Researchers Database

kamioka masashi

    CardiovascularCenter,InternalMedicine Assistant Professor
Contact: kmasashijichi.ac.jp
Last Updated :2021/10/17

Researcher Information

J-Global ID

Research Areas

  • Life sciences / Cardiology

Education

  •        - 2006  Fukushima Medical University  医学研究科
  •        - 2006  Fukushima Medical University  Graduate School, Division of Medicine

Published Papers

  • Yoshiyuki Matsumoto, Takashi Kaneshiro, Naoko Hijioka, Minoru Nodera, Shinya Yamada, Masashi Kamioka, Akiomi Yoshihisa, Hiroshi Ohkawara, Takuto Hikichi, Hitoshi Suzuki, Yasuchika Takeishi
    Journal of Interventional Cardiac Electrophysiology 54 (2) 101 - 108 2019/03 [Refereed][Not invited]
  • Takashi Kaneshiro, Yoshiyuki Matsumoto, Naoko Hijioka, Minoru Nodera, Shinya Yamada, Masashi Kamioka, Akiomi Yoshihisa, Hiroshi Ohkawara, Hitoshi Suzuki, Yasuchika Takeishi
    JACC:Clinical Electrophysiology 4 (12) 1642 - 1643 2018/12 [Refereed][Not invited]
  • Shinya Yamada, Akiomi Yoshihisa, Yu Sato, Takamasa Sato, Masashi Kamioka, Takashi Kaneshiro, Masayoshi Oikawa, Atsushi Kobayashi, Hitoshi Suzuki, Takafumi Ishida, Yasuchika Takeishi
    Journal of Cardiovascular Electrophysiology 29 (9) 1257 - 1264 2018/09 [Refereed][Not invited]
  • Takashi Kaneshiro, Yoshiyuki Matsumoto, Minoru Nodera, Masashi Kamioka, Yoshiyuki Kamiyama, Akiomi Yoshihisa, Hiroshi Ohkawara, Hitoshi Suzuki, Yasuchika Takeishi
    Europace 20 (7) 1122 - 1128 2018/07 [Refereed][Not invited]
  • Minoru Nodera, Hitoshi Suzuki, Yoshiyuki Matsumoto, Masashi Kamioka, Takashi Kaneshiro, Akiomi Yoshihisa, tetsuya Ohira, Yasuchika Takeishi
    Cardiology 140 (1) 47 - 51 2018/05 [Not refereed][Not invited]
  • Takashi Kaneshiro, Hitoshi Suzuki, Minoru Nodera, Shinya Yamada, Masashi Kamioka, Yoshiyuki Kamiyama, Yasuchika Takeishi
    PACE-PACING AND CLINICAL ELECTROPHYSIOLOGY 39 (4) 338 - 344 0147-8389 2016/04 [Refereed][Not invited]
     
    BackgroundIn catheter ablation of idiopathic ventricular arrhythmia (VA), it is still unclear whether pace mapping or activation mapping is more useful for successful catheter ablation. The depth of origin in the ventricular wall especially affects the success rate of endocardial-approached catheter ablation. Thus, we examined the relationship between these tactics and QRS morphology. MethodsWe evaluated the relationship among pace mapping score, activation time, and peak deflection index (PDI) in 28 patients, with a total of 30 origins, who underwent successful catheter ablation of idiopathic VA. ResultsAll origins were located in the ventricular outflow tract area, including three in the left coronary cusp (LCC). PDI, activation time, and pace mapping score at successful ablation sites were 0.60 0.08, 26.3 +/- 9.9 ms, and 19.1 +/- 4.6, respectively. The pace mapping score inversely correlated with the PDI (R = -0.540, P = 0.0017), but the activation time did not correlate with the PDI. When excluding the three VAs originating from the LCC, in which perfect pace mapping was obtained from epicardial sites despite high PDI, this correlation coefficient became more intensive (R = -0.734, P < 0.0001). ConclusionsOur study suggests that pace mapping with an endocardial approach could not reproduce the precise QRS morphology for VA originating from the intramural site of the ventricular wall. With such origins, we should rely on activation mapping to detect the optimal ablation site.

