derived growth factor and epidermal growth factor, although other biochemical values such as total protein and albumin were similar. Serum from whole blood and platelet-rich plasma highly enhanced proliferation of dermal fibroblasts compared with the effects of serum from platelet-poor plasma, but no differences in proliferative efficacy were observed in cultures of adipose-derived stem/stromal cells and vascular endothelial cells. Conclusions: Serum from platelet-rich plasma, which is less invasive to prepare than serum from whole blood, was superior to serum from platelet-poor plasma as a substitute for animal-derived serum in culture expansion of dermal fibroblasts. Although autologous or human-derived serum preparations may be of great use in cell-based therapies, this usefulness strongly depends on the target cell species and the purpose of the cell culture.
  • Hirotaka Suga, Daisuke Matsumoto, Keita Inoue, Tomokuni Shigeura, Hitomi Eto, Noriyuki Aoi, Harunosuke Kato, Hiroaki Abe, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 122 (1) 103 - 113 0032-1052 2008/07 [Refereed][Not invited]
     
    Background: A reliable method with which to assay viability and number of adipocytes and other cellular components in adipose tissue remains to be established. Methods: The authors assessed cell viability and number obtained from 1 g of suctioned adipose tissue and respective layers (the top, middle, and bottom layers) before and after digestion and centrifugation, using cell staining with Hoechst 33342 and propidium iodide and the 2,3-bis(2-methoxy-4-vitro-5-sulfophenyl)-5-[(phenyl-amino)carbonyl]-2H-tetrazoliumhydroxide (XTT) and glycerol-3-phosphate dehydrogenase assays (n = 10). The correlation between the number of prepared cells (adipocytes, adipose stromal cells, and white blood cells) and the resulting values from the XTT and glycerol-3-phosphate dehydrogenase assays was also examined (n = 5). The cell composition of the stromal vascular fraction isolated from the same adipose tissue was determined by multicolor flow cytometry (n = 5). Results: Hoechst 33342 and propidium iodide staining allowed distinguishing of viable adipocytes from lipid droplets, dead adipocytes, and cells other than adipocytes. The authors obtained 6.9 X 10(5) nonruptured adipocytes from 1 g of suctioned adipose tissue; 30 percent of the original adipocytes appeared to have been ruptured. Both the XTT and glycerol-3-phosphate dehydrogenase assays provided good correlations between the number of viable adipocytes and resulting values, but only the glycerol-3-phosphate dehydrogenase assay was strictly specific for adipocytes. The ratio of adipose stromal cells to adipocytes was found to be much larger than previously described. Conclusion: Single use or a combination of the viability assays used in this study can appropriately determine the number of adipocytes and other cells, although it remains difficult to assess original cells directly without tissue dissociation.
  • Kotaro Yoshimura, Noriyuki Aoi, Hirotaka Suga, Keita Inoue, Hitomi Eto, Katsujiro Sato, Masakazu Kurita, Kiyonori Harii, Toshitsugu Hirohi
    TRANSPLANTATION 85 (12) 1868 - 1869 0041-1337 2008/06 [Refereed][Not invited]
  • Masakazu Kurita, Daisuke Matsumoto, Tomokuni Shigeura, Katsujiro Sato, Koichi Gonda, Kiyonori Harii, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 121 (3) 1033 - 1041 0032-1052 2008/03 [Refereed][Not invited]
     
    Background: Although injective autologous fat transplantation is one of the most attractive options for soft-tissue augmentation, problems such as unpredictability and fibrosis resulting from fat necrosis limit its universal acceptance. Centrifugation is one of most common methods for overcoming these difficulties. This study was performed to investigate quantitatively the effects of centrifugation on liposuction aspirates to optimize centrifugal conditions for fat transplantation and isolation of adipose-derived stem cells. Methods: Liposuction aspirates, obtained from eight healthy female donors, were either not centrifuged or centrifuged at 400, 700, 1200, 3000, or 4200 g for 3 minutes. The volumes of the oil, adipose, and fluid portions and numbers of blood cells and adipose-derived cells in each portion were examined. The processed adipose tissues (1 ml) were injected into athymic mice, and grafts were harvested and weighed at 4 weeks. Morphologic alterations were observed using light and scanning electron microscopy. Results: Centrifugation concentrated adipose tissues and adipose-derived stem cells in the adipose portion and partly removed red blood cells from the adipose portion. Centrifugation at more than 3000 g significantly damaged adipose-derived stem cells. Centrifugation enhanced graft take per 1 ml centrifuged adipose but reduced calculated graft take per 1 ml adipose before centrifugation. Conclusions: Excessive centrifugation can destroy adipocytes and adipose-derived stem cells, but appropriate centrifugation concentrates them, resulting in enhanced graft take. The authors tentatively recommend 1200 g as an optimized centrifugal force for obtaining good short- and long-term results in adipose transplantation.
  • Nagase T, Matsumoto D, Nagase M, Yoshimura K, Shigeura T, Inoue M, Hasegawa M, Yamagishi M, Machida M
    The Journal of craniofacial surgery 18 49 - 53; discussion 60 1049-2275 2007/01 [Refereed][Not invited]
  • Katsujiro Sato, Daisuke Matsumoto, Fumiko Iizuka, Emiko Aiba-Kojima, Asami Watanabe-Ono, Hirotaka Suga, Keita Inoue, Koichi Gonda, Kotaro Yoshimura
    AESTHETIC PLASTIC SURGERY 30 (6) 689 - 694 0364-216X 2006/12 [Refereed][Not invited]
     
    Background: Few studies have addressed anti-androgenic therapy using oral spironolactone for acne in Asians. Obtaining this race-specific information is important because Westerners and Asians respond differently to hormone therapy. This study aimed to examine the efficacy and safety of oral spironolactone used to treat acne in Asians. Methods: Spironolactone (initial dose, 200 mg/day) was administered orally to 139 Japanese patients (116 females and 23 males) with acne. Serum laboratory data, including various hormones and electrolytes, were examined for 25 of the subjects. Results: Most of the female patients who completed the 20-week regimen exhibited excellent improvement (evaluated by a photographic grading scale), although some discontinued treatment because of menstrual disturbances or other reasons. The treatment was less efficacious for the males than for the females, and because gynecomastia developed in three male patients, spironolactone treatment for males was stopped. Examination of the serum of 25 patients did not identify any toxicity associated with the treatment. Drug eruptions and edema in the lower extremities were each seen in three patients. Conclusion: Oral spironolactone is effective and safe for the treatment of acne in Asian females, and can be a good option for severe, recurring, and widespread types of the condition.
  • Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, Sato T, Aiba-Kojima E, Iizuka F, Inoue K, Suga H, Yoshimura K
    Tissue engineering 12 (12) 3375 - 3382 1076-3279 2006/12 [Refereed][Not invited]
  • Isao Koshima, Katsuyuki Urushibara, Norio Fukuda, Masayuki Ohkochi, Takashi Nagase, Koichi Gonda, Hirotaka Asato, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 118 (7) 1579 - 1584 0032-1052 2006/12 [Refereed][Not invited]
  • Takashi Nagase, Miki Nagase, Kotaro Yoshimura, Masafumi Machida, Masaaki Yamagishi
    JOURNAL OF CRANIOFACIAL SURGERY 17 (4) 736 - 744 1049-2275 2006/07 [Refereed][Not invited]
     
    Sonic hedgehog (Shh) is a well-known morphogen indispensable in facial and nervous development, and recently it has also garnered much attention as a potent angiogenic factor. We previously created an animal model of holoprosencephaly by administration of cyclopamine, a specific inhibitor of hedgehog signaling, to the mouse embryos cultured in vitro, and found several types of angiogenic defects. In this study, we focused on other angiogenic phenotypes in the same model. When cyclopamine was added for embryonic day (E) 8.0-9.5, a pair of immature dorsal aortae, which normally fuse to form the single aorta by E9.5, remained to be separated. Expressions of vascular endothelial growth factor and bone morphogenetic protein 4, putative mediators of aortic fusion, were also reduced around the aorta by blockade of Shh signaling. When cyclopamine was added for E8.5-10.5, vessels on the surface of craniofacial region (possibly external cardinal veins) were extended and malformed. These results suggest that Shh signaling is essential for some aspects of embryonic angiogenesis, and that pathophysiology of holoprosencephaly may involve, at least in part, the Shh-dependent angiogenesis.
  • K Yoshimura, T Shigeura, D Matsumoto, T Sato, Y Takaki, E Aiba-Kojima, K Sato, K Inoue, T Nagase, Koshima, I, K Gonda
    JOURNAL OF CELLULAR PHYSIOLOGY 208 (1) 64 - 76 0021-9541 2006/07 [Refereed][Not invited]
     
    Liposuction aspirates (primarily saline solution, blood, and adipose tissue fragments) separate into fatty and fluid portions. Cells isolated from the fatty portion are termed processed lipoaspirate (PLA) cells and contain adipose-derived adherent stromal cells (ASCs). Here we define cells isolated from the fluid portion of liposuction aspirates as liposuction aspirate fluid (LAF) cells. Stromal vascular fractions (SVF) were isolated separately from both portions and characterized under cultured and non-cultured conditions. A comparable number of LAF and PLA cells were freshly isolated, but fewer LAF cells were adherent. CD34(+)CD45(-) cells from fresh LAF isolates were expanded by adherent culture, suggesting that LAF cells contain ASCs. Although freshly isolated PLA and LAF cells have distinct cell surface marker profiles, adherent PLA and LAF cells have quite similar characteristics with regard to growth kinetics, morphology, capacity for differentiation, and surface marker profiles. After plating, both PLA and LAF cells showed significant increased expression of CD29, CD44, CD49d, CD73, CD90, CD105, and CD151 and decreased expression of CD31 and CD45. Multicolor FACS analysis revealed that SVF are composed of heterogeneous cell populations including blood-derived cells (CD45(+)), ASCs (CD31(-)CD34(+)CD45(-)CD90(+)CD105(-)CD146(-)), endothelial (progenitor) cells (CD31(+)CD34(+)CD45(-) CD90(+)CD105(low)CD146(+)), pericytes (CD31(-)CD34(-)CD45(-)CD90(+)CD105(-)CD146(+)), and other cells. After plating, ASCs showed a dramatic increase in CD105 expression. Although some adherent ASCs lost CD34 expression with increasing culture time, our culture method maintained CD34 expression in ASCs for at least 10-20 weeks. These results suggest that liposuction-derived cells may be useful and valuable for cell-based therapies.
  • Masakazu Kurita, Emiko Aiba, Daisuke Matsumoto, Katsujiro Sato, Takashi Nagase, Kotaro Yoshimura
    PLASTIC AND RECONSTRUCTIVE SURGERY 117 (6) 107E - 111E 0032-1052 2006/05 [Refereed][Not invited]
  • K Yoshimura, J DeMeester, JS Hasan, MG Urbanchek, WM Kuzon
    PLASTIC AND RECONSTRUCTIVE SURGERY 117 (5) 1455 - 1461 0032-1052 2006/04 [Refereed][Not invited]
     
