Researchers Database

miki atsushi

    SurgeryGastroenterological,GeneralandTransplantSurgery Assistant Professor
Contact: amikijichi.ac.jp
Last Updated :2021/12/04

Researcher Information

URL

Research funding number

  • 20570378

J-Global ID

Research Areas

  • Life sciences / Digestive surgery

Academic & Professional Experience

  • 2014/05 - Today  Jichi Medical University消化器一般移植外科講師
  • 2013  Jichi Medical UniversitySchool of Medicine助教
  • 2006/06 - 2009/07  University of MiamiDiabetes Research Institute Department of SurgeryResearch Associate

Education

  • 2003/04 - 2007/03  Okayama University  Graduate School of Medicine , Dentistry and Pharmaceutical Sciences
  • 1994/04 - 2000/03  Okayama University  Medical School

Published Papers

  • 高橋 礼, 笹沼 英紀, 池田 恵理子, 三木 厚, 森嶋 計, 吉田 淳, 遠藤 和洋, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 瓦井Lefor Alan, 福嶋 敬宜, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 36 (2) 142 - 149 0913-0071 2021/04 
    症例は37歳,女性.25歳で偶発的に膵体尾部に嚢胞性病変を指摘され,精査目的に当院消化器内科に紹介された.腹部造影CTとMRI検査では膵尾部に約5cmの嚢胞性腫瘤を認め膵粘液性嚢胞腫瘍(mucinous cystic neoplasm:MCN)が疑われたが,経過観察の方針となった.32歳時の第2子妊娠を契機に経過観察が一時中断となり,5年後の37歳に再診した.嚢胞性腫瘤は多房化し,大きさ6cmへの増大を認め,血清CA19-9の上昇を伴ったことから手術目的に消化器外科紹介となった.腹腔鏡下膵体尾部脾摘術を施行し,術後経過は良好で術後11日目に退院した.病理組織像では卵巣様間質を認め,微小浸潤を伴った膵粘液性嚢胞腺癌の診断であった.MCNの自然史を解明するには長期経過観察例の集積が必要である.(著者抄録)
  • 消化器外科用新規接着剤の開発
    佐久間 康成, 田口 哲志, 三木 厚, 斉藤 晶, 芝 聡美, 堀江 久永, 細谷 好則, 山口 博紀, 川平 洋, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 120回 DP - 8 2020/08
  • 肝門部領域胆管癌における切除断端と再発形態についての検討
    森嶋 計, 下平 健太郎, 青木 裕一, 三木 厚, 宮戸 英世, 遠藤 和洋, 吉田 淳, 笹沼 英紀, 山口 博紀, 佐久間 康成, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 120回 DP - 5 2020/08
  • 田口 昌延, 佐久間 康成, 小泉 大, 笹沼 英紀, 下平 健太郎, 齋藤 晶, 木村 有希, 青木 裕一, 目黒 由行, 笠原 尚哉, 森嶋 計, 兼田 裕司, 三木 厚, 宮戸 英世, 遠藤 和洋, 吉田 淳, 清水 敦, 山口 博紀, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 35 (3) A337 - A337 0913-0071 2020/07
  • 切除可能膵癌に対する術前化学放射線療法の有効性
    三木 厚, 佐久間 康成, 笹沼 英紀, 遠藤 和洋, 吉田 淳, 森嶋 計, 下平 健太郎, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 35 (3) A281 - A281 0913-0071 2020/07
  • 切除可能膵癌に対する術前化学放射線療法の有効性
    三木 厚, 佐久間 康成, 笹沼 英紀, 遠藤 和洋, 吉田 淳, 森嶋 計, 下平 健太郎, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 35 (3) A281 - A281 0913-0071 2020/07
  • Heidelberg大学病院の膵頭十二指腸切除術
    田口 昌延, 佐久間 康成, 小泉 大, 笹沼 英紀, 下平 健太郎, 齋藤 晶, 木村 有希, 青木 裕一, 目黒 由行, 笠原 尚哉, 森嶋 計, 兼田 裕司, 三木 厚, 宮戸 英世, 遠藤 和洋, 吉田 淳, 清水 敦, 山口 博紀, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 35 (3) A337 - A337 0913-0071 2020/07
  • 大豆生田 尚彦, 森嶋 計, 宮戸 秀世, 片野 匠, 篠原 翔一, 兼田 裕司, 遠藤 和洋, 三木 厚, 吉田 淳, 笹沼 英紀, 山口 博紀, 佐久間 康成, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏
    日本内分泌学会雑誌 (一社)日本内分泌学会 95 (4) 1697 - 1697 0029-0661 2020/02
  • 上腸間膜動脈(SMA)急性閉塞症において大量腸切除を回避するための初期治療戦略に関する全国調査
    中尾 祐樹, 笹沼 英紀, 吉田 淳, 遠藤 和洋, 三木 厚, 森嶋 計, 田口 昌延, 佐久間 康成, 佐田 尚宏
    日本腹部救急医学会雑誌 (一社)日本腹部救急医学会 40 (2) 259 - 259 1340-2242 2020/02
  • 肝内胆管癌疑いの症例に対して腹腔鏡下肝切除を行い肝類上皮細胞肉芽腫と診断された1例
    吉田 淳, 佐久間 康成, 宮戸 英世, 森嶋 計, 三木 厚, 兼田 裕司, 遠藤 和洋, 笹沼 英紀, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏
    日本内視鏡外科学会雑誌 (一社)日本内視鏡外科学会 24 (7) MO188 - 5 1344-6703 2019/12
  • 佐久間 康成, 寺谷 工, 笠原 尚哉, 三木 厚, 春田 英律, 細谷 好則, 北山 丈二, 佐田 尚宏
    肥満研究 (一社)日本肥満学会 25 (Suppl.) 302 - 302 1343-229X 2019/10
  • リウマチに対してメトトレキサート内服中に発症した、肝原発悪性リンパ腫の一例
    大豆生田 尚彦, 森嶋 計, 宮戸 秀世, 遠藤 和洋, 三木 厚, 吉田 厚, 笹沼 英紀, 山口 博紀, 佐久間 康成, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 80 (増刊) 785 - 785 1345-2843 2019/10
  • Satomi Shiba, Atsushi Miki, Hideyuki Ohzawa, Takumi Teratani, Yasunaru Sakuma, Alan Kawarai Lefor, Joji Kitayama, Naohiro Sata
    The Journal of surgical research 238 79 - 89 2019/06 
    OBJECTIVE: Mucin1 (MUC1), a member of the mucin family, is a glycoprotein which is often expressed in malignant cells. However, the expression and function of MUC1 in human duodenal adenocarcinoma (DAC) has not yet been characterized because of its low frequency. Here, we examined the functional roles of core protein (MUC1-C) in DAC. MATERIALS AND METHODS: Using a human duodenal cancer cell line, HuTu80, proliferation, migration, invasion, ALDH activity was assessed by cell counting kit-8, scratch wound healing, matrigel invasion, and ALDEFUOR assays, respectively. The function of MUC1 protein was evaluated with knockdown using specific siRNA as well as anti-MUC1-C peptide, GO203. MUC1 expression in human DAC was evaluated immunohistochemically in surgically resected tumors. RESULTS: The positive expression of MUC1 in HuTu80 was confirmed by RT-PCR and flow cytometry. In vitro cell growth was inhibited by the addition of 50-100 μM GO203 as well as treatment with siRNA for MUC1-C. Silencing of MUC1-C also significantly reduced migration, invasion, ALDH activity. Local injection of GO-203 (14 mg/kg) significantly suppressed the growth of subcutaneous HuTu80 tumors in nude mice. Immunohistochemically, MUC1 was strongly detected in seven DAC cases, but not in 11 others. The outcome of patients with high MUC1 expression was significantly worse than those without MUC1 expression. CONCLUSIONS: These results suggest that MUC1 is functionally associated with the malignant potential of DAC and could be a novel therapeutic target for this rare tumor.
  • 当科における術前静脈血栓塞栓症(VTE)スクリーニングの有用性
    清水 徹一郎, 窓岩 清治, 堀江 久永, 斎藤 晶, 芝 聡美, 三木 厚, 佐久間 康成, 細谷 好則, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 119回 PS - 1 2019/04
  • 腹腔動脈狭窄を伴う特発性下膵十二指腸動脈破裂の1例
    小泉 恵, 吉田 淳, 高橋 和也, 青木 裕一, 田口 昌延, 森嶋 計, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 80 (3) 630 - 630 1345-2843 2019/03
  • 鼠径部ヘルニア嵌頓症例における経過時間と腸切除の検討
    小泉 大, 斎藤 晶, 青木 裕一, 森嶋 計, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 吉田 淳, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本腹部救急医学会雑誌 (一社)日本腹部救急医学会 39 (2) 379 - 379 1340-2242 2019/02
  • 膵十二指腸動脈瘤の3例
    渡部 晃佑, 吉田 淳, 高橋 和也, 青木 裕一, 田口 昌延, 森嶋 計, 三木 厚, 兼田 裕司, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本腹部救急医学会雑誌 (一社)日本腹部救急医学会 39 (2) 345 - 345 1340-2242 2019/02
  • 複雑な部分内臓逆位症を伴う総胆管結石症に対し手術を行った1例
    丸藤 琴音, 吉田 淳, 笹沼 英紀, 宮戸 秀世, 目黒 由行, 森嶋 計, 三木 厚, 遠藤 和洋, 小泉 大, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 79 (増刊) 852 - 852 1345-2843 2018/10
  • 原発性十二指腸癌におけるムチンタンパク発現と悪性化機序解明(Functional blockade of MUC1 can inhibit the progression of duodenum adenocarcinoma)
    芝 聡美, 三木 厚, 大澤 英之, 寺谷 工, 佐久間 康成, アラン・レフォー, 北山 丈二, 佐田 尚宏
    日本癌学会総会記事 77回 2174 - 2174 0546-0476 2018/09
  • 【ここまで来た ナビゲーション手術】胆道癌、膵癌に対するナビゲーション手術 三次元モデルによる術前シミュレーション、術中ナビゲーションおよびシミュレーション・トレーニング
    遠藤 和洋, 佐田 尚宏, 太白 健一, 木村 有希, 青木 裕一, 田口 昌延, 森嶋 計, 三木 厚, 吉田 淳, 小泉 大, 笹沼 英紀, 佐久間 康成
    消化器外科 (株)へるす出版 41 (9) 1317 - 1327 0387-2645 2018/08
  • 東條 峰之, 小泉 大, 齋藤 晶, 目黒 由行, 田口 昌延, 三木 厚, 宮戸 秀世, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 佐田 尚宏
    胆道 日本胆道学会 32 (3) 588 - 588 0914-0077 2018/08
  • 下行結腸癌異時性孤立性脾転移の1例
    松宮 美沙希, 三木 厚, 川口 英之, 兼田 裕司, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 79 (7) 1556 - 1556 1345-2843 2018/07
  • 膵体尾部切除術における膵断端処理と術後膵液瘻の検討
    木村 有希, 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 73回 1090 - 1090 2018/07
  • 術後膵液瘻ゼロを目指した膵空腸吻合の工夫 Blumgart変法自治医大方式
    小泉 大, 田口 昌延, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 73回 374 - 374 2018/07
  • 原発性副甲状腺機能亢進症に併発した重症急性膵炎後の感染性被包化壊死の1例
    辻 賢太郎, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 79 (6) 1352 - 1353 1345-2843 2018/06
  • 小泉 大, 斎藤 晶, 目黒 由行, 宮戸 秀世, 田口 昌延, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 33 (3) 641 - 641 0913-0071 2018/05
  • 笹沼 英紀, 佐田 尚宏, 田口 昌延, 三木 厚, 遠藤 和洋, 小泉 大, 佐久間 康成
    膵臓 (一社)日本膵臓学会 33 (3) 678 - 678 0913-0071 2018/05
  • 三木 厚, 佐久間 康成, 斉藤 晶, 目黒 由行, 遠藤 和洋, 小泉 大, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 33 (3) 415 - 415 0913-0071 2018/05
  • 泉 遼, 遠藤 和洋, 斉藤 晶, 田口 昌延, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 33 (3) 662 - 662 0913-0071 2018/05
  • 齋藤 匠, 笹沼 英紀, 田口 昌延, 三木 厚, 遠藤 和洋, 小泉 大, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 33 (3) 684 - 684 0913-0071 2018/05
  • 拡大肝切除後合併症のリスクファクターの検討
    三木 厚, 佐久間 康成, 遠藤 和洋, 小泉 大, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 118回 1682 - 1682 2018/04
  • 膵頭十二指腸切除術を施行した十二指腸乳頭部癌の長期成績
    小泉 大, 斎藤 晶, 横田 真一郎, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 118回 2414 - 2414 2018/04
  • 胃癌切除症例における術前D-dimer値と病期・転帰の検討
    倉科 憲太郎, 細谷 好則, 清水 徹一郎, 太田 学, 齋藤 心, 春田 英律, 宇井 崇, 松本 志郎, 金丸 理人, 三木 厚, 山口 博紀, 堀江 久永, 佐久間 康成, 北山 丈二, 佐田 尚宏
    日本胃癌学会総会記事 (一社)日本胃癌学会 90回 499 - 499 2018/03
  • 太田 学, 三木 厚, 遠藤 和洋, 佐久間 康成, アラン K.・レフォー, 佐田 尚宏
    日本臨床外科学会雑誌 日本臨床外科学会 79 (2) 405 - 411 1345-2843 2018/02 
    症例は44歳,女性.主訴は心窩部不快感.CTで膵尾部に径約70mmの嚢胞性病変,それと連続し胃壁内に小嚢胞の集簇を認めた.経過観察中に突然の腹痛で当院へ救急搬送され,CTで胃側の嚢胞が長径95mmと増大しており入院した.超音波内視鏡検査で胃側の嚢胞は胃壁筋層内に存在し,膵尾部の嚢胞との交通を認めた.穿刺内容液は血性で,内容液中のamylase(AMY),CEAが高値であった.膵粘液性嚢胞腫瘍(MCN)が胃壁内へ穿破したことによる胃壁筋層内出血と診断し,待機的に手術を行った.手術は膵体尾部脾合併切除を行い,胃側の嚢胞は胃壁の筋層を温存し嚢胞壁のみ合併切除した.膵嚢胞内容液のAMY,CEAは高値で胃壁側の嚢胞液と類似していた.病理組織診断ではMCNの診断であった.MCNが膵管と交通を持ち,胃壁筋層内へ穿破し出血を生じたと考えられる1例を経験したため,文献的考察も含めて報告する.(著者抄録)
  • Atsushi Miki, Camillo Ricordi, Yasunaru Sakuma, Toshiyuki Yamamoto, Ryosuke Misawa, Atsuyoshi Mita, Ruth D Molano, Nosratola D Vaziri, Antonello Pileggi, Hirohito Ichii
    PloS one 13 (5) e0196570  2018 
    BACKGROUND: Type 1 and Type 2 diabetes mellitus (T1DM and T2DM) are caused by beta(β)-cell loss and functional impairment. Identification of mechanisms of β-cell death and therapeutic interventions to enhance β-cell survival are essential for prevention and treatment of diabetes. Oxidative stress is a common feature of both T1DM and T2DM; elevated biomarkers of oxidative stress are detected in blood, urine and tissues including pancreas of patients with DM. Islet transplantation is a promising treatment for diabetes. However, exposure to stress (chemical and mechanical) and ischemia-reperfusion during isolation and transplantation causes islet loss by generation of reactive oxygen species (ROS). Human intracellular antioxidant enzymes and related molecules are essential defenses against ROS. Antioxidant enzyme levels including superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) have been shown to be low in islet cells. However, little is known about the expression and function of antioxidant enzymes within islet cell subsets. We evaluated the expression of the key antioxidant enzymes in β- and alpha(α)-cell and accessed effects of oxidative stress, islet isolation and transplantation on β/α-cell ratio and viability in human islets. METHODS: Human pancreata from T1DM, T2DM and non-diabetic deceased donors were obtained and analyzed by confocal microscopy. Isolated islets were (I) transplanted in the renal sub-capsular space of streptozotocin-induced diabetic nude mice (in vivo bioassay), or (II) exposed to oxidative (H2O2) and nitrosative (NO donor) stress for 24 hrs in vitro. The ratio, % viability and death of β- and α-cells, and DNA damage (8OHdG) were measured. RESULTS AND CONCLUSIONS: Catalase and GPX expression was much lower in β- than α-cells. The β/α-cell ratio fells significantly following islet isolation and transplantation. Exposure to oxidative stress caused a significantly lower survival and viability, with higher DNA damage in β- than α-cells. These findings identified the weakness of β-cell antioxidant capacity as a main cause of vulnerability to oxidative stress. Potential strategies to enhance β-cell antioxidant capacity might be effective in prevention/treatment of diabetes.
  • 笹沼 英紀, 佐田 尚宏, 小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 佐久間 康成, 堀江 久永, 細谷 好則, 瓦井 Lefor Alan
    小切開・鏡視外科学会雑誌 = Journal of Lift Endoscopy & Minimal Incision Surgery : 小切開・鏡視外科学会誌 小切開・鏡視外科学会 9 (1) 47 - 51 2185-2820 2018 [Refereed][Not invited]
     