Conference Activities & Talks

  • 2枝慢性完全閉塞病変へのドナー冠動脈に急性心筋梗塞を発症し、梗塞責任病変および慢性完全閉塞病変を一期的治療し救命し得た一例  [Not invited]
    第39回 日本心血管インターベンション治療学会東北地方会  2016
  • 弾性線維性仮性黄色腫に合併した重症二枝冠動脈病変に対して完全血行再建を施行した一例  [Not invited]
    第39回 日本心血管インターベンション治療学会東北地方会  2016
  • Association of ratio of eicosapentaenoic acid to arachidonic acid with T wave alternans in patients with ischemic heart disease  [Not invited]
    第79回 日本循環器学会学術集会  2015
  • Impact of hypocalcemia on mortality of patients with heart failure and chronic kidney disease  [Not invited]
    第79回 日本循環器学会学術集会  2015
  • Impact of hypertension on anatomical change of left atrial wall in patients with atrial fibrillation  [Not invited]
    第79回 日本循環器学会学術集会  2015
  • 経静脈的心房腫瘤生検によって確定診断をつけることができた血管肉腫の一例  [Not invited]
    第160 回 日本循環器学会東北地方会  2015
  • ボセンタンが繰り返す失神に対して著効した慢性腎不全を合併する肺動脈性肺高血圧症の1 例  [Not invited]
    第160 回 日本循環器学会東北地方会  2015
  • 陳旧性心筋梗塞に合併したPVC に対するアブレーションが有用だった1例  [Not invited]
    第160 回 日本循環器学会東北地方会  2015
  • 両心室からのアブレーションが有用であったと考えられた心室頻拍の1例  [Not invited]
    第12回 福島不整脈懇話会  2015
  • Circadian variation of ventricular tachycardia in cardiomyopathy was associated with instability of repolarization  [Not invited]
    第30回 日本不整脈学会学術大会, 32回 日本心電学会学術集会 合同学術大会  2015
  • The existence of high defibrillation threshold indicates the incidence of pneumothorax during cardiac resynchronization therapy defibrillator implantation  [Not invited]
    第30回 日本不整脈学会学術大会, 32回 日本心電学会学術集会 合同学術大会  2015
  • Reverse S curveを用いて治療し得たLVOT summit起源心室頻拍の一例  [Not invited]
    第7回 東北臨床不整脈研究会学術集会  2015
  • ボセンタンが著効した慢性腎不全が原因と考えられる肺動脈性肺高血圧症の1例  [Not invited]
    第63回 日本心臓病学会学術集会  2015
  • 左心房前壁天井側に存在する瘢痕を旋回した心房頻拍の1例  [Not invited]
    カテーテルアブレーション関連秋季大会2015  2015
  • 上大静脈起源との判別に微小肺静脈内電位が重要な所見であった、右上肺静脈起源心房頻拍の1例  [Not invited]
    カテーテルアブレーション関連秋季大会2015  2015
  • 弾性線維性仮性黄色腫に合併した重症二枝冠動脈病変に対して完全血行再建を施行し得た一例  [Not invited]
    第161回 日本循環器学会東北地方会  2015
  • Association of ratio of eicosapentaenoic acid to arachidonic acid with T wave alternans in patients with ischemic heart disease  [Not invited]
    The 79th Annual Scientific Meeting of the Japanese Circulation Society  2015
  • Impact of hypocalcemia on mortality of patients with heart failure and chronic kidney disease  [Not invited]
    The 79th Annual Scientific Meeting of the Japanese Circulation Society  2015
  • Impact of hypertension on anatomical change of left atrial wall in patients with atrial fibrillation  [Not invited]
    The 79th Annual Scientific Meeting of the Japanese Circulation Society  2015
  • Circadian variation of ventricular tachycardia in cardiomyopathy was associated with instability of repolarization  [Not invited]
    2015
  • The existence of high defibrillation threshold indicates the incidence of pneumothorax during cardiac resynchronization therapy defibrillator implantation  [Not invited]
    2015
  • Electrocardiographic and electrophysiological predictors of ventricular arrhythmias originating from the left ventricular outflow tract above and below aortic valve  [Not invited]
    Heart Rhythm 2014, 36th Annual Scientific Sessions  2014
  • Electrocardiographic and electrophysiological predictors of ventricular arrhythmias originating from the left ventricular outflow tract within and below the coronary sinus cusp  [Not invited]
    Congress of the EHRA Europace-Cardiostim 2014  2014
  • カテーテルアブレーションによりVT/VF stormから脱却し得た左室緻密化障害の1例  [Not invited]
    第11回 福島不整脈懇話会  2014
  • 左脚後枝領域Purkinje network起源のVF triggerと心外膜起源VTへのアブレーションによりVT/VF stormから脱却し得た左室緻密化障害の1例  [Not invited]
    第26回 カテーテルアブレーション委員会公開研究会  2014
  • 糖尿病性腎症に合併した左房前壁天井側に存在する瘢痕を旋回した心房頻拍の一例  [Not invited]
    第159回 日本循環器学会東北地方会  2014
  • 5Fアブレーションカテーテルが有効だったCRT-D植え込み後の心外膜起源心室頻拍の1例  [Not invited]
    第159回 日本循環器学会東北地方会  2014
  • Electrocardiographic and electrophysiological predictors of ventricular arrhythmias originating from the left ventricular outflow tract above and below aortic valve  [Not invited]
    Heart Rhythm 2014, 36th Annual Scientific Sessions  2014
  • Electrocardiographic and electrophysiological predictors of ventricular arrhythmias originating from the left ventricular outflow tract within and below the coronary sinus cusp  [Not invited]
    Congress of the EHRA Europace-Cardiostim 2014  2014
  • ARDS により不幸な転機をたどった薬物治療抵抗性感染性心内膜炎の1例  [Not invited]
    第156回 日本循環器学会東北地方会  2013
  • Can transseptal puncture be safety performed in patients following implantation of a closure device for atrial septal defect?  [Not invited]
    Congress of the European Society of Cardiology 2013  2013
  • Electrophysiological characteristics and acute success of catheter ablation of ventricular arrhythmia originating from the Papillary Muscles  [Not invited]
    Congress of German Association of Cardiology 2013  2013
  • Can transseptal puncture be safety performed in patients following implantation of a closure device for atrial septal defect?  [Not invited]
    Congress of the European Society of Cardiology 2013  2013
  • Electrophysiological characteristics and acute success of catheter ablation of ventricular arrhythmia originating from the Papillary Muscles  [Not invited]
    Congress of German Association of Cardiology 2013  2013
  • The enormous earthquake hit Japan on March 11 increased acute heart failure  [Not invited]
    第76回 日本循環器学会学術集会  2012
  • A novel practical strategy for prediction of worsening heart failure by intrathoracic impedance  [Not invited]
    第76回 日本循環器学会学術集会  2012
  • High-sensitivity C-reactive protein predicts poor response to cardiac resynchronization therapy and adverse prognosis in patients with chronic heart failure  [Not invited]
    第76回 日本循環器学会学術集会  2012
  • Sleep-disordered breathing induces cardiac electrical instability associated with life-threatening ventricular arrhythmias in patients with chronic heart failure  [Not invited]
    第76回 日本循環器学会学術集会  2012
  • T-wave alternans is useful for predicting adverse outcomes in ventricular fibrillation survivors  [Not invited]
    第76回 日本循環器学会学術集会  2012
  • Sleep-disordered breathing impairs ventricular reporalization and autonomic nervous system associated with life-threatening arrhythmias in patients with chronic heart failure  [Not invited]
    Heart Rhythm 2012, 33rd Annual Scientific Sessions  2012
  • 留置後10 年経過したベアメタルステント内に発生した不安定プラークの破綻により急性冠症候群を発症した1 例(YIA)  [Not invited]
    第154 回 日本循環器学会東北地方会  2012
  • CRT 植え込みにて僧帽弁逆流が改善した陳旧性心筋梗塞の一例  [Not invited]
    第154 回 日本循環器学会東北地方会  2012
  • 僧帽弁輪起源の心室頻拍に対してアブレーションを行った一例  [Not invited]
    第154 回 日本循環器学会東北地方会  2012
  • リモートモニタリングの活用:震災時におけるリモートモニタリング活用の有用性と問題点  [Not invited]
    第27回 日本不整脈学会学術大会  2012
  • 心室細動蘇生例におけるT wave alternans 測定の意義について  [Not invited]
    第27回 日本不整脈学会学術大会  2012
  • 拡張型心筋症における左室拡張能障害と致死性不整脈出現の関連について  [Not invited]
    第27回 日本不整脈学会学術大会  2012
  • 無呼吸検出モニター付きICD の有用性を検討しえた1 例  [Not invited]
    第27回 日本不整脈学会学術大会  2012
  • A case report that distal protection using two filtraps to the true bifurcation lesion was effective  [Not invited]
    第21回 日本心血管インターベンション治療学会・学術集会  2012
  • A case of acute coronary syndrome caused by in-stent vulnerable plaque rupture at ten years after implantation of BMS  [Not invited]
    第21回 日本心血管インターベンション治療学会・学術集会  2012
  • The enormous earthquake hit Japan on March 11 increased acute heart failure -analysis of remote monitoring of intrathoracic impedance-  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • The association between sleep-disordered breathing and life-threatening ventricular arrhythmias in patients with chronic heart failure  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • T-wave alternans is helpful for predicting recurrence of fatal arrhythmias in ventricular fibrillation survivors  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • CRTデバイス植込み前の高感度CRPは心臓死の予後予測因子となりうるか  [Not invited]
    第29回 日本心電学会学術集会  2012
  • The enormous earthquake hit Japan on March 11 increased acute heart failure  [Not invited]
    The 76th Annual Scientific Meeting of the Japanese Circulation Society  2012
  • A novel practical strategy for prediction of worsening heart failure by intrathoracic impedance  [Not invited]
    The 76th Annual Scientific Meeting of the Japanese Circulation Society  2012
  • High-sensitivity C-reactive protein predicts poor response to cardiac resynchronization therapy and adverse prognosis in patients with chronic heart failure  [Not invited]
    The 76th Annual Scientific Meeting of the Japanese Circulation Society  2012
  • Sleep-disordered breathing induces cardiac electrical instability associated with life-threatening ventricular arrhythmias in patients with chronic heart failure  [Not invited]
    The 76th Annual Scientific Meeting of the Japanese Circulation Society  2012
  • T-wave alternans is useful for predicting adverse outcomes in ventricular fibrillation survivors  [Not invited]
    The 76th Annual Scientific Meeting of the Japanese Circulation Society  2012
  • Sleep-disordered breathing impairs ventricular reporalization and autonomic nervous system associated with life-threatening arrhythmias in patients with chronic heart failure  [Not invited]
    Heart Rhythm 2012, 33rd Annual Scientific Sessions  2012
  • A case report that distal protection using two filtraps to the true bifurcation lesion was effective  [Not invited]
    2012
  • A case of acute coronary syndrome caused by in-stent vulnerable plaque rupture at ten years after implantation of BMS  [Not invited]
    2012
  • The enormous earthquake hit Japan on March 11 increased acute heart failure -analysis of remote monitoring of intrathoracic impedance-  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • The association between sleep-disordered breathing and life-threatening ventricular arrhythmias in patients with chronic heart failure  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • T-wave alternans is helpful for predicting recurrence of fatal arrhythmias in ventricular fibrillation survivors  [Not invited]
    Congress of the European Society of Cardiology 2012  2012
  • The assessment of circadian variation of late potential is useful for predicting the occurrence of ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    Heart Rhythm 2011, 32nd Annual Scientific Sessions  2011
  • The relation of the decreasing rates of intrathoracic impedance and decompensated heart failure  [Not invited]
    Heart Rhythm 2011, 32nd Annual Scientific Sessions  2011
  • 留置後10年経過したベアメタルステント内に不安定プラークが形成され、急性冠症候群を発症した一例  [Not invited]
    第67回 福島循環器談話会  2011
  • 心室細動を来した若年女性の3症例  [Not invited]
    第8回 福島不整脈懇話会  2011
  • Case report that guide wire and Corsair were broken, when guide wire located outside stent was pulled using Corsair  [Not invited]
    第20回 日本心血管インターベンション治療学会  2011
  • Adaptive servo-ventilation improves cardiac function and prognosis in heart failure patients with Cheyne-Stokes respiration after cardiac resynchronization therapy  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • Changes in intrathoracic impedance is related to left ventricular reverse remodeling after CRT-D implantation in chronic heart failure  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • Circadian variation of late potential predicts the occurrence of ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • Preventive effect of chronic endothelin type A receptor antagonist on coronary microvascular spasm induced by epicardial coronary artery endothelial denudation  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • Repeated late potential analysis by 24-hr Holter monitoring is useful for predicting ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) is a Novel Multifunctional Modifier for Vascular Responses  [Not invited]
    第75回 日本循環器学会学術集会  2011
  • CRT後残存したチェーンストーク呼吸に対するASV導入効果  [Not invited]
    第2回 東北心不全研究会  2011
  • 植え込み型デバイスからみた東日本大震災  [Not invited]
    第3回 東北臨床不整脈研究会  2011
  • Adaptive servo ventilation improves not only sympathetic function but also physical performance: results from new multifunction wireless Holter ECG  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Association between characteristics of left ventricular pacing lead and reverse remodeling in response to cardiac resynchronization therapy  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Long-term increase of intrathoracic impedance after cardiac resynchronization therapy is associated with left ventricular reverse remodeling  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Monitoring of changes in intrathoracic impedance is useful for predicting decompensated heart failure  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Adaptive servo ventilationは交感神経活性を抑制する:多機能ワイヤレスホルター心電計による検討  [Not invited]
    第5回 東北Heart & Sleep研究会  2011
  • CARTOを用いた右房前中隔のマッピングにより無冠尖起源の心房頻拍が疑われ根治に成功した一例  [Not invited]
    第23回 カテーテル・アブレーション委員会公開研究会  2011
  • Adaptive servo ventilation improves cardiac function and prognosis in chronic heart failure patients with Cheyne-Stokes respiration after cardiac resynchronization therapy  [Not invited]
    Scientific Sessions of American Heart Association 2011  2011
  • 慢性心不全患者において睡眠呼吸障害の合併は致死性不整脈発生の基盤を形成する  [Not invited]
    第153回 日本循環器学会東北地方会  2011
  • 心室細動にて発症した若年女性の左室緻密化障害の一例  [Not invited]
    第153回 日本循環器学会東北地方会  2011
  • The assessment of circadian variation of late potential is useful for predicting the occurrence of ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    Heart Rhythm 2011, 32nd Annual Scientific Sessions  2011
  • The relation of the decreasing rates of intrathoracic impedance and decompensated heart failure  [Not invited]
    Heart Rhythm 2011, 32nd Annual Scientific Sessions  2011
  • Case report that guide wire and Corsair were broken, when guide wire located outside stent was pulled using Corsair  [Not invited]
    2011
  • Adaptive servo-ventilation improves cardiac function and prognosis in heart failure patients with Cheyne-Stokes respiration after cardiac resynchronization therapy  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Changes in intrathoracic impedance is related to left ventricular reverse remodeling after CRT-D implantation in chronic heart failure  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Circadian variation of late potential predicts the occurrence of ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Preventive effect of chronic endothelin type A receptor antagonist on coronary microvascular spasm induced by epicardial coronary artery endothelial denudation  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Repeated late potential analysis by 24-hr Holter monitoring is useful for predicting ventricular tachycardia in patients with dilated cardiomyopathy  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) is a Novel Multifunctional Modifier for Vascular Responses  [Not invited]
    The 75th Annual Scientific Meeting of the Japanese Circulation Society  2011
  • Adaptive servo ventilation improves not only sympathetic function but also physical performance: results from new multifunction wireless Holter ECG  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Association between characteristics of left ventricular pacing lead and reverse remodeling in response to cardiac resynchronization therapy  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Long-term increase of intrathoracic impedance after cardiac resynchronization therapy is associated with left ventricular reverse remodeling  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Monitoring of changes in intrathoracic impedance is useful for predicting decompensated heart failure  [Not invited]
    The 4th Asia Pacific Heart Rhythm Society(APHRS) Scientific Session, 第26回 日本不整脈学会,第28 回 日本心電学会, 合同学術集会  2011
  • Adaptive servo ventilation improves cardiac function and prognosis in chronic heart failure patients with Cheyne-Stokes respiration after cardiac resynchronization therapy  [Not invited]
    Scientific Sessions of American Heart Association 2011  2011
  • Pivotal role of receptor for advanced glycation end products (RAGE) in hyperlipidemia-dependent atherosclerosis and molecular regulation  [Not invited]
    第74回 日本循環器学会学術集会  2010
  • 血管内皮細胞機能障害と活性酸素種産生に対するプラバスタチン静脈内投与の効果とその機序  [Not invited]
    第13回 福島動脈硬化Forum  2010
  • Pivotal role of receptor for advanced glycation end products (RAGE) in hyperlipidemia-dependent atherosclerosis and molecular regulation  [Not invited]
    20th World Congress of the International Society for Heart Research (ISHR)  2010
  • Preventive effects of pravastatin on thrombin-triggered vascular responses mediated via Akt/eNOS and RhoA/Rac1 signaling pathways in vivo in rats  [Not invited]
    第42回 日本動脈硬化学会総会・学術集会  2010
  • LADのCTOに対する逆行性アプローチのルートとして、#4PDから対角枝に繋がるepicardila channelを使用した一例  [Not invited]
    第33回 Coronary Intervention Conference (CIC)  2010
  • 心室細動発症に不整脈基質の関与が考えられた冠攣縮性狭心症の一例  [Not invited]
    第151回 日本循環器学会東北地方会  2010
  • 右冠動脈から対角枝への心外膜側側副血行路を介して左前下行枝慢性閉塞病変に対しPCIを施行した1例  [Not invited]
    第151回 日本循環器学会東北地方会  2010
  • カテーテルアブレーション翌日に根治が得られた反復性心室頻拍の一例  [Not invited]
    第151回 日本循環器学会東北地方会  2010
  • Pivotal role of receptor for advanced glycation end products (RAGE) in hyperlipidemia-dependent atherosclerosis and molecular regulation  [Not invited]
    The 74th Annual Scientific Meeting of the Japanese Circulation Society  2010
  • Pivotal role of receptor for advanced glycation end products (RAGE) in hyperlipidemia-dependent atherosclerosis and molecular regulation  [Not invited]
    20th World Congress of the International Society for Heart Research (ISHR)  2010
  • Preventive effects of pravastatin on thrombin-triggered vascular responses mediated via Akt/eNOS and RhoA/Rac1 signaling pathways in vivo in rats  [Not invited]
    2010
  • Involvement of membrane type 1-matrix metalloproteinase (MT1-MMP) in advanced glycation end products (AGE)/RAGE-signaling pathway  [Not invited]
    第73回 日本循環器学会学術集会  2009
  • Rapid improvement of impaired endothelium-dependent vasodilation and Rac1/NADPH oxidase-derived ROS generation in vivo by pravastatin  [Not invited]
    第73回 日本循環器学会学術集会  2009
  • 血管内皮細胞機能不全における酸化LDL誘導RhoA・Rac1活性化とLOX-1/MT1-MMP系の役割  [Not invited]
    第12回 福島動脈硬化Forum  2009
  • Crucial role of unprocessed RhoA and Rac1 in progression of atherosclerosis in Watanabe heritable hyperlipidemic (WHHL) Rabbits  [Not invited]
    第41回 日本動脈硬化学会学術総会  2009
  • Integral role of receptor for advanced glycation end products (RAGE) in non-diabetic atherosclerosis  [Not invited]
    第41回 日本動脈硬化学会学術総会  2009
  • Membrane type matrix metalloproteinase-1 (MT1-MMP) is a cell surface modifier of RhoA/Rac1-mediated signaling pathways and forms a complex with LOX-1 in oxidized LDL-induced endothelial dysfunction  [Not invited]
    Congress of the European Society of Cardiology 2009  2009
  • 内皮細胞への単球接着系でのレドックス、Akt/eNOSおよびカルシウムシグナル伝達における酸化LDL受容体LOX-1の役割  [Not invited]
    第47回 東北止血・血栓研究会  2009
  • Cooperation of MT1-MMP and LOX-1 for RhoA- and Rac1-dependent signaling pathways in oxidized LDL-mediated endothelial dysfunction and atherosclerosis  [Not invited]
    Scientific Sessions of American Heart Association 2009  2009
  • Involvement of membrane type 1-matrix metalloproteinase (MT1-MMP) in advanced glycation end products (AGE)/RAGE-signaling pathway  [Not invited]
    The 73rd Annual Scientific Meeting of the Japanese Circulation Society  2009
  • Rapid improvement of impaired endothelium-dependent vasodilation and Rac1/NADPH oxidase-derived ROS generation in vivo by pravastatin  [Not invited]
    The 73rd Annual Scientific Meeting of the Japanese Circulation Society  2009
  • Crucial role of unprocessed RhoA and Rac1 in progression of atherosclerosis in Watanabe heritable hyperlipidemic (WHHL) Rabbits  [Not invited]
    第41回 日本動脈硬化学会学術総会  2009
  • Integral role of receptor for advanced glycation end products (RAGE) in non-diabetic atherosclerosis  [Not invited]
    第41回 日本動脈硬化学会学術総会  2009
  • Membrane type matrix metalloproteinase-1 (MT1-MMP) is a cell surface modifier of RhoA/Rac1-mediated signaling pathways and forms a complex with LOX-1 in oxidized LDL-induced endothelial dysfunction  [Not invited]
    Congress of the European Society of Cardiology 2009  2009
  • Cooperation of MT1-MMP and LOX-1 for RhoA- and Rac1-dependent signaling pathways in oxidized LDL-mediated endothelial dysfunction and atherosclerosis  [Not invited]
    Scientific Sessions of American Heart Association 2009  2009
  • 非糖尿病性動脈硬化病変におけるRAGE (Receptor for advanced glycation end products) の役割  [Not invited]
    第37回 日本心脈管作動物質学会  2008
  • 上北洋徳*, 石橋敏幸*, 杉本浩一*, 坂本信雄*, 大河原浩*, 上岡正志*, 竹石恭知*, 塩見雅志, 山岸昌一, 丸山幸夫  [Not invited]
    第11回 福島動脈硬化Forum  2008
  • 非糖尿病性動脈硬化病変におけるRAGE (Receptor for advanced glycation end products) の役割  [Not invited]
    第40回 日本動脈硬化学会総会・学術集会  2008
  • 血管内皮細胞機能不全と活性酸素種産生に対するプラバスタチン静脈内投与の効果とその機序  [Not invited]
    第40回 日本動脈硬化学会総会・学術集会  2008
  • 非糖尿病性動脈硬化病変におけるRAGE (Receptor for advanced glycation end products) の役割(ワークショップ)  [Not invited]
    第3回 ウサギフォーラム ー医療に貢献する実験用ウサギの新しい展開-  2008
  • New insights into oxidized LDL-induced RhoA and Rac1 activation by an LOX-1-MT1-MMP axis in endothelial dysfunction  [Not invited]
    第72回 日本循環器学会学術集会  2008
  • Atorvastatin blocks an advanced glycation end products (AGE)/ receptor for AGE (RAGE) axis in diabetic vascular responses  [Not invited]
    第72回 日本循環器学会学術集会  2008
  • Therapeutic targeting RAGE/MT-1-MMP/Rac1 axis in redox-sensitive signaling pathway in diabetic vascular remodeling  [Not invited]
    Scientific Sessions of American Heart Association 2008  2008
  • Receptor for advanced glycation end products (RAGE) plays an integral role in non-diabetic atherosclerosis and oxidized LDL stimulation  [Not invited]
    Scientific Sessions of American Heart Association 2008  2008
  • Therapeutic targeting RAGE/MT-1-MMP/Rac1 axis in redox-sensitive signaling pathway in diabetic vascular remodeling  [Not invited]
    Scientific Sessions of American Heart Association 2008  2008
  • Receptor for advanced glycation end products (RAGE) plays an integral role in non-diabetic atherosclerosis and oxidized LDL stimulation  [Not invited]
    Scientific Sessions of American Heart Association 2008  2008
  • 内皮細胞(EC)への単球接着系でのレドックスおよびカルシウムシグナルにおける酸化LDL受容体LOX-1の役割  [Not invited]
    第143回 日本循環器学会東北地方会  2007
  • Receptor for advanced glycation end products (RAGE) plays a crucial role in atherosclerosis  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • Pravastatin rapidly prevents thrombin-induced vasoconstriction and NADPH oxidase-dependent ROS generation in vivo: implications for beneficial effect  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • LOX-1 plays a crucial role in RhoA- and Rac1-dependent signaling through Gi induced by oxidized low-density lipoprotein in endothelial cells  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • New insights into regulation of diabetic vascular remodeling by an MT1-MMP-RAGE axis  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • RhoA-dependent PAI-1 gene expression induced in endothelial cells by monocyte adhesion is mediated via geranylgeranyl transferase I and Ca2+ signaling  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • バルサルバ洞動脈瘤及び右室穿通を来した感染性心内膜炎の一例  [Not invited]
    第18回 日本心エコー図学会学術集会  2007
  • 動脈硬化病変の進展におけるRAGE (Receptor for Advanced Glycation End Products) の役割  [Not invited]
    第39回 日本動脈硬化学会総会・学術集会  2007
  • 糖尿病性血管病変における分子機構の解明-AGE/RAGE/MT1-MMP系とレニン・アンジオテンシン系のcross talkの観点から-  [Not invited]
    第39回 日本動脈硬化学会総会・学術集会  2007
  • Receptor for advanced glycation end products (RAGE) plays a crucial role in atherosclerosis  [Not invited]
    第71回 日本循環器学会学術集会  2007
  • Pravastatin rapidly prevents thrombin-induced vasoconstriction and NADPH oxidase-dependent ROS generation in vivo: implications for beneficial effect  [Not invited]
    2007
  • LOX-1 plays a crucial role in RhoA- and Rac1-dependent signaling through Gi induced by oxidized low-density lipoprotein in endothelial cells  [Not invited]
    2007
  • New insights into regulation of diabetic vascular remodeling by an MT1-MMP-RAGE axis  [Not invited]
    2007
  • RhoA-dependent PAI-1 gene expression induced in endothelial cells by monocyte adhesion is mediated via geranylgeranyl transferase I and Ca2+ signaling  [Not invited]
    2007