    Background: The mechanisms responsible for the incomplete recovery of muscle function after microneurovascular transfer have not been fully determined. Because fiber degeneration and regeneration can impact muscle mechanical function, the authors tested the null hypothesis that ischeinia-induced fiber degeneration is not responsible for the force deficits observed after neurovascular muscle transfer. Methods: Rats were assigned to one of three groups: orthotopic. nonvascularized grafting of the extensor digitorum longus muscle (STD group); orthotopic, nenrovascular transfer of the extensor digitorum longus muscle With no intra-operative ischemia (NV-0 group); or orthotopic, neurovascular transfer of the extensor digitorum longus muscle with 3 hours of intraoperative ischemia (NV-3 group. At 1 and 2 weeks, extensor digitorum longus muscle cross-sections were labeled For developmental in),myosin heavy chain isoforms, markers of fiber regeneration. Results: In extensor (digitorum longus muscles from animals in the STD group, many small cells strongly), labeled for developmental myosin heavy chain were observed and identified as inyoblasts, indicating recent muscle fiber necrosis With Subsequent regeneration. Extensor digitorum longus muscles from rats in the NV-0 and NV-3 groups contained no cells labeled for developmental myosin heavy chain. Conclusions: After neurovascular muscle transfer (with ischemia times up to 3 hours), ischemia-induced muscle fiber degeneration and regeneration does not Occur. muscle fiber degeneration is not responsible for the force deficits observed after microneurovascular skeletal muscle transfer.
  • K Yoshimura, K Sato, E Aiba-Kojma, D Matsumoto, C Machino, T Nagase, K Gonda, Koshima, I
    DERMATOLOGIC SURGERY 32 (3) 365 - 371 1076-0512 2006/03 [Refereed][Not invited]
     
    BACKGROUND AND OBJECTIVE Melasma and acquired dermal melanocytosis (ADM; acquired bilateral nevus of Ota-like macules) are both seen most commonly symmetrically on the face of women with darker skin and are also known as difficult conditions to treat. METHODS Our topical bleaching protocol with 0.1 to 0.4% tretinoin gel and 5% hydroquinone was performed repeatedly (1-3 times) for melasma (n=163), and a combination treatment with topical bleaching and Q-switched ruby (QSR) laser was performed repeatedly (1-3 times) for ADM (n = 62). RESULTS There is a significant correlation between clinical results (clearance of pigmentation) and the number of sessions in both melasma (p=.019) and ADM (p <.0001). CONCLUSION The repeated treatment protocol for melasma and ADM showed successful clinical results compared with conventional ones, and they may be applied to other pigment conditions. It may be better that epidermal and dermal pigmentations are treated separately, especially in dark-skinned people who are more likely to suffer postinflammatory hyperpigmentation after inflammation-inducing therapies.
  • M Okazaki, Y Suzuki, K Yoshimura, K Harii
    SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY 39 (6) 339 - 343 0284-4311 2005/12 [Refereed][Not invited]
     
    We have constructed a pigmented skin equivalent and used it to study the hyperpigmentation seen in cafe-au-lait macules to elucidate whether the pigmented skin equivalent could be used as a model of congenital hyperpigmentary disorders. When we used fibroblasts derived from cafe-au-lait macules of neurofibromatosis type 1, the amount of pigment was significantly greater than in models using cells derived from normal skin. Quantities of pigment were not seen when keratinocytes derived from solitary cafe-au-lait macules were used, a possible reason being that keratinocytes on the skin equivalent are in a proliferating condition and are not well-differentiated enough to act on other cells. Our results suggested that our pigmented skin equivalent is useful for the study of congenital hyperpigmentary disorders, although insufficient differentiation of keratinocytes might be a disadvantage.
  • M Okazaki, K Yoshimura, G Uchida, Y Suzuki, Y Kitano, K Harii
    SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY 39 (4) 213 - 217 0284-4311 2005/09 [Refereed][Not invited]
     
    To clarify the mechanism of accentuated melanisation in non-syndromic solitary cafe-au-lait macules we used an enzyme-linked immunosorbent assay (ELISA) to measure the concentration of melanogenic cytokines secreted by cultured keratinocytes and fibroblasts derived from the skins of the macules and compared them with those derived from normal people. Endothelin-1 (ET-1) was significantly increased in cultured keratinocytes in the macules compared with the normals. In contrast, the secretion of other cytokines secreted by keratinocytes or fibroblasts did not differ between the groups. It may be that the increased secretion of ET-1 by epidermal keratinocytes has a role in the accentuated epidermal melanisation seen in non-syndromic macules.
  • Nagase T, Nagase M, Yoshimura K, Fujita T, Koshima I
    Genes to cells : devoted to molecular & cellular mechanisms 10 (6) 595 - 604 1356-9597 2005/06 [Refereed][Not invited]
  • Y Mochizuki, K Ojima, A Uezumi, S Masuda, K Yoshimura, S Takeda
    AMERICAN JOURNAL OF PATHOLOGY 166 (6) 1721 - 1732 0002-9440 2005/06 [Refereed][Not invited]
     
    In denervated skeletal muscle, mononuclear interstitial cells accumulate in the perisynaptic regions before fibrotic change occurs. These cells are currently considered to be fibroblasts that originate from muscle tissue. However, when we denervated hind limbs of GFP-bone marrow chimeric mice by excising the sciatic nerve unilaterally, many bone marrow-derived cells (BM-DCs) infiltrated the interstitial spaces and accumulated in the perisynaptic regions, peaking 14 days after denervation. They accounted for nearly one-half of the increase in mononuclear interstitial cells. Although BM-DCs did not incorporate into satellite cells, immunohistochemical and FACS analyses revealed that BM-DCs were both CD45 and CD11b positive, indicating that they were of macrophage/ monocyte lineage. BrdU staining showed inactive proliferation of BM-DCs. Reverse transcriptase-polymerase chain reaction of mononuclear cells isolated by FACS revealed that BM-DCs did not express type I collagen or tenascin-C; however, they did express transforming growth factor-beta 1, suggesting that they regulate the fibrotic process. In contrast, muscle tissue-derived interstitial cells expressed type I collagen and tenascin-C, suggesting that these populations were the final effectors of fibrosis. These findings identify elementary targets that may regulate the migration, homing, differentiation, and function of BM-DCs, leading to amelioration of the excessive fibrosis of denervated skeletal muscle.
  • K Yoshimura, S Wakita, K Sato, N Kaji, E Aiba, D Matsumoto, H Yamaoka, T Nagase
    SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY 39 (2) 77 - 84 0284-4311 2005/04 [Refereed][Not invited]
     
    The problem of postoperative reduction of projecting reconstructed nipples remains to be resolved. To this end we did a clinical study of reconstructing the nipple at the same time as the breast. A tissue-expander was placed under the skin of the breast at the first operation, and then the breast and nipple were reconstructed at the second. A nipple was reconstructed using a dermal-fat flap harvested from the myocutaneous flaps used for reconstruction of the breast. A small hole was made in the corresponding site of the skin of the breast, and the reconstructed nipple was projected through the hole. This method was used in 8 cases. This method is useful in reconstructing a breast without a pad of skin and a projected nipple simultaneously. Its disadvantages are the relatively weak blood supply of the flaps, and difficulty in calculating the position of the nipple. The procedure may be beneficial for selected cases.
  • T Nagase, K Yoshimura, E Aiba, D Matsumoto, K Sato, C Machino
    PLASTIC AND RECONSTRUCTIVE SURGERY 115 (2) 633 - 640 0032-1052 2005/02 [Refereed][Not invited]
     
    A prominent mandibular angle is a relatively common aesthetic problem among Asians, and the reduction angle-splitting ostectomy is now becoming a very popular procedure in Asian countries. Although this operation is usually performed on young patients, the same aesthetic demands are also seen in the elderly. In this report, the authors describe their experience with angle-splitting ostectomies followed by face lifts in three patients older than 50 years. The angle-splitting procedure was the same as that performed in young patients, and clinical results were assessed with photographs and three-dimensional computed tomographic scans. The facial contours after angle-splitting ostectomy were satisfactory, but the patients showed postoperative redundancy of the skin, especially along the jaw line, because of the loss of bony protrusion laterally. Therefore, the patients underwent subsequent superficial musculoaponeurotic system cheek lifts. The final aesthetic results were satisfactory in all cases. When surgeons want to perform the angle-splitting ostectomy safely and effectively on the elderly, they should be aware of the risks and indications specific for elderly patients, and a multidisciplinary support system should be available. Subsequent face lifts can improve skin redundancy and lead to better cosmetic results.
  • T Nagase, M Nagase, N Osumi, S Fukuda, S Nakamura, K Ohsaki, K Harii, H Asato, K Yoshimura
    JOURNAL OF CRANIOFACIAL SURGERY 16 (1) 80 - 88 1049-2275 2005/01 [Refereed][Not invited]
     
    The pathogenesis of holoprosencephaly is multifactorial, and blockage of Sonic hedgehog signaling is one of the most important causative factors in animal models and human cases. In this study, the authors analyzed facial anomalies of mouse embryos, which were cultured in vitro and exposed to cyclopamine, an alkaloid blocker of Sonic hedgehog signaling. When cultured with cyclopamine for embryonic day 8.5 to 10.5, the whole body size was smaller than normal, and the distance and angle between the nasal placodes were remarkably reduced. Extension of the cranial surface vessels also was noted. No cyclopia was observed. Migration of the cranial neural crest cells seemed to be intact. Expressions of Patched-1 and Gli-1, downstream genes of Sonic hedgehog signaling, also were down-regulated in in situ hybridization and real-time reverse transcriptase-polymerase chain reaction analyses. The authors consider that these facial anomalies represent milder phenotypes of holoprosencephaly.
  • T Hirohi, K Yoshimura
    AESTHETIC PLASTIC SURGERY 28 (1) 58 - 58 0364-216X 2004/01 [Refereed][Not invited]
  • A Momosawa, K Yoshimura, G Uchida, K Sato, E Aiba, D Matsumoto, H Yamaoka, S Mihara, K Tsukamoto, K Harii, T Aoyama, T Iga
    DERMATOLOGIC SURGERY 29 (10) 1001 - 1007 1076-0512 2003/10 [Refereed][Not invited]
     
    BACKGROUND AND OBJECTIVE. Acquired dermal melanocytosis (ADM; acquired bilateral nevus of Ota-like macules) is known for its recalcitrance compared with Nevus of Ota, and we assume that one of the reasons is a higher rate and degree of postinflammatory hyperpigmentation (PIH) seen after laser treatments. METHODS. Topical bleaching treatment with 0.1% tretinoin aqueous gel and 5% hydroquinone ointment containing 7% lactic acid was initially performed ( 4 to 6 weeks) to discharge epidermal melanin. Subsequently, Q-switched ruby (QSR) laser was irradiated to eliminate dermal pigmentation. Both steps were repeated two to three times until patient satisfaction was obtained ( usually at a 2-month interval for laser sessions). This treatment was performed in 19 patients with ADM. Skin biopsy was performed in six cases at baseline, after the bleaching pretreatment, and at the end of treatment. RESULTS. All patients showed good to excellent clearing after two to three sessions of QSR laser treatments. The total treatment period ranged from 3 to 13 ( mean of 8.3) months. PIH was observed in 10.5% of the cases. Histologically, epidermal hyperpigmentation was observed in all specimens and was dramatically improved by the topical bleaching pretreatment. CONCLUSION. QSR laser combined with the topical bleaching pretreatment appeared to treat ADM consistently with a low occurrence rate of PIH and lessen the number of laser sessions and total treatment period and may also be applied to any other lesions with both epidermal and dermal pigmentation.
  • T Hirohi, K Yoshimura
    AESTHETIC PLASTIC SURGERY 27 (5) 418 - 422 0364-216X 2003/09 [Refereed][Not invited]
     