    症例1は30歳男性で、発熱と左腹部痛で来院した。CTで感染性Walled-off necrosis(WON)と診断し、保存的治療を行ったが、症状ならびに炎症所見の改善を認めず、インターベンション治療目的に当院転院となった。造影CT所見から壊死物質を主成分とした感染性WONと診断し、小切開Videoscope補助下に後腹膜経路でネクロセクトミーを行った。1回のネクロセクトミーで炎症所見とCT所見は速やかに改善し、追加の処置は不要となった。左後腹膜のドレーンは留置したまま入院40日目に退院した。症例2は34歳男性で、急性膵炎の診断で保存的治療が開始され、退院約2週間後に間歇的腹痛と発熱で来院し、炎症反応高値、CTで感染性WONと診断され再入院した。造影CT所見から膵体尾部の壊死物質を主成分とする左側感染性WONと診断し、腹腔経路で小切開Videoscope補助下にネクロセクトミーを行った。その後、右側WON増大による閉塞性黄疸が出現し、開腹ネクロセクトミーと胆道ドレナージが必要となった。症状出現から7ヵ月後、ERCPで左右肝管にERBD tubeを留置し退院した。
  • 胃癌腹膜播種治療におけるバイオマーカーとしての腹水中エクソソームマイクロRNA解析
    大澤 英之, 三木 厚, 山口 博紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本癌学会総会記事 76回 P - 3240 0546-0476 2017/09
  • 佐田 尚宏, 齋藤 晶, 木村 有希, 青木 裕一, 田口 昌延, 笠原 尚哉, 森嶋 計, 三木 厚, 兼田 裕司, 遠藤 和洋, 小泉 大, 笹沼 英紀, 吉田 淳, 清水 敦, 佐久間 康成, 栗原 克己, 大木 準, 鈴木 正徳
    臨床外科 = Journal of clinical surgery 医学書院 72 (8) 986 - 989 0386-9857 2017/08 [Refereed][Not invited]
  • 胃切除後の胆道結石症の検討
    木村 有希, 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 72回 PP2 - 1 2017/07
  • 当科における膵IPMNの縮小手術の検討
    齋藤 匠, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 72回 PK4 - 6 2017/07
  • 退形成性膵管癌の6切除例
    風當 ゆりえ, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 72回 PL4 - 1 2017/07
  • 齋藤 匠, 三木 厚, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    膵臓 (一社)日本膵臓学会 32 (3) 460 - 460 0913-0071 2017/05
  • 質感と人体解剖を忠実に再現した、高難度手術教育のための臓器モデル 鏡視下手術の教育効果を高めるために必要な技術トレーニング
    遠藤 和洋, 太白 健一, 笠原 尚哉, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 117回 SF - 4 2017/04
  • 膵空腸吻合Blumgart変法(自治医大方式)に適した膵臓の検討
    小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, Lefor Alan, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 117回 PS - 1 2017/04
  • IPMN国際診療ガイドラインからみた当院でのIPMN手術例の検討
    齋藤 匠, 三木 厚, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 117回 PS - 4 2017/04
  • 家族性大腸腺腫症に合併した十二指腸病変の検討
    木村 有希, 小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 117回 PS - 7 2017/04
  • Hideyuki Ohzawa, Atsushi Miki, Takumi Teratani, Satomi Shiba, Yasunaru Sakuma, Wataru Nishimura, Yasuko Noda, Noriyoshi Fukushima, Hirofumi Fujii, Yasuo Hozumi, Hirofumi Mukai, Yoshikazu Yasuda
    Oncology letters 13 (3) 1731 - 1740 2017/03 
    Pathological complete response (pCR) is considered to be a useful prognostic marker for neoadjuvant chemotherapy to improve the survival rate of patients with operable breast cancer. In the present study, we identified differentially expressed microRNAs (miRNAs) between pCR and non-pCR groups of patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer who received neoadjuvant chemotherapy with trastuzumab. Expression profiles were examined by miRNA microarrays using total RNA extracted from formalin-fixed, paraffin-embedded tissues from pretreatment biopsy specimens. Significant differences were observed in miRNAs associated with pCR between the luminal B-like (HER2-positive) and HER2-positive (nonluminal) subtypes, which were further classified according to their estrogen receptor (ER) status. Prediction models constructed with differentially expressed miRNAs performed well. In conclusion, the combination of miRNA profiles and ER status may improve the accuracy of pCR prediction in patients with HER2-positive breast cancer and enable the development of personalized treatment regimens.
  • 佐田尚宏, 齋藤晶, 木村有希, 青木裕一, 田口昌延, 笠原尚哉, 森嶋計, 三木厚, 兼田裕司, 遠藤和洋, 小泉大, 笹沼英紀, 佐久間康成, 栗原克己, 大木準, 鈴木正徳
    胆とすい 38 (2) 195‐199  0388-9408 2017/02 [Not refereed][Not invited]
  • 膵体尾部を中心とした左側感染性WONに対する治療戦略
    笹沼 英紀, 佐久間 康成, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本腹部救急医学会雑誌 (一社)日本腹部救急医学会 37 (2) 292 - 292 1340-2242 2017/02
  • Yasunaru Sakuma, Yoshikazu Yasuda, Naohiro Sata, Yoshinori Hosoya, Atsushi Shimizu, Hirofumi Fujii, Daisuke Matsubara, Noriyuki Fukushima, Atsushi Miki, Misato Maeno, Alan Kawarai Lefor
    BMC cancer 17 (1) 37 - 37 2017/01 
    BACKGROUND: Long-term term survival in patients with pancreatic neuroendocrine tumors has been reported, even in patients with metastatic disease. Metastases to the spleen are extremely rare, but have been reported from a number of primary malignancies, such as breast cancer, lung cancer, melanoma and ovarian cancer. This is the first report of a splenic metastasis from a primary pancreatic neuroendocrine tumor. CASE PRESENTATION: The patient presented as a 53 years old white male with anemia and fatigue. Physical examination revealed a left upper quadrant fullness and computed tomography showed a 24 cm left upper quadrant mass with multiple liver metastases, splenomegaly and a 1 cm mass in the spleen. Resection of the primary pancreatic tumor (T4N0M1) was accompanied by gastrectomy, splenectomy and resection of adherent bowel. The spleen contained a metastatic lesion 1.0 cm in diameter, consistent with a primary neuroendocrine tumor of the pancreas. This operation was followed 8 months later, by delayed resection of liver metastases. The patient receives monthly administration of somatostatin long-acting analogue and has undergone several ablations of liver lesions with percutaneous radiofrequency ablation as well as a second liver resection. The patient is alive seven years after initial presentation, with no evidence of disease on imaging studies. CONCLUSIONS: This is the first report of a splenic metastasis from a primary pancreatic neuroendocrine tumor. The patient initially presented with synchronous multiple liver metastases and a single splenic metastasis. After resection of the primary tumor and spleen, the patient has undergone aggressive cytoreductive surgery/ablation of liver lesions and somatostatin therapy with resulting long-term survival.
  • 木村 有希, 三木 厚, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北谷 丈二, 佐田 尚宏
    胆膵の病態生理 = The Japanese journal of bilio-pancreatic pathophysiology 日本胆膵病態・生理研究会 33 (1) 43 - 46 2185-4564 2017 [Refereed][Not invited]
  • 佐田尚宏, 齋藤晶, 青木裕一, 木村有希, 田口昌延, 笠原尚哉, 森嶋計, 兼田裕司, 三木厚, 遠藤和洋, 小泉大, 笹沼英紀, 吉田淳, 清水敦, 佐久間康成
    胆とすい 37 (12) 1575‐1579  0388-9408 2016/12 [Not refereed][Not invited]
  • 医工連携による革新的内視鏡手術トレーニングシステムの開発 外科教育の新たな手法確立に向けて
    遠藤 和洋, 太白 健一, 笠原 直哉, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏
    日本内視鏡外科学会雑誌 (一社)日本内視鏡外科学会 21 (7) OS145 - 6 1344-6703 2016/12
  • 腹腔鏡下肝切除にRFAを併用して治療した肝細胞癌4例の経験
    森嶋 計, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本内視鏡外科学会雑誌 (一社)日本内視鏡外科学会 21 (7) DP99 - 1 1344-6703 2016/12
  • 佐田尚宏, 遠藤和洋, 兼田裕司, 笠原尚哉, 森嶋計, 三木厚, 小泉大, 笹沼英紀, 佐久間康成
    消化器外科 (株)へるす出版 39 (10) 1347‐1359 - 1359 0387-2645 2016/09 [Not refereed][Not invited]
  • 消化器外科手術アトラス Blumgart-dumpling法膵空腸吻合を併置した幽門輪温存膵頭十二指腸切除術
    佐田 尚宏, 遠藤 和洋, 兼田 裕司, 笠原 尚哉, 森嶋 計, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成
    消化器外科 (株)へるす出版 39 (10) 1347 - 1359 0387-2645 2016/09 [Not refereed][Not invited]
  • 外科における基礎的研究 セレンディピティーを求めて 短腸症モデルラットを用いたポリアミン摂取による治療効果の検討と小腸再生促進メカニズムの解明
    笠原 尚哉, 寺谷 工, 横田 真一郎, 三木 厚, 浦橋 泰然, 藤本 康弘, 佐久間 康成, 佐田 尚宏
    日本消化器外科学会総会 (一社)日本消化器外科学会 71回 WS7 - 1 2016/07
  • 転移性肝癌との鑑別に苦慮した肝柵状肉芽腫の一例
    森本 光昭, 佐久間 康成, 笹沼 秀紀, 小泉 大, 遠藤 和洋, 三木 厚, 北山 丈二, アラン・レフォー, 佐田 尚宏
    日本肝胆膵外科学会・学術集会プログラム・抄録集 (一社)日本肝胆膵外科学会 28回 565 - 565 2016/06
  • 膵lymphoepithelial cystの一切除例
    湯澤 真梨子, 三木 厚, 利府 和馬, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    栃木県医学会々誌 栃木県医学会 46 49 - 51 0285-6387 2016/06
  • 非黄疸胆道閉鎖症における肝移植適応
    眞田 幸弘, 浦橋 泰然, 井原 欣幸, 山田 直也, 岡田 憲樹, 平田 雄大, 片野 匠, 三木 厚, 笹沼 英紀, 佐久間 康成, 佐田 尚宏, 水田 耕一
    日本肝胆膵外科学会・学術集会プログラム・抄録集 (一社)日本肝胆膵外科学会 28回 473 - 473 2016/06
  • 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 佐田 尚宏
    手術 金原出版(株) 70 (7) 985 - 989 0037-4423 2016/06 [Not refereed][Not invited]
  • Y. Sakuma, H. Sasanuma, A. Miki, A. Shimizu, N. Sata, Y. Yasuda, A. K. Lefor, Y. Hirata, N. Yamada, N. Okada, Y. Sanada, Y. Ihara, T. Urahashi, K. Mizuta
    Transplantation Proceedings 48 (4) 1110 - 1114 1873-2623 2016/05 [Refereed][Not invited]
     
    Background In small infants, left lateral segment grafts are sometimes too large to overcome the problems of large-for-size grafts in the abdominal compartment. To address this problem, we have developed a safe living donor graftectomy for neonates, a so-called "S2 monosegment graft" to minimize graft thickness. We reviewed our single-center experience to evaluate the feasibility of this technique for reducing graft size. Methods Eleven living-donor liver transplants using S2 monosegment grafts were performed between October 2008 and September 2014 at our institution. Medical records of both donors and recipients were reviewed and data collected retrospectively. Results The mean age of recipients at the time of transplantation was 125.3 days, including 3 neonates. The average S2 monosegment graft weight was 127.4 g, and the graft-to-recipient body weight ratio was successfully reduced to 3.5%. The graft livers were reduced to 4.1 cm in thickness. Two recipients with grafts larger than 5 cm could not undergo primary abdominal closure. Portal vein stenosis and biliary stenosis was observed in 1 recipient, and hepatic artery complications were seen in 2 recipients the clinical course for all donors were uneventful. Liver regeneration was seen in every patient. The graft and patient 1-year survival rate was 100%. Conclusions Living-donor liver transplantation using S2 monosegment grafts offers a safe and useful option for treating smaller infants. Here, we introduce our method of S2 monosegment graft emphasizing the donor harvest and graft thickness.
  • 十二指腸乳頭部癌に対する膵頭十二指腸切除術の長期成績の検討
    小泉 大, 田口 昌延, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 116回 PS - 4 2016/04
  • 乳癌術後経過観察中に発見し、診断に難渋した異時性重複癌の1例
    湯澤 真梨子, 三木 厚, 利府 数馬, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 116回 RS - 5 2016/04
  • K Nanmoku, N Ishikawa, A Kurosawa, T Shimizu, T Kimura, A Miki, Y Sakuma, T Yagisawa
    Transplant infectious disease : an official journal of the Transplantation Society 18 (2) 234 - 9 2016/04 
    BACKGROUND: Urinary tract infection caused by human adenovirus (HAdV) after renal transplantation (RT) results in graft loss because of concomitant nephropathy and acute rejection and may result in death because of systemic dissemination. METHODS: We assessed the time period between RT and disease onset, symptoms, treatment details, disease duration, renal graft function, outcomes, and complications. RESULTS: HAdV infection of the urinary tract occurred in 8 of 170 renal transplant recipients. Symptoms were macrohematuria in all 8 patients, dysuria in 7, and fever in 5. The median period from RT to disease onset was 367 (range, 7-1763) days, and the median disease duration was 15 (range, 8-42) days. The mean serum creatinine (sCr) level prior to onset was 1.35 ± 0.48 mg/dL and the mean maximum sCr level during disease was 2.34 ± 1.95 mg/dL. These values were increased by ≥25% in 5 patients. The mean sCr levels when symptoms resolved was 1.54 ± 0.67 mg/dL, and no significant difference was seen before, during, or after disease onset (P = 0.069). Two patients were diagnosed with HAdV viremia and 1 with acute tubulointerstitial nephritis revealed on biopsy. In addition to a reduction in immunosuppressant dosage, 2 patients received gammaglobulins and 5 received ganciclovir. CONCLUSION: Symptoms of all patients were alleviated, although some patients developed nephritis or viremia. Hence, the possibility of exacerbation should always be considered. Adequate follow-up observation should be conducted, and diligent and aggressive therapeutic intervention is required to prevent the condition from worsening.
  • 新規分子標的薬を用いた、原発性十二指腸癌に対する新規治療開発
    芝 聡美, 三木 厚, 大澤 英之, 寺谷 工, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 116回 OP - 5 2016/04
  • 消化器癌手術症例に対する術前栄養評価と術後合併症の関連
    倉科 憲太郎, 三木 厚, 松本 健司, 村越 美穂, 佐藤 敏子, 小暮 亜弥子, 齋藤 心, 鯉沼 広治, 笹沼 英紀, 小泉 大, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 116回 PS - 3 2016/04
  • 倉科 憲太郎, 細谷 好則, 齋藤 心, 松本 志郎, 小暮 亜弥子, 佐藤 敏子, 村越 美穂, 荒川 由起子, 岡田 憲樹, 春田 英律, 宇井 崇, 三木 厚, 堀江 久永, 佐久間 康成, 佐田 尚宏
    日本胃癌学会総会記事 (一社)日本胃癌学会 88回 344 - 344 2016/03 [Not refereed][Not invited]
  • 胃癌手術における周術期栄養剤投与による体重減少への影響 ランダム化比較試験
    倉科 憲太郎, 細谷 好則, 小暮 亜弥子, 村越 美穂, 佐藤 敏子, 荒川 由起子, 齋藤 心, 松本 志郎, 春田 英律, 宇井 崇, 三木 厚, 佐久間 康成, 堀江 久永, 佐田 尚宏
    日本静脈経腸栄養学会雑誌 (株)ジェフコーポレーション 31 (1) 326 - 326 2189-0161 2016/01
  • 三木 厚, 佐久間 康成, 大澤 英之, 芝 聡美, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐田 尚宏
    胆膵の病態生理 日本胆膵病態・生理研究会 32 (1) 35 - 37 2185-4564 2016 [Not refereed][Not invited]
     