Works

  • 自律神経叢修飾による新しい心房細動治療戦略の開発
    2012/04 -2015/03

MISC

  • Masashi Kamioka, Hitoshi Suzuki, Yoshiyuki Matsumoto, Minoru Nodera, Takashi Kaneshiro, Yoshiyuki Kamiyama, Yasuchika Takeishi  CIRCULATION  134-  2016/11  [Not refereed][Not invited]
  • Masashi Kamioka, Hitoshi Suzuki, Yoshiyuki Matsumoto, Minoru Nodera, Takashi Kaneshiro, Yoshiyuki Kamiyama, Yasuchika Takeishi  CIRCULATION  134-  2016/11  [Not refereed][Not invited]
  • Minoru Nodera, Hitoshi Suzuki, Shinya Yamada, Masashi Kamioka, Takashi Kaneshiro, Yoshiyuki Kamiyama, Yasuchika Takeishi  INTERNATIONAL HEART JOURNAL  56-  (6)  613  -617  2015/11  [Not refereed][Not invited]
     
    Several studies have demonstrated that oral intake of n-3 polyunsaturated fatty acids, specifically eicosapentaenoic acid (EPA), prevents ventricular tachyarrhythmias (VT) with ischemic heart disease, but the underlying mechanisms still remain unclear. Thus, we examined the relation between the serum EPA/arachidonic acid (AA) ratio and electrophysiological properties in patients with ischemic heart disease. The study subjects consisted of 57 patients (46 males, mean age, 66 +/- 13 years) with ischemic heart disease. T-wave alternans (TWA) and heart rate variability were assessed by 24-hour Holier ECG, and left ventricular ejection fraction (LVEF) was determined by echocardiography. Fasting blood samples were collected, and the serum EPA/AA ratio was determined. Based on a median value of the serum EPA/AA ratio, all subjects were divided into two groups: serum EPA/AA ratio below 0.33 (Group-L, n = 28) or not (Group-H, n = 29). We compared these parameters between the two groups. LVEF was not different between the two groups. The maximum value of TWA was significantly higher in Group-L than in Group-H (69.5 +/- 22.8 mu V versus 48.7 +/- 12.0 mu V, P = 0.007). In addition, VT defined as above 3 beats was observed in 7 cases (25%) in Group-L, but there were no cases of VT in Group-H (P = 0.004). However, low-frequency (LF) component, high-frequency (HF) component, LF to BF ratio, and standard deviation of all R-R intervals were not different between the two groups. These results suggest that a low EPA/AA ratio may induce cardiac electrical instability, but not autonomic nervous imbalance, associated with VT in patients with ischemic heart disease.
  • Minoru Nodera, Hitoshi Suzuki, Shinya Yamada, Masashi Kamioka, Takashi Kaneshiro, Yoshiyuki Kamiyama, Yasuchika Takeishi  INTERNATIONAL HEART JOURNAL  56-  (6)  613  -617  2015/11  [Not refereed][Not invited]
     
    Several studies have demonstrated that oral intake of n-3 polyunsaturated fatty acids, specifically eicosapentaenoic acid (EPA), prevents ventricular tachyarrhythmias (VT) with ischemic heart disease, but the underlying mechanisms still remain unclear. Thus, we examined the relation between the serum EPA/arachidonic acid (AA) ratio and electrophysiological properties in patients with ischemic heart disease. The study subjects consisted of 57 patients (46 males, mean age, 66 +/- 13 years) with ischemic heart disease. T-wave alternans (TWA) and heart rate variability were assessed by 24-hour Holier ECG, and left ventricular ejection fraction (LVEF) was determined by echocardiography. Fasting blood samples were collected, and the serum EPA/AA ratio was determined. Based on a median value of the serum EPA/AA ratio, all subjects were divided into two groups: serum EPA/AA ratio below 0.33 (Group-L, n = 28) or not (Group-H, n = 29). We compared these parameters between the two groups. LVEF was not different between the two groups. The maximum value of TWA was significantly higher in Group-L than in Group-H (69.5 +/- 22.8 mu V versus 48.7 +/- 12.0 mu V, P = 0.007). In addition, VT defined as above 3 beats was observed in 7 cases (25%) in Group-L, but there were no cases of VT in Group-H (P = 0.004). However, low-frequency (LF) component, high-frequency (HF) component, LF to BF ratio, and standard deviation of all R-R intervals were not different between the two groups. These results suggest that a low EPA/AA ratio may induce cardiac electrical instability, but not autonomic nervous imbalance, associated with VT in patients with ischemic heart disease.
  • Masashi Kamioka, Shibu Mathew, Tina Lin, Andreas Metzner, Andreas Rillig, Sebastian Deiss, Peter Rausch, Christine Lemes, Hisaki Makimoto, Hesheng Hu, Dongpo Liang, Erik Wissner, Roland Richard Tilz, Karl-Heinz Kuck, Feifan Ouyang  CLINICAL RESEARCH IN CARDIOLOGY  104-  (7)  544  -554  2015/07  [Not refereed][Not invited]
     
    Ventricular arrhythmias (VAs) from the left ventricular outflow tract (LVOT) can originate from within or below the aortic sinus of valsalva (ASV). Mapping and ablation below the ASV is challenging and there are limited data predicting VA origins using electrocardiographic and electrophysiological features. Thirty-four patients (56.7 +/- A 15.2 years; 19 males) with symptomatic VAs were analyzed. VA origins were determined by successful ablation. Patients were classified into 2 groups (group 1, VAs within the ASV; group 2, VAs below the ASV). Local activation and QRS morphology were compared between these 2 groups. Twelve patients were classified as group 1 and 22 as group 2. Presystolic potentials (PPs) during VAs were present in 11 patients (91 %) in group 1 and 3 (13 %) in group 2. S-wave amplitude and duration in lead I were lower and shorter in group 1 vs. group 2, respectively. Q-wave aV(L)/aV(R) ratio (Q-aV(L)/aV(R)) was smaller in group 1 vs. group 2. No group 1 patients had Q-aV(L)/aV(R) > 1.45. PPs in the ASV was the strongest independent predictor for VAs originating within the ASV (OR: 30.003, P = 0.006). Deeper and longer S-waves in lead I and Q-aV(L)/aV(R) > 1.45 suggest VAs originating below the ASV. Local PPs strongly suggest an origin within the ASV. ECG characteristics combined with local PPs can be a practical guide for ablating LVOT-VAs.
  • Masashi Kamioka, Shibu Mathew, Tina Lin, Andreas Metzner, Andreas Rillig, Sebastian Deiss, Peter Rausch, Christine Lemes, Hisaki Makimoto, Hesheng Hu, Dongpo Liang, Erik Wissner, Roland Richard Tilz, Karl-Heinz Kuck, Feifan Ouyang  CLINICAL RESEARCH IN CARDIOLOGY  104-  (7)  544  -554  2015/07  [Not refereed][Not invited]
     
    Ventricular arrhythmias (VAs) from the left ventricular outflow tract (LVOT) can originate from within or below the aortic sinus of valsalva (ASV). Mapping and ablation below the ASV is challenging and there are limited data predicting VA origins using electrocardiographic and electrophysiological features. Thirty-four patients (56.7 +/- A 15.2 years; 19 males) with symptomatic VAs were analyzed. VA origins were determined by successful ablation. Patients were classified into 2 groups (group 1, VAs within the ASV; group 2, VAs below the ASV). Local activation and QRS morphology were compared between these 2 groups. Twelve patients were classified as group 1 and 22 as group 2. Presystolic potentials (PPs) during VAs were present in 11 patients (91 %) in group 1 and 3 (13 %) in group 2. S-wave amplitude and duration in lead I were lower and shorter in group 1 vs. group 2, respectively. Q-wave aV(L)/aV(R) ratio (Q-aV(L)/aV(R)) was smaller in group 1 vs. group 2. No group 1 patients had Q-aV(L)/aV(R) > 1.45. PPs in the ASV was the strongest independent predictor for VAs originating within the ASV (OR: 30.003, P = 0.006). Deeper and longer S-waves in lead I and Q-aV(L)/aV(R) > 1.45 suggest VAs originating below the ASV. Local PPs strongly suggest an origin within the ASV. ECG characteristics combined with local PPs can be a practical guide for ablating LVOT-VAs.
  • Andreas Rillig, Tina Lin, Andre Burchard, Masashi Kamioka, Christian Heeger, Hisaki Makimoto, Andreas Metzner, Erik Wissner, Peter Wohlmuth, Feifan Ouyang, Karl-Heinz Kuck, Roland Richard Tilz  EUROPACE  17-  (3)  396  -402  2015/03  [Not refereed][Not invited]
     
    Aims The multipolar irrigated radiofrequency (RF) ablation catheter (nMARQ (TM)) is a novel tool for pulmonary vein isolation (PVI). We investigated the incidence of thermal oesophageal injury (El) using the nMARQ (TM) for PVI. Methods and results In the initial six patients (Group 1), RF was delivered at the posterior wall with a maximum duration of 60 sand a maximum power (maxP) of 20W for unipolar ablation, and a maxP of 10W for the bipolar ablation. In the Latter 15 patients (Group 2), RF application was Limited at the posterior wall to a maximum duration of 30 sand a maxP of 15 Wfor unipolar ablation a max P of 10W for bipolar ablation. Oesophageal temperature monitoring was performed in all patients and ablation was terminated at a temperature rise >41 degrees C. Endoscopy was carried out within 2 days post-ablation. Pulmonary vein isolation was performed during sinus rhythm and was successfully achieved in 83 of 84 PVs except the septal inferior vein in one patient. Charring was seen in 3 of 21 (14.3%) patients without any evidence of embolism. Phrenic nerve patsy occurred in one patient. Endoscopy revealed severe El in 3 of 6 (50%) patients in Group 1 and in 1 of 15 patients (6.7%) in Group 2. Procedure times between Groups 1 and 2 were similar (228.3 + 60.2 min vs. 221.3 + 51.8 min; P = 0.79). Conclusion An unexpectedly high incidence of thermal El was noted following PVI using the nMARQ (TM) with the initial ablation protocol. However, the incidence of thermal El can be sigificantly reduced with limited power and RF application time at the posterior Left atrium.
  • Andreas Rillig, Tina Lin, Andre Burchard, Masashi Kamioka, Christian Heeger, Hisaki Makimoto, Andreas Metzner, Erik Wissner, Peter Wohlmuth, Feifan Ouyang, Karl-Heinz Kuck, Roland Richard Tilz  EUROPACE  17-  (3)  396  -402  2015/03  [Not refereed][Not invited]
     