    Among concerned nasal appearances, a deformity with supero-lateral displacement of the nostril rim, called retracted nostril rim or elevated nostril rim is commonly seen and is considered one of the most difficult types of cases to treat aesthetically. A new surgical method for treating retracted nostril rim was performed in 10 patients, using the combination of auricular composite graft, internal fixation with a retainer, and external continuing suspension with anchoring sutures. The procedure was successful in maintaining the grafted cartilage in the ideal position and in avoiding recurrence of retraction or elevation of the constructed alar rim. The presented method merits consideration as a standard operative approach for correction of retracted nostril rim.
  • M Okazaki, K Yoshimura, Y Suzuki, K Harii
    PLASTIC AND RECONSTRUCTIVE SURGERY 112 (3) 784 - 792 0032-1052 2003/09 [Refereed][Not invited]
     
    The stratified squamous epithelia differ regionally in their patterns of morphogenesis and differentiation. Although some reports suggested that the adult epithelial phenotype is an intrinsic property of the epithelium, there is increasing evidence that subepithelial connective tissue can modify the phenotypic expression of the epithelium. The aim of this study was to elucidate whether the differentiation of cutaneous and oral epithelia is influenced by underlying mesenchymal tissues. Three normal skin samples and three normal buccal mucosa samples were used for the experiments. Skin equivalents were constructed in four ways, depending on the combinations of keratinocytes (cutaneous or mucosal keratinocytes) and fibroblasts (dermal or mucosal fibroblasts), and the effects of subepithelial fibroblasts on the differentiation of oral and cutaneous keratinocytes were studied with histological examinations and immunohistochemical analyses with anti-cytokeratin (keratins 10 and 13) antibodies. For each experiment, three paired skin equivalents were constructed by using single parent keratinocyte and fibroblast sources for each group; consequently, nine (3 X 3) organotypic cultures per group were constructed and studied. The oral and cutaneous epithelial cells maintained their intrinsic keratin expression. The keratin expression patterns in oral and cutaneous epithelia of skin equivalents were generally similar to their original patterns but were partly modified exogenously by the topologically different fibroblasts. The mucosal keratinocytes were more differentiated and expressed keratin 10 when cocultured with dermal fibroblasts, and the expression patterns of keratin 13 in cutaneous keratinocytes cocultured with mucosal fibroblasts were different from those in keratinocytes cocultured with cutaneous fibroblasts. The results suggested that the epithelial phenotype and keratin expression could be extrinsically modified by mesenchymal fibroblasts. In epithelial differentiation, however, the intrinsic control by epithelial cells. may still be stronger than extrinsic regulation by mesenchymal fibroblasts.
  • Yoshimura K, Momosawa A, Aiba E, Sato K, Matsumoto D, Mitoma Y, Harii K, Aoyama T, Iga T
    Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] 29 155 - 60; discussion 160 1076-0512 2003/02 [Refereed][Not invited]
  • Induction of hard keratin in non-nail-matrical keratinocytes by nail-matrical fibroblasts through epithelial-mesenchymal interaction.
    Okazaki M, Yoshimura K, Fujiwara H, Suzuki Y, Harii K
    Plast Reconstr Surg 111 286 - 290 2003 [Not refereed][Not invited]
  • G Uchida, K Yoshimura, Y Kitano, M Okazaki, K Harii
    EXPERIMENTAL DERMATOLOGY 12 35 - 42 0906-6705 2003 [Refereed][Not invited]
     
    Keloids are skin abnormalities that are characterized by excessive deposition of collagen bundles in the dermis. Patients with keloids complain not only about their cosmetic appearance, but also about continuous itching and/or tenderness associated with chronic inflammation. Degradation of extracellular matrix (ECM) may be upregulated, associated with the expansion of keloids into circumferential skin, and high metabolic activity of keloid tissues may be due to increased matrix metalloproteinase (NIMP) activity. Based on these hypotheses, we examined differences in expression of MMP-1, MMP-8, and MMP-13 between keloid-derived and normal dermal fibroblasts. Since retinoids are potent inhibitors of MMPs in the treatment of photoaged skin and cancers, we also examined whether or not tretinoin affects MMP expression of keloid-derived fibroblasts. The results of real-time polymerase chain reaction and ELISA demonstrated significant upregulation of MMP-13 and significant downregulation of MMP-1 and MMP-8 in keloid-derived fibroblasts, at both mRNA and protein levels. MMP-1 mRNA expression in the control group was significantly upregulated after the addition of tretinoin, whereas no significant change was observed in the keloid group. MMP-8 mRNA expression in the control group was significantly upregulated by tretinoin, with the peak at 12 h. while no significant change was observed in the keloid-derived fibroblasts. In contrast, the remarkably elevated MMP-13 mRNA expression in the keloid group was significantly suppressed, with the peak suppression at 12 h after addition of tretinoin, while MMP-13 mRNA expression in the control group was not significantly changed. The decrease in MMP-1 and MMP-8 may contribute to accumulation of type I and type III collagen in keloid tissues, and this mechanism may be modulated by molecular interaction with MMP-13. Tretinoin appeared to reverse the abnormal expression profile of MMPs in keloid-derived fibroblasts, such as markedly elevated expression of MMP-13, partly through inactivation of AP-1 pathway. The present results suggest that tretinoin may be clinically useful to improve the chronic inflammation seen in keloids and prevent expansion of keloid tissues into circumferential normal skin.
  • K Yoshimura, G Uchida, M Okazaki, Y Kitano, K Harii
    EXPERIMENTAL DERMATOLOGY 12 28 - 34 0906-6705 2003 [Refereed][Not invited]
     
    It was recently revealed that epidermal growth following topical treatment with all-trans retinoic acid (atRA) was at least partly induced by heparin-binding epidermal growth factor-like growth factor (HB-EGF) released from suprabasal keratinocytes. Since proliferation of keratinocytes appears to be one of the critical roles of atRA in depigmentation treatment and promotion of wound healing, HB-EGF is considered suitable for assessing the therapeutic value of topical retinoids. In this study, HB-EGF mRNA expression in normal human keratinocytes after atRA treatment was examined, and the effects of a variety of natural and synthetic retinoids were compared. The results of reverse transcription polymerase chain reaction (RT-PCR) suggested that induction of differentiation increased HB-EGF mRNA expression in cultured keratinocytes. Real-time PCR analyses revealed that HB-EGF mRNA expression was elevated dose-dependently with atRA, peaking at 12 h. This elevation was more prominent in confluent keratinocytes than in subconfluent cells, suggesting that differentiated keratinocytes are more subject to stimulation of HB-EGF expression by atRA than proliferating keratinocytes. HB-EGF mRNA was upregulated in differentiation-induced keratinocytes by all retinoids used in this study at 1 mumol/l, and marked upregulation was seen when treated with three isotypes of retinoic acid (atRA, and 9-cis and 13-cis retinoic acid). RARalpha-selective agonists (Am80, Am580, ER-38925, and TAC-101) and a panagonist of RARs (Re80) caused relatively low elevation of HB-EGF transcripts, as did all-trans retinol (Rol) and all-trans retinal (Ral). Although another panagonist (Ch55) showed the highest elevation of HB-EGF mRNA, it was relatively cytotoxic at the concentration employed. Ral and Rol were found to upregulate HB-EGF when used at 100 mumol/l to 1 mmol/l, to a similar extent of atRA at 1-10 mumol/l. The capacity of retinoids to upregulate HB-EGF may be an important index for investigation and development of an ideal synthetic retinoid, which has maximum benefits and minimum side-effects.
  • M Okazaki, K Yoshimura, H Fujiwara, Y Suzuki, K Harii
    PLASTIC AND RECONSTRUCTIVE SURGERY 111 (1) 286 - 290 0032-1052 2003/01 [Refereed][Not invited]
     
    Epithelium in the nail matrix is different from that at other body sites, in terms of clinical and histological appearance. Hard keratins are exclusively expressed in the nail matrix and bed and the hair apparatus, and hard keratin is considered a differentiation marker of these sites. Whether the expression of hard keratin in non-nail-matrical keratinocytes could be induced by nail-matrical fibroblasts v,,as examined. Skin equivalents were constructed in three ways; ventral keratinocytes (from the ventral side of the digit) were cocultured with ventral fibroblasts (group A), ventral keratinocytes were cocultured with nail-matrical fibroblasts (group B), and nail-matrical keratinocytes were cocultured with ventral Fibroblasts (group C). Immunohistochemical examinations with anti-hard keratin antibody (HKN-7) revealed hard keratin expression in groups B and C. HKN-7-positive cells were distributed continuously in the en tire epithelial strata or in the suprabasal layer in group B, whereas HKN-7-positive cells were distributed spottily in group C This study indicates extrinsic hard keratin induction in non-nail-matrical keratinocytes by nail-matrical fibroblasts and suggests that non-nail-matrical epidermal grafts may be effective in the treatment of deepithelized nail injuries. In addition, it is possible that lost nails could be reconstructed with grafts of "tissue-engineered' nail equivalent.
  • Induction of hard keratin in non-nail-matrical keratinocytes by nail-matrical fibroblasts through epithelial-mesenchymal interaction.
    Okazaki M, Yoshimura K, Fujiwara H, Suzuki Y, Harii K
    Plast Reconstr Surg 111 286 - 290 2003 [Not refereed][Not invited]
  • Yoshimura K, Momosawa A, Watanabe A, Sato K, Matsumoto D, Aiba E, Harii K, Yamamoto T, Aoyama T, Iga T
    Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] 28 1153 - 7; discussion 1158 1076-0512 2002/12 [Refereed][Not invited]
  • Mutsumi Okazaki, Kotaro Yoshimura, Gentaro Uchida, Kiyonori Harii
    Journal of Dermatological Science 30 (2) 108 - 115 0923-1811 2002/11 [Not refereed][Not invited]
     
    Oral mucosa heals faster with less scar formation than skin and a hypertrophic scar is very rare in the oral cavity, but its mechanism has not been elucidated enough. To elucidate whether or not there are differences in growth factor expression between fibroblasts derived from buccal mucosal and normal skin, we investigated the expression of hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and stem cell factor (SCF) by cultured fibroblasts. The semiquantitative RT-PCR revealed that the expression of HGF and KGF transcripts by buccal mucosal fibroblasts was significantly elevated compared with that by dermal fibroblasts. In parallel, ELISA revealed the significant increase of HGF production by buccal mucosal fibroblasts. The level of production of SCF protein did not differ significantly. Our study suggests that increased expression of HGF and KGF by buccal mucosal fibroblasts may partly be responsible for the faster wound healing with less scar formation in the oral cavity compared with normal skin. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
  • M Okazaki, K Yoshimura, G Uchida, K Harii
    JOURNAL OF DERMATOLOGICAL SCIENCE 30 (2) 108 - 115 0923-1811 2002/11 [Refereed][Not invited]
     