    膵切除術を行った浸潤性膵管癌74例(男性46例、女性28例、平均66.6歳)を対象に、標記について検討した。膵体尾部癌は31例、膵頭部癌は43例で、臨床病期はStage Iが2例、Stage IIが8例、Stage IIIが40例、Stage IVaが24例であった。術後補助化学療法は50例に施行した。R0群の3年生存率は60.1%、R1群では41.0%であったが有意差は認められなかった。癌組織型では有意差はなく、T因子についてはT1〜T2群に対しT3〜T4群は有意に予後良好であった。リンパ節転移なし群に対し、あり群は有意に予後不良であった。多変量解析では、リンパ節転移の有無のみが独立した予後規定因子であった。リンパ節転移の有無(N)と癌遺残度(R)の検討では、R0N-群の3年生存率は85.8%、R0N+群では40.3%、R1N-群は50.0%、R1N+群は41.7%と、R0N-群で有意に予後良好であった。
  • 三木 厚, 佐久間 康成, 遠藤 和洋, 小泉 大, 笹沼 秀紀, 佐田 尚宏
    胆道 日本胆道学会 29 (3) 553 - 553 0914-0077 2015/08
  • 小切開創より膵離断を行った腹腔鏡補助下膵体尾部切除術2例の検討
    吉田 淳, 佐久間 康成, 森嶋 計, 笠原 尚哉, 三木 厚, 笹沼 英紀, 佐田 尚宏, 安田 是和
    小切開・鏡視外科学会雑誌 (NPO)小切開・鏡視外科学会 6 (1) 45 - 50 2185-2820 2015/08 [Not refereed][Not invited]
     
    当院では膵良性疾患に対して腹腔鏡下膵体尾部切除術(LDP)を施行しているが、視野確保が不十分な症例や高度癒着例、および膵切離面の状態からステイプラーによる切離閉鎖が困難な症例には、用手的補助下腹腔鏡下手術(HALS)や小切開手術(MIS)への移行が有用な場合がある。今回、LDPからHALSへ移行し、さらにMISへ移行することで確実な膵切離が行えた2例(61歳女、21歳女)を報告した。
  • 【慢性膵炎・急性膵炎に対する外科的アプローチ】 急性膵炎 急性膵炎の被包化壊死(WON)に対する外科的アプローチ
    佐田 尚宏, 鈴木 正徳, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成
    手術 金原出版(株) 69 (9) 1339 - 1344 0037-4423 2015/08 [Not refereed][Not invited]
  • 短腸症モデルラットを用いたポリアミン摂取による治療効果の検討と小腸再生促進メカニズムの解明
    笠原 尚哉, 寺谷 工, 三木 厚, 佐久間 康成, 藤本 康弘, 上本 伸二, 佐田 尚宏, 安田 是和
    日本消化器外科学会総会 (一社)日本消化器外科学会 70回 P - 8 2015/07
  • Resectability向上を目指した術前固有肝動脈塞栓療法
    三木 厚, 佐久間 康成, 森嶋 計, 笹沼 秀紀, 佐田 尚宏, 安田 是和
    日本肝胆膵外科学会・学術集会プログラム・抄録集 (一社)日本肝胆膵外科学会 27回 545 - 545 2015/06
  • 清水 敦, 遠藤 和洋, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 鈴木 正徳, 佐田 尚宏
    肝・胆・膵 (株)アークメディア 70 (6) 901 - 906 0389-4991 2015/06 [Not refereed][Not invited]
  • 肝胆膵 胆道閉鎖症における生体肝移植後拒絶反応の危険因子の解析 マイクロキメリズムの免疫寛容に対する影響
    眞田 幸弘, 川野 陽一, 三木 厚, 相田 順子, 仲村 賢一, 下村 七生貴, 石川 直, 新井 冨生, 平田 雄大, 山田 直也, 岡田 憲樹, 井原 欣幸, 浦橋 泰然, 安田 是和, 田久保 海誉, 水田 耕一
    日本外科学会定期学術集会抄録集 (一社)日本外科学会 115回 OP - 5 2015/04
  • 短腸症モデルラットを用いたポリアミン摂取による治療効果の検討
    笠原 尚哉, 寺谷 工, 三木 厚, 佐久間 康成, 藤本 康弘, 上本 伸二, 佐田 尚宏, 安田 是和
    日本消化器病学会雑誌 (一財)日本消化器病学会 112 (臨増総会) A394 - A394 0446-6586 2015/03
  • Atsushi Miki, Yasunaru Sakuma, Hideyuki Ohzawa, Yukihiro Sanada, Hideki Sasanuma, Alan T Lefor, Naohiro Sata, Yoshikazu Yasuda
    International surgery 100 (3) 480 - 5 0020-8868 2015/03 [Refereed][Not invited]
     
    We report a rare case of immunoglobulin G4 (IgG4)-related sclerosing cholangitis without other organ involvement. A 69-year-old-man was referred for the evaluation of jaundice. Computed tomography revealed thickening of the bile duct wall, compressing the right portal vein. Endoscopic retrograde cholangiopancreatography showed a lesion extending from the proximal confluence of the common bile duct to the left and right hepatic ducts. Intraductal ultrasonography showed a bile duct mass invading the portal vein. Hilar bile duct cancer was initially diagnosed and percutaneous transhepatic portal vein embolization was performed, preceding a planned right hepatectomy. Strictures persisted despite steroid therapy. Therefore, partial resection of the common bile duct following choledochojejunostomy was performed. Histologic examination showed diffuse and severe lymphoplasmacytic infiltration, and abundant plasma cells, which stained positive for anti-IgG4 antibody. The final diagnosis was IgG4 sclerosing cholangitis. Types 3 and 4 IgG4 sclerosing cholangitis remains a challenge to differentiate from cholangiocarcinoma. A histopathologic diagnosis obtained with a less invasive approach avoided unnecessary hepatectomy.
  • Hideyuki Ohzawa, Atsushi Miki, Yasuo Hozumi, Chieko Miyazaki, Yuka Sagara, Yumiko Tanaka, Satomi Shiba, Hiromi Joutoku, Masako Sakuragi, Megumi Takehara, Yasunaru Sakuma, Wataru Nishimura, Hirofumi Fujii, Yoshikazu Yasuda
    Oncology letters 9 (1) 119 - 124 1792-1074 2015/01 
    Chemotherapy-induced nausea and vomiting is a serious adverse side-effect of anthracycline-based chemotherapy regimens, in patients with breast cancer. A combination of three drugs, 5-hydroxytryptamine (5-HT3) receptor antagonist, aprepitant and dexamethasone, is recommended for antiemetic therapy. Palonosetron (PALO), a novel 5-HT3 receptor antagonist has been identified to be effective against delayed nausea and vomiting. In this study, the results of PALO for patients who received anthracycline-based chemotherapy were compared with that of granisetron (GRA) using a crossover study design. This study evaluated the efficacy of antiemetics in the first cycle of chemotherapy, as well as the second and third cycles. A total of 21 patients and 19 patients were assigned to PALO and GRA treatment groups during the first cycle of chemotherapy, respectively. The patients switched to the other antiemetic drug for the second chemotherapy cycle (PALO followed by GRA or GRA followed by PALO). The patients could select PALO or GRA antiemetics for the third cycle, according to their preference. A total of 21 patients selected PALO and 18 patients selected GRA in the third cycle, and one patient was withdrawn from the study as their third cycle questionnaire was not obtained. No significant differences between PALO and GRA were identified in first and second cycles. However, during the third cycle, a significant difference was observed in acute-phase complete control of emetic events between the PALO and GRA groups, which was defined as no emetic episode, no additional antiemetic treatment and no more than mild nausea, between PALO and GRA. These results demonstrated that changing antiemetics may affect the efficacy of antiemetics. This study indicates that alteration of antiemetic regimens, including drug combination and order, may improve the efficacy of antiemetic treatment.
  • Atsushi Miki, Camillo Ricordi, Toshiyuki Yamamoto, Yasunaru Sakuma, Ryosuke Misawa, Atsuyoshi Mita, Luca Inverardi, Rodolfo Alejandro, Hirohito Ichii
    Pancreas 43 (8) 1317 - 22 2014/11 
    OBJECTIVES: The effects of glucocorticoid during culture on human islet cells have been controversial. Exendin-4 (EX) enhances the insulin secretion and significantly improves clinical outcomes in islet cell transplantation. In this study, we examined the effects of glucocorticoids and EX on human islet cells during pretransplant culture. METHODS: Methylprednisolone (MP) and/or EX were added to the standard culture medium for clinical islet cell transplantation. Islets were cultured for 24 hours with 3 different conditions (control, no additives; MP alone; and MP + EX). β-Cell fractional viability, cellular composition, multiple cytokine/chemokine production, multiple phosphorylation proteins, and glucose-induced insulin secretion were evaluated. RESULTS: Viable β-cell survival in MP and MP + EX group was significantly higher than in the control group. Exendin-4 prevented MP-induced reduction of insulin secretion. Methylprednisolone supplementation to the culture medium decreased cytokine and chemokine production. Moreover, extracellular signal-regulated kinase 1/2 phosphorylation was significantly increased by MP and MP + EX. CONCLUSIONS: Glucocorticoid supplementation into culture media significantly decreased the cytokine/chemokine production and increased the extracellular signal-regulated kinase 1/2 phosphorylation, resulting in the improvement of human β-cell survival. In addition, EX maintained the insulin secretion suppressed by MP. The supplementation of MP and EX together could be a useful strategy to create suitable human islets for transplantation.
  • 当科における肝細胞癌初回切除症例116例の予後についての検討
    石黒 保直, 佐久間 康成, 笹沼 英紀, 藤原 岳人, 黒河内 顕, 三木 厚, 兼田 裕司, 森嶋 計, 佐田 尚宏, 安田 是和
    日本消化器外科学会雑誌 (一社)日本消化器外科学会 47 (Suppl.2) 211 - 211 0386-9768 2014/10
  • インスリノーマの局在診断における造影早期相thin slice CT撮影の有用性
    石岡 秀基, 石黒 保直, 森嶋 計, 三木 厚, 兼田 裕司, 黒河内 顕, 笹沼 英紀, 佐久間 康成, 佐田 尚宏, 安田 是和
    日本消化器外科学会雑誌 (一社)日本消化器外科学会 47 (Suppl.2) 258 - 258 0386-9768 2014/10
  • 【膵炎大全〜もう膵炎なんて怖くない〜】 膵炎各論 アルコール性膵炎
    佐田 尚宏, 田口 昌延, 笠原 直哉, 森嶋 計, 兼田 裕司, 三木 厚, 石黒 保直, 黒河内 顕, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 清水 敦, 栗原 克己, 安田 是和
    胆と膵 医学図書出版(株) 35 (臨増特大) 1125 - 1129 0388-9408 2014/10 [Not refereed][Not invited]
     
    アルコール摂取(多飲)は急性膵炎、慢性膵炎の成因として最も頻度が高い。他の成因と異なり、アルコール性膵炎は断酒・禁煙で発症・進展の予防が可能で、生活指導が重要な疾患である。その一方で「アルコール性」を規定するエタノール摂取量の明確な基準はなく、その発症・進展の機序についても不明な点が多い。慢性膵炎臨床診断基準2009では慢性膵炎をアルコール性と非アルコール性に分類し、発症前、発症早期に治療介入ができるように慢性膵炎疑診、早期慢性膵炎の診断基準を設定した。急性膵炎でも高リスク群であるアルコール多飲者の実際の発症率は低率で、遺伝子異常(SPINK1、PRSS1など)など、他の要因が深く関与していることが想定されている。アルコール性急性膵炎、慢性膵炎の診断・治療は、他の成因によるものと大きく変わるところはなく、介入治療は改訂アトランタ分類2012による膵炎局所合併症の新分類に応じた適切な時期・手技で行うことが推奨される。(著者抄録)
  • Yukihiro Sanada, Naoya Yamada, Masanobu Taguchi, Kazue Morishima, Naoya Kasahara, Yuji Kaneda, Atsushi Miki, Yasunao Ishiguro, Akira Kurogochi, Kazuhiro Endo, Masaru Koizumi, Hideki Sasanuma, Takehito Fujiwara, Yasunaru Sakuma, Atsushi Shimizu, Masanobu Hyodo, Naohiro Sata, Yoshikazu Yasuda
    INTERNATIONAL SURGERY 99 (4) 426 - 431 0020-8868 2014/07 [Refereed][Not invited]
     
    We report a 71-year-old man who had undergone pylorus-preserving pancreatoduodenectomy (PPPD) using PPPD-IV reconstruction for cholangiocarcinoma. For 6 years thereafter, he had suffered recurrent cholangitis, and also a right liver abscess (S5/8), which required percutaneous drainage at 9 years after PPPD. At 16 years after PPPD, he had been admitted to the other hospital because of acute purulent cholangitis. Although medical treatment resolved the cholangitis, the patient was referred to our hospital because of dilatation of the intrahepatic biliary duct (B2). Peroral double-balloon enteroscopy revealed that the diameter of the hepaticojejunostomy anastomosis was 12 mm, and cholangiography detected intrahepatic stones. Lithotripsy was performed using a basket catheter. At 1 year after lithotripsy procedure, the patient is doing well. Hepatobiliary scintigraphy at 60 minutes after intravenous injection demonstrated that deposit of the tracer still remained in the upper afferent loop jejunum. Therefore, we considered that the recurrent cholangitis, liver abscess, and intrahepatic lithiasis have been caused by biliary stasis due to nonobstructive afferent loop syndrome. Biliary retention due to nonobstructive afferent loop syndrome may cause recurrent cholangitis or liver abscess after hepaticojejunostomy, and double-balloon enteroscopy and hepatobiliary scintigraphy are useful for the diagnosis of nonobstructive afferent loop syndrome.
  • 清水 敦, 佐田 尚宏, 森嶋 計, 兼田 裕司, 石黒 保直, 三木 厚, 黒河内 顕, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 田中 昌宏, 安田 是和
    外科 (株)南江堂 76 (7) 747 - 752 0016-593X 2014/07 [Not refereed][Not invited]
     
    胆道手術におけるドレーン挿入の功罪を臨床試験の結果から検討した.膿瘍・穿孔などの合併症がない胆嚢摘出術では,開腹・腹腔鏡下ともにドレーンの挿入は創感染,呼吸器感染といった術後の合併症を増やし,それに見合う利点がないとされる.胆道切開や胆管切除・吻合でも同様に,ドレーンの利点が乏しいという結果が得られている.ルーチンワークとして行われがちなドレーンの挿入であるが,evidence-based medicine(EBM)に基づき再考すべきである.(著者抄録)
  • Yukihiro Sanada, Youichi Kawano, Atsushi Miki, Junko Aida, Ken-ichi Nakamura, Naotaka Shimomura, Naoshi Ishikawa, Tomio Arai, Yuta Hirata, Naoya Yamada, Noriki Okada, Taiichi Wakiya, Yoshiyuki Ihara, Taizen Urahashi, Yoshikazu Yasuda, Kaiyo Takubo, Koichi Mizuta
    Transplant International 27 (4) 383 - 390 0934-0874 2014/04
  • Ai Sadatomo, Koji Koinuma, Atsushi Miki, Hisanaga Horie, Yoshikazu Yasuda
    Gan to kagaku ryoho. Cancer & chemotherapy 40 (7) 943 - 5 0385-0684 2013/07 
    A 64-year-old man received mFOLFOX6+bevacizumab chemotherapy for metastatic lung cancer after rectal cancer resection( Stage IV). After 28 courses, he had an abdominal pain with fever, and computed tomography showed pelvic abscess with stercolith of appendix. He was diagnosed as acute appendicitis with intra-abdominal abscess, and emergency appendectomy with drainage was performed. Two days after the operation, he was suspected to have a sutural leakage as was suggested from the properties of his drainage, therefore re-operation was performed. A small hole of the ileum, about 2mm in diameter, was observed. The margin of the hole showed neither inflammatory nor neoplastic change, and a suturing closure of the hole was performed. The post-operative course was uneventful. Histopathological findings of the resected appendix suggested that the perforation was caused by necrosis of metastatic cancer cells penetrating the appendiceal wall. This is a case of a bevacizumab-related metachronous perforation that occurred in different gastrointestinal origins within a very short term.
  • 術後5年目に血行性前立腺転移を来たし、CK7+、20+パターンを呈したS状結腸癌の一例
    芝 順太郎, 齋藤 心, 三木 厚, 黒河内 顕, 太白 健一, 齋藤 晶, 宮倉 安幸, 堀江 久永, 安田 是和, 栗原 克己
    日本臨床外科学会雑誌 日本臨床外科学会 73 (増刊) 675 - 675 1345-2843 2012/10
  • Atsushi Miki, Yasuyuki Miyakura, Yasunaru Sakuma, Hidetoshi Kumano, Hisanaga Horie, Naohiro Sata, Alan T Lefor, Yoshikazu Yasuda
    The American surgeon 78 (9) E425-6  2012/09
  • Masaru Koizumi, Naohiro Sata, Masanobu Taguchi, Naoya Kasahara, Kazue Morishima, Yuji Kaneda, Atsushi Miki, Kunihiko Shimura, Hideki Sasanuma, Takehito Fujiwara, Makoto Ota, Atsushi Shimizu, Masanobu Hyodo, Alan T. Lefor, Yoshikazu Yasuda
    JOURNAL OF SURGICAL EDUCATION 69 (5) 605 - 610 1931-7204 2012/09 [Refereed][Not invited]
     