    Aims The multipolar irrigated radiofrequency (RF) ablation catheter (nMARQ (TM)) is a novel tool for pulmonary vein isolation (PVI). We investigated the incidence of thermal oesophageal injury (El) using the nMARQ (TM) for PVI. Methods and results In the initial six patients (Group 1), RF was delivered at the posterior wall with a maximum duration of 60 sand a maximum power (maxP) of 20W for unipolar ablation, and a maxP of 10W for the bipolar ablation. In the Latter 15 patients (Group 2), RF application was Limited at the posterior wall to a maximum duration of 30 sand a maxP of 15 Wfor unipolar ablation a max P of 10W for bipolar ablation. Oesophageal temperature monitoring was performed in all patients and ablation was terminated at a temperature rise >41 degrees C. Endoscopy was carried out within 2 days post-ablation. Pulmonary vein isolation was performed during sinus rhythm and was successfully achieved in 83 of 84 PVs except the septal inferior vein in one patient. Charring was seen in 3 of 21 (14.3%) patients without any evidence of embolism. Phrenic nerve patsy occurred in one patient. Endoscopy revealed severe El in 3 of 6 (50%) patients in Group 1 and in 1 of 15 patients (6.7%) in Group 2. Procedure times between Groups 1 and 2 were similar (228.3 + 60.2 min vs. 221.3 + 51.8 min; P = 0.79). Conclusion An unexpectedly high incidence of thermal El was noted following PVI using the nMARQ (TM) with the initial ablation protocol. However, the incidence of thermal El can be sigificantly reduced with limited power and RF application time at the posterior Left atrium.
  • Hiroyuki Kunii, Tetsuro Yokokawa, Akihiko Sato, Masashi Kamioka, Akiomi Yoshihisa, Takayoshi Yamaki, Gaku Nagazawa, Kazuhiko Nakazato, Yasuchika Takeishi  Journal of Cardiology Cases  10-  (5)  171  -175  2014/11  [Not refereed][Not invited]
     
    We report a case with acute coronary syndrome due to in-stent plaque rupture that occurred 9 years after bare metal stent deployment. From the results of intravascular imaging and pathological findings, we diagnosed this case as acute coronary syndrome caused by in-stent plaque rupture. In-stent neoatherosclerosis is an important mechanism of very late stent thrombosis, and the intravascular imaging is helpful to identify this and to decide clinical judgment.< . Learning objective: In-stent neoatherosclerosis is an important mechanism of very late stent thrombosis, and the intravascular imaging is helpful to identify this and to decide clinical judgment> .
  • Hiroyuki Kunii, Tetsuro Yokokawa, Akihiko Sato, Masashi Kamioka, Akiomi Yoshihisa, Takayoshi Yamaki, Gaku Nagazawa, Kazuhiko Nakazato, Yasuchika Takeishi  Journal of Cardiology Cases  10-  (5)  171  -175  2014/11  [Not refereed][Not invited]
     
    We report a case with acute coronary syndrome due to in-stent plaque rupture that occurred 9 years after bare metal stent deployment. From the results of intravascular imaging and pathological findings, we diagnosed this case as acute coronary syndrome caused by in-stent plaque rupture. In-stent neoatherosclerosis is an important mechanism of very late stent thrombosis, and the intravascular imaging is helpful to identify this and to decide clinical judgment.< . Learning objective: In-stent neoatherosclerosis is an important mechanism of very late stent thrombosis, and the intravascular imaging is helpful to identify this and to decide clinical judgment> .
  • Roland Richard Tilz, Hisaki Makimoto, Tina Lin, Andreas Rillig, Andreas Metzner, Shibu Mathew, Sebastian Deiss, Erik Wissner, Peter Rausch, Masashi Kamioka, Christian Heeger, Karl-Heinz Kuck, Feifan Ouyang  EUROPACE  16-  (9)  1387  -1395  2014/09  [Not refereed][Not invited]
     
    Aims Clinical outcomes following radiofrequency ablation of ventricular tachycardias (VTs) depend on catheter tip-to-tissue contact force (CF). Left-ventricular (LV) mapping is performed via antegrade-transseptal or retrograde-transaortic approaches, and the applied CF may depend on the approach used. This study evaluated (i) the impact of antegrade-transseptal vs. retrograde- transaortic LV-mapping approaches on CF and catheter stability and (ii) the clinical value of the commonly used surrogate markers of catheter-myocardial contact-impedance, unipolar, and bipolar electrogram amplitudes. Methods and results An antegrade-transseptal and a retrograde-transaortic LV-mapping approach was performed in 10 patients undergoing VT ablation by using CF-sensing catheters. Operators were blinded to CF data and data were analysed according to 11 predefined LV segments. Three thousand three hundred and twenty-four mapping points (1577 antegrade, 1747 retrograde) were analysed, including 80 (2.4%) points with maximum CF > 100 g. Median antegrade and retrograde CF were 16.0 g (q1-q3; 8.4-26.2) and 15.3 g (9.8-23.4), respectively. Contact force was significantly higher antegradely in mid-anteroseptum, mid-lateral, and apical segments, and significantly higher retrogradely in basal-anteroseptum, basal-inferoseptum, basal-inferior, and basal-lateral segments. Contact force did correlate with impedance, unipolar, and bipolar electrogram amplitudes; however, there were large overlaps. Conclusions Antegrade vs. retrograde LV-mapping approaches result in different CF. A combined approach to the LV mapping may improve the overall LV mapping, potentially resulting in better clinical outcomes for the left VT catheter ablation. The previous surrogate markers used to assess CF docorrelate with in vivo CF; however, due to a larger over lap, their clinical value is limited.
  • Roland Richard Tilz, Hisaki Makimoto, Tina Lin, Andreas Rillig, Andreas Metzner, Shibu Mathew, Sebastian Deiss, Erik Wissner, Peter Rausch, Masashi Kamioka, Christian Heeger, Karl-Heinz Kuck, Feifan Ouyang  EUROPACE  16-  (9)  1387  -1395  2014/09  [Not refereed][Not invited]
     
    Aims Clinical outcomes following radiofrequency ablation of ventricular tachycardias (VTs) depend on catheter tip-to-tissue contact force (CF). Left-ventricular (LV) mapping is performed via antegrade-transseptal or retrograde-transaortic approaches, and the applied CF may depend on the approach used. This study evaluated (i) the impact of antegrade-transseptal vs. retrograde- transaortic LV-mapping approaches on CF and catheter stability and (ii) the clinical value of the commonly used surrogate markers of catheter-myocardial contact-impedance, unipolar, and bipolar electrogram amplitudes. Methods and results An antegrade-transseptal and a retrograde-transaortic LV-mapping approach was performed in 10 patients undergoing VT ablation by using CF-sensing catheters. Operators were blinded to CF data and data were analysed according to 11 predefined LV segments. Three thousand three hundred and twenty-four mapping points (1577 antegrade, 1747 retrograde) were analysed, including 80 (2.4%) points with maximum CF > 100 g. Median antegrade and retrograde CF were 16.0 g (q1-q3; 8.4-26.2) and 15.3 g (9.8-23.4), respectively. Contact force was significantly higher antegradely in mid-anteroseptum, mid-lateral, and apical segments, and significantly higher retrogradely in basal-anteroseptum, basal-inferoseptum, basal-inferior, and basal-lateral segments. Contact force did correlate with impedance, unipolar, and bipolar electrogram amplitudes; however, there were large overlaps. Conclusions Antegrade vs. retrograde LV-mapping approaches result in different CF. A combined approach to the LV mapping may improve the overall LV mapping, potentially resulting in better clinical outcomes for the left VT catheter ablation. The previous surrogate markers used to assess CF docorrelate with in vivo CF; however, due to a larger over lap, their clinical value is limited.
  • Feifan Ouyang, Shibu Mathew, Shulin Wu, Masashi Kamioka, Andreas Metzner, Yumei Xue, Weizhu Ju, Bing Yang, Xianzhang Zhan, Andreas Rillig, Tina Lin, Peter Rausch, Sebastian Deiss, Christine Lemes, Tobias Toennis, Erik Wissner, Roland Richard Tilz, Karl-Heinz Kuck, Minglong Chen  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (3)  445  -455  2014/06  [Not refereed][Not invited]
     
    Background Ventricular arrhythmias (VAs) originating from the anterosuperior left ventricular outflow tract (LVOT) represent a challenging location for catheter ablation. This study investigates mapping and ablation of VA from anterosuperior LVOT via a transseptal approach. Methods and Results This study included 27 patients with symptomatic VA, of which 13 patients had previous failed ablations. LVOT endocardial 3-dimensional mapping via retrograde transaortic and antegrade transseptal approaches was performed. Previous ECG markers for procedure failure were analyzed. In all patients, earliest activation with low-amplitude potentials was identified at the anterosuperior LVOT 5.12.8 mm below the aortic cusp and preceded the QRS onset by 39.5 +/- 7.7 ms only via an antegrade transseptal approach using a reversed S curve. In all patients, pace mapping failed to demonstrate perfect QRS morphology match. The anatomic location was below the left coronary cusp in 16, below the left coronary cusp/right coronary cusp junction in 8, and below the right coronary cusp in 3 patients. Radiofrequency energy resulted in rapid disappearance of VAs in all patients. ECG analysis showed aVL/aVR Q-wave amplitude ratio >1.4 in 7, lead III/II R-wave amplitude ratio >1.1 in 10, and peak deflection index >0.6 in 11 patients. There were no complications or clinical VA recurrence during a mean follow-up of 8.4 +/- 2.5 months. Conclusions The anterosuperior LVOT can be reached via a transseptal approach with a reversed S curve of the ablation catheter. The rapid effect from radiofrequency energy indicates that the VA is most likely located under the endocardium. Also, previous ECG markers for procedure failure need further investigation.
  • Feifan Ouyang, Shibu Mathew, Shulin Wu, Masashi Kamioka, Andreas Metzner, Yumei Xue, Weizhu Ju, Bing Yang, Xianzhang Zhan, Andreas Rillig, Tina Lin, Peter Rausch, Sebastian Deiss, Christine Lemes, Tobias Toennis, Erik Wissner, Roland Richard Tilz, Karl-Heinz Kuck, Minglong Chen  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (3)  445  -455  2014/06  [Not refereed][Not invited]
     
    Background Ventricular arrhythmias (VAs) originating from the anterosuperior left ventricular outflow tract (LVOT) represent a challenging location for catheter ablation. This study investigates mapping and ablation of VA from anterosuperior LVOT via a transseptal approach. Methods and Results This study included 27 patients with symptomatic VA, of which 13 patients had previous failed ablations. LVOT endocardial 3-dimensional mapping via retrograde transaortic and antegrade transseptal approaches was performed. Previous ECG markers for procedure failure were analyzed. In all patients, earliest activation with low-amplitude potentials was identified at the anterosuperior LVOT 5.12.8 mm below the aortic cusp and preceded the QRS onset by 39.5 +/- 7.7 ms only via an antegrade transseptal approach using a reversed S curve. In all patients, pace mapping failed to demonstrate perfect QRS morphology match. The anatomic location was below the left coronary cusp in 16, below the left coronary cusp/right coronary cusp junction in 8, and below the right coronary cusp in 3 patients. Radiofrequency energy resulted in rapid disappearance of VAs in all patients. ECG analysis showed aVL/aVR Q-wave amplitude ratio >1.4 in 7, lead III/II R-wave amplitude ratio >1.1 in 10, and peak deflection index >0.6 in 11 patients. There were no complications or clinical VA recurrence during a mean follow-up of 8.4 +/- 2.5 months. Conclusions The anterosuperior LVOT can be reached via a transseptal approach with a reversed S curve of the ablation catheter. The rapid effect from radiofrequency energy indicates that the VA is most likely located under the endocardium. Also, previous ECG markers for procedure failure need further investigation.
  • Hisaki Makimoto, Roland Richard Tilz, Tina Lin, Andreas Rillig, Shibu Mathew, Sebastian Deiss, Erik Wissner, Andreas Metzner, Peter Rausch, Alexander Bardyszewski, Qingyong Zhang, Masashi Kamioka, Christine Lemes, Karl-Heinz Kucx, Feifan Ouyang  INTERNATIONAL HEART JOURNAL  55-  (3)  249  -255  2014/05  [Not refereed][Not invited]
     
    In addition to contact force (CF), catheter stability is considered to be an important factor in creating radiofrequency lesions. To evaluate the catheter stability during pulmonary vein isolation (PVI) using CF-sensing catheter. PVI was performed in 32 patients using a CF-sensing catheter. Operators were blinded to CF. The application was arbitrarily defined as a "visually unstable" point if the catheter moved >= 4 mm Data were analyzed according to 6 predefined segments for the ipsilateral PVs. As a parameter of catheter stability, the standard deviation (SD) of CF and relative standard deviation (RSD = 100 x SD of CF/average CF) were introduced. A total of 932 RF applications with 426 visually unstable points (UP; 45.7%) and 506 stable points (SP; 54.3%) were analyzed. SD was significantly higher at UP (8.0g versus 5.7g, P < 0.001), and RSD was significantly higher at UP (43.7% versus 26.5%, P < 0.001). Higher RSD was associated with visual instability in all the segments of both PVs, however, higher SD of CF was not in all segments. At the antero-superior segment of the LPV, and the roof and postero-inferior segments of the RPV, the RSD values were over 50%, suggesting catheter instability. Catheter instability occurred in 45% of ablations during PVI and was predominantly located in the antero-superior segment of the LPV and postero-inferior segment of the RPV, which may result in incomplete lesion formation. RSD had significant correlation with visual catheter stability.
  • Andreas Metzner, Peter Rausch, Christine Lemes, Bruno Reissmann, Alexander Bardyszewski, Roland Tilz, Andreas Rillig, Shibu Mathew, Sebastian Deiss, Masashi Kamioka, Tobias Toennis, Tina Lin, Feifan Ouyang, Karl-Heinz Kuck, Erik Wissner  JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY  25-  (5)  466  -470  2014/05  [Not refereed][Not invited]
     