    Oral mucosa heals faster with less sear formation than skin and a hypertrophic scar is very rare in the oral cavity, but its mechanism has not been elucidated enough. To elucidate whether or not there are differences in growth factor expression between fibroblasts derived from buccal mucosal and normal skin, we investigated the expression of hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and stem cell factor (SCF) by cultured fibroblasts. The semiquantitative RT-PCR revealed that the expression of HGF and KGF transcripts by buccal mucosal fibroblasts was significantly elevated compared with that by dermal fibroblasts. In parallel, ELISA revealed the significant increase of HGF production by buccal mucosal fibroblasts. The level of production of SCF protein did not differ significantly. Our study suggests that increased expression of HGF and KGF by buccal mucosal fibroblasts may partly be responsible for the faster wound healing with less scar formation in the oral cavity compared with normal skin. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
  • S Park, T Iida, K Yoshimura, Y Kawasaki
    ANNALS OF PLASTIC SURGERY 48 (6) 582 - 585 0148-7043 2002/06 [Not refereed][Not invited]
     
    Many methods have been reported for the treatment of ganglions. The authors present their modified technique for ganglion sclerotherapy. Their modification enables them to perform sclerotherapy safely and consistently, and they have treated 10 patients in this manner. The method is described and the cases are illustrated.
  • S Park, T Iida, K Yoshimura, Y Kawasaki
    ANNALS OF PLASTIC SURGERY 48 (6) 582 - 585 0148-7043 2002/06 [Not refereed][Not invited]
     
    Many methods have been reported for the treatment of ganglions. The authors present their modified technique for ganglion sclerotherapy. Their modification enables them to perform sclerotherapy safely and consistently, and they have treated 10 patients in this manner. The method is described and the cases are illustrated.
  • K Yoshimura, H Asato, SS Jejurikar, PS Cederna, MG Urbanchek, WM Kuzon
    PLASTIC AND RECONSTRUCTIVE SURGERY 109 (1) 212 - 219 0032-1052 2002/01 [Not refereed][Not invited]
     
    Sensory or motor "baby-sitting" has been proposed its a clinical strategy to preserve muscle integrity if motion-specific axons must regenerate over a long distance to reach denervated target muscles. Denervated muscles are innervated temporarily by using axons from nearby sensory or motor nerves. After motion specific motor axons have reached the target, the baby-sitter nerve is severed and motion-specific axons are directed to [lie target. Although this strategy minimizes denervation time, the requisite second episode of denervation and reinnervation might be deleterious to muscle contractile function. This study was designed to test the hypothesis that two sequential episodes of skeletal muscle denervation and reinnervation result in greater force and power deficits than a single peripheral nerve injury and repair. Adult Lewis rats underwent either transection and epineurial repair or sham exposure of the left peroneal nerve. After a 4-month recovery period, the contractile properties of the extensor digitorum longus muscle of the sham exposure group (control, n = 9) and one of the nerve division and repair groups (repair group 1, n = 9) were evaluated with measurements of the maximum tetanic isometric force, peak power, and maximal sustained power. A third group of rats Underwent a second cycle of nerve division and repair (repair group 2, n = 9) at this same time point. Four months postoperatively, contractile properties of the extensor digitorum longus muscles were evaluated. Maximum tetanic isometric force and peak power were significantly reduced in repair group 2 rats as compared with repair group I and control rats. Maximal sustained power was not significantly different between the groups. These data Support our working hypothesis that skeletal muscle contractile function is adversely affected by two cycles of denervation and reinnervation as compared with a single episode of nerve division and repair.
  • K Yoshimura, H Asato, SS Jejurikar, PS Cederna, MG Urbanchek, WM Kuzon
    PLASTIC AND RECONSTRUCTIVE SURGERY 109 (1) 212 - 219 0032-1052 2002/01 [Refereed][Not invited]
     
    Sensory or motor "baby-sitting" has been proposed its a clinical strategy to preserve muscle integrity if motion-specific axons must regenerate over a long distance to reach denervated target muscles. Denervated muscles are innervated temporarily by using axons from nearby sensory or motor nerves. After motion specific motor axons have reached the target, the baby-sitter nerve is severed and motion-specific axons are directed to [lie target. Although this strategy minimizes denervation time, the requisite second episode of denervation and reinnervation might be deleterious to muscle contractile function. This study was designed to test the hypothesis that two sequential episodes of skeletal muscle denervation and reinnervation result in greater force and power deficits than a single peripheral nerve injury and repair. Adult Lewis rats underwent either transection and epineurial repair or sham exposure of the left peroneal nerve. After a 4-month recovery period, the contractile properties of the extensor digitorum longus muscle of the sham exposure group (control, n = 9) and one of the nerve division and repair groups (repair group 1, n = 9) were evaluated with measurements of the maximum tetanic isometric force, peak power, and maximal sustained power. A third group of rats Underwent a second cycle of nerve division and repair (repair group 2, n = 9) at this same time point. Four months postoperatively, contractile properties of the extensor digitorum longus muscles were evaluated. Maximum tetanic isometric force and peak power were significantly reduced in repair group 2 rats as compared with repair group I and control rats. Maximal sustained power was not significantly different between the groups. These data Support our working hypothesis that skeletal muscle contractile function is adversely affected by two cycles of denervation and reinnervation as compared with a single episode of nerve division and repair.
  • K Yoshimura, H Asato, SS Jejurikar, PS Cederna, MG Urbanchek, WM Kuzon
    PLASTIC AND RECONSTRUCTIVE SURGERY 109 (1) 212 - 219 0032-1052 2002/01 [Not refereed][Not invited]
     
    Sensory or motor "baby-sitting" has been proposed its a clinical strategy to preserve muscle integrity if motion-specific axons must regenerate over a long distance to reach denervated target muscles. Denervated muscles are innervated temporarily by using axons from nearby sensory or motor nerves. After motion specific motor axons have reached the target, the baby-sitter nerve is severed and motion-specific axons are directed to [lie target. Although this strategy minimizes denervation time, the requisite second episode of denervation and reinnervation might be deleterious to muscle contractile function. This study was designed to test the hypothesis that two sequential episodes of skeletal muscle denervation and reinnervation result in greater force and power deficits than a single peripheral nerve injury and repair. Adult Lewis rats underwent either transection and epineurial repair or sham exposure of the left peroneal nerve. After a 4-month recovery period, the contractile properties of the extensor digitorum longus muscle of the sham exposure group (control, n = 9) and one of the nerve division and repair groups (repair group 1, n = 9) were evaluated with measurements of the maximum tetanic isometric force, peak power, and maximal sustained power. A third group of rats Underwent a second cycle of nerve division and repair (repair group 2, n = 9) at this same time point. Four months postoperatively, contractile properties of the extensor digitorum longus muscles were evaluated. Maximum tetanic isometric force and peak power were significantly reduced in repair group 2 rats as compared with repair group I and control rats. Maximal sustained power was not significantly different between the groups. These data Support our working hypothesis that skeletal muscle contractile function is adversely affected by two cycles of denervation and reinnervation as compared with a single episode of nerve division and repair.
  • Okazaki M, Yoshimura K, Uchida G, Harii K
    J Dermatol Sci 30 (2) 108 - 115 0923-1811 2002 [Not refereed][Not invited]
  • Effects of all-trans retinoic acid on melanogenesis in pigmented skin equivalents and monolayer culture of melanocytes.
    Yoshimura K, Tsukamoto K, Okazaki M, Virador VM, Lei TC, Suzuki Y, Uchida G, Kitano Y, Harii K
    J Dermatol Sci 27 (S1) 68 - 75 2001 [Not refereed][Not invited]
  • Yoshimura K, Harii K, Masuda Y, Takahashi M, Aoyama T, Iga T
    Aesth Plast Surg 25 129 - 33 2001 [Not refereed][Not invited]
  • Wound healing of genetically diabetic mouse after topical treatment with all-trans retinoic acid.
    Kitano Y, Yoshimura K, Uchida G, Sato K, Harii K
    Arch Dermatol Res. 292 515 - 521 2001 [Not refereed][Not invited]
  • Wound healing of genetically diabetic mouse after topical treatment with all-trans retinoic acid.
    Kitano Y, Yoshimura K, Uchida G, Sato K, Harii K
    Arch Dermatol Res. 292 515 - 521 2001 [Not refereed][Not invited]
  • Yoshimura K, Harii K, Masuda Y, Takahashi M, Aoyama T, Iga T
    Aesth Plast Surg 25 129 - 33 2001 [Not refereed][Not invited]
  • Effects of all-trans retinoic acid on melanogenesis in pigmented skin equivalents and monolayer culture of melanocytes.
    Yoshimura K, Tsukamoto K, Okazaki M, Virador VM, Lei TC, Suzuki Y, Uchida G, Kitano Y, Harii K
    J Dermatol Sci 27 (S1) 68 - 75 2001 [Not refereed][Not invited]
  • K Gonda, T Nakaoka, K Yoshimura, Y Otawara-Hamamoto, K Harrii
    JOURNAL OF BONE AND MINERAL RESEARCH 15 (6) 1056 - 1065 0884-0431 2000/06 [Not refereed][Not invited]
     
    In vivo gene transfer is a recently developed device for efficient delivery of a therapeutic recombinant protein. We formulated the hypothesis that a high level of expression of bone morphogenetic protein 2 (BMP-2) could be a future therapeutic modality in terms of inducing substantial bone formation in vive. First, to test this hypothesis, adenoviruses carrying BMP-2 gene were directly injected into the soleus muscle of adult rat. The BMP-2 gene was successfully overexpressed in the target muscle by adenovirus-mediated transfer, whereas bone formation in and around the muscle failed to occur in this case. Second, to recruit putative osteoprogenitor cells, we then induced ischemic degeneration of the target muscle by orthotopically grafting it simultaneously with the gene transfer. The combination of BMP-2 gene transfer and orthotopic muscle grafting resulted in successful ossification of almost the whole grafted muscle, whereas neither muscle grafting alone nor the combination of muscle grafting and adenovirus-mediated transfer of reporter gene LacZ induced any bone formation in the muscle. The ossification process was evident by positive von Kossa staining of the histological sections and roentgenographical radio-opacity of the region. It was also found that the BMP-2 transgene overexpressed in grafted muscles inhibited muscle regeneration, which should otherwise follow the muscle degeneration. We further demonstrated an up-regulation of BMP receptor type IA in grafted muscles, suggesting its involvement in the bone-formation process. In conclusion, overexpression of BMP-2 gene induced massive heterotopic ossification in skeletal muscles under graft-induced ischemic degeneration, which possibly up-regulates osteoprogenitor cells in situ.
  • K Gonda, T Nakaoka, K Yoshimura, Y Otawara-Hamamoto, K Harrii
    JOURNAL OF BONE AND MINERAL RESEARCH 15 (6) 1056 - 1065 0884-0431 2000/06 [Not refereed][Not invited]
     