    OBJECTIVE: The Lichtenstein inguinal hernia repair is commonly performed and suitable for teaching basic surgical skills. The objective of this study is to evaluate the feasibility of this procedure for surgical training, particularly in regard to patient outcomes. DESIGN: Retrospective case review after introduction of an integrated teaching program. SETTING: University teaching hospital. PARTICIPANTS: The Lichtenstein inguinal hernia repair is the standard procedure for adult primary unilateral inguinal hernia since 2003 at Jichi Medical University. We introduced an integrated teaching system of lectures, skill training, and videos to teach the skills for Lichtenstein inguinal hernia repair to residents and junior faculty in 2003. Cases were retrospectively divided into 4 groups. based on the experience of the operating surgeon; junior residents (PGY 1-2, group A), senior residents (PGY 3-5, group B), junior faculty (PGY 6-10, group C), and senior faculty (PGY 11 or more, group D). Background, perioperative factors, and outcomes were evaluated among the groups. RESULTS: A total of 246 elective inguinal hernia repairs (group A: 136, group B: 49, group C: 42, group D: 19) were performed. There was a significant difference in the frequeney of concomitant diseases (p = 0.012) and anticoagulant therapy (p = 0.031). Average operating time was 80.7 +/- 24.9, 72.6 +/- 20.8, 63.5 +/- 22.0, and 54.7 +/- 27.9 (min +/- SD) in groups A, B, C, and D, respectively, with a significant difference between groups A and D (p < 0.001). No significant differences were observed in estimated blood loss (p = 0.216) or morbidity (p = 0.294). CONCLUSIONS: The Lichtenstein inguinal hernia repair can be safely performed by residents and junior faculty with the appropriate supervision of senior faculty without any disadvantage to patients. This integrated teaching program for Lichtenstein inguinal hernia repair is effective and feasible for training residents and junior faculty. (J Surg 69:605-610. (C) 2012 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.)
  • Hidenori Takahashi, Phillip Ruiz, Camillo Ricordi, Victor Delacruz, Atsushi Miki, Atsuyoshi Mita, Ryosuke Misawa, Scott Barker, George W Burke, Andreas G Tzakis, Hirohito Ichii
    Cell transplantation 21 (1) 113 - 25 2012 
    There is abundant evidence that immune cells infiltrating into a transplanted organ play a critical role for destructive inflammatory or regulatory immune reactions. Quantitative in situ analysis (i.e., in tissue sections) of immune cells remains challenging due to a lack of objective methodology. Laser scanning cytometry (LSC) is an imaging-based methodology that performs quantitative measurements on fluorescently and/ or chromatically stained tissue or cellular specimens at a single-cell level. In this study, we have developed a novel objective method for analysis of immune cells, including Foxp3(+) T regulatory cells (Tregs), on formalin-fixed /paraffin-embedded (FFPE) transplant biopsy sections using iCys® Research Imaging Cytometer. The development of multiple immunofluorescent staining was established using FFPE human tonsil sample. The CD4/CD8 ratio and the population of Tregs among CD4(+) cells were analyzed using iCys and compared with the results from conventional flow cytometry analysis (FCM). Our multiple immunofluorescent staining techniques allow obtaining clear staining on FFPE sections. The CD4/CD8 ratio analyzed by iCys was concordant with those obtained by FCM. This method was also applicable for liver, small intestine, kidney, pancreas, and heart transplant biopsy sections and provide an objective quantification of Tregs within the grafts.
  • 手術・そこが知りたい!(肝亜区域の同定と切離) 肝亜区域S2の同定と切離について 当科における小児生体肝移植ドナー手術での工夫
    藤原 岳人, 兼田 裕司, 森嶋 計, 三木 厚, 俵藤 正信, 太田 真, 清水 敦, 佐久間 康成, 眞田 幸弘, 脇屋 太一, 浦橋 泰然, 水田 耕一, 安田 是和
    日本臨床外科学会雑誌 日本臨床外科学会 72 (増刊) 408 - 408 1345-2843 2011/10
  • 腹膜播種を来たした胆管嚢胞腺癌の一例
    太田 真, 俵藤 正信, 藤原 岳人, 小泉 大, 三木 厚, 兼田 裕司, 黒河内 顕, 長瀬 通隆, 佐田 尚弘, 安田 是和
    日本肝胆膵外科学会・学術集会プログラム・抄録集 (一社)日本肝胆膵外科学会 23回 357 - 357 2011/06
  • Toshiyuki Yamamoto, Atsuyoshi Mita, Camillo Ricordi, Shari Messinger, Atsushi Miki, Yasunaru Sakuma, Francesca Timoneri, Scott Barker, Alessia Fornoni, R Damaris Molano, Luca Inverardi, Antonello Pileggi, Hirohito Ichii
    Transplantation 89 (11) 1328 - 35 2010/06 
    OBJECTIVES: Recent studies demonstrated that prolactin (PRL) has beneficial effects on beta cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. MATERIALS AND METHODS: Each human islet isolated from 14 deceased multiorgan donors was cultured in Miami modified media-1 supplemented with or without rhPRL (500 microg/L) for 48 hr. beta-Cell survival and proliferation (BrdU and Ki-67) were determined by laser scanning cytometry. The cytoprotective effects of rhPRL against noxious stimuli were assessed by flow cytometry (tetramethylrhodamine ethyl ester). Cytokine/chemokine and tissue factor productions were measured in vitro, and islet potency was assessed in vivo in diabetic immunodeficient mice. RESULTS: beta-Cell survival during culture was 37% higher in the rhPRL group than in control (P=0.029). rhPRL protected beta cells in vitro from cytokines, Nitric oxide donor, and H2O2. The exposure to rhPRL did not affect human beta-cell proliferation with our protocol. rhPRL treatment did not alter cytokine/chemokine and tissue factor production in vitro or affected human islet functionality in vivo: recipient mice achieved normoglycemia with a comparable tempo, whereas loss of graft function was observed in two of the seven mice in the control group and in none of the rhPRL group (p=n.s.). CONCLUSION: rhPRL supplementation to islet culture media improved human beta-cell-specific survival without altering islet quality. Addition of rhPRL to cultured islets may grant a more viable beta-cell mass in culture. The development of beta-cell cytoprotective strategies will be of assistance in improving islet transplantation outcomes.
  • Hirohito Ichii, Atsushi Miki, Toshiyuki Yamamoto, Ruth D Molano, Scott Barker, Atsuyoshi Mita, Rayner Rodriguez-Diaz, Dagmar Klein, Ricardo Pastori, Rodolfo Alejandro, Luca Inverardi, Antonello Pileggi, Camillo Ricordi
    Laboratory investigation; a journal of technical methods and pathology 88 (11) 1167 - 77 2008/11 
    Substantial amounts of nonendocrine cells are implanted as part of human islet grafts, and a possible influence of nonendocrine cells on clinical islet transplantation outcome has been postulated. There are currently no product release criteria specific for nonendocrine cells due to lack of available methods. The aims of this study were to develop a method for the evaluation of pancreatic ductal cells (PDCs) for clinical islet transplantation and to characterize them regarding phenotype, viability, and function. We assessed 161 human islet preparations using laser scanning cytometry (LSC/iCys) for phenotypic analysis of nonendocrine cells and flow cytometry (FACS) for PDC viability. PDC and beta-cells obtained from different density fractions during the islet cell purification were compared in terms of viability. Furthermore, we examined PDC ability to produce proinflammatory cytokines/chemokines, vascular endothelial growth factor (VEGF) and tissue factor (TF) relevant to islet graft outcome. Phenotypic analysis by LSC/iCys indicated that single staining for CK19 or CA19-9 was not enough for identifying PDCs, and that double staining for amylase and CK19 or CA19-9 allowed for quantitative evaluation of acinar cells and PDC content in human islet preparation. PDC showed a significantly higher viability than beta-cells (PDC vs beta-cell: 75.5+/-13.9 and 62.7+/-18.7%; P<0.0001). Although beta-cell viability was independent of its density, that of PDCs was higher as the density from which they were recovered increased. There was no correlation between PDCs and beta-cell viability (R(2)=0.0078). PDCs sorted from high-density fractions produced significantly higher amounts of proinflammatory mediators and VEGF, but not TF. We conclude that PDCs isolated from different fractions had different viability and functions. The precise characterization and assessment of these cells in addition to beta-cells in human islet cell products may be of assistance in understanding their contribution to islet engraftment and in developing strategies to enhance islet graft function.
  • Atsuyoshi Mita, Camillo Ricordi, Atsushi Miki, Scott Barker, Ross Haertter, Yasuhiko Hashikura, Shin-Ichi Miyagawa, George W Burke 3rd, Luca Inverardi, Hirohito Ichii
    Transplantation 86 (1) 46 - 53 2008/07 
    BACKGROUND: Sirolimus plays a critical role in facilitating steroid-free immunosuppression, in conjunction with low dose tacrolimus, in current islet transplantation. Although several studies have investigated the effects of sirolimus on islet cells, conflicting results have been reported. In this study, we assessed the effects of sirolimus supplementation in culture media on human islet preparations, focusing on the anti-proinflammatory aspects. METHODS: Human islet preparations were divided into four groups: pure (purity >90%) sirolimus (30 ng/mL); pure control (0 ng/mL); impure (purity 40%-60%) sirolimus; and impure control. All groups were cultured for 3 days and assessed regarding glucose stimulated insulin release, fractional beta-cell viability, beta-cell, and macrophage content. Cytokine and chemokine production from islet preparations and sorted pancreatic ductal cells were also examined. RESULTS: Stimulated insulin release in the impure sirolimus group was significantly increased (P=0.024), as previously reported. Although fractional beta-cell viability showed no significant differences, beta-cell survival during culture significantly increased in impure sirolimus group when compared with the impure control group (P=0.015). Tumor necrosis factor-alpha, interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1beta production from the impure sirolimus group significantly decreased (P<0.05). Furthermore, tumor necrosis factor-alpha and macrophage inflammatory protein-1beta production from sorted ductal cells significantly decreased in the sirolimus group (P<0.05). The number of macrophages contained in islet preparations significantly decreased in the impure sirolimus group when compared with the impure control group (P<0.05). CONCLUSIONS: Sirolimus improved not only stimulated insulin release, but also beta-cell survival during culture. The antiinflammatory effects of sirolimus also appear beneficial to islet cells in culture and may be a useful strategy in improving islet transplantation outcomes.
  • Toshiyuki Yamamoto, Camillo Ricordi, Shari Messinger, Yasunaru Sakuma, Atsushi Miki, Rayner Rodriguez, Ross Haertter, Aisha Khan, Rodolfo Alejandro, Hirohito Ichii
    Transplantation 84 (8) 997 - 1002 0041-1337 2007/10 
    BACKGROUND: The quality and stability of enzyme blends used in islet cell processing are critical for successful human islet isolation. A wide variability in enzymatic activity among lots of Liberase HI has been reported. This study examines the interlot and intralot variability of Liberase HI and the over-time deterioration of enzyme quality based on the analysis of islet isolation outcomes. METHODS: The data of 169 human isolations processed for clinical islet transplantation, using five different lots of Liberase HI, were retrospectively analyzed. Inter- and intralot variables in the islet isolation were assessed over a 15-month period. RESULTS: The analysis revealed significant interlot differences in the digestion time, prepurification islet counts, percent recovery, viability, and glucose stimulation insulin index. Moreover, a significant decrease in the pre- and postpurification islet yield per pancreas weight (IEQ/g) in isolations processed by two different enzyme lots used over a 15-month period was observed, suggesting a progressive deterioration of enzyme quality. CONCLUSIONS: Our data demonstrate a significant lot-to-lot related variability in islet isolation outcomes. In addition, the over-time decline in isolation outcomes processed using a single enzyme lot was observed even when the enzyme blends were used within the expiration dating specified by the manufacturer.
  • Jorge D Rivas-Carrillo, Alejandro Soto-Gutierrez, Nalu Navarro-Alvarez, Hirofumi Noguchi, Teru Okitsu, Yong Chen, Takeshi Yuasa, Kimiaki Tanaka, Michiki Narushima, Atsushi Miki, Haruo Misawa, Yasuhiko Tabata, Hee-Sook Jun, Shinichi Matsumoto, Ira J Fox, Noriaki Tanaka, Naoya Kobayashi
    Diabetes 56 (5) 1259 - 67 2007/05 
    OBJECTIVE: Treatment of diabetic patients by pancreatic islet transplantation often requires the use of islets from two to four donors to produce insulin independence in a single recipient. Following isolation and transplantation, islets are susceptible to apoptosis, which limits their function and probably long-term islet graft survival. RESEARCH DESIGN AND METHODS: To address this issue, we examined the effect of the cell-permeable apoptosis inhibitor pentapeptide Val-Pro-Met-Leu-Lys, V5, on pancreatic islets in a mouse model. RESULTS: V5 treatment upregulated expression of anti-apoptotic proteins Bcl-2 and XIAP (X-linked inhibitor of apoptosis protein) by more than 3- and 11-fold and downregulated expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-kappaB-p65 by 10, 30, and nearly 50%, respectively. Treatment improved the recovered islet mass following collagenase digestion and isolation by 44% and in vitro glucose-responsive insulin secretion nearly fourfold. Following transplantation in streptozotocin-induced diabetic mice, 150 V5-treated islet equivalents functioned as well as 450 control untreated islet equivalents in normalizing blood glucose. CONCLUSIONS: These studies indicate that inhibition of apoptosis by V5 significantly improves islet function following isolation and improves islet graft function following transplantation. Use of this reagent in clinical islet transplantation could have a dramatic impact on the number of patients that might benefit from this therapy and could affect long-term graft survival.
  • Alejandro Soto-Gutiérrez, Naoya Kobayashi, Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Debiao Zhao, Teru Okitsu, Hirofumi Noguchi, Hesham Basma, Yashuhiko Tabata, Yong Chen, Kimiaki Tanaka, Michiki Narushima, Atsushi Miki, Tadayoshi Ueda, Hee-Sook Jun, Ji-Won Yoon, Jane Lebkowski, Noriaki Tanaka, Ira J Fox
    Nature biotechnology 24 (11) 1412 - 9 1087-0156 2006/11 
    Severe acute liver failure, even when transient, must be treated by transplantation and lifelong immune suppression. Treatment could be improved by bioartificial liver (BAL) support, but this approach is hindered by a shortage of human hepatocytes. To generate an alternative source of cells for BAL support, we differentiated mouse embryonic stem (ES) cells into hepatocytes by coculture with a combination of human liver nonparenchymal cell lines and fibroblast growth factor-2, human activin-A and hepatocyte growth factor. Functional hepatocytes were isolated using albumin promoter-based cell sorting. ES cell-derived hepatocytes expressed liver-specific genes, secreted albumin and metabolized ammonia, lidocaine and diazepam. Treatment of 90% hepatectomized mice with a subcutaneously implanted BAL seeded with ES cell-derived hepatocytes or primary hepatocytes improved liver function and prolonged survival, whereas treatment with a BAL seeded with control cells did not. After functioning in the BAL, ES cell-derived hepatocytes developed characteristics nearly identical to those of primary hepatocytes.
  • Hideaki Ikeda, Naoya Kobayashi, Yoshihito Tanaka, Shuhei Nakaji, Chen Yong, Teru Okitsu, Mizuko Oshita, Shinichi Matsumoto, Hirofumi Noguchi, Michiki Narushima, Kimiaki Tanaka, Atsushi Miki, Jorge David Rivas-Carrillo, Alejandro Soto-Gutierrez, Nalu Navarro-Alvarez, Koichi Tanaka, Hee-Sook Jun, Noriaki Tanaka, Ji-Won Yoon
    Tissue engineering 12 (7) 1799 - 809 1076-3279 2006/07 
    Construction of a safe and functional bioartificial pancreas (BAP) that provides an adequate environment for islet cells may be an important approach to treating diabetic patients. Various types of BAP devices have been developed, but most of them involve extravascular implantation of islets in microcapsules or diffusion chambers. These devices have poor diffusive exchange between the islets and blood, and often rupture. To overcome these problems, we developed a new type of BAP composed of polyethylene-vinyl alcohol (EVAL) hollow fibers that are permeable to glucose and insulin and a poly-amino-urethane-coated, non-woven polytetrafluoroethylene (PTFE) fabric that allows cell adhesion. Porcine islets attached to the surface of the PTFE fabric, but not to the surface of the EVAL hollow fibers, allowing nutrient and oxygen exchange between blood flowing inside the fibers and cells outside. We inoculated this BAP with porcine islets and connected it to the circulation of totally pancreatectomized diabetic pigs. We found that blood glucose levels were reduced to a normal range and general health was improved, resulting in longer survival times. In addition, regulation of insulin secretion from the BAP was properly controlled in response to glucose both in vitro and in vivo. These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans.
  • Atsushi Miki, Michiki Narushima, Teru Okitsu, Yuichi Takeno, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Nalú Navarro-Alvarez, Yong Chen, Kimiaki Tanaka, Hirofumi Noguchi, Shinichi Matsumoto, Michinori Kohara, Jonathan R T Lakey, Eiji Kobayashi, Noriaki Tanaka, Naoya Kobayashi
    Cell transplantation 15 (4) 325 - 334 2006/04 
    Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.
  • Kimiaki Tanaka, Naoya Kobayashi, Alejandro Soto Gutierrez, Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Yong Chen, Michiki Narushima, Atsushi Miki, Teru Okitsu, Hirofumi Noguchi, Noriaki Tanaka
    Transplantation 81 (3) 427 - 37 0041-1337 2006/02 
    BACKGROUND: Because hepatocyte transplantation has been considered to be an attractive method to treat acute liver failure (ALF), efficient recovery of hepatocytes and maintenance of differentiated hepatocyte functions is of extreme importance. We here report the usefulness of an antiapoptotic pentapeptide V5, composed of Val-Pro-Met-Leu-Lys, in the monkey hepatocyte cultures. METHODS: We evaluated albumin production, metabolizing abilities of ammonia, lidocaine, and diazepam of monkey hepatocytes cultured with V5. The protein expression of apoptosis-associated molecules was analyzed using power blot analysis. An unwoven cloth inoculated with V5-treated monkey hepatocytes was transplanted on the surface of the spleen of both SCID mice and Balb/c mice suffering from ALF induced by 90% hepatectomy. RESULTS: When 100 microM V5 was utilized, ammonia-, lidocaine- and diazepam- metabolizing capacities and albumin production ability were significantly increased in V5-treated monkey hepatocytes. Such hepatocytes showed decreased Annexin V binding and increased the expression of anti-apoptotic and/or cytoprotective molecules, including Ku70, NF-kappaB, IKAP, hILP/XIAP, IkappaB, and CAS. Transplantation of the cloth containing the monkey hepatocytes significantly improved blood levels of glucose and ammonia and encephalopathy score and prolonged the survival of the mice with ALF. CONCLUSIONS: The present work clearly demonstrates the usefulness of V5 for maintaining the functions of monkey hepatocytes in tissue culture.
  • Atsushi Miki, Jorge D Rivas-Carrillo, Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, Yong Chen, Kimiaki Tanaka, Michiki Narushima, Yasuhiko Tabata, Teru Okitsu, Hirofumi Noguchi, Shinichi Matsumoto, Noriaki Tanaka, Naoya Kobayashi
    Cell transplantation 15 (10) 893 - 901 0963-6897 2006 
    Development of a subcutaneously implantable bioartificial pancreas (BAP) with immunoisolatory function could have a great impact on the treatment of diabetes mellitus. We have developed an implantable BAP device with an ethylene vinyl alcohol (EVAL) membrane. In the present study, we used basic fibroblast growth factors (bFGF), which was incorporated in a carrier for sustained release, in order to induce neovascularization when the device was implanted subcutaneously. To maintain the vasculature thus formed, a cell infusion port was attached to the BAP device, through which the device was filled with human liver vascular endothelial cell line TMNK-1, and the vasculature could be adequately maintained. Mice were divided into the following three groups. In group 1, a bFGF-free BAP device was implanted subcutaneously. In group 2, a sustained-release bFGF-impregnated BAP device was implanted. In group 3, a sustained-release bFGF-impregnated BAP device was implanted, and 3 x 10(6) TMNK-1 cells were infused into the implanted device every week. Neovascularization induced in the subcutaneous tissue around the implanted BAP device was macroscopically examined and histologically evaluated. In addition, the tissue blood flow was measured using a laser blood flow meter. In mice in group 3, neovascularization was significantly induced and maintained until week 8 postimplantation. It was confirmed by scanning electron microscopy that infused TMNK-1 cells adhered to the inner polyethylene surface of the device. It was demonstrated that the use of bFGF and vascular endothelial TMNK-1 cells induced and maintained adequate vasculature and tissue blood flow surrounding the implantable bag-type BAP device. We believe that the present study will contribute to BAP development for the treatment of diabetes.
  • Haruo Misawa, Naoya Kobayashi, Alejandro Soto-Gutierrez, Yong Chen, Aki Yoshida, Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Kimiaki Tanaka, Atsushi Miki, Jiro Takei, Tadayoshi Ueda, Masato Tanaka, Hirosuke Endo, Noriaki Tanaka, Toshifumi Ozaki
    Cell transplantation 15 (10) 903 - 10 0963-6897 2006 
    Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment for cell penetration and settlement. We therefore attempted to explore various materials that may allow the cells to penetrate and engraft in bone defects. PuraMatrix is a self-assembling peptide scaffold that produces a nanoscale environment allowing both cellular penetration and engraftment. The objective of this study was to investigate the effect of PuraMatrix on bone regeneration in a mouse bone defect model of the calvaria. Matrigel was used as a control. The expression of bone-related genes (alkaline phosphatase, Runx2, and Osterix) in the PuraMatrix-injected bone defects was stronger than that in the Matrigel-injected defects. Soft X-ray radiographs revealed that bony bridges were clearly observed in the defects treated with PuraMatrix, but not in the Matrigel-treated defects. Notably, PuraMatrix treatment induced mature bone tissue while showing cortical bone medullary cavities. The area of newly formed bones at the site of the bone defects was 1.38-fold larger for PuraMatrix than Matrigel. The strength of the regenerated bone was 1.72-fold higher for PuraMatrix (146.0 g) than for Matrigel (84.7 g). The present study demonstrated that PuraMatrix injection favorably induced functional bone regeneration.
  • Atsushi Miki, Michiki Narushima, Teru Okitsu, Yuichi Takeno, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Nalú Navarro-Alvarez, Yong Chen, Kimiaki Tanaka, Hirofumi Noguchi, Shinichi Matsumoto, Michinori Kohara, Jonathan R T Lakey, Eiji Kobayashi, Noriaki Tanaka, Naoya Kobayashi
    Cell transplantation 15 (4) 325 - 34 0963-6897 2006 
    Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for alpha-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.
  • Michiki Narushima, Naoya Kobayashi, Teru Okitsu, Yoshihito Tanaka, Shun-Ai Li, Yong Chen, Atsushi Miki, Kimiaki Tanaka, Shuhei Nakaji, Kohji Takei, Alejandro Soto Gutierrez, Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Hee-Sook Jun, Karen A Westerman, Hirofumi Noguchi, Jonathan R T Lakey, Philippe Leboulch, Noriaki Tanaka, Ji-Won Yoon
    Nature biotechnology 23 (10) 1274 - 82 1087-0156 2005/10 
    A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase (hTERT) cDNAs flanked by paired loxP recombination targets, which allow deletion of SV40T and TERT by Cre recombinase. Reverted NAKT-15 cells expressed beta-cell transcription factors (Isl-1, Pax 6, Nkx 6.1, Pdx-1), prohormone convertases 1/3 and 2, and secretory granule proteins, and secreted insulin in response to glucose, similar to normal human islets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks; mice remained normoglycemic for longer than 30 weeks. The establishment of this cell line is one step toward a potential cure of diabetes by transplantation.
  • Yong Chen, Naoya Kobayashi, Satoshi Suzuki, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Kimiaki Tanaka, Nalú Navarro-Alvarez, Takuya Fukazawa, Michiki Narushima, Atsushi Miki, Teru Okitsu, Hiroshi Amemiya, Noriaki Tanaka
    Transplantation 79 (10) 1378 - 85 0041-1337 2005/05 
    BACKGROUND: Considering the scarcity of donor livers, it is extremely important to establish a functional culture method for isolated hepatocytes. As a tool for maintaining hepatocyte functions in vitro, dHGF, a variant of HGF (hepatocyte growth factor) with a deletion of five amino acids, attracted our attention because it is less cytotoxic compared with HGF. METHODS: We evaluated growth, albumin production, metabolizing abilities of ammonia, lidocaine, and diazepam of human hepatocytes in the presence of dHGF (10-1000 ng/ml). The gene expression of liver markers was comparatively analyzed. The effect of intrasplenic transplantation of dHGF-treated human hepatocytes into severe combined immunodeficient (SCID) mice was evaluated in an acute liver failure (ALF) model induced by D-galactosamine (D-gal). RESULTS: When 100 ng/ml of dHGF was utilized, metabolism rates of ammonia, lidocaine, and diazepam and albumin production per unit cell significantly increased. The gene expression analysis demonstrated the enhanced expression of albumin, HNF-4alpha, and C/EBPalpha in the hepatocytes treated with 100 ng/ml of dHGF. Transplantation of such hepatocytes prolonged the survival of the SCID mice with ALF induced by D-gal. CONCLUSIONS: The present work clearly demonstrates the usefulness of dHGF (100 ng/ml) for maintaining the differentiated functions of human hepatocytes in tissue culture.