    The Incidence of Phrenic Nerve Injury Introduction The second-generation cryoballoon (CB; Arctic Front Advance, Medtronic Inc., Minneapolis, MN, USA) has demonstrated greater procedural efficacy compared to the original CB. Whether increased efficacy translates into a higher incidence of phrenic nerve (PN) injury needs further evaluation. Materials and Methods In patients with drug-refractory paroxysmal atrial fibrillation (AF) or short-standing persistent AF, pulmonary vein isolation (PVI) was performed using the 28 mm second-generation CB. During cryoenergy delivery along the septal PVs, continuous PN pacing was performed. The freeze cycle was aborted in case of weakening or loss of diaphragmatic contraction. Results A total of 115 patients (42 female, mean age 61 +/- 11 years, mean LA-diameter 43 +/- 6 mm) with a history of paroxysmal AF (93/115 patients [81%]) or short-standing persistent AF (22/115 patients [19%]) underwent CB-based PVI. A total 445 of 448 (99%) PVs were isolated successfully. PN palsy (PNP) occurred in 4 of 115 (3.5%) patients, while applying cryoenergy to the right superior PV. Despite prompt interruption of the freezing cycle, PN function failed to recover during the periprocedural phase. PN recovery was observed as late as 10 months postablation. Conclusions Using the second-generation 28 mm CB, PNP occurred in 4 of 115 (3.5%) patients. While 1 of 4 PNP recovered 10 months after ablation, long-term outcome in the remaining 3 patients is currently unknown due to the rather short follow-up period.
  • Hisaki Makimoto, Roland Richard Tilz, Tina Lin, Andreas Rillig, Shibu Mathew, Sebastian Deiss, Erik Wissner, Andreas Metzner, Peter Rausch, Alexander Bardyszewski, Qingyong Zhang, Masashi Kamioka, Christine Lemes, Karl-Heinz Kucx, Feifan Ouyang  INTERNATIONAL HEART JOURNAL  55-  (3)  249  -255  2014/05  [Not refereed][Not invited]
     
    In addition to contact force (CF), catheter stability is considered to be an important factor in creating radiofrequency lesions. To evaluate the catheter stability during pulmonary vein isolation (PVI) using CF-sensing catheter. PVI was performed in 32 patients using a CF-sensing catheter. Operators were blinded to CF. The application was arbitrarily defined as a "visually unstable" point if the catheter moved >= 4 mm Data were analyzed according to 6 predefined segments for the ipsilateral PVs. As a parameter of catheter stability, the standard deviation (SD) of CF and relative standard deviation (RSD = 100 x SD of CF/average CF) were introduced. A total of 932 RF applications with 426 visually unstable points (UP; 45.7%) and 506 stable points (SP; 54.3%) were analyzed. SD was significantly higher at UP (8.0g versus 5.7g, P < 0.001), and RSD was significantly higher at UP (43.7% versus 26.5%, P < 0.001). Higher RSD was associated with visual instability in all the segments of both PVs, however, higher SD of CF was not in all segments. At the antero-superior segment of the LPV, and the roof and postero-inferior segments of the RPV, the RSD values were over 50%, suggesting catheter instability. Catheter instability occurred in 45% of ablations during PVI and was predominantly located in the antero-superior segment of the LPV and postero-inferior segment of the RPV, which may result in incomplete lesion formation. RSD had significant correlation with visual catheter stability.
  • Andreas Metzner, Peter Rausch, Christine Lemes, Bruno Reissmann, Alexander Bardyszewski, Roland Tilz, Andreas Rillig, Shibu Mathew, Sebastian Deiss, Masashi Kamioka, Tobias Toennis, Tina Lin, Feifan Ouyang, Karl-Heinz Kuck, Erik Wissner  JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY  25-  (5)  466  -470  2014/05  [Not refereed][Not invited]
     
    The Incidence of Phrenic Nerve Injury Introduction The second-generation cryoballoon (CB; Arctic Front Advance, Medtronic Inc., Minneapolis, MN, USA) has demonstrated greater procedural efficacy compared to the original CB. Whether increased efficacy translates into a higher incidence of phrenic nerve (PN) injury needs further evaluation. Materials and Methods In patients with drug-refractory paroxysmal atrial fibrillation (AF) or short-standing persistent AF, pulmonary vein isolation (PVI) was performed using the 28 mm second-generation CB. During cryoenergy delivery along the septal PVs, continuous PN pacing was performed. The freeze cycle was aborted in case of weakening or loss of diaphragmatic contraction. Results A total of 115 patients (42 female, mean age 61 +/- 11 years, mean LA-diameter 43 +/- 6 mm) with a history of paroxysmal AF (93/115 patients [81%]) or short-standing persistent AF (22/115 patients [19%]) underwent CB-based PVI. A total 445 of 448 (99%) PVs were isolated successfully. PN palsy (PNP) occurred in 4 of 115 (3.5%) patients, while applying cryoenergy to the right superior PV. Despite prompt interruption of the freezing cycle, PN function failed to recover during the periprocedural phase. PN recovery was observed as late as 10 months postablation. Conclusions Using the second-generation 28 mm CB, PNP occurred in 4 of 115 (3.5%) patients. While 1 of 4 PNP recovered 10 months after ablation, long-term outcome in the remaining 3 patients is currently unknown due to the rather short follow-up period.
  • Andreas Metzner, Bruno Reissmann, Peter Rausch, Shibu Mathew, Peter Wohlmuth, Roland Tilz, Andreas Rillig, Christine Lemes, Sebastian Deiss, Christian Heeger, Masashi Kamioka, Tina Lin, Feifan Ouyang, Karl-Heinz Kuck, Erik Wissner  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (2)  288  -292  2014/04  [Not refereed][Not invited]
     
    Background The use of second-generation cryoballoon for pulmonary vein isolation in patients with paroxysmal atrial fibrillation has demonstrated encouraging acute and mid-term results. Long-term outcome data are not yet available. Methods and Results Fifty patients (18 women; mean age, 6111 years; mean left atrial diameter, 43 +/- 5 mm) with paroxysmal (36 of 50 patients; 72%) or short-standing (<3-month duration) persistent atrial fibrillation (14 of 50 patients; 28%) underwent cryoballoon-based pulmonary vein isolation. Freeze cycle duration was 240 seconds. After successful pulmonary vein isolation, a bonus freeze was applied. Follow-up was based on outpatient clinic visits at 3, 6, and 12 months including Holter-ECGs and telephonic interviews. Recurrence was defined as a symptomatic or documented arrhythmia episode >30 seconds excluding a 3-month blanking period. A total of 192 pulmonary veins were identified, and 191 of 192 (99%) pulmonary veins were successfully isolated. Phrenic nerve palsy occurred in 1 of 50 (2%) patients. Follow-up was available for 49 of 50 (98%) patients with a mean follow-up duration of 440 +/- 39 days. Thirty-nine of 49 (80%) patients remained in stable sinus rhythm. Of 8 of 10 patients with arrhythmia recurrence, a second procedure using radiofrequency ablation demonstrated left atrial to pulmonary vein reconduction. Conclusions The use of second-generation 28-mm cryoballoon for pulmonary vein isolation results in an 80% 1-year success rate.
  • Andreas Metzner, Bruno Reissmann, Peter Rausch, Shibu Mathew, Peter Wohlmuth, Roland Tilz, Andreas Rillig, Christine Lemes, Sebastian Deiss, Christian Heeger, Masashi Kamioka, Tina Lin, Feifan Ouyang, Karl-Heinz Kuck, Erik Wissner  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (2)  288  -292  2014/04  [Not refereed][Not invited]
     
    Background The use of second-generation cryoballoon for pulmonary vein isolation in patients with paroxysmal atrial fibrillation has demonstrated encouraging acute and mid-term results. Long-term outcome data are not yet available. Methods and Results Fifty patients (18 women; mean age, 6111 years; mean left atrial diameter, 43 +/- 5 mm) with paroxysmal (36 of 50 patients; 72%) or short-standing (<3-month duration) persistent atrial fibrillation (14 of 50 patients; 28%) underwent cryoballoon-based pulmonary vein isolation. Freeze cycle duration was 240 seconds. After successful pulmonary vein isolation, a bonus freeze was applied. Follow-up was based on outpatient clinic visits at 3, 6, and 12 months including Holter-ECGs and telephonic interviews. Recurrence was defined as a symptomatic or documented arrhythmia episode >30 seconds excluding a 3-month blanking period. A total of 192 pulmonary veins were identified, and 191 of 192 (99%) pulmonary veins were successfully isolated. Phrenic nerve palsy occurred in 1 of 50 (2%) patients. Follow-up was available for 49 of 50 (98%) patients with a mean follow-up duration of 440 +/- 39 days. Thirty-nine of 49 (80%) patients remained in stable sinus rhythm. Of 8 of 10 patients with arrhythmia recurrence, a second procedure using radiofrequency ablation demonstrated left atrial to pulmonary vein reconduction. Conclusions The use of second-generation 28-mm cryoballoon for pulmonary vein isolation results in an 80% 1-year success rate.
  • Xuping Li, Erik Wissner, Masashi Kamioka, Hisaki Makimoto, Peter Rausch, Andreas Metzner, Shibu Mathew, Andreas Rillig, Roland Richard Tilz, Alexander Furnkranz, Qi Chen, Qingyong Zhang, Qiming Liu, Shenghua Zhou, Karl-Heinz Kuck, Feifan Ouyang  HEART RHYTHM  11-  (2)  330  -335  2014/02  [Not refereed][Not invited]
  • Hisaki Makimoto, Tina Lin, Andreas Rillig, Andreas Metzner, Peter Wohlmuth, Anita Arya, Matthias Antz, Shibu Mathew, Sebastian Deiss, Erik Wissner, Peter Rausch, Aleksander Bardyszewski, Masashi Kamioka, Xuping Li, Karl-Heinz Kuck, Feifan Ouyang, Roland Richard Tilz  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (1)  46  -54  2014/02  [Not refereed][Not invited]
  • Xuping Li, Erik Wissner, Masashi Kamioka, Hisaki Makimoto, Peter Rausch, Andreas Metzner, Shibu Mathew, Andreas Rillig, Roland Richard Tilz, Alexander Furnkranz, Qi Chen, Qingyong Zhang, Qiming Liu, Shenghua Zhou, Karl-Heinz Kuck, Feifan Ouyang  HEART RHYTHM  11-  (2)  330  -335  2014/02  [Not refereed][Not invited]
  • Hisaki Makimoto, Tina Lin, Andreas Rillig, Andreas Metzner, Peter Wohlmuth, Anita Arya, Matthias Antz, Shibu Mathew, Sebastian Deiss, Erik Wissner, Peter Rausch, Aleksander Bardyszewski, Masashi Kamioka, Xuping Li, Karl-Heinz Kuck, Feifan Ouyang, Roland Richard Tilz  CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY  7-  (1)  46  -54  2014/02  [Not refereed][Not invited]
  • NAKAMURA, Yuichi, KUNII, Hiroyuki, YOSHIHISA, Akiomi, SATO, Akihiko, KAMIOKA, Masashi, NAKAZATO, Kazuhiko, SUZUKI, Hitoshi, SAITOH, Shu-ichi, HISA, Shinichi, TAKEISHI, Yasuchika  Journal of General Practice  2-  (4)  1000160  2014  [Not refereed][Not invited]
  • NAKAMURA, Yuichi, KUNII, Hiroyuki, YOSHIHISA, Akiomi, SATO, Akihiko, KAMIOKA, Masashi, NAKAZATO, Kazuhiko, SUZUKI, Hitoshi, SAITOH, Shu-ichi, HISA, Shinichi, TAKEISHI, Yasuchika  Journal of General Practice  2-  (4)  1000160  2014  [Not refereed][Not invited]
  • Shinya Yamada, Hitoshi Suzuki, Masashi Kamioka, Satoshi Suzuki, Yoshiyuki Kamiyama, Akiomi Yoshihisa, Shu-ichi Saitoh, Yasuchika Takeishi  CIRCULATION JOURNAL  77-  (6)  1466  -1473  2013/06  [Not refereed][Not invited]
     
    Background: It has been shown that sleep-disordered breathing (SDB) is associated with adverse prognosis in patients with chronic heart failure (CHF), but little is known about the relationship between SDB and life-threatening arrhythmias. Methods and Results: Fifty patients with CHF and SDB (33 male; mean age, 61 years) underwent Hotter electrocardiogram and portable sleep monitoring simultaneously. The circadian variation in positive T-wave alternans (TWA; >65 mu V) was determined during 6-h intervals (0-6, 6-12, 12-18, and 18-24h). In addition, power spectral analysis of heart rate variability (HRV) was evaluated across a 24-h period. The subjects were divided into 2 groups based on whether respiratory disturbance index was >= 20events/h (Group A, n=24) or not (Group B, n=26). The prevalence of positive TWA, parameters in HRV and the occurrence of ventricular tachycardia (>5 beats) were compared between the 2 groups. The prevalence of positive TWA in Group A was significantly higher than that in Group B in all 6-h intervals. Low-frequency and high-frequency powers of HRV were significantly lower in Group A than in Group B across a 24-h period. Importantly, the prevalence of ventricular tachycardia was significantly higher in Group A than in Group B (46% vs. 19%, P=0.04). Conclusions: SDB may induce cardiac electrical instability associated with life-threatening arrhythmias across a 24-h period in CHF.
  • Shinya Yamada, Hitoshi Suzuki, Masashi Kamioka, Satoshi Suzuki, Yoshiyuki Kamiyama, Akiomi Yoshihisa, Shu-ichi Saitoh, Yasuchika Takeishi  Circulation Journal  77-  (6)  1466  -1473  2013  [Not refereed][Not invited]
     