    In vivo gene transfer is a recently developed device for efficient delivery of a therapeutic recombinant protein. We formulated the hypothesis that a high level of expression of bone morphogenetic protein 2 (BMP-2) could be a future therapeutic modality in terms of inducing substantial bone formation in vive. First, to test this hypothesis, adenoviruses carrying BMP-2 gene were directly injected into the soleus muscle of adult rat. The BMP-2 gene was successfully overexpressed in the target muscle by adenovirus-mediated transfer, whereas bone formation in and around the muscle failed to occur in this case. Second, to recruit putative osteoprogenitor cells, we then induced ischemic degeneration of the target muscle by orthotopically grafting it simultaneously with the gene transfer. The combination of BMP-2 gene transfer and orthotopic muscle grafting resulted in successful ossification of almost the whole grafted muscle, whereas neither muscle grafting alone nor the combination of muscle grafting and adenovirus-mediated transfer of reporter gene LacZ induced any bone formation in the muscle. The ossification process was evident by positive von Kossa staining of the histological sections and roentgenographical radio-opacity of the region. It was also found that the BMP-2 transgene overexpressed in grafted muscles inhibited muscle regeneration, which should otherwise follow the muscle degeneration. We further demonstrated an up-regulation of BMP receptor type IA in grafted muscles, suggesting its involvement in the bone-formation process. In conclusion, overexpression of BMP-2 gene induced massive heterotopic ossification in skeletal muscles under graft-induced ischemic degeneration, which possibly up-regulates osteoprogenitor cells in situ.
  • K Yoshimura, K Ouchi, S Wakita, K Uda, K Harii
    PLASTIC AND RECONSTRUCTIVE SURGERY 105 (3) 836 - 841 0032-1052 2000/03 [Not refereed][Not invited]
     
    An approach for the correction of cryptotia using a superiorly based superficial mastoid fascial flap and a skin paddle is introduced. The buried portion of the auricle was exposed through an incision made along the upper part of the helix, followed by an appropriate correction of the deformed cartilage. Protrusion of the upper portion of the auricle was accomplished using anchoring sutures. A small skin paddle was elevated from the caudal portion of the auricular sulcus with the superiorly based superficial mastoid fascia as the nutrient pedicle and transferred to the temporal skin defect. The procedure was performed in eight auricles in a total of seven patients with cryptotia. A satisfactory contour and protrusion of the auricle were maintained postoperatively, leaving the scar within the auricular sulcus.
  • K Yoshimura, K Ouchi, S Wakita, K Uda, K Harii
    PLASTIC AND RECONSTRUCTIVE SURGERY 105 (3) 836 - 841 0032-1052 2000/03 [Not refereed][Not invited]
     
    An approach for the correction of cryptotia using a superiorly based superficial mastoid fascial flap and a skin paddle is introduced. The buried portion of the auricle was exposed through an incision made along the upper part of the helix, followed by an appropriate correction of the deformed cartilage. Protrusion of the upper portion of the auricle was accomplished using anchoring sutures. A small skin paddle was elevated from the caudal portion of the auricular sulcus with the superiorly based superficial mastoid fascia as the nutrient pedicle and transferred to the temporal skin defect. The procedure was performed in eight auricles in a total of seven patients with cryptotia. A satisfactory contour and protrusion of the auricle were maintained postoperatively, leaving the scar within the auricular sulcus.
  • Kawasaki Y, Yoshimura K, Harii K, Park S
    J Hand Surg (Am) 25A 104 - 111 2000 [Not refereed][Not invited]
  • Experience of a Strong Bleaching Treatment for Skin Hyperpigmentation in Orientals.
    Yoshimura K, Harii K, Aoyama T, Iga T
    Plast Reconstr Surg 105 1097 - 1110 2000 [Not refereed][Not invited]
  • Experience of a Strong Bleaching Treatment for Skin Hyperpigmentation in Orientals.
    Yoshimura K, Harii K, Aoyama T, Iga T
    Plast Reconstr Surg 105 1097 - 1110 2000 [Not refereed][Not invited]
  • Kawasaki Y, Yoshimura K, Harii K, Park S
    J Hand Surg (Am) 25A 104 - 111 2000 [Not refereed][Not invited]
  • K Yoshimura, H Asato, T Nakatsuka, Y Sugawara, S Park
    BRITISH JOURNAL OF PLASTIC SURGERY 52 (7) 530 - 533 0007-1226 1999/10 [Not refereed][Not invited]
     
    Two-stage methods for reconstruction of congenital microtia have been widely utilised. To obtain a desirable auriculocephalic angle and provide a nutrient support to the constructed auricle, elevation of reconstructed ears using a costal cartilage graft, the anteriorly based mastoid fascial flap transfer and a skin graft was performed as the second operation for nine microtia patients. In this procedure, the mastoid fascial flap was used instead of the temporoparietal fascial flap. Following the elevation of the reconstructed ear the anteriorly based mastoid fascial flap was harvested. A carved costal cartilage was grafted at the posterior wall of the concha and covered with the mastoid fascial flap, followed by a full-thickness skin graft from the inguinal region. The skin grafts took well and the appropriate auriculocephalic angle was preserved in all cases. This method was easy to perform and did not leave any scar in the temporal hair-bearing area. (C) 1999 The British association of Plastic Surgeons.
  • K Yoshimura, H Asato, T Nakatsuka, Y Sugawara, S Park
    BRITISH JOURNAL OF PLASTIC SURGERY 52 (7) 530 - 533 0007-1226 1999/10 [Not refereed][Not invited]
     
    Two-stage methods for reconstruction of congenital microtia have been widely utilised. To obtain a desirable auriculocephalic angle and provide a nutrient support to the constructed auricle, elevation of reconstructed ears using a costal cartilage graft, the anteriorly based mastoid fascial flap transfer and a skin graft was performed as the second operation for nine microtia patients. In this procedure, the mastoid fascial flap was used instead of the temporoparietal fascial flap. Following the elevation of the reconstructed ear the anteriorly based mastoid fascial flap was harvested. A carved costal cartilage was grafted at the posterior wall of the concha and covered with the mastoid fascial flap, followed by a full-thickness skin graft from the inguinal region. The skin grafts took well and the appropriate auriculocephalic angle was preserved in all cases. This method was easy to perform and did not leave any scar in the temporal hair-bearing area. (C) 1999 The British association of Plastic Surgeons.
  • S Ichioka, T Nakatsuka, K Yoshimura, N Kaji, K Harii
    ANNALS OF PLASTIC SURGERY 43 (1) 83 - 86 0148-7043 1999/07 [Not refereed][Not invited]
     
    Reconstruction of oral scar contracture is often a challenging problem due to the complex structures and functions of the oral cavity. This report describes the treatment of a patient who sustained extensive oral scar contracture following caustic liquid soda ingestion, Surgical release of the scar contracture formed an S-shaped, thin, long defect that was difficult to cover with a conventional flap or skin graft. A jejunal segment was transferred microsurgically as a patch to reconstruct the defect, It sustained a sufficient oral space to provide full opening of the mouth and goad movement of the tongue. A free jejunal flap, used occasionally for reconstruction following oral cancer resection, has significant advantages for restoration of function after release of an oral scar contracture.
  • S Ichioka, T Nakatsuka, K Yoshimura, N Kaji, K Harii
    ANNALS OF PLASTIC SURGERY 43 (1) 83 - 86 0148-7043 1999/07 [Not refereed][Not invited]
     
    Reconstruction of oral scar contracture is often a challenging problem due to the complex structures and functions of the oral cavity. This report describes the treatment of a patient who sustained extensive oral scar contracture following caustic liquid soda ingestion, Surgical release of the scar contracture formed an S-shaped, thin, long defect that was difficult to cover with a conventional flap or skin graft. A jejunal segment was transferred microsurgically as a patch to reconstruct the defect, It sustained a sufficient oral space to provide full opening of the mouth and goad movement of the tongue. A free jejunal flap, used occasionally for reconstruction following oral cancer resection, has significant advantages for restoration of function after release of an oral scar contracture.
  • S Ichioka, T Nakatsuka, K Yoshimura, N Kaji, K Harii
    ANNALS OF PLASTIC SURGERY 43 (1) 83 - 86 0148-7043 1999/07 [Not refereed][Not invited]
     
    Reconstruction of oral scar contracture is often a challenging problem due to the complex structures and functions of the oral cavity. This report describes the treatment of a patient who sustained extensive oral scar contracture following caustic liquid soda ingestion, Surgical release of the scar contracture formed an S-shaped, thin, long defect that was difficult to cover with a conventional flap or skin graft. A jejunal segment was transferred microsurgically as a patch to reconstruct the defect, It sustained a sufficient oral space to provide full opening of the mouth and goad movement of the tongue. A free jejunal flap, used occasionally for reconstruction following oral cancer resection, has significant advantages for restoration of function after release of an oral scar contracture.
  • T Nakatsuka, K Harii, A Takushima, K Yoshimura, S Ichioka, M Sugasawa, K Ichimura, Y Seto, H Nagawa
    PLASTIC AND RECONSTRUCTIVE SURGERY 103 (2) 458 - 464 0032-1052 1999/02 [Not refereed][Not invited]
     
    A new method that uses a prefabricated free jejunal transfer has been applied to three cancer patients with pharyngoesophageal defects with a high pharyngeal deficit extending up to the nasopharynx. In this method, the jejunum harvested in the usual manner is divided into two segments with a single vascular pedicle. Its distal segment is used to reconstruct the cervical esophagus, and its proximal segment is turned over to create a mucosal patch to cover the high pharyngeal defects. The two segments are then co-apted in a side-by-side anastomosis. The esophagus can be reconstructed in a naturally straight shape without a curved portion or blind loop formation, thus leading to good swallowing function. In our series, all grafts survived well and there was no complication directly related to jejunal transfer. All patients could tolerate a soft diet without difficulty. This method is easy to perform and applicable to any shape or size of very high pharyngeal defects that cannot be reconstructed properly by other methods. Although patients with an advanced hypopharyngeal cancer usually have a poor prognosis, this technique allows a better quality of life for a probable short life span.
  • K Yoshimura, H Asato, PS Cederna, MG Urbanchek, WM Kuzon
    JOURNAL OF SURGICAL RESEARCH 81 (2) 201 - 208 0022-4804 1999/02 [Not refereed][Not invited]
     