MISC

  • Yuichi Aoki, Hisashi Oshiro, Akihiko Yoshida, Kazue Morishima, Atsushi Miki, Hideki Sasanuma, Yasunaru Sakuma, Alan Kawarai Lefor, Naohiro Sata  BMC gastroenterology  20-  (1)  105  -105  2020/04  [Not refereed][Not invited]
     
    BACKGROUND: Capicua transcriptional repressor (CIC) -rearranged sarcoma is characterized by small round cells, histologically similar to Ewing sarcoma. However, CIC-rearranged sarcoma has different clinical, histological, and immunohistochemical features from Ewing sarcoma. It is important to differentiate between these tumors. CASE PRESENTATION: The patient is a 44-year-old man with a duodenal tumor diagnosed in another hospital who presented with a history of melena. Laboratory studies showed anemia with a serum hemoglobin of 6.0 g/dL. He was hospitalized and gastrointestinal bleeding was controlled successfully with endoscopy. However, he suffered from appetite loss and vomiting and progression of anemia a few weeks after presentation. Upper gastrointestinal endoscopy showed a circumferential soft tumor in the second portion of the duodenum and the endoscope could not pass distally. Computed tomography scan showed a greater than 10 cm tumor in the duodenum, with compression of the inferior vena cava and infiltrating the ascending colon. A definitive pathologic diagnosis could not be established despite four biopsies from the tumor edge. Due to gastrointestinal obstruction and progression of anemia, a pylorus-preserving pancreaticoduodenectomy with partial resection of the inferior vena cava and right hemicolectomy was performed as a complete tumor resection. The tumor was diagnosed as a CIC-rearranged sarcoma, but 2 months postoperatively local recurrence and distant metastases to the liver and lung were found. The patient died 3 months after surgery. CONCLUSIONS: Although the only definitive treatment for CIC-rearranged sarcoma is surgical resection, the CIC-rearranged sarcoma is highly malignant with a poor prognosis even after radical resection. More research is needed to establish optimal treatment strategies.
  • 吉田 淳, 笹沼 英紀, 片野 匠, 篠原 翔一, 森嶋 計, 三木 厚, 宮戸 英世, 遠藤 和洋, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  胆道  33-  (3)  581  -581  2019/10  [Not refereed][Not invited]
  • 篠原 翔一, 笹沼 英紀, 宮戸 英世, 森嶋 計, 三木 厚, 遠藤 和洋, 吉田 淳, 佐久間 康成, 北山 丈二, 佐田 尚宏  胆道  33-  (3)  628  -628  2019/10  [Not refereed][Not invited]
  • 窪木 大悟, 笹沼 英紀, 橋本 雄介, 三木 厚, 吉田 淳, 森嶋 計, 遠藤 和洋, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  胆道  33-  (3)  670  -670  2019/10  [Not refereed][Not invited]
  • リウマチに対してメトトレキサート内服中に発症した、肝原発悪性リンパ腫の一例
    大豆生田 尚彦, 森嶋 計, 宮戸 秀世, 遠藤 和洋, 三木 厚, 吉田 厚, 笹沼 英紀, 山口 博紀, 佐久間 康成, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏  日本臨床外科学会雑誌  80-  (増刊)  785  -785  2019/10  [Not refereed][Not invited]
  • 晩期合併症・トランジション 先天性胆道拡張症術後晩期合併症の検討
    吉田 淳, 笹沼 英紀, 宮戸 英世, 森嶋 計, 三木 厚, 兼田 裕司, 遠藤 和洋, 山口 博紀, 佐久間 康成, 川平 洋, 堀江 久永, 細谷 好則, 味村 俊樹, 北山 丈二, 佐田 尚宏  日本膵・胆管合流異常研究会プロシーディングス  42-  30  -30  2019/09  [Not refereed][Not invited]
  • 腎細胞癌膵転移切除症例の検討
    吉田 淳, 佐久間 康成, 森嶋 計, 三木 厚, 遠藤 和洋, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  74回-  P255  -6  2019/07  [Not refereed][Not invited]
  • 吉田 淳, 佐久間 康成, 森嶋 計, 遠藤 和洋, 三木 厚, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  膵臓  34-  (3)  A285  -A285  2019/06  [Not refereed][Not invited]
  • 青木 裕一, 笹沼 英紀, 森嶋 計, 三木 厚, 遠藤 和洋, 吉田 淳, 佐久間 康成, 佐田 尚宏  膵臓  34-  (3)  A379  -A379  2019/06  [Not refereed][Not invited]
  • 十二指腸乳頭部腫瘍に対する外科的乳頭切除術による段階的治療戦略
    小泉 大, 斎藤 晶, 青木 裕一, 森嶋 計, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 吉田 淳, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本外科学会定期学術集会抄録集  119回-  PS  -7  2019/04  [Not refereed][Not invited]
  • 12年の長期経過観察中に悪性化したと考えられる膵粘液嚢胞性腫瘍の一例
    高橋 礼, 笹沼 英紀, 三木 厚, 森嶋 計, 吉田 淳, 遠藤 和洋, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏, 池田 恵理子, 福島 敬宜  日本消化器病学会関東支部例会プログラム・抄録集  354回-  49  -49  2019/04  [Not refereed][Not invited]
  • 膵十二指腸動脈瘤の3例
    渡部 晃佑, 吉田 淳, 高橋 和也, 青木 裕一, 田口 昌延, 森嶋 計, 三木 厚, 兼田 裕司, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本腹部救急医学会雑誌  39-  (2)  345  -345  2019/02  [Not refereed][Not invited]
  • 鼠径部ヘルニア嵌頓症例における経過時間と腸切除の検討
    小泉 大, 斎藤 晶, 青木 裕一, 森嶋 計, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 吉田 淳, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本腹部救急医学会雑誌  39-  (2)  379  -379  2019/02  [Not refereed][Not invited]
  • Yuichi Aoki, Atsushi Miki, Tomoyuki Nakano, Hideki Sasanuma, Yasunaru Sakuma, Hisanaga Horie, Yoshinori Hosoya, Noriyoshi Fukushima, Alan Kawarai Lefor, Naohiro Sata  BMC cancer  18-  (1)  1249  -1249  2018/12  [Not refereed][Not invited]
     