    Background: It has been shown that sleep-disordered breathing (SDB) is associated with adverse prognosis in patients with chronic heart failure (CHF), but little is known about the relationship between SDB and life-threatening arrhythmias. Methods and Results: Fifty patients with CHF and SDB (33 male mean age, 61 years) underwent Holter electrocardiogram and portable sleep monitoring simultaneously. The circadian variation in positive T-wave alternans (TWA > 65 μV) was determined during 6-h intervals (0-6, 6-12, 12-18, and 18-24 h). In addition, power spectral analysis of heart rate variability (HRV) was evaluated across a 24-h period. The subjects were divided into 2 groups based on whether respiratory disturbance index was ≥20 events/h (Group A, n=24) or not (Group B, n=26). The prevalence of positive TWA, parameters in HRV and the occurrence of ventricular tachycardia (> 5 beats) were compared between the 2 groups. The prevalence of positive TWA in Group A was significantly higher than that in Group B in all 6-h intervals. Low-frequency and high-frequency powers of HRV were significantly lower in Group A than in Group B across a 24-h period. Importantly, the prevalence of ventricular tachycardia was significantly higher in Group A than in Group B (46% vs. 19%, P=0.04). Conclusions: SDB may induce cardiac electrical instability associated with life-threatening arrhythmias across a 24-h period in CHF.
  • 藤宮 剛, 佐戸川弘之, 高瀬信弥, 三澤幸辰, 若松大樹, 黒澤博之, 瀬戸夕輝, 五十嵐崇, 籠島彰人, 横山 斉, 國井浩行, 上岡正志, 竹石恭知  心臓  45-  (7)  856  -857  2013  [Not refereed][Not invited]
  • Makiko Miyata, Akiomi Yoshihisa, Satoshi Suzuki, Shinya Yamada, Masashi Kamioka, Yoshiyuki Kamiyama, Takayoshi Yamaki, Koichi Sugimoto, Hiroyuki Kunii, Kazuhiko Nakazato, Hitoshi Suzuki, Shu-ichi Saitoh, Yasuchika Takeishi  JOURNAL OF CARDIOLOGY  60-  (3-4)  222  -227  2012/09  [Not refereed][Not invited]
     
    Background: Cheyne-Stokes respiration (CSR-CSA) is often observed in patients with chronic heart failure (CHF). Although cardiac resynchronization therapy (CRT) is effective for CHF patients with left ventricular dyssynchrony, it is still unclear whether adaptive servo ventilation (ASV) improves cardiac function and prognosis of CHF patients with CSR-CSA after CRT. Methods and results: Twenty two patients with CHF and CSR-CSA after CRT defibrillator (CRTD) implantation were enrolled in the present study and randomly assigned into two groups: 11 patients treated with ASV (ASV group) and 11 patients treated without ASV (non-ASV group). Measurement of plasma B-type natriuretic peptide (BNP) levels (before 3, and 6 months later) and echocardiography (before and 6 months) were performed in each group. Patients were followed up to register cardiac events (cardiac death and re-hospitalization) after discharge. In the ASV group, indices for apnea-hypopnea, central apnea, and oxyhemoglobin saturation were improved on ASV. BNP levels, cardiac systolic and diastolic function were improved with ASV treatment for 6 months. Importantly, the event-free rate was significantly higher in the ASV group than in the non-ASV group. Conclusions: ASV improves CSR-CSA, cardiac function, and prognosis in CHF patients with CRTD. Patients with CSR-CSA and post CRTD implantation would get benefits by treatment with ASV. (C) 2012 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.
  • Masashi Kamioka, Hitoshi Suzuki, Shinya Yamada, Yoshiyuki Kamiyama, Shu-ichi Saitoh, Yasuchika Takeishi  INTERNATIONAL HEART JOURNAL  53-  (5)  306  -312  2012/09  [Not refereed][Not invited]
     
    The purpose of this study was to determine whether high sensitivity C-reactive protein (hsCRP) before cardiac re-synchronization therapy (CRT) implantation was able to predict the response to CRT and cardiac deaths in severe heart failure patients. The study population consisted of 65 heart failure patients (46 males, mean age 65.0 +/- 11.8 years, NYHA class III/IV) with CRT implantation. Levels of hsCRP and B-type natriuretic peptide (BNP) were measured before CRT implantation. Left ventricular end-diastolic volume index (LVEDVI), left ventricular end-systolic volume index (LVESVI), and left ventricular ejection fraction (LVEF) were assessed by echocardiography at the same time. At 6 months after device implantation follow-up, echocardiography was performed and reverse remodeling was defined as > 15% reduction in LVESV. Of the 61 patients (4 patients died within 6 months), 41 patients (67%) and 20 patients (33%) were classified as responders (group-R) and nonresponders (group-NR), respectively. Cardiac deaths occurred more frequently in group-NR than in group-R (29% versus 5%, P < 0.05). Hs-CRP level was significantly higher in group-NR than in group-R (P < 0.01). Multivariate logistic regression analysis showed an independent relationship between hsCRP and the incidence of nonresponders (odds ratio: 1.499, P = 0.011). Stepwise multivariate Cox proportional hazard analysis identified the hsCRP level as the strongest predictive factor for cardiac death (hazard ratio: 1.337, P = 0.001). Receiver operating characteristic (ROC) analysis revealed hsCRP levels of 3.0 mg/L as the cut-off value for cardiac mortality. The hsCRP level may provide a new insight into CRT implantation for severe heart failure by predicting responses to CRT and cardiac death. (Int Heart J 2012; 53: 306-312)
  • Masashi Kamioka, Hitoshi Suzuki, Shinya Yamada, Yoshiyuki Kamiyama, Shu-ichi Saitoh, Yasuchika Takeishi  International Heart Journal  53-  (5)  306  -312  2012  [Not refereed][Not invited]
     
    The purpose of this study was to determine whether high sensitivity C-reactive protein (hsCRP) before cardiac resynchronization therapy (CRT) implantation was able to predict the response to CRT and cardiac deaths in severe heart failure patients. The study population consisted of 65 heart failure patients (46 males, mean age 65.0 ± 11.8 years, NYHA class III/IV) with CRT implantation. Levels of hsCRP and B-type natriuretic peptide (BNP) were measured before CRT implantation. Left ventricular end-diastolic volume index (LVEDVI), left ventricular end-systolic volume index (LVESVI), and left ventricular ejection fraction (LVEF) were assessed by echocardiography at the same time. At 6 months after device implantation follow-up, echocardiography was performed and reverse remodeling was defined as > 15% reduction in LVESV. Of the 61 patients (4 patients died within 6 months), 41 patients (67%) and 20 patients (33%) were classified as responders (group-R) and nonresponders (group-NR), respectively. Cardiac deaths occurred more frequently in group-NR than in group-R (29% versus 5%, P < 0.05). Hs-CRP level was significantly higher in group-NR than in group-R (P < 0.01). Multivariate logistic regression analysis showed an independent relationship between hsCRP and the incidence of nonresponders (odds ratio: 1.499, P = 0.011). Stepwise multivariate Cox proportional hazard analysis identified the hsCRP level as the strongest predictive factor for cardiac death (hazard ratio: 1.337, P = 0.001). Receiver operating characteristic (ROC) analysis revealed hsCRP levels of 3.0 mg/L as the cut-off value for cardiac mortality. The hsCRP level may provide a new insight into CRT implantation for severe heart failure by predicting responses to CRT and cardiac death.
  • YAMADA, Shinya, SUZUKI, Hitoshi, KAMIOKA, Masashi, KAMIYAMA, Yoshiyuki, SAITOH, Shu-ichi, TAKEISHI, Yasuchika  Fukushima Journal of Medical Science  58-  (2)  101  -106  2012  [Not refereed][Not invited]
  • MIYATA, Makiko, YOSHIHISA, Akiomi, SUZUKI, Satoshi, YAMADA, Shinya, KAMIOKA, Masashi, KAMIYAMA, Yoshiyuki, YAMAKI, Takayoshi, SUGIMOTO, Koichi, KUNII, Hiroyuki, NAKAZATO, Kazuhiko, SUZUKI, Hitoshi, SAITOH, Shu-ichi, TAKEISHI, Yasuchika  Journal of Cardiology  60-  (3)  222  -227  2012  [Not refereed][Not invited]
  • YAMADA, Shinya, SUZUKI, Hitoshi, KAMIOKA, Masashi, KAMIYAMA, Yoshiyuki, SAITOH, Shu-ichi, TAKEISHI, Yasuchika  Fukushima Journal of Medical Science  58-  (2)  101  -106  2012  [Not refereed][Not invited]
  • Masashi Kamioka, Toshiyuki Ishibashi, Hiroshi Ohkawara, Ryoji Nagai, Koichi Sugimoto, Hironori Uekita, Takanori Matsui, Sho-Ichi Yamagishi, Katsuya Ando, Takayuki Sakamoto, Nobuo Sakamoto, Yoh Takuwa, Ikuo Wada, Masashi Shiomi, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF CELLULAR PHYSIOLOGY  226-  (6)  1554  -1563  2011/06  [Not refereed][Not invited]
     
    An advanced glycation end products (AGE)/a receptor for AGE (RAGE) axis plays a key role in diabetic vascular complications. Membrane type 1-matrix metalloproteinase (MT1-MMP) has been shown to function not only as a proteolytic enzyme but also as a signaling molecule. In this study, we investigated the role of MT1-MMP in the AGE/RAGE-triggered signaling pathways in cultured rabbit smooth muscle cells (SMCs) and the molecular interaction between RAGE and MT1-MMP in vitro and in vivo. In SMCs, AGE-activated Rac1 and p47(phox) within 1 min, NADPH oxidase activity and reactive oxygen species (ROS) generation within 5 min, and NF-kappa B phosphorylation within 15 min, thereby inducing redox-sensitive molecular expression. Silencing of RAGE by small-interfering RNA (siRNA) blocked the AGE-induced signaling pathways. AGE-induced geranylgeranyl transferase I (GGTase I) activity, Rac1.p47(phox) activation, NADPH oxidase activity, ROS generation, and molecular expression were also markedly attenuated by silencing of MT1-MMP. An inhibitor of GGTase I mimicked the effects of MT1-MMP-specific siRNA. Fluorescent immunohistochemistry revealed that MT1-MMP was partially co-localized with RAGE in SMCs, and RAGE was found to form a complex with MT1-MMP in both cultured SMCs and the aortae of diabetic rats by immunoprecipitation. Furthermore, MT1-MMP and RAGE formed a complex in the aortic atherosclerotic lesions of hyperlipidemic rabbits. We show that MT1-MMP plays a crucial role in RAGE-activated NADPH oxidase-dependent signaling pathways and forms a complex with RAGE in the vasculature, thus suggesting that MT1-MMP may be a novel therapeutic target for diabetic vascular complications. J. Cell. Physiol. 226: 1554-1563, 2011. (C) 2010 Wiley-Liss, Inc.
  • Masashi Kamioka, Toshiyuki Ishibashi, Hiroshi Ohkawara, Ryoji Nagai, Koichi Sugimoto, Hironori Uekita, Takanori Matsui, Sho-Ichi Yamagishi, Katsuya Ando, Takayuki Sakamoto, Nobuo Sakamoto, Yoh Takuwa, Ikuo Wada, Masashi Shiomi, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF CELLULAR PHYSIOLOGY  226-  (6)  1554  -1563  2011/06  [Not refereed][Not invited]
     
    An advanced glycation end products (AGE)/a receptor for AGE (RAGE) axis plays a key role in diabetic vascular complications. Membrane type 1-matrix metalloproteinase (MT1-MMP) has been shown to function not only as a proteolytic enzyme but also as a signaling molecule. In this study, we investigated the role of MT1-MMP in the AGE/RAGE-triggered signaling pathways in cultured rabbit smooth muscle cells (SMCs) and the molecular interaction between RAGE and MT1-MMP in vitro and in vivo. In SMCs, AGE-activated Rac1 and p47(phox) within 1 min, NADPH oxidase activity and reactive oxygen species (ROS) generation within 5 min, and NF-kappa B phosphorylation within 15 min, thereby inducing redox-sensitive molecular expression. Silencing of RAGE by small-interfering RNA (siRNA) blocked the AGE-induced signaling pathways. AGE-induced geranylgeranyl transferase I (GGTase I) activity, Rac1.p47(phox) activation, NADPH oxidase activity, ROS generation, and molecular expression were also markedly attenuated by silencing of MT1-MMP. An inhibitor of GGTase I mimicked the effects of MT1-MMP-specific siRNA. Fluorescent immunohistochemistry revealed that MT1-MMP was partially co-localized with RAGE in SMCs, and RAGE was found to form a complex with MT1-MMP in both cultured SMCs and the aortae of diabetic rats by immunoprecipitation. Furthermore, MT1-MMP and RAGE formed a complex in the aortic atherosclerotic lesions of hyperlipidemic rabbits. We show that MT1-MMP plays a crucial role in RAGE-activated NADPH oxidase-dependent signaling pathways and forms a complex with RAGE in the vasculature, thus suggesting that MT1-MMP may be a novel therapeutic target for diabetic vascular complications. J. Cell. Physiol. 226: 1554-1563, 2011. (C) 2010 Wiley-Liss, Inc.
  • 佐藤友美, 上岡正志, 泉田次郎, 斎藤恒義, 斎藤富善, 前原和平, 鈴木 均, 竹石恭知  心臓  43-  (6)  760  -765  2011  [Not refereed][Not invited]
  • 今月の画像:致死性心室性不整脈を発症し蘇生された若年女性の1症例
    上岡正志, 國井浩行, 鈴木 均, 斎藤修一, 竹石恭知  日本心臓病学会誌  6-  (3)  246  -247  2011  [Not refereed][Not invited]
  • Hiroshi Ohkawara, Toshiyuki Ishibashi, Shu-ichi Saitoh, Nobutaka Inoue, Koichi Sugimoto, Masashi Kamioka, Hironori Uekita, Takashi Kaneshiro, Katsuya Ando, Yoh Takuwa, Yukio Maruyama, Yasuchika Takeishi  CARDIOVASCULAR RESEARCH  88-  (3)  492  -501  2010/12  [Not refereed][Not invited]
     