    Failure to fully restore contractile function after denervation and reinnervation of skeletal muscle engenders significant disability in patients suffering peripheral nerve injuries. This work tested the hypothesis that skeletal muscle denervation and reinnervation result in a deficit in normalized power (W/kg), which exceeds the deficit in specific force (N/cm(2)), and that the mechanisms responsible for these deficits are independent. Adult Lewis rats underwent either transection and epineurial repair of the left peroneal nerve (denervation-reinnervation, n = 13) or SHAM: exposure of the peroneal nerve (SHAM, n = 13). After a 4-month recovery period, isometric force, peak power, and maximum sustained power output were measured in the left extensor digitorum longus (EDL) muscle from each animal. Isometric force measurements revealed a specific force deficit of 14.3% in the reinnervated muscles. Power measurements during isovelocity shortening contractions demonstrated a normalized peak power deficit of 25.8% in the reinnervated muscles, which is accounted for by decreases in both optimal velocity (10.5%) and average force curing shortening (13.7%). Maximum sustained power was similar in both groups. These data support our working hypothesis that both whole muscle force production and power output can be impaired in reinnervated muscle and that the relative deficits in power output exceed the deficits in force production. The mechanisms responsible for the deficits in force production appear to be independent of those that result in changes in peak power output. The measurement of muscle power output may represent a clinically relevant variable for studies of the recovery of mechanical function after motor nerve injury and repair. (C) 1999 Academic Press.
  • K Yoshimura, H Asato, PS Cederna, MG Urbanchek, WM Kuzon
    JOURNAL OF SURGICAL RESEARCH 81 (2) 201 - 208 0022-4804 1999/02 [Not refereed][Not invited]
     
    Failure to fully restore contractile function after denervation and reinnervation of skeletal muscle engenders significant disability in patients suffering peripheral nerve injuries. This work tested the hypothesis that skeletal muscle denervation and reinnervation result in a deficit in normalized power (W/kg), which exceeds the deficit in specific force (N/cm(2)), and that the mechanisms responsible for these deficits are independent. Adult Lewis rats underwent either transection and epineurial repair of the left peroneal nerve (denervation-reinnervation, n = 13) or SHAM: exposure of the peroneal nerve (SHAM, n = 13). After a 4-month recovery period, isometric force, peak power, and maximum sustained power output were measured in the left extensor digitorum longus (EDL) muscle from each animal. Isometric force measurements revealed a specific force deficit of 14.3% in the reinnervated muscles. Power measurements during isovelocity shortening contractions demonstrated a normalized peak power deficit of 25.8% in the reinnervated muscles, which is accounted for by decreases in both optimal velocity (10.5%) and average force curing shortening (13.7%). Maximum sustained power was similar in both groups. These data support our working hypothesis that both whole muscle force production and power output can be impaired in reinnervated muscle and that the relative deficits in power output exceed the deficits in force production. The mechanisms responsible for the deficits in force production appear to be independent of those that result in changes in peak power output. The measurement of muscle power output may represent a clinically relevant variable for studies of the recovery of mechanical function after motor nerve injury and repair. (C) 1999 Academic Press.
  • T Nakatsuka, K Harii, A Takushima, K Yoshimura, S Ichioka, M Sugasawa, K Ichimura, Y Seto, H Nagawa
    PLASTIC AND RECONSTRUCTIVE SURGERY 103 (2) 458 - 464 0032-1052 1999/02 [Not refereed][Not invited]
     
    A new method that uses a prefabricated free jejunal transfer has been applied to three cancer patients with pharyngoesophageal defects with a high pharyngeal deficit extending up to the nasopharynx. In this method, the jejunum harvested in the usual manner is divided into two segments with a single vascular pedicle. Its distal segment is used to reconstruct the cervical esophagus, and its proximal segment is turned over to create a mucosal patch to cover the high pharyngeal defects. The two segments are then co-apted in a side-by-side anastomosis. The esophagus can be reconstructed in a naturally straight shape without a curved portion or blind loop formation, thus leading to good swallowing function. In our series, all grafts survived well and there was no complication directly related to jejunal transfer. All patients could tolerate a soft diet without difficulty. This method is easy to perform and applicable to any shape or size of very high pharyngeal defects that cannot be reconstructed properly by other methods. Although patients with an advanced hypopharyngeal cancer usually have a poor prognosis, this technique allows a better quality of life for a probable short life span.
  • Kazuki Ueda, Kiyonori Harii, Hirotaka Asato, Kotarou Yoshimura, Atsushi Yamada
    Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 33 (1) 47 - 57 0284-4311 1999 [Not refereed][Not invited]
     
    To acquire symmetry of the cheek when smiling, we carried out 39 free vascularised grafts of the muscle, the motor nerve of which was sutured to a stump of the ipsilateral facial nerve, for 39 patients with facial paralysis. We used two methods: an as healthy and fresh as possible facial nerve stump (method 1A, n = 17) or an incompletely affected stump (method 1B, n = 22). The results are classified into grade 1 to 5 indicating increasing efficiency of muscle function. All patients who had method 1A and 14 patients who had method 1B were evaluated grade 4 or better. Both an incompletely affected facial nerve stump and the proximal stump of a facial nerve that had previously been resected have sufficient function to provide contraction in the grafted muscle.
  • A regenerative change during muscle adaptation to denervation in rats. J Surg Res 81: 139-146, 1999. 10)* Yoshimura K, Harii K, Aoyama T, Shibuya F, Iga T. A new bleaching protocol for hyperpigmented skin lesions with a high concentration of all-trans ・・・
    Yoshimura K, Harii K
    Aesth Plast Surg 23 285 - 291 1999 [Not refereed][Not invited]
     
    A regenerative change during muscle adaptation to denervation in rats. J Surg Res 81: 139-146, 1999. 10)* Yoshimura K, Harii K, Aoyama T, Shibuya F, Iga T. A new bleaching protocol for hyperpigmented skin lesions with a high concentration of all-trans retinoic acid aqueous gel.
  • A regenerative change during muscle adaptation to denervation in rats. J Surg Res 81: 139-146, 1999. 10)* Yoshimura K, Harii K, Aoyama T, Shibuya F, Iga T. A new bleaching protocol for hyperpigmented skin lesions with a high concentration of all-trans ・・・
    Yoshimura K, Harii K
    Aesth Plast Surg 23 285 - 291 1999 [Not refereed][Not invited]
     
    A regenerative change during muscle adaptation to denervation in rats. J Surg Res 81: 139-146, 1999.
    10)* Yoshimura K, Harii K, Aoyama T, Shibuya F, Iga T. A new bleaching protocol for hyperpigmented skin lesions with a high concentration of all-trans retinoic acid aqueous gel.
  • Kazuki Ueda, Kiyonori Harii, Hirotaka Asato, Kotarou Yoshimura, Atsushi Yamada
    Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 33 (1) 47 - 57 0284-4311 1999 [Not refereed][Not invited]
     
    To acquire symmetry of the cheek when smiling, we carried out 39 free vascularised grafts of the muscle, the motor nerve of which was sutured to a stump of the ipsilateral facial nerve, for 39 patients with facial paralysis. We used two methods: an as healthy and fresh as possible facial nerve stump (method 1A, n = 17) or an incompletely affected stump (method 1B, n = 22). The results are classified into grade 1 to 5 indicating increasing efficiency of muscle function. All patients who had method 1A and 14 patients who had method 1B were evaluated grade 4 or better. Both an incompletely affected facial nerve stump and the proximal stump of a facial nerve that had previously been resected have sufficient function to provide contraction in the grafted muscle.
  • K Yoshimura, T Nakatsuka, S Ichioka, N Kaji, K Harii
    AESTHETIC PLASTIC SURGERY 22 (5) 352 - 355 0364-216X 1998/09 [Not refereed][Not invited]
     
    A one-stage procedure for the reconstruction of a defect of the upper auricle is described. The anterior surface of a carved costal cartilage graft was covered with an anterosuperiorly based skin flap, and the posterior surface was covered by the superficial mastoid fascial flap and a skin graft. This method can be performed easily, without leaving any scar in the hair-bearing area or visible postauricular region, and can be applied to cases in which the condition of the margin scar of an auricular defect is poor.
  • K Yoshimura, T Nakatsuka, S Ichioka, N Kaji, K Harii
    AESTHETIC PLASTIC SURGERY 22 (5) 352 - 355 0364-216X 1998/09 [Not refereed][Not invited]
     
    A one-stage procedure for the reconstruction of a defect of the upper auricle is described. The anterior surface of a carved costal cartilage graft was covered with an anterosuperiorly based skin flap, and the posterior surface was covered by the superficial mastoid fascial flap and a skin graft. This method can be performed easily, without leaving any scar in the hair-bearing area or visible postauricular region, and can be applied to cases in which the condition of the margin scar of an auricular defect is poor.
  • Yoshimura K, Harii K, Asato H, Ueda K, Yamada A
    J Surg Res 79 31 - 38 1998 [Not refereed][Not invited]
  • Yoshimura K, Kuzon WM, Harii K
    J Surg Res 75 135 - 147 1998 [Not refereed][Not invited]
  • Harii K, Asato H, Yoshimura K, Sugawara Y, Nakatsuka T, Ueda K. One-stage transfer of the latissimus dorsi muscle for the reanimation of a paralyzed face
    Plast Reconstr Surg 102 941 - 951 1998 [Not refereed][Not invited]
  • Use of the disposable stapler to insure proper fixation of a transferred muscle in treatment of facial paralysis.
    Asato H, Harii K, Nakatsuka T, Yoshimura K
    J Reconstr Microsurg 19 199 - 204 1998 [Not refereed][Not invited]
  • Use of the disposable stapler to insure proper fixation of a transferred muscle in treatment of facial paralysis.
    Asato H, Harii K, Nakatsuka T, Yoshimura K
    J Reconstr Microsurg 19 199 - 204 1998 [Not refereed][Not invited]
  • Harii K, Asato H, Yoshimura K, Sugawara Y, Nakatsuka T, Ueda K. One-stage transfer of the latissimus dorsi muscle for the reanimation of a paralyzed face
    Plast Reconstr Surg 102 941 - 951 1998 [Not refereed][Not invited]
  • Yoshimura K, Kuzon WM, Harii K
    J Surg Res 75 135 - 147 1998 [Not refereed][Not invited]
  • Yoshimura K, Harii K, Asato H, Ueda K, Yamada A
    J Surg Res 79 31 - 38 1998 [Not refereed][Not invited]
  • Y SUGAWARA, S HIRABAYASHI, H ASATO, K YOSHIMURA
    ANNALS OF PLASTIC SURGERY 34 (1) 78 - 80 0148-7043 1995/01 [Not refereed][Not invited]
     
    Many techniques for reconstruction of an absent umbilicus have been described; however, none has achieved a perfect result. We report a new alternative for constructing an umbilicus using a conical flap and present two representative clinical cases. Our technique creates an umbilicus with sufficient depth with good results, including maintenance of depth after more than 1 year.
  • Influence of skeletal muscle architecture on contractile properties after tenotomy or myotomy.
    Yoshimura K, Faulkner JA, Kuzon WM
    Surgical Forum 46 603 - 605 1995 [Not refereed][Not invited]
  • Influence of skeletal muscle architecture on contractile properties after tenotomy or myotomy.
    Yoshimura K, Faulkner JA, Kuzon WM
    Surgical Forum 46 603 - 605 1995 [Not refereed][Not invited]
  • Y SUGAWARA, S HIRABAYASHI, H ASATO, K YOSHIMURA
    ANNALS OF PLASTIC SURGERY 34 (1) 78 - 80 0148-7043 1995/01 [Not refereed][Not invited]
     
    Many techniques for reconstruction of an absent umbilicus have been described; however, none has achieved a perfect result. We report a new alternative for constructing an umbilicus using a conical flap and present two representative clinical cases. Our technique creates an umbilicus with sufficient depth with good results, including maintenance of depth after more than 1 year.