    BACKGROUND: Thymomas are typically slow-growing tumors and AB type thymomas are considered no/low risk tumors with a better prognosis. Extra-thoracic metastases are extremely rare. To the best of our knowledge, no patient with an isolated splenic metastasis from a thymoma has been reported. We report a patient who underwent laparoscopic splenectomy for a slow-growing, isolated splenic metastasis, eight years after thymectomy. CASE PRESENTATION: The patient is a 78-year-old man. Eight years previously, the patient underwent extended thymectomy and postoperative radiation therapy for a thymoma. Five years after thymectomy, a nodule appeared in the spleen, and the lesion enlarged gradually for three years thereafter. The patient was referred for further examination and treatment. Computed tomography scan showed a sharply circumscribed 50 mm tumor slightly hypodense and heterogeneous lesion in the spleen. On T2-weighted images on Magnetic Resonance Imaging, the tumor had high intensity, equivalent to or slightly lower than that on T1-weighted images, and no decrease on diffusion-weighted images. The tumor was multinodular and showed a low-signal spoke-wheel sign in the margin, enhanced gradually in the dynamic study. Positron emission tomography-CT scan, showed relatively low accumulation. Surgical resection was undertaken, and pathological examination showed metastatic thymoma. The patient is without recurrence and has no other symptoms three years after splenectomy. CONCLUSIONS: This is the first report of an isolated splenic metastasis from a thymoma. Further cases are needed to standardize this surgery for such lesions.
  • 複雑な部分内臓逆位症を伴う総胆管結石症に対し手術を行った1例
    丸藤 琴音, 吉田 淳, 笹沼 英紀, 宮戸 秀世, 目黒 由行, 森嶋 計, 三木 厚, 遠藤 和洋, 小泉 大, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本臨床外科学会雑誌  79-  (増刊)  852  -852  2018/10  [Not refereed][Not invited]
  • 上部空腸憩室穿孔による急性汎発性腹膜炎の1例
    齋藤 俊祐, 利府 数馬, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 細谷 好則, 堀江 久永, 北山 丈二, 佐田 尚宏  日本臨床外科学会雑誌  79-  (3)  652  -653  2018/03  [Not refereed][Not invited]
  • 腹腔鏡下膵体尾部切除における電動式自動縫合器を使った膵離断定型化の試み
    笹沼 英紀, 佐久間 康成, 兼田 裕司, 三木 厚, 遠藤 和洋, 小泉 大, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本内視鏡外科学会雑誌  22-  (7)  EP141  -02  2017/12  [Not refereed][Not invited]
  • 腹腔鏡下膵体尾部切除における電動式自動縫合器を使った膵離断定型化の試み
    笹沼 英紀, 佐久間 康成, 兼田 裕司, 三木 厚, 遠藤 和洋, 小泉 大, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本内視鏡外科学会雑誌  22-  (7)  EP141  -02  2017/12  [Not refereed][Not invited]
  • 膵頭十二指腸切除での再建の工夫 Blumgart変法(自治医大方式)による膵空腸吻合の成績
    小泉 大, 斎藤 晶, 兼田 裕司, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本臨床外科学会雑誌  78-  (増刊)  425  -425  2017/10  [Not refereed][Not invited]
  • 十二指腸腫瘍性病変に対する膵温存十二指腸切除術の治療成績
    齋藤 晶, 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  PH17  -6  2017/07  [Not refereed][Not invited]
  • 切除可能膵癌に対する術前治療戦略の意義 切除可能膵癌に対する術前化学放射線治療の有用性
    三木 厚, 佐久間 康成, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  O2  -65  2017/07  [Not refereed][Not invited]
  • Blumgart-dumpling法(Blumgart変法 自治医大方式)による膵空腸吻合の工夫
    小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  RS2  -78  2017/07  [Not refereed][Not invited]
  • 胃切除後の胆道結石症の検討
    木村 有希, 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  PP2  -1  2017/07  [Not refereed][Not invited]
  • 当科における膵IPMNの縮小手術の検討
    齋藤 匠, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  PK4  -6  2017/07  [Not refereed][Not invited]
  • 退形成性膵管癌の6切除例
    風當 ゆりえ, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  PL4  -1  2017/07  [Not refereed][Not invited]
  • 切除可能膵癌に対する術前治療戦略の意義 切除可能膵癌に対する術前化学放射線治療の有用性
    三木 厚, 佐久間 康成, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  O2  -65  2017/07  [Not refereed][Not invited]
  • Blumgart-dumpling法(Blumgart変法 自治医大方式)による膵空腸吻合の工夫
    小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  RS2  -78  2017/07  [Not refereed][Not invited]
  • 十二指腸腫瘍性病変に対する膵温存十二指腸切除術の治療成績
    齋藤 晶, 小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本消化器外科学会総会  72回-  PH17  -6  2017/07  [Not refereed][Not invited]
  • 質感と人体解剖を忠実に再現した、高難度手術教育のための臓器モデル 鏡視下手術の教育効果を高めるために必要な技術トレーニング
    遠藤 和洋, 太白 健一, 笠原 尚哉, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本外科学会定期学術集会抄録集  117回-  SF  -48  2017/04  [Not refereed][Not invited]
  • 膵空腸吻合Blumgart変法(自治医大方式)に適した膵臓の検討
    小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, Lefor Alan, 佐田 尚宏  日本外科学会定期学術集会抄録集  117回-  PS  -134  2017/04  [Not refereed][Not invited]
  • IPMN国際診療ガイドラインからみた当院でのIPMN手術例の検討
    齋藤 匠, 三木 厚, 笠原 尚哉, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本外科学会定期学術集会抄録集  117回-  PS  -139  2017/04  [Not refereed][Not invited]
  • 家族性大腸腺腫症に合併した十二指腸病変の検討
    木村 有希, 小泉 大, 笠原 尚哉, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本外科学会定期学術集会抄録集  117回-  PS  -176  2017/04  [Not refereed][Not invited]
  • 膵体尾部を中心とした左側感染性WONに対する治療戦略
    笹沼 英紀, 佐久間 康成, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 堀江 久永, 細谷 好則, 佐田 尚宏  日本腹部救急医学会雑誌  37-  (2)  292  -292  2017/02  [Not refereed][Not invited]
  • 上腹部手術既往のある症例における腹腔鏡下肝切除の検討
    谷口 理丈, 佐久間 康成, 森嶋 計, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐田 尚宏  日本内視鏡外科学会雑誌  21-  (7)  OS4  -2  2016/12  [Not refereed][Not invited]
  • 医工連携による革新的内視鏡手術トレーニングシステムの開発 外科教育の新たな手法確立に向けて
    遠藤 和洋, 太白 健一, 笠原 直哉, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本内視鏡外科学会雑誌  21-  (7)  OS145  -6  2016/12  [Not refereed][Not invited]
  • 腹腔鏡下肝切除にRFAを併用して治療した肝細胞癌4例の経験
    森嶋 計, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏  日本内視鏡外科学会雑誌  21-  (7)  DP99  -1  2016/12  [Not refereed][Not invited]
  • Naoya Yamada, Yukihiro Sanada, Takumi Katano, Masahisa Tashiro, Yuta Hirata, Noriki Okada, Yoshiyuki Ihara, Atsushi Miki, Hideki Sasanuma, Taizen Urahashi, Yasunaru Sakuma, Koichi Mizuta  World journal of gastroenterology  22-  (44)  9865  -9870  2016/11  [Not refereed][Not invited]
     
    This is the first report of living donor liver transplantation (LDLT) for congenital hepatic fibrosis (CHF) using a mother's graft with von Meyenburg complex. A 6-year-old girl with CHF, who suffered from recurrent gastrointestinal bleeding, was referred to our hospital for liver transplantation. Her 38-year-old mother was investigated as a living donor and multiple biliary hamartoma were seen on her computed tomography and magnetic resonance imaging scan. The mother's liver function tests were normal and she did not have any organ abnormality, including polycystic kidney disease. LDLT using the left lateral segment (LLS) graft from the donor was performed. The donor LLS graft weighed 250 g; the graft recipient weight ratio was 1.19%. The operation and post-operative course of the donor were uneventful and she was discharged on post-operative day (POD) 8. The graft liver function was good, and the recipient was discharged on POD 31. LDLT using a graft with von Meyenburg complex is safe and useful. Long-term follow-up is needed with respect to graft liver function and screening malignant tumors.
  • 崎尾亮太郎, 三木厚, 森嶋計, 笠原尚哉, 笹沼英紀, 佐久間康成, 佐田尚宏, 安田是和  日本臨床外科学会雑誌  77-  (10)  2618  2016/10  [Not refereed][Not invited]
  • 小泉大, 木村有希, 笠原尚哉, 三木厚, 遠藤和洋, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 北山丈二, 佐田尚宏  日本臨床外科学会雑誌  77-  (増刊)  578  -578  2016/10  [Not refereed][Not invited]
  • 遠藤和洋, 太白健一, 笠原直哉, 三木厚, 小泉大, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 北山丈二, 佐田尚宏  日本臨床外科学会雑誌  77-  (増刊)  527  -527  2016/10  [Not refereed][Not invited]
  • 木村有希, 小泉大, 笠原尚哉, 三木厚, 遠藤和洋, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 北山丈二, 佐田尚宏  日本臨床外科学会雑誌  77-  (増刊)  405  -405  2016/10  [Not refereed][Not invited]
  • 齋藤晶, 森嶋計, 笠原尚哉, 三木厚, 笹沼英紀, 佐久間康成, 佐田尚宏, 安田是和  日本臨床外科学会雑誌  77-  (9)  2403  -2403  2016/09  [Not refereed][Not invited]
  • Yukihiro Sanada, Yasunaru Sakuma, Hideki Sasanuma, Atsushi Miki, Takumi Katano, Yuta Hirata, Noriki Okada, Naoya Yamada, Yoshiyuki Ihara, Taizen Urahashi, Naohiro Sata, Yoshikazu Yasuda, Koichi Mizuta  World journal of gastroenterology  22-  (34)  7851  -6  2016/09  [Not refereed][Not invited]
     
    Utilizing the opened round ligament as venous grafts during liver transplantation is useful but controversial, and there are no pathological analyses of this procedure. Herein, we describe the first reported case of a pathological analysis of an opened round ligament used as a venous patch graft in a living donor liver transplantation (LDLT). A 13-year-old female patient with biliary atresia underwent LDLT using a posterior segment graft from her mother. The graft had two hepatic veins (HVs), which included the right HV (RHV; 15 mm) and the inferior RHV (IRHV; 20 mm). The graft RHV and IRHV were formed into a single orifice using the donor's opened round ligament (60 mm × 20 mm) as a patch graft during bench surgery; it was then anastomosed end-to-side with the recipient inferior vena cava. The recipient had no post-transplant complications involving the HVs, but she died of septic shock with persistent cholangitis and jaundice 86 d after LDLT. The HV anastomotic site had no stenosis or thrombus on autopsy. On pathology, there was adequate patency and continuity between the recipient's HV and the donor's opened round ligament. In addition, the stains for CD31 and CD34 on the inner membrane of the opened round ligament were positive. Hepatic venous reconstruction using the opened round ligament as a venous patch graft is effective in LDLT, as observed on pathology.
  • Blumgart-dumpling法による膵空腸吻合の工夫
    小泉 大, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, Lefor Alan, 佐田 尚宏  日本消化器外科学会総会  71回-  P1  -69  2016/07  [Not refereed][Not invited]
  • 小泉大, 田口昌延, 三木厚, 遠藤和洋, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 北山丈二, LEFOR Alan Kawarai, 佐田尚宏  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  28th-  ROMBUNNO.RS14‐3  -479  2016/06  [Not refereed][Not invited]
  • 湯澤真梨子, 三木厚, 利府和馬, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  栃木県医学会々誌  46-  49‐51  -51  2016/06  [Not refereed][Not invited]
  • 膵硬化度・主膵管拡張に注目した膵空腸吻合法の選択と術後膵液瘻の検討
    小泉 大, 田口 昌延, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, アラン・レフォー, 佐田 尚宏  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  479  -479  2016/06  [Not refereed][Not invited]
  • 十二指腸乳頭部神経内分泌癌の一例
    加賀谷 丈紘, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  559  -559  2016/06  [Not refereed][Not invited]
  • 胃壁筋層内へ穿破し出血を生じた膵粘液性嚢胞腫瘍の1例
    太田 学, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  656  -656  2016/06  [Not refereed][Not invited]
  • 肝切除術時の術前貯蔵鉄量が術後肝機能へ及ぼす影響
    山田 直也, 眞田 幸弘, 片野 匠, 岡田 憲樹, 浦橋 泰然, 三木 厚, 笹沼 英紀, 佐久間 康成, 脇屋 太一, 安田 是和, 水田 耕一  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  666  -666  2016/06  [Not refereed][Not invited]
  • 非黄疸胆道閉鎖症における肝移植適応
    眞田 幸弘, 浦橋 泰然, 井原 欣幸, 山田 直也, 岡田 憲樹, 平田 雄大, 片野 匠, 三木 厚, 笹沼 英紀, 佐久間 康成, 佐田 尚宏, 水田 耕一  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  473  -473  2016/06  [Not refereed][Not invited]
  • 十二指腸乳頭部神経内分泌癌の一例
    加賀谷 丈紘, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  559  -559  2016/06  [Not refereed][Not invited]
  • 胃壁筋層内へ穿破し出血を生じた膵粘液性嚢胞腫瘍の1例
    太田 学, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 北山 丈二, 佐田 尚宏  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  656  -656  2016/06  [Not refereed][Not invited]
  • 肝切除術時の術前貯蔵鉄量が術後肝機能へ及ぼす影響
    山田 直也, 眞田 幸弘, 片野 匠, 岡田 憲樹, 浦橋 泰然, 三木 厚, 笹沼 英紀, 佐久間 康成, 脇屋 太一, 安田 是和, 水田 耕一  日本肝胆膵外科学会・学術集会プログラム・抄録集  28回-  666  -666  2016/06  [Not refereed][Not invited]
  • 湯澤真梨子, 三木厚, 利府数馬, 遠藤和洋, 小泉大, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本外科学会定期学術集会(Web)  116th-  RS-1-5 (WEB ONLY)  -1  2016/04  [Not refereed][Not invited]
  • 倉科憲太郎, 三木厚, 松本健司, 村越美穂, 佐藤敏子, 小暮亜弥子, 齋藤心, 鯉沼広治, 笹沼英紀, 小泉大, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本外科学会定期学術集会(Web)  116th-  PS-173-3 (WEB ONLY)  -173  2016/04  [Not refereed][Not invited]
  • 小泉大, 田口昌延, 三木厚, 遠藤和洋, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本外科学会定期学術集会(Web)  116th-  PS-183-4 (WEB ONLY)  -183  2016/04  [Not refereed][Not invited]
  • 腹腔鏡下膵体尾部切除術の完全腹腔鏡下手術例と小切開併用手術例の比較検討
    吉田 淳, 佐久間 康成, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 堀江 久永, 細谷 好則, 佐田 尚宏  日本内視鏡外科学会雑誌  20-  (7)  OS254  -4  2015/12  [Not refereed][Not invited]
  • 腹腔鏡下肝切除にRFAを併用して治療した肝細胞癌2例
    森嶋 計, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏  日本内視鏡外科学会雑誌  20-  (7)  OS277  -5  2015/12  [Not refereed][Not invited]
  • 利府数馬, 三木厚, 遠藤和洋, 小泉大, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本臨床外科学会雑誌  76-  (増刊)  699  -699  2015/10  [Not refereed][Not invited]
  • 倉科憲太郎, 三木厚, 松本健司, 鯉沼広治, 齋藤心, 小泉大, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本臨床外科学会雑誌  76-  (増刊)  702  -702  2015/10  [Not refereed][Not invited]
  • 田口昌延, 笠原尚哉, 森嶋計, 石黒保直, 兼田裕司, 三木厚, 遠藤和洋, 小泉大, 笹沼英紀, 俵藤正信, 佐久間康成, 安田是和, 佐田尚宏  日本臨床外科学会雑誌  76-  (増刊)  983  -983  2015/10  [Not refereed][Not invited]
  • 小泉大, 田口昌延, 三木厚, 遠藤和洋, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本臨床外科学会雑誌  76-  (増刊)  579  -579  2015/10  [Not refereed][Not invited]
  • 遠藤和洋, 太白健一, 三木厚, 小泉大, 笹沼英紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏  日本臨床外科学会雑誌  76-  (増刊)  512  -512  2015/10  [Not refereed][Not invited]
  • 腹部大動脈瘤術後に十二指腸と孔形成した1例
    熊谷 祐子, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 佐田 尚宏  Japanese Journal of Acute Care Surgery  5-  (2)  198  -198  2015/10  [Not refereed][Not invited]
  • 3Dプリンターの医学への応用 消化器外科における3D printerの活用 現況と問題点、その解決案まで
    遠藤 和洋, 三木 厚, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏  日本臨床外科学会雑誌  76-  (増刊)  436  -436  2015/10  [Not refereed][Not invited]
  • 後腹膜気管支原性嚢胞の1例
    坂田 知久, 三木 厚, 利府 数馬, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 佐田 尚宏  日本臨床外科学会雑誌  76-  (増刊)  1146  -1146  2015/10  [Not refereed][Not invited]
  • 胆管内に肝吸虫を認めた肝門部胆管癌の一症例
    菅谷 涼, 佐久間 康成, 三木 厚, 笹沼 英紀, 堀江 久永, 細谷 好則, 佐田 尚宏  日本臨床外科学会雑誌  76-  (増刊)  1168  -1168  2015/10  [Not refereed][Not invited]
  • 山田 直也, 眞田 幸弘, 浦橋 泰然, 井原 欣幸, 岡田 憲樹, 平田 雄大, 田代 昌久, 三木 厚, 笹沼 英紀, 佐久間 康成, 水田 耕一  移植  50-  (総会臨時)  250  -250  2015/09  [Not refereed][Not invited]
  • 「複雑な合流」を呈する膵・胆管合流異常症例から推測される膵・胆管合流異常の発生機序
    眞田 幸弘, 森嶋 計, 笠原 尚哉, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 佐田 尚宏, 安田 是和  日本膵・胆管合流異常研究会プロシーディングス  38-  54  -54  2015/09  [Not refereed][Not invited]
  • 胃癌・大腸癌手術症例の術前に指摘された膵嚢胞性病変の検討
    小泉 大, 佐田 尚宏, 田口 昌延, 森嶋 計, 三木 厚, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 堀江 久永, 細谷 好則, 安田 是和  日本消化器病学会雑誌  112-  (臨増大会)  A881  -A881  2015/09  [Not refereed][Not invited]
  • MORISHIMA KAZUE, KASAHARA NAOYA, ISHIGURO YASUNAO, MIKI ATSUSHI, ENDO KAZUHIRO, KOIZUMI MASARU, SASANUMA HIDEKI, SAKUMA YASUNARI, YASUDA YOSHIKAZU, SATA NAOHIRO  胆道  29-  (3)  566  -566  2015/08  [Not refereed][Not invited]
  • 膵管内乳頭粘液性腫瘍のテロメア長測定による悪性度診断法の新規開発
    三木 厚, 佐久間 康成, 笹沼 英紀, 森嶋 計, 笠原 尚哉, 大澤 英之, 眞田 幸広, 田久保 海誉, 佐田 尚宏, 安田 是和  日本消化器外科学会総会  70回-  O  -11  2015/07  [Not refereed][Not invited]
  • 早期十二指腸乳頭部癌治療戦略における縮小手術の位置づけ
    小泉 大, 佐田 尚宏, 田口 昌延, 笠原 尚哉, 森嶋 計, 三木 厚, 笹沼 英紀, 佐久間 康成, 安田 是和  日本消化器外科学会総会  70回-  P  -196  2015/07  [Not refereed][Not invited]
  • AOKI YUICHI, MIKI ATSUSHI, MORISHIMA KAZUE, SASANUMA HIDEKI, SAKUMA YASUNARU, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  27th-  ROMBUNNO.P67-3  -604  2015/06  [Not refereed][Not invited]
  • SANADA YUKIHIRO, SANADA YUKIHIRO, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  27th-  ROMBUNNO.P98-2  -658  2015/06  [Not refereed][Not invited]
  • YOSHIDA ATSUSHI, MIKI ATSUSHI, MORISHIMA KAZUE, KASAHARA NAOYA, SASANUMA HIDEKI, SAKUMA YASUNARU, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  27th-  ROMBUNNO.P135-3  -722  2015/06  [Not refereed][Not invited]
  • 幽門輪温存膵頭十二指腸切除術術後のドレーンアミラーゼと膵液瘻の検討
    小泉 大, 佐田 尚宏, 田口 昌延, 笠原 尚哉, 森嶋 計, 三木 厚, 笹沼 英紀, 佐久間 康成, 安田 是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  27回-  470  -470  2015/06  [Not refereed][Not invited]
  • 胆管内乳頭状腫瘍(IPNB)切除例についての検討
    森嶋 計, 笠原 尚哉, 石黒 保直, 兼田 裕司, 三木 厚, 黒河内 顕, 笹沼 英紀, 佐久間 康成, 佐田 尚宏, 安田 是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  27回-  716  -716  2015/06  [Not refereed][Not invited]
  • SAITO AKIRA, MIKI ATSUSHI, KASAHARA NAOYA, MORISHIMA KAZUE, TAGUCHI MASANOBU, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  すい臓  30-  (3)  456  -456  2015/05  [Not refereed][Not invited]
  • Noriki Okada, Yukihiro Sanada, Yuta Hirata, Naoya Yamada, Taiichi Wakiya, Yoshiyuki Ihara, Taizen Urahashi, Atsushi Miki, Yuji Kaneda, Hideki Sasanuma, Takehito Fujiwara, Yasunaru Sakuma, Atsushi Shimizu, Masanobu Hyodo, Yoshikazu Yasuda, Koichi Mizuta  Pediatric transplantation  19-  (3)  279  -86  2015/05  [Not refereed][Not invited]
     