    Small GTPases RhoA and Rac1 play crucial roles in endothelial dysfunction and reactive oxygen species (ROS) generation. We reported evidence that in thrombin-stimulated endothelial cells, rapid geranylgeranylation is an essential process for full activation of unprocessed RhoA, which is blocked by statin. In this study, we examined the effects of intravenous administration of pravastatin on thrombin-triggered vascular responses in vivo, as well as on the lipid modification of unprocessed forms of RhoA and Rac1 and their activation induced by thrombin. Thrombin (50 U/kg) was intravenously injected with or without 0.3 mg/kg pravastatin into Wistar and spontaneously hypertensive rats. Coadministration of pravastatin prevented thrombin-induced impaired endothelium-dependent coronary vasodilation and down-regulated Akt/endothelial nitric oxide synthase (eNOS) phosphorylation within 1 h, as well as the down-regulation of eNOS protein expression within 4 h. In addition, thrombin increased Rac1/p47(phox)-dependent NAD(P)H oxidase activities of rat aortas within 1 h, resulting in ROS generation, which was prevented by the coadministration of pravastatin. Furthermore, the coadministration of pravastatin prevented thrombin-induced conversion of unprocessed RhoA and Rac1 into the geranylgeranylated forms as well as GTP-loading and membrane translocation within 1 h. Intravenous injection of pravastatin prevents impaired NO-dependent vasodilation and Rac1/NAD(P)H oxidase-mediated-ROS generation by blocking the down-regulation of Akt/eNOS pathways and the full activation of unprocessed RhoA and Rac1 in vivo.
  • Hiroshi Ohkawara, Toshiyuki Ishibashi, Shu-ichi Saitoh, Nobutaka Inoue, Koichi Sugimoto, Masashi Kamioka, Hironori Uekita, Takashi Kaneshiro, Katsuya Ando, Yoh Takuwa, Yukio Maruyama, Yasuchika Takeishi  CARDIOVASCULAR RESEARCH  88-  (3)  492  -501  2010/12  [Not refereed][Not invited]
     
    Small GTPases RhoA and Rac1 play crucial roles in endothelial dysfunction and reactive oxygen species (ROS) generation. We reported evidence that in thrombin-stimulated endothelial cells, rapid geranylgeranylation is an essential process for full activation of unprocessed RhoA, which is blocked by statin. In this study, we examined the effects of intravenous administration of pravastatin on thrombin-triggered vascular responses in vivo, as well as on the lipid modification of unprocessed forms of RhoA and Rac1 and their activation induced by thrombin. Thrombin (50 U/kg) was intravenously injected with or without 0.3 mg/kg pravastatin into Wistar and spontaneously hypertensive rats. Coadministration of pravastatin prevented thrombin-induced impaired endothelium-dependent coronary vasodilation and down-regulated Akt/endothelial nitric oxide synthase (eNOS) phosphorylation within 1 h, as well as the down-regulation of eNOS protein expression within 4 h. In addition, thrombin increased Rac1/p47(phox)-dependent NAD(P)H oxidase activities of rat aortas within 1 h, resulting in ROS generation, which was prevented by the coadministration of pravastatin. Furthermore, the coadministration of pravastatin prevented thrombin-induced conversion of unprocessed RhoA and Rac1 into the geranylgeranylated forms as well as GTP-loading and membrane translocation within 1 h. Intravenous injection of pravastatin prevents impaired NO-dependent vasodilation and Rac1/NAD(P)H oxidase-mediated-ROS generation by blocking the down-regulation of Akt/eNOS pathways and the full activation of unprocessed RhoA and Rac1 in vivo.
  • Taku Osugi, Shu-ichi Saitoh, Ken Matumoto, Mituru Muto, Kazuhiko Aikawa, Hiroshi Ohkawara, Kouichi Sugimoto, Masashi Kamioka, Toshiyuki Ishibashi, Yukio Maruyama  JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS  17-  (1)  54  -63  2010  [Not refereed][Not invited]
     
    Aim: We investigated the role of endothelin-1 (ET-1) in coronary microvascular spasm in a porcine model. Methods: A flowmeter was implanted around the left anterior descending coronary artery (LAD), and epicardial coronary artery endothelial denudation (ED) was performed just distal to the flowmeter every 2 weeks (W) until 6 W in 10 pigs (ED group). Ten pigs were chronically treated with endothelia type A receptor (ETA) antagonist (TA-0201, 0.1 mg/kg/day, ED + ETA group), while neither ED nor administration of ETA antagonist was performed in 12 pigs (Control group). We assessed changes in LAD blood flow and the denuded site diameter induced by acetylcholine (ACh, 0.05 mu g/kg i.c.). Results: In the ED group, administration of ACh to LAD induced zero flow without LAD diameter reduction at 8 W In the ED + ETA group, the decrease in LAD blood flow response to ACh was suppressed. Chronic administration of TA-0201 suppressed ROS generation in the myocardium. Decreases of eNOS and intimal thickening were smaller in the TA-0201 administration group than the non-TA-0201 administration group. Conclusion: Chronic administration of ETA receptor antagonist is effective to prevent coronary microvascular spasm.
  • Masashi Kamioka, Toshiyuki Ishibashi, Koichi Sugimoto, Hironori Uekita, Ryoji Nagai, Nobuo Sakamoto, Katsuya Ando, Hiroshi Ohkawara, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS  17-  (6)  590  -600  2010  [Not refereed][Not invited]
     
    Aim: Advanced glycation end products (AGE) and a receptor for AGE (RAGE) play a key role in diabetic vascular complications. Matrix metalloproteinases (MMPs) and apoptosis contribute to plaque instability. The renin-angiotensin system (RAS) is crucial for NADPH oxidase-dependent redox signaling pathways in the vascular wall. We investigated the effects of RAS blockade on AGE-triggered signaling pathways and its downstream events, including MMP-9 and apoptosis. Methods: We used cultured rabbit aortic smooth muscle cells (SMCs), which were stimulated with AGE in the presence or absence of temocaprilat or olmesartan. Results: Angiotensin converting enzyme (ACE) mRNA levels were increased 4 to 6 hours after adding AGE. AGE induced Rac1 and p47(phox) membrane translocation, reactive oxygen species (ROS) generation and NF-kappa B phosphorylation within 15 minutes, and various molecular expressions after 18 hours, which were attenuated by RAS blockade by temocaprilat or olmesartan. AGE-induced RAGE expression, as well as other molecules, including membrane type 1-MMP (MT1-MMP), monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1), was NADPH oxidase signaling-dependent and blunted by temocaprilat and olmesartan. The parameters of plaque instability, including MMP-9 expression and activity, and apoptosis were up-regulated by AGE, which was markedly attenuated by temocaprilat or olmesartan. Using isolated human monocyte culture, AGE-induced ROS generation and molecular expression were also attenuated by RAS blockade. Conclusion: The present study shows that AGE-triggered NADPH oxidase signaling pathways, including MMP-9 and apoptosis, were attenuated by RAS blockade, which may be an attractive strategy for treating plaque instability in diabetic vascular complications.
  • Taku Osugi, Shu-ichi Saitoh, Ken Matumoto, Mituru Muto, Kazuhiko Aikawa, Hiroshi Ohkawara, Kouichi Sugimoto, Masashi Kamioka, Toshiyuki Ishibashi, Yukio Maruyama  JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS  17-  (1)  54  -63  2010  [Not refereed][Not invited]
     
    Aim: We investigated the role of endothelin-1 (ET-1) in coronary microvascular spasm in a porcine model. Methods: A flowmeter was implanted around the left anterior descending coronary artery (LAD), and epicardial coronary artery endothelial denudation (ED) was performed just distal to the flowmeter every 2 weeks (W) until 6 W in 10 pigs (ED group). Ten pigs were chronically treated with endothelia type A receptor (ETA) antagonist (TA-0201, 0.1 mg/kg/day, ED + ETA group), while neither ED nor administration of ETA antagonist was performed in 12 pigs (Control group). We assessed changes in LAD blood flow and the denuded site diameter induced by acetylcholine (ACh, 0.05 mu g/kg i.c.). Results: In the ED group, administration of ACh to LAD induced zero flow without LAD diameter reduction at 8 W In the ED + ETA group, the decrease in LAD blood flow response to ACh was suppressed. Chronic administration of TA-0201 suppressed ROS generation in the myocardium. Decreases of eNOS and intimal thickening were smaller in the TA-0201 administration group than the non-TA-0201 administration group. Conclusion: Chronic administration of ETA receptor antagonist is effective to prevent coronary microvascular spasm.
  • Masashi Kamioka, Toshiyuki Ishibashi, Koichi Sugimoto, Hironori Uekita, Ryoji Nagai, Nobuo Sakamoto, Katsuya Ando, Hiroshi Ohkawara, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  Journal of Atherosclerosis and Thrombosis  17-  (6)  590  -600  2010  [Not refereed][Not invited]
     
    Aim: Advanced glycation end products (AGE) and a receptor for AGE (RAGE) play a key role in diabetic vascular complications. Matrix metalloproteinases (MMPs) and apoptosis contribute to plaque instability. The renin-angiotensin system (RAS) is crucial for NADPH oxidase-dependent redox signaling pathways in the vascular wall. We investigated the effects of RAS blockade on AGE-triggered signaling pathways and its downstream events, including MMP-9 and apoptosis. Methods: We used cultured rabbit aortic smooth muscle cells (SMCs), which were stimulated with AGE in the presence or absence of temocaprilat or olmesartan. Results: Angiotensin converting enzyme (ACE) mRNA levels were increased 4 to 6 hours after adding AGE. AGE induced Rac1 and p47phox membrane translocation, reactive oxygen species (ROS) generation and NF-κB phosphorylation within 15 minutes, and various molecular expressions after 18 hours, which were attenuated by RAS blockade by temocaprilat or olmesartan. AGE-induced RAGE expression, as well as other molecules, including membrane type 1-MMP (MT1-MMP), monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1), was NADPH oxidase signaling-dependent and blunted by temocaprilat and olmesartan. The parameters of plaque instability, including MMP-9 expression and activity, and apoptosis were up-regulated by AGE, which was markedly attenuated by temocaprilat or olmesartan. Using isolated human monocyte culture, AGE-induced ROS generation and molecular expression were also attenuated by RAS blockade. Conclusion: The present study shows that AGE-triggered NADPH oxidase signaling pathways, including MMP-9 and apoptosis, were attenuated by RAS blockade, which may be an attractive strategy for treating plaque instability in diabetic vascular complications.
  • Hiroshi Ohkawara, Toshiyuki Ishibashi, Masashi Shiomi, Koichi Sugimoto, Hironori Uekita, Masashi Kamioka, Yoh Takuwa, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS  16-  (6)  846  -856  2009/12  [Not refereed][Not invited]
     
    Aim: The activation of RhoA and Rac1 is crucial for the pathogenesis of atherosclerosis. This study investigated the changes of unprocessed and mature forms of RhoA and Rac1 in the progression of atherosclerosis. Methods: Unprocessed and geranylgeranylated forms of RhoA and Rac1 in aortic atherosclerotic lesions were separated by the Triton X-114 partition method using Watanabe heritable hyperlipidemic (WHHLMI) rabbits prone to myocardial infarction. The activation of RhoA and Rac1 was determined by membrane translocation and pull-down assays. Results: The levels of unprocessed RhoA and Rac1 of the aortas were higher at 7 months than 3 months, accompanied by increased levels of total RhoA and Rac1. Membrane-bound RhoA and Rac1 levels of the aortas at 7 months were significantly increased compared with those at 3 months, consistent with the results of GTP-loading. Unprocessed and activated forms of RhoA and Rac1 had gradually decreas at 15 and 24 months compared to 7 months. Conclusions: We show evidence of marked increases in unprocessed RhoA and Rac1 with enhanced activities in the progression of atherosclerosis in WHHLMI rabbits. This is important for better understanding of the pathogenesis of hyperlipidemia-dependent atherosclerosis.
  • Hiroshi Ohkawara, Toshiyuki Ishibashi, Masashi Shiomi, Koichi Sugimoto, Hironori Uekita, Masashi Kamioka, Yoh Takuwa, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS  16-  (6)  846  -856  2009/12  [Not refereed][Not invited]
     
    Aim: The activation of RhoA and Rac1 is crucial for the pathogenesis of atherosclerosis. This study investigated the changes of unprocessed and mature forms of RhoA and Rac1 in the progression of atherosclerosis. Methods: Unprocessed and geranylgeranylated forms of RhoA and Rac1 in aortic atherosclerotic lesions were separated by the Triton X-114 partition method using Watanabe heritable hyperlipidemic (WHHLMI) rabbits prone to myocardial infarction. The activation of RhoA and Rac1 was determined by membrane translocation and pull-down assays. Results: The levels of unprocessed RhoA and Rac1 of the aortas were higher at 7 months than 3 months, accompanied by increased levels of total RhoA and Rac1. Membrane-bound RhoA and Rac1 levels of the aortas at 7 months were significantly increased compared with those at 3 months, consistent with the results of GTP-loading. Unprocessed and activated forms of RhoA and Rac1 had gradually decreas at 15 and 24 months compared to 7 months. Conclusions: We show evidence of marked increases in unprocessed RhoA and Rac1 with enhanced activities in the progression of atherosclerosis in WHHLMI rabbits. This is important for better understanding of the pathogenesis of hyperlipidemia-dependent atherosclerosis.
  • Koichi Sugimoto, Toshiyuki Ishibashi, Tatsuya Sawamura, Nobutaka Inoue, Masashi Kamioka, Hironori Uekita, Hiroshi Ohkawara, Takayuki Sakamoto, Nobuo Sakamoto, Yasuo Okamoto, Yoh Takuwa, Akemi Kakino, Yoshiko Fujita, Takeshi Tanaka, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  CARDIOVASCULAR RESEARCH  84-  (1)  127  -136  2009/10  [Not refereed][Not invited]
     