Awards & Honors

  • 2016/09 American Society of Plastic Surgeons (ASPS) 38th Maliniac Lecture Award
     
    受賞者: Kotaro YOSHIMURA
  • 2015/05 Aesthetic Stem Cell Society Presidential Award
     
    受賞者: Kotaro YOSHIMURA
  • 2015/05 Spanish Society of Cosmetic Medicine and Surgery Doctor Benaprès Award
     
    受賞者: Kotaro YOSHIMURA
  • 2013/04 Japanese Society of Plastic and Reconstructuve Surgery Academic Award
     
    受賞者: Kotaro YOSHIMURA
  • 2012/01 Johnson and Johnson J&J focused funding award
     
    受賞者: 吉村 浩太郎
  • 2011/04 日本形成外科学会 学術奨励賞
     
    受賞者: 吉村 浩太郎
  • 2011/01 IMCAS Award
     
    受賞者: 吉村 浩太郎
  • 2010/10 Prof Illous Award
     
    受賞者: 吉村 浩太郎

Research Grants & Projects

  • 放射線照射による正常組織の確定的組織障害の病態解明と本質的治療法の開発
    文部科学省:科学研究費補助金(挑戦的萌芽研究)
    Date (from‐to) : 2016/04 -2017/03 
    Author : 吉村 浩太郎
  • 文部科学省:科学研究費補助金(基盤研究(B))
    Date (from‐to) : 2015 -2017 
    Author : 吉村 浩太郎
  • 文部科学省:科学研究費補助金(挑戦的萌芽研究)
    Date (from‐to) : 2015 -2015 
    Author : 吉村 浩太郎
  • 文部科学省:科学研究費補助金(基盤研究(B))
    Date (from‐to) : 2012 -2014 
    Author : 吉村 浩太郎
     
    脂肪組織の修復における骨髄前駆細胞の関与を、GFPマウスの移植モデルを使用して調べた。脂肪細胞の再生、血管構築、などに少量の関与が確認され、毛細血管網には大きな関与が認められた。本研究の結果を現在論文投稿用に解析中である。骨髄移植モデルも完成し、その検討をすすめている。モビライザを用いた研究から、各種モビライザの特性について検討をはじめた。骨髄移植は7週齢のマウスに11G照射し4週間おいた後、自家鼠径脂肪移植したものと何もしていないものの比較をした。3ヶ月後(照射後4ヶ月)、ペリリピン染色でGFP陽性の脂肪細胞はどちらも認めなかった。3ヶ月後(照射後4ヶ月)、FACSも行い、ASCは何もしていないもの(放射線障害のみ)8.5%、移植後28.1%のGFP陽性ホスト由来細胞が認められた。骨髄移植したモデルでも照射後4週・6週・6ヶ月、照射4週後に虚血再灌流障害を起こし2週後を観察した。現在結果を解析中である。
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
    Date (from‐to) : 2013 -2013 
    Author : Kotaro YOSHIMURA
     
    SSEA-3+ multipotent mesenchymal cells (Muse cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Muse-rich ASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared to Muse-poor counterpart. Skin ulcers were generated in SCID mice with type 1 diabetes, which showed delayed wound healing compared to non-diabetic SCID mice. Treatment with Muse-rich cells significantly accelerated wound healing compared to treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated epidermis and dermis as epidermal keratinocytes and vascular endothelial cells, respectively. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound healing associated with type 1 diabetes.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
    Date (from‐to) : 2012 -2012 
    Author : Yoshimura KOTARO
     
    Introduction: It remains unclear how ionizing radiation damages healthy skin and hair follicles in a short and long term.Methods: C57BL/J6 mouse (8 weeks old) were distributed to three groups (control: CTR,10Gy once irradiation: 10Gy×1, 5Gy twice irradiations: 5Gy×2). After hair epilation, the groin area was radiated. At 0, 1, 2, and 4 weeks, 3, 6 months after irradiation, the skin samples were evaluated histologically and macroscopically.Results: At 1, 2, and 4 weeks, the epidermis was thicker in 10Gy×1 than CTR and 5Gy×2,without increasing the layer number of epidermal keratinocytes. The number of capillaries in dermis was fewer in 5Gy×2 and 10Gy×1 than CTR at 1 and 2 weeks. At 4 weeks, the the dermis was thicker in CTR than 5Gy×2 and 10Gy×1. At 6 months, the subcutaneous fat tissue was thinner in 5Gy×2 than 10Gy and CTR. At 3 and 6 months, capillaries in groin fat of5Gy×2 and 10Gy×1 appeared to be fewer than thoes of CTR and the size of adipocytes became smaller in 5Gy×2. Many of hair shafts disappeared at 1 week, followed by gradual regrow of hairs by 3 months. The number and size of hair remained decreased in 5Gy×2 and 10Gy×1 than CTR at 6 months when hairs were restored and the gray hairs were macroscopically observed.Discussions/Conclusions: In short term (1 to 4 weeks), the number of hair follicles andcapillaries decreased and the dermis became thinner because of acute radiation injury. In long term (3 to 6 months), tissue damage was generally more severe in 5Gy×2 than 10Gy×1. These data provide clinical insights for minimizing short and long term radiation injuryand revitalizing the radiated ischemic and fibrous tissue.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
    Date (from‐to) : 2010 -2011 
    Author : Kotaro YOSHIMURA, 荒木 淳, Takuya HIGASHINO
     
    Based on the analysis of exudates from injured adipose tissue, we prepared a mixture containing the injury-associated growth factors at the same proportion as the exudates, named adipose injury cocktail(AIC). We hypothesized that AIC induces a series of regenerating and angiogenic processes without actual wounding. The purpose of this study is to elucidate therapeutic potentials of AIC. AIC preferentially activated adipose-derived stem/progenitor cells(ASCs) to proliferate, migrate, and form capillary networks compared to vascular endothelial cells, while VEGF did not induce mitogenesis or chemotaxis in hASCs. Each component growth factor of AIC was differently responsible for the ASC activation. AIC-treated ASCs tended to differentiate into adipocytes or vessel-constituting cells rather than other cell types. In ischemic adipose tissues of mice, induced either by a surgical intervention or by diabetes, AIC administration enhanced proliferation, especially of CD31-/CD34+ASCs, and mitigated tissue hypoxia by increasing capillary density and reducing fibrogenesis. These results suggested that AIC may have therapeutic potentials for various ischemic/hypoxic conditions by inducing adipose remodeling and neovascularization through activation of ASCs and other cells. Treatment with AIC has many advantages over cell-based therapies with regard to morbidity, cost, and physical risks, and may be used for an alternative therapy for improving tissue oxygen tension.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2009 -2011 
    Author : Kotaro YOSHIMURA, 荒木 淳, Takuya HIGASHINO
     
    Many features of adipose stem/progenitor cells, such as physiological functions and localization, have been clarified in the past decade. Adipose tissue turns over very slowly and its perivascular progenitor cells differentiate into new adipocytes, replacing dead adipocytes. A number of clinical trials using freshly isolated or cultured adipose-derived stromal cells(ASCs) containing adipose progenitor/stem cells are ongoing in more than ten countries. Therapeutic use of adipose stem/progenitor cells has been shown to promote angiogenesis and adipose tissue regeneration. Identification of adipocyte releasing factors upon apoptosis/necrosis would be a break-through to step up to the next stage for adipose tissue regeneration. Activation of precursors in perichondrium and periosteum shows a dramatic neogenesis by only a simple injection and an ideal example of in situ tissue engineering. The "hit and catch" strategy using a mobilizer of bone-marrow stem/progenitor cells and a catcher(attractants) to lead the cells to proper homing into the target tissue may be our future direction of promote manipulation of stem cells. Careful design of microenvironment activating ASCs, cell delivery protocol to avoid unexpected behavior and induce maximal potential of ASCs, and selection of target diseases, will be critical to the success of clinical applications.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2007 -2008 
    Author : Kotaro YOSHIMURA, 青井 則之, Koichi GONDA
     
    培養ASCの細胞表面抗原発現の変化 : ASCを接着培養すると新鮮時には発現がなかったCD105(endoglin ; 間葉系幹細胞のマーカーの1つ)が発現することをあきらかにした。また、培養を続けると減少するCD34の発現を長期的に維持する培養法を開発した。ASCは、皮膚線維芽細胞をはじめ、骨髄由来間葉系幹細胞(BM-MSC)、臍帯由来幹細胞など間葉系の組織前駆細胞と形態的には類似しているが、機能、表面抗原発現における相違点を明らかにした。A~dの内容についてJournal of Cellular Physiology誌(2006)に報告した。脂肪再生に関する研究を行い、ヒト吸引脂肪が幹細胞欠乏組織であることを明らかとし、幹細胞を補助的に用いる新しい脂肪移植法の概念を提唱した。免疫不全動物を用いて、ヒト脂肪組織および脂肪組織由来幹細胞を用いて脂肪再生への有効性を示した。また、GFPラットを用いて、幹細胞の追跡実験を行い、血管内皮細胞への分化を確認した。また、ヒト脂肪とヒト脂肪由来幹細胞の混合移植モデル(ヌードマウス)を用いて、やはり血管内皮細胞への分化を確認した。この研究内容については、Tissue Engineering誌(2006)に報告した。遠心処理により脂肪組織から水分が失われ、一部の脂肪細胞は破壊されるが幹細胞は生存することを明らかにし、また脂肪移植における遠心処理の最適化に関する研究についてPlast Reconstr Surg誌(2008)に報告した。脂肪組織および幹細胞の非凍結保存の最適化についてPlast Reconstr Surg誌(2007)に報告するとともに、長期凍結保存した脂肪由来幹細胞の機能解析結果をやはりPlast ReconstrSurg誌(2008)に報告した。脂肪幹細胞の多分化能を維持したままで、効率的に短期間で増殖させる方法についてCytotherapy誌(2007)に報告した。脂肪由来幹細胞にbFGFが作用するとHGFを強力にHGFを分泌することを明らかにした。またこの作用はJNKシグナルを介し、脂肪組織の虚血再還流傷害後の脂肪再生、血管新生において、脂肪幹細胞由来のHGFが線維化を抑制する働きがあることを明らかにした。また、血管新生、脂肪再生において脂肪幹細胞が主役的な役割を果たして増殖、遊走することを明らかにした。これらの結果はStem Cells誌(2008)に報告した。
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2005 -2006 
    Author : 岡崎 睦, Kotaro YOSHIMURA, 吉村 浩太郎, Takashi NAGASE
     