    Previous studies have demonstrated the safety of ABO-incompatible pediatric LDLT using preoperative plasmapheresis and rituximab; however, no reports have described the timing and dosage of rituximab administration for pediatric LDLT. This study aimed to describe a safe and effective dosage and timing of rituximab for patients undergoing pediatric ABO-incompatible LDLT based on the experience of our single center. A total of 192 LDLTs in 187 patients were examined. These cases included 29 ABO-incompatible LDLTs in 28 patients. Rituximab was used beginning in January 2004 in recipients older than two yr of age (first period: 375 mg/m(2) in two cases; second period: 50 mg/m(2) in two cases; and 200 mg/m(2) in eight cases). Two patients who received 375 mg/m(2) rituximab died of Pneumocystis carinii pneumonia and hemophagocytic syndrome. One patient who received 50 mg/m(2) rituximab required retransplantation as a consequence of antibody-mediated complications. All eight patients administered 200 mg/m(2) survived, and the mean CD20(+) lymphocyte count was 0.1% at the time of LDLT. In the preoperative management of patients undergoing pediatric ABO-incompatible LDLT, the administration of 200 mg/m(2) rituximab three wk prior to LDLT was safe and effective.
  • 三木 厚, 佐久間 康成, 眞田 幸広, 森嶋 計, 笹沼 秀紀, 福嶋 敬宜, 田久保 海誉, 佐田 尚宏, 安田 是和  膵臓  30-  (3)  269  -269  2015/05  [Not refereed][Not invited]
  • SAITO AKIRA, MIKI ATSUSHI, MORISHIMA KAZUE, KASAHARA NAOYA, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  肝臓  56-  (Supplement 1)  A416  -A416  2015/04  [Not refereed][Not invited]
  • 肝胆膵 門脈塞栓術後の99mTc-GSA-SPECTを用いた残肝機能予測の有用性
    三木 厚, 佐久間 康成, 森嶋 計, 笠原 尚哉, 笹沼 英紀, 佐田 尚宏, 安田 是和  日本外科学会定期学術集会抄録集  115回-  OP  -166  2015/04  [Not refereed][Not invited]
  • Naoya Yamada, Yukihiro Sanada, Yuta Hirata, Noriki Okada, Taiichi Wakiya, Yoshiyuki Ihara, Atsushi Miki, Yuji Kaneda, Hideki Sasanuma, Taizen Urahashi, Yasunaru Sakuma, Yoshikazu Yasuda, Koichi Mizuta  Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society  21-  (2)  233  -8  2015/02  [Not refereed][Not invited]
     
    In the field of pediatric living donor liver transplantation (LDLT), physicians sometimes must reduce the volume of left lateral segment (LLS) grafts to prevent large-for-size syndrome. There are 2 established methods for decreasing the size of an LLS graft: the use of a segment 2 (S2) monosegment graft and the use of a reduced LLS graft. However, no procedure for selecting the proper graft type has been established. In this study, we conducted a retrospective investigation of LDLT and examined the strategy of graft selection for patients weighing ≤6 kg. LDLT was conducted 225 times between May 2001 and December 2012, and 15 of the procedures were performed in patients weighing ≤6 kg. We selected S2 monosegment grafts and reduced LLS grafts if the preoperative computed tomography (CT)-volumetry value of the LLS graft was >5% and 4% to 5% of the graft/recipient weight ratio, respectively. We used LLS grafts in 7 recipients, S2 monosegment grafts in 4 recipients, reduced S2 monosegment grafts in 3 recipients, and a reduced LLS graft in 1 recipient. The reduction rate of S2 monosegment grafts for use as LLS grafts was 48.3%. The overall recipient and graft survival rates were both 93.3%, and 1 patient died of a brain hemorrhage. Major surgical complications included hepatic artery thrombosis in 2 recipients, bilioenteric anastomotic strictures in 2 recipients, and portal vein thrombosis in 1 recipient. In conclusion, our graft selection strategy based on preoperative CT-volumetry is highly useful in patients weighing ≤6 kg. S2 monosegment grafts are effective and safe in very small infants particularly neonates.
  • KOIZUMI DAI, SATA NAOHIRO, TAGUCHI MASANOBU, KASAHARA NAOYA, MORISHIMA KAZUE, MIKI ATSUSHI, SASANUMA HIDEKI, SAKUMA YASUNARI, YASUDA YOSHIKAZU  日本ヘルニア学会学術集会プログラム・抄録集  13th-  151  2015  [Not refereed][Not invited]
  • Yukihiro Sanada, Hideki Sasanuma, Yasunaru Sakuma, Kazue Morishima, Naoya Kasahara, Yuji Kaneda, Atsushi Miki, Takehito Fujiwara, Atsushi Shimizu, Masanobu Hyodo, Yuta Hirata, Naoya Yamada, Noriki Okada, Yoshiyuki Ihara, Taizen Urahashi, Seiji Madoiwa, Jun Mimuro, Koichi Mizuta, Yoshikazu Yasuda  Pediatric transplantation  18-  (8)  E270-3  2014/12  [Not refereed][Not invited]
     
    The use of donors with coagulation FIX deficiency is controversial, and there are no current protocols for peri-transplant management. We herein describe the first reported case of a pediatric LDLT from an asymptomatic donor with mild coagulation FIX deficiency. A 32-yr-old female was evaluated as a donor for her 12-month-old daughter with biliary atresia. The donor's pretransplant coagulation tests revealed asymptomatic mild coagulation FIX deficiency (FIX activity 60.8%). Freeze-dried human blood coagulation FIX concentrate was administered before the dissection of the liver and 12 h afterwards by bolus infusion (40 U/kg) and was continued on POD 1. The bleeding volume at LDLT was 590 mL. On POD 1, 3, 5, and 13, the coagulation FIX activity of the donor was 121.3%, 130.6%, 114.6%, and 50.2%, respectively. The donor's post-transplant course was uneventful, and the recipient is currently doing well at 18 months after LDLT. The FIX activity of the donor and recipient at nine months after LDLT was 39.2% and 58.0%, respectively. LDLT from donors with mild coagulation FIX deficiency could be performed effectively and safely using peri-transplant short-term coagulation FIX replacement and long-term monitoring of the plasma FIX level in the donor.
  • KUROSAWA AKIRA, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本臨床外科学会雑誌  75-  (増刊)  553  -553  2014/10  [Not refereed][Not invited]
  • TOJO MINEYUKI, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本臨床外科学会雑誌  75-  (増刊)  705  -705  2014/10  [Not refereed][Not invited]
  • AOKI YUICHI, MIKI ATSUSHI, KASAHARA NAOYA, MORISHIMA KAZUE, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本臨床外科学会雑誌  75-  (増刊)  675  -675  2014/10  [Not refereed][Not invited]
  • MORISHIMA KAZUE, KUROSAWA AKIRA, KASAHARA NAOYA, MIKI ATSUSHI, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本臨床外科学会雑誌  75-  (増刊)  548  -548  2014/10  [Not refereed][Not invited]
  • SASANUMA HIDEKI, SATA NAOHIRO, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SAKUMA YASUNARI, YASUDA YOSHIKAZU  日本内視鏡外科学会雑誌  19-  (7)  676  -676  2014/10  [Not refereed][Not invited]
  • SAITO AKIRA, MIKI ATSUSHI, MORISHIMA KAZUE, KASAHARA NAOYA, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本膵・胆管合流異常研究会プロシーディングス  37th-  66  -66  2014/09  [Not refereed][Not invited]
  • AOKI YUICHI, SASANUMA HIDEKI, KASAHARA NAOYA, MORISHIMA KAZUE, MIKI ATSUSHI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  胆道  28-  (3)  597  -597  2014/08  [Not refereed][Not invited]
  • YOSHIDA JUN, SAKUMA YASUNARI, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SASANUMA HIDEKI, SATA NAOHIRO, YASUDA YOSHIKAZU  小切開・鏡視外科学会雑誌  5-  (1)  45  -45  2014/08  [Not refereed][Not invited]
  • 肝細胞癌肝切除症例に対する残肝機能測定とリスク評価
    三木 厚, 佐久間 康成, 森島 計, 石黒 保直, 兼田 裕司, 黒河内 顕, 笹沼 英紀, 佐田 尚宏, 安田 是和  日本消化器外科学会総会  69回-  O  -4  2014/07  [Not refereed][Not invited]
  • 肝門部胆管癌術後の放射線治療の検討
    森嶋 計, 佐久間 康成, 三木 厚, 兼田 裕司, 石黒 保直, 黒河内 顕, 笹沼 英紀, 佐田 尚宏, 安田 是和  日本消化器外科学会総会  69回-  P  -18  2014/07  [Not refereed][Not invited]
  • 中部胆管癌に対する肝外胆管切除術の適応
    松本 志郎, 佐田 尚宏, 兼田 裕司, 三木 厚, 石黒 保直, 黒河内 顕, 小泉 大, 笹沼 英紀, 佐久間 康成, 安田 是和  日本消化器外科学会総会  69回-  RS  -16  2014/07  [Not refereed][Not invited]
  • 幽門輪温存膵頭十二指腸切除術後のドレーン排液アミラーゼによるドレーン管理
    小泉 大, 佐田 尚宏, 森 和亮, 森嶋 計, 石黒 保直, 兼田 裕司, 三木 厚, 笹沼 英紀, 佐久間 康成, 安田 是和  日本消化器外科学会総会  69回-  RS  -43  2014/07  [Not refereed][Not invited]
  • KUROKOUCHI KEN, SATA NAOHIRO, MORISHIMA KAZUE, ISHIGURO YASUNAO, MIKI ATSUSHI, KANEDA YUJI, ENDO KAZUHIRO, KOIZUMI DAI, SASANUMA HIDEKI, SAKUMA YASUNARI, YASUDA YOSHIKAZU  すい臓  29-  (3)  660  -660  2014/06  [Not refereed][Not invited]
  • KOIZUMI MASARU, SATA NAOHIRO, TAGUCHI MASANOBU, MORISHIMA KAZUE, ISHIGURO YASUNAO, KANEDA YUJI, MIKI ATSUSHI, KUROGOUCHI AKIRA, SASANUMA HIDEKI, SAKUMA YASUNARI, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  26th-  ROMBUNNO.PRS10-1  -423  2014/06  [Not refereed][Not invited]
  • KOBAYASHI HISAYA, MIKI ATSUSHI, MORISHIMA KAZUE, KANEDA YUJI, ISHIGURO YASUNAO, KUROGOUCHI AKIRA, SASANUMA HIDEKI, SAKUMA YASUNARI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  26th-  ROMBUNNO.P83-5  -599  2014/06  [Not refereed][Not invited]
  • TAGUCHI MASANOBU, SANADA YUKIHIRO, KASAHARA NAOYA, MORISHIMA KAZUE, ISHIGURO YASUNAO, KANEDA YUJI, MIKI ATSUSHI, KUROGOUCHI AKIRA, ENDO KAZUHIRO, KOIZUMI MASARU, SASANUMA HIDEKI, FUJIWARA TAKETO, SAKUMA YASUNARI, SHIMIZU ATSUSHI, HYODO MASANOBU, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  26th-  ROMBUNNO.P116-2  -639  2014/06  [Not refereed][Not invited]
  • KANEDA YUJI, SATA NAOHIRO, TAGUCHI MASANOBU, MORISHIMA KAZUE, ISHIGURO YASUNAO, MIKI ATSUSHI, KOIZUMI MASARU, SASANUMA HIDEKI, SAKUMA YASUNARU, YASUDA YOSHIKAZU  日本肝胆膵外科学会学術集会プログラム・抄録集(CD-ROM)  26th-  ROMBUNNO.P124-2  -649  2014/06  [Not refereed][Not invited]
  • 当施設における生体肝移植後胆管空腸吻合部閉塞に対するRendezvous penetration methodの治療成績
    眞田 幸弘, 川野 陽一, 矢野 智則, 畑中 恒, 浦橋 泰然, 井原 欣幸, 岡田 憲樹, 山田 直也, 平田 雄大, 森嶋 計, 三木 厚, 兼田 裕司, 笹沼 英紀, 佐久間 康成, 安田 是和, 山本 博徳, 水田 耕一  日本肝胆膵外科学会・学術集会プログラム・抄録集  26回-  425  -425  2014/06  [Not refereed][Not invited]
  • 原発性十二指腸癌のムチンタンパク発現による臨床病理的解析
    三木 厚, 佐久間 康成, 笹沼 英紀, 黒河内 顕, 兼田 祐司, 石黒 保直, 森島 計, 佐田 尚宏, 安田 是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  26回-  464  -464  2014/06  [Not refereed][Not invited]
  • 胆管内乳頭状腫瘍(IPNB)切除例の検討
    森嶋 計, 佐久間 康成, 石黒 保直, 兼田 裕司, 三木 厚, 黒河内 顕, 笹沼 英紀, 佐田 尚宏, 安田 是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  26回-  719  -719  2014/06  [Not refereed][Not invited]
  • OKADA NORIKI, SANADA YUKIHIRO, URAHASHI TAIZEN, IHARA YOSHIYUKI, YAMADA NAOYA, HIRATA TAKEHIRO, MIKI ATSUSHI, KANEDA YUJI, SASANUMA HIDEKI, SAKUMA YASUNARI, YASUDA YOSHIKAZU, MIZUTA KOICHI  日本外科学会雑誌  115-  (臨増2)  320  -320  2014/03  [Not refereed][Not invited]
  • 岡田 憲樹, 眞田 幸弘, 浦橋 泰然, 井原 欣幸, 山田 直也, 平田 雄大, 三木 厚, 兼田 裕司, 笹沼 英紀, 佐久間 康成, 安田 是和, 水田 耕一  日本外科学会雑誌  115-  (2)  320  -320  2014/03  [Not refereed][Not invited]
  • 小泉 大, 佐田 尚宏, 眞田 幸弘, 田口 昌延, 石黒 保直, 兼田 裕司, 三木 厚, 黒河内 顕, 遠藤 和洋, 笹沼 英紀, 佐久間 康成, 太田 真, Lefor Alan, 安田 是和  日本外科学会雑誌  115-  (2)  918  -918  2014/03  [Not refereed][Not invited]
  • KOIZUMI DAI, SATA NAOHIRO, SANADA YUKIHIRO, TAGUCHI MASANOBU, ISHIGURO YASUNAO, KANEDA YUJI, MIKI ATSUSHI, KUROKOUCHI KEN, ENDO KAZUHIRO, SASANUMA HIDEKI, SAKUMA YASUNARI, OTA MAKOTO, LEFOR ALAN, YASUDA YOSHIKAZU  日本外科学会雑誌  115-  (臨増2)  918  -918  2014/03  [Not refereed][Not invited]
  • Bevacizumab併用化学療法中に二度の消化管穿孔を発症した直腸癌の1例
    佐田友 藍, 鯉沼 広治, 三木 厚, 堀江 久永, 安田 是和  癌と化学療法  40-  (7)  943  -945  2013/07  [Not refereed][Not invited]
     