    Aims RhoA and Rac1 activation plays a key role in endothelial dysfunction. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (ox-LDL) in endothelial cells (ECs). Membrane type 1 matrix metalloproteinase (MT1-MMP) has been shown to be involved in atherogenesis. This study was conducted to investigate the role of the LOX-1-MT1-MMP axis in RhoA and Rac1 activation in response to ox-LDL in ECs. Methods and results Ox-LDL induced rapid RhoA and Rac1 activation as well as MT1-MMP activity in cultured human aortic ECs. Inhibition of LOX-1 prevented ox-LDL-dependent RhoA and Rac1 activation. Knockdown of MT1-MMP by small interfering RNA prevented ox-LDL-induced RhoA and Rac1 activation, indicating that MT1-MMP is upstream of RhoA and Rac1. Fluorescent immunostaining revealed the colocalization of LOX-1 and MT1-MMP, and the formation of a complex of LOX-1 with MT1-MMP was detected by immunoprecipitation. Blockade of LOX-1 or MT1-MMP prevented RhoA-dependent endothelial NO synthase protein downregulation and cell invasion, Rac1-mediated NADPH oxidase activity, and reactive oxygen species generation. Conclusion The present study provides evidence that the LOX-1-MT1-MMP axis plays a crucial role in RhoA and Rac1 activation signalling pathways in ox-LDL stimulation, suggesting that this axis may be a promising target for treating endothelial dysfunction.
  • Koichi Sugimoto, Toshiyuki Ishibashi, Tatsuya Sawamura, Nobutaka Inoue, Masashi Kamioka, Hironori Uekita, Hiroshi Ohkawara, Takayuki Sakamoto, Nobuo Sakamoto, Yasuo Okamoto, Yoh Takuwa, Akemi Kakino, Yoshiko Fujita, Takeshi Tanaka, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  CARDIOVASCULAR RESEARCH  84-  (1)  127  -136  2009/10  [Not refereed][Not invited]
     
    Aims RhoA and Rac1 activation plays a key role in endothelial dysfunction. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (ox-LDL) in endothelial cells (ECs). Membrane type 1 matrix metalloproteinase (MT1-MMP) has been shown to be involved in atherogenesis. This study was conducted to investigate the role of the LOX-1-MT1-MMP axis in RhoA and Rac1 activation in response to ox-LDL in ECs. Methods and results Ox-LDL induced rapid RhoA and Rac1 activation as well as MT1-MMP activity in cultured human aortic ECs. Inhibition of LOX-1 prevented ox-LDL-dependent RhoA and Rac1 activation. Knockdown of MT1-MMP by small interfering RNA prevented ox-LDL-induced RhoA and Rac1 activation, indicating that MT1-MMP is upstream of RhoA and Rac1. Fluorescent immunostaining revealed the colocalization of LOX-1 and MT1-MMP, and the formation of a complex of LOX-1 with MT1-MMP was detected by immunoprecipitation. Blockade of LOX-1 or MT1-MMP prevented RhoA-dependent endothelial NO synthase protein downregulation and cell invasion, Rac1-mediated NADPH oxidase activity, and reactive oxygen species generation. Conclusion The present study provides evidence that the LOX-1-MT1-MMP axis plays a crucial role in RhoA and Rac1 activation signalling pathways in ox-LDL stimulation, suggesting that this axis may be a promising target for treating endothelial dysfunction.
  • Nobuo Sakamoto, Toshiyuki Ishibashi, Koichi Sugimoto, Tatsuya Sawamura, Takayuki Sakamoto, Nobutaka Inoue, Shu-Ichi Saitoh, Masashi Kamioka, Hironori Uekita, Hiroshi Ohkawara, Koji Suzuki, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF CELLULAR PHYSIOLOGY  220-  (3)  706  -715  2009/09  [Not refereed][Not invited]
     
    This study was conducted to examine the role of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in monocyte adhesion-induced redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in endothelial cells (ECs). LOX-1 was blocked by an antibody-neutralizing LOX-1 TS92 or small interfering RNA. In cultured human aortic ECs, monocyte adhesion activated Rac1 and p47(phox), and increased NADPH oxidase activity and reactive oxygen species (ROS) generation within 30 min and NF-kappa B phosphorylation within I h, resulting in redox-sensitive gene expression. Akt and eNOS phosphorylation was induced 15 min after adding monocytes and returned to control level after 30 min, whereas NO production was not altered by monocyte adhesion. Blockade of LOX-1 blunted the monocyte adhesion-triggered redox-sensitive signaling pathway and Akt/eNOS phosphorylation in ECs. Both endothelial intracellular Ca2+ mobilization and Ca2+ influx caused by monocyte attachment were markedly attenuated by pretreatment of ECs with TS92. This suggests that LOX-1 is involved in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of oxidized low-density lipoprotein (ox-LDL). Furthermore, blockade of Ca2+ inhibited monocyte adhesion-triggered Rac1 and p47(phox) activation and ROS generation in ECs, whereas Ca2+ signaling was suppressed by blockade of NADPH oxidase and ROS generation. Finally, TS92 blocked the monocyte adhesion to ECs stimulated with or without tumor necrosis factor-alpha or ox-LDL. We provide evidence that LOX-1 plays a role in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of the ox-LDL-LOX-1 axis. J. Cell. Physiol. 220: 706-715, 2009. (C) 2009 Wiley-Liss, Inc.
  • Shu-Ichi Saitoh, Ken Matsumoto, Masashi Kamioka, Hiroshi Ohkawara, Takashi Kaneshiro, Toshiyuki Ishibashi, Yukio Maruyama  CORONARY ARTERY DISEASE  20-  (6)  400  -408  2009/09  [Not refereed][Not invited]
     
    The role of endothelial dysfunction in coronary vasospasm is controversial. We hypothesized that reactive oxygen species (ROS) and endothelin-1 (ET-1) are plausible candidates as the mediator of vasospasm is linked to endothelial dysfunction. In a pig model with repetitive endothelial injury in coronary arteries, intracoronary administration of serotonin induced a vasospasm at the endothelial injury site. The level of endothelin-converting enzyme was upregulated at that site where, upon exposure to serotonin, there were also increases in p47(phox), ROS, and ET-1 fluorescence intensities, and myosin light chain phosphorylation and RhoA activation were detected. The nicotinamide adenine dinucleotide phosphate oxidase inhibitor, apocynin, had the effect of extinguishing not only ROS but also the appearance of ET-1. The chronic blockade of endothelin type-A receptor prevented a serotonin-triggered vasospasm along with the inhibition of ROS generation and myosin light chain phosphorylation. Under the coronary artery endothelial dysfunction, ET-1 is essential for an ROS-dependent coronary vasospasm. Our findings suggest that endothelial dysfunction plays a critical role in clinically defined human Prinzmetal angina. Coron Artery Dis 20:400-408 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
  • Nobuo Sakamoto, Toshiyuki Ishibashi, Koichi Sugimoto, Tatsuya Sawamura, Takayuki Sakamoto, Nobutaka Inoue, Shu-Ichi Saitoh, Masashi Kamioka, Hironori Uekita, Hiroshi Ohkawara, Koji Suzuki, Tamio Teramoto, Yukio Maruyama, Yasuchika Takeishi  JOURNAL OF CELLULAR PHYSIOLOGY  220-  (3)  706  -715  2009/09  [Not refereed][Not invited]
     
    This study was conducted to examine the role of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in monocyte adhesion-induced redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in endothelial cells (ECs). LOX-1 was blocked by an antibody-neutralizing LOX-1 TS92 or small interfering RNA. In cultured human aortic ECs, monocyte adhesion activated Rac1 and p47(phox), and increased NADPH oxidase activity and reactive oxygen species (ROS) generation within 30 min and NF-kappa B phosphorylation within I h, resulting in redox-sensitive gene expression. Akt and eNOS phosphorylation was induced 15 min after adding monocytes and returned to control level after 30 min, whereas NO production was not altered by monocyte adhesion. Blockade of LOX-1 blunted the monocyte adhesion-triggered redox-sensitive signaling pathway and Akt/eNOS phosphorylation in ECs. Both endothelial intracellular Ca2+ mobilization and Ca2+ influx caused by monocyte attachment were markedly attenuated by pretreatment of ECs with TS92. This suggests that LOX-1 is involved in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of oxidized low-density lipoprotein (ox-LDL). Furthermore, blockade of Ca2+ inhibited monocyte adhesion-triggered Rac1 and p47(phox) activation and ROS generation in ECs, whereas Ca2+ signaling was suppressed by blockade of NADPH oxidase and ROS generation. Finally, TS92 blocked the monocyte adhesion to ECs stimulated with or without tumor necrosis factor-alpha or ox-LDL. We provide evidence that LOX-1 plays a role in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of the ox-LDL-LOX-1 axis. J. Cell. Physiol. 220: 706-715, 2009. (C) 2009 Wiley-Liss, Inc.
  • Shu-Ichi Saitoh, Ken Matsumoto, Masashi Kamioka, Hiroshi Ohkawara, Takashi Kaneshiro, Toshiyuki Ishibashi, Yukio Maruyama  CORONARY ARTERY DISEASE  20-  (6)  400  -408  2009/09  [Not refereed][Not invited]
     
    The role of endothelial dysfunction in coronary vasospasm is controversial. We hypothesized that reactive oxygen species (ROS) and endothelin-1 (ET-1) are plausible candidates as the mediator of vasospasm is linked to endothelial dysfunction. In a pig model with repetitive endothelial injury in coronary arteries, intracoronary administration of serotonin induced a vasospasm at the endothelial injury site. The level of endothelin-converting enzyme was upregulated at that site where, upon exposure to serotonin, there were also increases in p47(phox), ROS, and ET-1 fluorescence intensities, and myosin light chain phosphorylation and RhoA activation were detected. The nicotinamide adenine dinucleotide phosphate oxidase inhibitor, apocynin, had the effect of extinguishing not only ROS but also the appearance of ET-1. The chronic blockade of endothelin type-A receptor prevented a serotonin-triggered vasospasm along with the inhibition of ROS generation and myosin light chain phosphorylation. Under the coronary artery endothelial dysfunction, ET-1 is essential for an ROS-dependent coronary vasospasm. Our findings suggest that endothelial dysfunction plays a critical role in clinically defined human Prinzmetal angina. Coron Artery Dis 20:400-408 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
  • Takayuki Sakamoto, Toshiyuki Ishibashi, Koichi Sugimoto, Nobuo Sakamoto, Hiroshi Ohkawara, Miki Niinuma, Kenji Nagataa, Masashi Kamioka, Naotoshi Sugimoto, Atai Watanabe, Masahiko Kurabayashi, Yoh Takuwa, Yukio Maruyama  ATHEROSCLEROSIS  193-  (1)  44  -54  2007/07  [Not refereed][Not invited]
     
    We investigated the role of RhoA activation and its mechanism in plasminogen activator inhibitor-1 (PAI-1) gene expression induced in endothelial cells by monocyte adhesion. Isolated human peripheral blood monocytes were added to cultured human coronary endothelial cells. Monocyte adhesion to endothelial cells increased PAI- 1 expression at the transcriptional level and activated RhoA which was accompanied by an increase in the activity of geranylgeranyl transferase I (GGTase 1), an enzyme responsible for geranylgeranylation, and actin stress fiber formation. Inhibition of RhoA by C3 exoenzyme or by adenovirus-mediated expression of N19RhoA, as well as by pravastatin, prevented the upregulation of PAI-1 induced by monocyte adhesion. GGTI-286, an inhibitor of GGTase 1, prevented the monocyte-induced RhoA activation and PAI- 1 expression in endothelial cells. Monocyte attachment induced an increase in intracellular Ca2+ concentration ([Ca (2+)],) in endothelial cells and Ca2+ chelation prevented the increased promoter activity and expression of PAI- 1 induced by monocyte adhesion. 0 exoenzyme and GGTI-286 also suppressed endothelial intracellular Ca2+ mobilization and Ca2+ entry induced by monocytes. The present study shows that GGTase I plays a role in the RhoA activation in endothelial cells induced by monocyte adhesion and that GGTase I-mediated Ca2+ signaling may contribute to RhoA-dependent PAI- 1 gene expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
  • Takayuki Sakamoto, Toshiyuki Ishibashi, Koichi Sugimoto, Nobuo Sakamoto, Hiroshi Ohkawara, Miki Niinuma, Kenji Nagataa, Masashi Kamioka, Naotoshi Sugimoto, Atai Watanabe, Masahiko Kurabayashi, Yoh Takuwa, Yukio Maruyama  ATHEROSCLEROSIS  193-  (1)  44  -54  2007/07  [Not refereed][Not invited]
     
    We investigated the role of RhoA activation and its mechanism in plasminogen activator inhibitor-1 (PAI-1) gene expression induced in endothelial cells by monocyte adhesion. Isolated human peripheral blood monocytes were added to cultured human coronary endothelial cells. Monocyte adhesion to endothelial cells increased PAI- 1 expression at the transcriptional level and activated RhoA which was accompanied by an increase in the activity of geranylgeranyl transferase I (GGTase 1), an enzyme responsible for geranylgeranylation, and actin stress fiber formation. Inhibition of RhoA by C3 exoenzyme or by adenovirus-mediated expression of N19RhoA, as well as by pravastatin, prevented the upregulation of PAI-1 induced by monocyte adhesion. GGTI-286, an inhibitor of GGTase 1, prevented the monocyte-induced RhoA activation and PAI- 1 expression in endothelial cells. Monocyte attachment induced an increase in intracellular Ca2+ concentration ([Ca (2+)],) in endothelial cells and Ca2+ chelation prevented the increased promoter activity and expression of PAI- 1 induced by monocyte adhesion. 0 exoenzyme and GGTI-286 also suppressed endothelial intracellular Ca2+ mobilization and Ca2+ entry induced by monocytes. The present study shows that GGTase I plays a role in the RhoA activation in endothelial cells induced by monocyte adhesion and that GGTase I-mediated Ca2+ signaling may contribute to RhoA-dependent PAI- 1 gene expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
  • Advanced glycation end products (AGEs, 終末糖化産物) 依存性遺伝子発現調節機構におけるMT1-MMP (膜型1マトリックスメタロプロテアーゼ) /RAGE会合の重要性
    上岡正志, 石橋敏幸, 丸山幸夫  血管  30-  (2)  61  -64  2007  [Not refereed][Not invited]

Awards & Honors

  • 2011 平成23年度福島県医師会 医学奨励賞
     JPN
  • 2006 日本心脈管作動物質学会研究奨励賞
     JPN


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