    Dermal papilla cells (DPCs) in the mammalian hair follicles have been shown to have an ability to induce and develop hair follicles. It is theoretically possible to regenerate human hair by expanding DPCs and transplanting them in the bald skin.We performed a microarray analysis and RT-PCR comparing human dermal papilla cells and dermal fibroblasts both obtained from adult scalp skin, in order to elucidate the genetic factors responsible for hair induction in tissue engineered hair follicles. In the microarray analysis we found that TGFs gene was significantly upregulated compared to dermal fibroblasts and that it was one of the candidate genes involving in hair regeneration. RT-PCR analysis revealed significant and sustained upregulation of TGFs mRNA in cultured dermal papilla cells compared to dermal fibroblasts. Furthermore, we found soluble factors secreted by keratinocytes significantly upregulated TGF beta-2 mRNA.Immunohistochemistry (IHC) of human scalp skin and hair follicles for the biomarkers previously reported as specific for IFKC was performed. The expression of the biomarkers in cultured keratinocytes was also investigated by FACS analysis and immunostaining. Keratin 15 (K15) was expressed in the bulge area in IHC. CD34 was expressed in the lower area, which includes the sub-bulge area and outer root sheath (ORS). CD271 (p75 neurotrophin receptor) was expressed in the lower ORS that was negative for K15. All of them were expressed only in the outermost layer adhering to the basement membrane.We examined reproducibility of two of in vivo models for trichogenesis previously reported, including 'chamber models', grafting cultured human DPCs into silicone chambers on nude mice, and 'sandwich models', implanting cultured human DPCs with rat sole skin under the skin of nude mice. 'Sandwich models' were more useful than 'chamber models' and other in vivo models for testing the inductive viability of human DPCs in terms of reproducibility.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2004 -2005 
    Author : Kotaro YOSHIMURA, Mustumi OKAZAKI, Takashi NAGASE
     
    Liposuction aspirates (primarily saline solution, blood, and adipose tissue fragments) separate into fatty and fluid portions. Cells isolated from the fatty portion are termed processed lipoaspirate (PTA) cells and contain adipose-derived adherent stromal cells (ASCs). Here we define cells isolated from the fluid portion of liposuction aspirates as liposuction aspirate fluid (LAF) cells. Stromal vascular fractions (SVF) were isolated separately from both portions and characterized under cultured and non-cultured conditions. A comparable number of LAF and PLA cells were freshly isolated, but fewer LAF cells were adherent. CD34+CD45- cells from fresh LAF isolates were expanded by adherent culture, suggesting that LAF cells contain ASCs. Although freshly isolated PLA and LAF cells have distinct cell surface marker profiles, adherent PLA and LAF cells have quite similar characteristics with regard to growth kinetics, morphology, capacity for differentiation, and surface marker profiles. After plating, both PLA and LAF cells showed significant increased expression of CD29, CD44, CD49d, CD73, CD90, CD105,and CD151 and decreased expression of CD31 and CD45. Multicolor FACS analysis revealed that SVF are composed of heterogeneous cell populations including blood-derived cells (CD45+), ASCs (CD31-CD34+CD45-CD90+CD105-CD146-), endothelial (progenitor) cells (CD31+CD34+CD45-CD90+CD105lowCD146+), pericytes (CD31-CD34-CD45-CD90+CD105-CD146+), and other cells. After plating, ASCs showed a dramatic increase in CD105 expression. Although some adherent ASCs lost CD34 expression with increasing culture time, our culture method maintained CD34 expression in ASCs for at least 10-20 weeks. These results suggest that liposuction-derived cells may be useful and valuable for cell-based therapies.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2002 -2003 
    Author : Kotaro YOSHIMURA, 加地 展之, 多久嶋 亮彦, Mutsumi OKAZAKI, Takashi NAGASE
     
    Keloids are skin abnormalities that are characterized by excessive deposition of collagen bundles in the dennis. Patients with keloids complain not only about their cosmetic appearances, but also about continuous itching andlor tenderness associated with chronic inflammation. Degradation of extracellular matrix (ECM) may be upregulated associated with the expansion of keloids into circumferential skin, and high metabolic activity of keloid tissues may be due to increased matrix metalloproteinases (MMPs) activity. Based on these hypotheses, we examined differences in expressions of MMP-1, MMP-8, and MMP-13 between keloid-derived fibroblasts and normal dermal fibroblasts. Since retinoids are potent inhibitors of MMPs in the treatment of photoaged skin and cancers, we also examined whether or not tretinoin affects MMPs expressions of keloid-derived fibroblasts.The results of real-time PCR and ELISA demonstrated a significant upregulation of MMP-13 as well as significant downregulation of MMP-1 and MMP-8 in keloid-derived fibroblasts, both at mRNA and protein levels. MMP-1 mRNA expression in the control group was significantly upregulated after the addition of tretinoin, whereas no significant change was observed in the keloid group : MMP-8 mRNA expression in the control group was significantly upregulated with the peak at 12 hours by tretinoin, while no significant change was observed in the keloid-derived fibroblasts. In contrast, the remarkably elevated MMP-13 mRNA expression in the keloid group was significantly suppressed with the peak suppression at 12 hours after. addition of tretinoin, while MMP-13 mRNA expression in the control group was not significantly changed.The decrease in MMP-1 and MMP-8 may contribute to accumulation of type I and type III collagen in keloid tissues, and this mechanism may be modulated by molecular interaction with MMP-13. Tretinoin appeared to reverse the abnormal expression profile of MMPs in keloid-derived fibroblasts, such as markedly elevated expression of MMP-13, partly through inactivation of AP-1 pathway. The present results suggested that tretinoin may be clinically useful to improve chronic inflammation seen in keloids and prevent expansion of keloid tissues into circumferential normal skin.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2000 -2001 
    Author : Kotaro YOSHIMURA, Takao AOYAMA, 北野 幸恵, Mutsumi OKAZAKI, Yukie KITANO
     
    The results suggested that RA neither have direct inhibitory effects on melanogenesis of melanocytes, nor influence the cell-cell interactions between melanocytes, keratinocytes and fibroblasts, such as paracrine actions with regard to melanin productions. The role of RA in bleaching treatments appears to be other specific actions, such as promotion of keratinocytes proliferation and acceleration of epidermal turnover. The results also revealed that HB-EGF mRNA expression was elevated dose-dependently with atRA, most at 12 hours. This elevation was more prominent in keratinocytes of confluent state than those of subconfluent state, suggesting differentiated keratinocytes are more subject to be stimulated by atRA with regard to HB-EGF expression than proliferating keratinocytes. Ral and Rol was found to upregulate HB-EGF when used at 100 μM to 1mM, to a similar extent of atRA at 1 to 10 μM. The capacity of retinoids to upregulate HB-EGF may be an important index to develop and seek an ideal synthetic retinoid, which has maximum benefits and minimal side effects.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 1998 -1999 
    Author : 中塚 貴志, Kotaro YOSHIMURA, Koichi GONDA, Takashi NAKATSUKA
     
    In vivo gene transfer is a recently-developed device for efficient delivery of a therapeutic recombinant protein. We formulated the hypothesis that a high level of expression of bone morphogenetic protein-2 (BMP-2) could be a future therapeutical modality in terms of inducing substantial bone formation in vivo. First to test this hypothesis, adenoviruses carrying BMP-2 gene were directly injected into the soleus muscle of adult rat. The BMP-2 gene was successfully overexpressed in the target muscle by adenovirus-mediated transfer, whereas bone formation in and around the muscle failed to occur in this case. Secondly, in order to recruit putative osteoprogenitor cells, we then induced ischemic degeneration of the target muscle by orthotopically grafting it simultaneously with the gene transfer. The combination of BMP-2 gene transfer and orthotopic muscle grafting resulted in successful ossification of almost the whole grafted muscle, whereas neither muscle grafting alone nor the combination of muscle grafting and adenovirus-mediated transfer of reporter gene, LacZ induced any bone formation in the muscle. The ossification process was evident by positive von Kossa staining of the histological sections and roentogenographical radio-opacity of the region. It was also found that the BMP-2 transgene overexpressed in grafted muscles inhibited muscle regeneration, which should otherwise follow the muscle degeneration. We further demonstrated an upregulation of BMP receptor type IA in grafted muscles, suggesting its involvement in the bone formation process. In conclusion, overexpression of BMP-2 gene induced massive heterotopic ossification in skeletal muscles under graft-induced ischemic degeneration, which possibly upregulates osteoprogenitor cells in situ.
  • Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 1996 -1997 
    Author : Kotaro YOSHIMURA, 水尾 弘司, 浅谷 倫代, 川崎 裕史, 鈴木 康俊, Yasutoshi SUZUKI, Yushi KAWASAKI
     
    The purpose of this study is to examined the cellular and molecular events coincident with muscle denervation, especially a regenerative change seen following muscle denervation, the role of satellite cells involved in the process, and the possibility of apoptotic degeneration of myonuclei as a mechanism of myonuclei loss during muscle denervation atrophy. Myosin heavy chain (MHC) isoform expression during muscle denervation was examined using pyrophosphate acrylamide gel electrophoresis and immunohistochemistry. DNA fragmentation (apoptosis) in myonuclei of denervated fibers was investigated using agarose gel electrophoresis, the TUNEL technique and ELISA.Immunohistchemistry for MyoD and BrdU,and an ultrastructural survey were also performed. Following muscle denervation, embryonic MHC which is not expressed in adult healthy muscles was expressed in some denervated fibers as well as small activated satellite cells ; maximal expression was observed two to three weeks after denervation. Activation and proliferation of satellite cells were observed, while few typical regenerating fibers were identified, suggesting that activated satellite cells fused to the denervated maternal fibers to try to repair them instead of fusing to each other to form ner fibers, likely as a mechanism to compensate for atrophic changes after denervation. DNA fragmentation was detected by the TUNEL technique and ELISA,although DNA ladder formation was not observed with agarose gel electrophoresis. Apoptotic degeneration of myonuclei of denervated fibers was not confirmed, but it may contribute to loss of myoneuclei associated with denervation atrophy and induce activation of satellite cells.
  • 文部科学省:科学研究費補助金(一般研究(C))
    Date (from‐to) : 1994 -1994 
    Author : 吉村 浩太郎, 川嶋 孝雄, 中川 雅裕
     
    われわれはコリンエステラーゼ染色および炭酸脱水酵素染色を用いて、ヒトおよびラットの末梢神経線維の標識を行ない、現在までのところ次のような結果を得た。ラットにおいては、コリンエステラーゼ染色は遠心性線維のみを標識し、神経線維の識別に有用であった。一方、炭酸脱水酵素染色においては大半の求心性線維を標識するものの、一部の皮膚感覚神経線維は陰性であった。しかし固有感覚神経線維についてはすべて陽性であり、とくに筋肉神経における識別には有用と考えられる。これらの染色法においては染色性が前固定により強く左右され、前者は未固定を好み後者はグルタルアルデヒドなどによる強力な固定が必要なため、二重染色には中間的な特殊な固定法が必要となり、可能ではあったが双方とも染色性をやや犠牲にするという難点が明らかになった。ヒトにおいては、炭酸脱水酵素染色はラットとほぼ同様の結果であっが、コリンエステラーゼ染色においては一部の無髄自律神経線維が強く陽性を示すため、それらを多く含む皮膚神経においては強拡大による詳細な観察を必要とした。再生神経についてはその線維組織や構造の乱れから超薄切片による観察が必要であった。神経断端に生じる神経腫についても、再生神経においても初期においては求心性線維の成長が優位であることが観察された。
  • Research on retinoids for rejuvenation and cosmesis of the skin
  • Cell-based regenerative medicine


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