    症例は64歳の男性。直腸癌、腹膜播種、肺転移に対して低位前方切除術を施行後mFOLFOX6+bevacizumab(BV)療法を導入した。28コース目のBV投与26日目に腹痛、発熱が出現、CT検査で骨盤内膿瘍とfree air、虫垂内の石灰化を認め緊急手術を施行した。虫垂穿孔による膿瘍形成を認め虫垂切除・膿瘍ドレナージ術を施行した。術後2日目に骨盤内留置ドレーンより腸液の流出を認め、再手術を行ったところ回腸穿孔を認めた。穿孔部は境界明瞭な円形であり、播種など腫瘍性変化は認めず、単純閉鎖術を行った。再手術後は経過良好で、初回術後19日で退院した。虫垂の病理学的所見は直腸癌播種の腫瘍崩壊による穿孔を示唆していた。消化管穿孔はBVの重篤な副作用の一つである。本症例では短期間に二度、異なる部位に消化管穿孔を認め貴重な症例と考え報告する。(著者抄録)
  • 膵頭十二指腸切除後胆管炎・胆管狭窄に対する治療戦略
    三木 厚, 佐久間 康成, 笹沼 英紀, 遠藤 和洋, 兼田 裕司, 佐田 尚宏, 安田 是和  日本消化器外科学会総会  68回-  O  -51  2013/07  [Not refereed][Not invited]
  • 当院にて手術施行した十二指腸水平部癌の4例
    青木 裕一, 佐久間 康成, 三木 厚, 石黒 保直, 遠藤 和洋, 笹沼 英紀, 佐田 尚宏, 安田 是和  日本消化器外科学会総会  68回-  P  -90  2013/07  [Not refereed][Not invited]
  • MIKI ATSUSHI, SAKUMA YASUNARI, ENDO KAZUHIRO, SASANUMA HIDEKI, SATA NAOHIRO, YASUDA YOSHIKAZU  すい臓  28-  (3)  472  2013/06  [Not refereed][Not invited]
  • SHIMIZU ATSUSHI, HYODO MASANOBU, SAKUMA YASUNARI, FUJIWARA TAKEHITO, SASANUMA HIDEKI, ENDO KAZUHIRO, ISHIGURO YASUNAO, SANADA YUKIHIRO, KANEDA YUJI, MORISHIMA KAZUE, MIKI ATSUSHI, KASAHARA NAOYA, TAGUCHI MASANOBU, YAMASHITA KEISUKE, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会・学術集会プログラム・抄録集  25th-  452  -452  2013/06  [Not refereed][Not invited]
  • 清水 敦, 佐久間 康成, 笹沼 英紀, 遠藤 和洋, 小泉 大, 俵藤 正信, 藤原 岳人, 眞田 幸弘, 兼田 裕司, 三木 厚, 森嶋 計, 笠原 尚哉, 田口 昌延, 佐田 尚宏, 安田 是和  日本外科学会雑誌  114-  (2)  653  -653  2013/03  [Not refereed][Not invited]
  • 清水 敦, 佐久間 康成, 笹沼 英紀, 遠藤 和洋, 小泉 大, 俵藤 正信, 藤原 岳人, 眞田 幸弘, 兼田 裕司, 三木 厚, 森嶋 計, 笠原 尚哉, 田口 昌延, 佐田 尚宏, 安田 是和  日本外科学会雑誌  114-  (2)  2013/03  [Not refereed][Not invited]
  • 三木 厚, 佐久間 康成, 佐田 尚宏, 安田 是和  日本外科学会雑誌  114-  (2)  2013/03  [Not refereed][Not invited]
  • SASANUMA HIDEKI, SATA NAOHIRO, KOIZUMI DAI, TAGUCHI MASANOBU, MORISHIMA KAZUE, KASAHARA NAOYA, KANEDA YUJI, MIKI ATSUSHI, SAKUMA YASUNARI, SHIMIZU ATSUSHI, FUKUSHIMA NORIYOSHI, YASUDA YOSHIKAZU  日本臨床外科学会雑誌  73-  (増刊)  459  -459  2012/10  [Not refereed][Not invited]
  • 大腸癌手術時に診断した総胆管結石を伴った先天性胆嚢欠損症の1例
    太白 健一, 栗原 克己, 芝 順太郎, 黒河内 顕, 三木 厚, 斉藤 心, 平嶋 勇希, 笹沼 英紀, 安田 是和  日本消化器外科学会総会  67回-  1  -1  2012/07  [Not refereed][Not invited]
  • SASANUMA HIDEKI, SATA NAOHIRO, KOIZUMI DAI, TAGUCHI MASANOBU, MORISHIMA KAZUE, KASAHARA NAOYA, KANEDA YUJI, MIKI ATSUSHI, ENDO KAZUHIRO, SAKUMA YASUNARI, SHIMIZU ATSUSHI, FUKUSHIMA NORIYOSHI, YASUDA YOSHIKAZU  すい臓  27-  (3)  307  2012/05  [Not refereed][Not invited]
  • SHIMIZU ATSUSHI, HYODO MASANOBU, SAKUMA YASUNARI, SASANUMA HIDEKI, FUJIWARA TAKEHITO, ENDO KAZUHIRO, TAGUCHI MASANOBU, SANADA YUKIHIRO, MORISHIMA KAZUE, KASAHARA NAOYA, MIKI ATSUSHI, SHIMURA KUNIHIKO, KANEDA YUJI, SATA NAOHIRO, YASUDA YOSHIKAZU  日本肝胆膵外科学会・学術集会プログラム・抄録集  24th-  500  -500  2012/05  [Not refereed][Not invited]
  • 慢性膵炎は治療介入により改善するか? アルコール性慢性膵炎における飲酒基準 断酒・禁煙による治療介入
    佐田 尚宏, 田口 昌延, 森嶋 計, 眞田 幸弘, 兼田 裕司, 三木 厚, 遠藤 和洋, 小泉 大, 笹沼 英紀, 佐久間 康成, 清水 敦, 安田 是和  膵臓  27-  (3)  356  -356  2012/05  [Not refereed][Not invited]
  • 遠藤 和洋, 佐田 尚宏, 田口 昌延, 兼田 裕司, 三木 厚, 小泉 大, 笹沼 英紀, 藤原 岳人, 佐久間 康成, 清水 敦, 佐藤 正信, 安田 是和  日本外科学会雑誌  113-  (2)  350  -350  2012/03  [Not refereed][Not invited]
  • 佐田 尚宏, 小泉 大, 志村 国彦, 兼田 裕司, 笠原 尚哉, 森嶋 計, 田口 昌延, 三木 厚, 遠藤 和洋, 藤原 岳人, 清水 敦, 俵藤 正信, 安田 是和  日本外科学会雑誌  113-  (2)  2012/03  [Not refereed][Not invited]
  • 清水 敦, 俵藤 正信, 佐久間 康成, 藤原 岳人, 遠藤 和洋, 三木 厚, 笹沼 英紀, 森嶋 計, 田口 昌延, 笠原 尚哉, 小泉 大, 兼田 裕司, 山下 圭輔, 佐田 尚宏, 安田 是和  日本外科学会雑誌  113-  (2)  547  -547  2012/03  [Not refereed][Not invited]
  • 田口昌延, 俵藤正信, 森嶋計, 三木厚, 遠藤和洋, 兼田裕司, 小泉大, 藤原岳人, 清水敦, 佐田尚宏, 安田是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  24th-  412  -412  2012  [Not refereed][Not invited]
  • 三木厚, 伊藤誉, 清水敦, 俵藤正信, 佐田尚宏, 安田是和  日本臨床外科学会雑誌  72-  (増刊)  818  -818  2011/10  [Not refereed][Not invited]
  • 伊藤誉, 三木厚, 小泉大, 佐田尚宏, 安田是和, 山口岳彦, 坂谷貴司, 福島敬宜  日本臨床外科学会雑誌  72-  (増刊)  869  -869  2011/10  [Not refereed][Not invited]
  • 遠藤和洋, 佐田尚宏, 田口昌延, 兼田裕司, 三木厚, 小泉大, 藤原岳人, 清水敦, 俵藤正信, 安田是和  日本臨床外科学会雑誌  72-  (増刊)  327  -327  2011/10  [Not refereed][Not invited]
  • 三木厚, 田口昌延, 笠原尚哉, 森嶋計, 兼田裕司, 小泉大, 藤原岳人, 佐久間康成, 太田真, 清水敦, 俵藤正信, 佐田尚宏, 安田是和  日本臨床外科学会雑誌  72-  (増刊)  621  -621  2011/10  [Not refereed][Not invited]
  • 三木 厚, 小泉 大, 笠原 尚哉, 兼田 祐司, 藤原 岳人, 佐久間 康成, 太田 真, 俵藤 正信, 佐田 尚宏, 安田 是和  日本外科学会雑誌  112-  (1)  2011/05  [Not refereed][Not invited]
  • 帖地健, 三木厚, 佐田友藍, 宮倉安幸, 佐田尚宏, 安田是和  日本腹部救急医学会雑誌  31-  (2)  396  2011/02  [Not refereed][Not invited]
  • 三木厚, 佐久間康成, 兼田祐司, 小泉大, 藤原岳人, 俵藤正信, 佐田尚宏, 安田是和  日本肝胆膵外科学会・学術集会プログラム・抄録集  23rd-  370  2011  [Not refereed][Not invited]
  • 三木 厚, Ricordi Camillo, 佐久間 康成, Barker Scott, 三澤 良介, 佐田 尚宏, 安田 是和, 市井 啓仁  日本外科学会雑誌  111-  (2)  2010/03  [Not refereed][Not invited]
  • 三木厚, 佐久間康成, 藤原岳人, 石川暢夫, 八木沢隆, 佐田尚宏, 安田是和  日本臨床腎移植学会プログラム・抄録集  43rd-  125  2010  [Not refereed][Not invited]
  • 佐久間 康成, 山元 俊行, 三木 厚, 安田 是和, Ricordi Camillo, 市井 啓仁  日本外科学会雑誌  109-  (2)  2008/04  [Not refereed][Not invited]

Research Grants & Projects

  • Intrathoracic duct chemotherapy targeted to metastatic abdominal lymph nodes
    Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
    Date (from‐to) : 2018/06 -2021/03 
    Author : 北山 丈二, 山口 博紀, 三木 厚, 寺谷 工, 宮戸 秀世
     
    広汎に多発リンパ節転移をきたし、根治切除不能な消化器癌に対し、頸胸部から胸管内にカテーテルを挿入、そこから逆行性に抗癌剤を持続注入し、広汎な腹部 リンパ節転移巣に高濃度の薬剤を選択的に投与することで、高い抗腫瘍効果と毒性の軽減をはかる「逆行性リンパ管内化学療法」という新規治療戦略の可能性を探ることを目的とした。 (1)麻酔下に大動物のブタの胃壁内および腹部リンパ節にパテントブルーを注入しリンパ管系を容易に視認できるようにした後、開胸し、胸管を同定することを試みた。その結果、腹腔リンパ本管は横隔膜を通過した後、枝分かれして大動脈周囲を上行し、気管分岐部から頭側で再び集合し集合リンパ管を形成することが解った。そこで、頸胸部の気管左方で胸管をテーピングし、カニュレーションをする動物モデルを作成した。 (2)上記モデルにて、脂溶性抗癌剤パクリタキセルを胸管チューブより投与、薬物を投与2時間後の各臓器のパクリタキセル濃度をLC-Massを用いて測定したところ、胃、肝臓、腹部リンパ節、および大網中などの腹部臓器中のパクリタキセル濃度は、末梢静脈から投与した時と比べて有意に高値を示し、有用な投与法となる可能性が示唆された。 (3)開胸後は長時間維持することが困難であることが判明し、より侵襲の少ない頸部で胸管を同定し、カニュレーションするモデルを作成し、投与後24時間までの薬物動態を測定する実験系を作成する事を試みている。
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2017/04 -2020/03 
    Author : KITAYAMA JOJI
     
    Exosomes were isolated from peritoneal fluids of patients of gastric cancer with or without peritoneal metastasis (PM) and comprehensive miRNA expression analysis was performed. In initial screening, we identified 11 dysregulated miRNAs in PM (+) samples. Validation analysis revealed that 4 miRNAs (miR-21-5p, miR-92a-3p, miR-223-3p, and miR-342-3p) were significantly upregulated in 12 PM (+) samples, and their expression levels showed positive correlation with peritoneal cancer index. In contrast, miR-29 family were all downregulated in patients with PM (+) samples. Moreover, miR-29b at gastrectomy was lower in patients with peritoneal recurrence. Ratios of miR-21-5p/miR-29b-3p and miR-223-3p/miR-29b-3p were inversely associated with tumor response and overall survival of the patients who received intraperitoneal chemotherapy. Expression pattern of miRNAs in peritoneal exosome well reflects the tumor burden in peritoneal cavity and could be useful biomarker in the treatment of PM.
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2017/04 -2020/03 
    Author : Sata Naohiro
     
    This study aimed to contribute to a novel therapeutic strategy and stratification of cancer drug therapy by predicting the therapeutic effect and side effects of postoperative adjuvant chemotherapy for pancreatic cancer using microRNA expression. Patients with no recurrence for more than 5 years and patients with recurrence within 2 years were selected among patients who received postoperative adjuvant chemotherapy after curative resection of pancreatic cancer. The relationship between microRNA expression and clinicopathological factors, prognosis, and therapeutic effect were investigated. A comprehensive analysis of microRNA expression revealed that there was no significant difference in expression between the relapsed group and the non-relapsed group and the miRNA expression profiles could not stratify the therapeutic effect of postoperative adjuvant chemotherapy of pancreatic cancer.
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2016/04 -2019/03 
    Author : Ohzawa Hideyuki
     
    We performed comprehensive microRNA analysis using pre-treatment needle biopsy specimens from patients with breast cancer received neoadjuvant chemotherapy, in order to clarify microRNAs associated with drug resistance. In candidate miRNAs, miR-205, which was highly expressed in cases with non-pathologically complete response, altered drug sensitivity and enhanced IL-6 secretion in estrogen receptor positive HER2-positive breast cancer cell lines. A positive feedback was thought to exist in this mechanism. This study has shown that the network of microRNAs and inflammatory cytokines have associated with drug resistance and might be one of novel diagnostic and therapeutic methods.
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2014/04 -2018/03 
    Author : SAKUMA YASUNARU
     
    Brown adipocytes play a central role in regulating metabolism and generating metabolic signals. They are also important to maintain metabolic homeostasis by adjusting energy expenditure. However, these cells are not yet well-understood and the tissue-specific presence of uncoupling protein 1 (UCP-1) is a promising lead to gain further understanding. Pheocromocytomas are known to have a tendency to grow into surrounding adipose tissue and become brown adipose tissue. We have successfully collected and cultured these cells from a patient with a pheocromocytoma. There is very little knowledge about Brown adipocytes except for the presence of UCP-1. We performed fluorescence-activated cell sorting combined with a UCP-1 specific aptamer. Cell surface makers were detected using gene-tip analysis and showed CD9, CD177, CD200:positive、and CD40, CD44, CD109: negative.
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2014/04 -2017/03 
    Author : Kasahara Naoya
     
    We made short bowel syndrome model rat by resecting of 2/3 small intestine to examine the efficacy of polyamine. In this rodent short bowel syndrome model, polyamine intake orally increase spermidine and spermine in intestine and blood. Additionally, in parallel with the concentration of dietary polyamines, progression of remnant jejunal villous height and IgA elevation in whole blood and feces are confirmed. Furthermore, histological findings revealed dietary polyamines may delay hepatic steatosis which develop as a late complication of short bowel syndrome. It is well known that IgA content in blood and feces correlate positively with gut barrier function. Polyamine intake may improve survival by activating the function of remnant intestine resulting in independence of parenteral nutrition.
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2013/04 -2017/03 
    Author : Miki Atsushi
     
    Islet transplantation is a promising therapy for brittle type 1 diabetes. However shortage of donor becomes a problem. Islet cell is vulnerable to stress insulted from islet isolation to transplantation. Therefore the number of islet cell which are alive finally is very few. We hypothesized that islet co-transplantation with pancreas ductal cell that secrete vascular growth factor can protect the islets from oxidative stress induced by ischemia. We also use newly develop technique with PDX1 derived luciferase system (Gluc). We transplanted 300 hundred Gluc islets into subcapsular of kidney or liver. The outcome was not different between the transplantation site. And co-transplantation was not affect the outcome. The co-transplantation is limited to protect islets from the stress. Therefore further innovation is needed for the success of islet transplantation.


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