Researchers Database

ogawa masanori

    Health Center Professor
Last Updated :2021/11/23

Researcher Information

J-Global ID

Research Interests

  • 産業保健   

Research Areas

  • Life sciences / Hygiene and public health (non-laboratory)
  • Life sciences / Hygiene and public health (laboratory)
  • Life sciences / Hygiene and public health (non-laboratory)
  • Life sciences / Hygiene and public health (laboratory)
  • Life sciences / Healthcare management, medical sociology

Academic & Professional Experience

  • 2019/01 - Today  Jichi Medical University保健センター教授
  • 2015/05 - 2018/12  Jichi Medical University保健センター准教授
  • 2009/04 - 2015/04  Jichi Medical University保健センター講師
  • 2006/04 - 2009/03  東京労災病院産業中毒センター
  • 2001/04 - 2003/03  九州厚生年金病院

Education

  • 2003/04 - 2007/03  University of Occupational and Environmental Health, Japan  Graduate School of Medical Science
  • 1995/04 - 2001/03  University of Occupational and Environmental Health, Japan  School of Medicine

Association Memberships

  • 米国公衆衛生学会   THE JAPANESE SOCIETY OF CLINICAL TOXICOLOGY   JAPANESE SOCIETY OF OCCUPATIONAL MEDICINE AND TRAUMATOLOGY   THE JAPANESE SOCIETY FOR HYGIENE   THE JAPANESE SOCIETY OF INTERNAL MEDICINE   JAPAN SOCIETY FOR OCCUPATIONAL HEALTH   

Published Papers

  • Teppei Sasahara, Koki Kosami, Akio Yoshimura, Ryusuke Ae, Dai Akine, Masanori Ogawa, Yuji Morisawa
    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 27 (2) 329 - 335 2021/02 
    INTRODUCTION: Hand hygiene is crucial for infection control in long-term care facilities for elderly (LTCFEs), because it can be easily implemented in the low-resource settings of LTCFEs. This study investigated the actual status of hand hygiene adherence in LTCFEs, identified the factors inhibiting its appropriate implementation, and evaluated the effectiveness of a hand hygiene promotion program. METHODS: In this before-and-after study, participants were staff members (n = 142) at two LTCFEs in Gunma Prefecture, Japan. We modified the World Health Organization's "five moments for hand hygiene" and assessed participants' hand hygiene adherence rates in four situations: (1) Before touching around a resident's mucous membrane area; (2) Before medical practice or clean/aseptic procedures; (3) After body fluid exposure/risk or after touching around a resident's mucous membrane area; and (4) After touching a resident's contaminated environments. The study was divided into four phases. In Phase 1, participants self-assessed their hand hygiene adherence using a questionnaire. In Phase 2, we objectively assessed participants' pre-intervention adherence rates. In Phase 3, an intervention comprising various hand hygiene promotion measures, such as education and hands-on training on hand hygiene practices and timings, was implemented. In Phase 4, participants' post-intervention adherence rates were objectively measured. RESULTS: Although most participants reported high hand hygiene adherence rate in the self-assessment (93.1%), the pre-intervention evaluation revealed otherwise (16.8%). Participants' post-intervention adherence rates increased for all four situations (77.3%). CONCLUSION: The intervention program helped increase participants' hand hygiene adherence rates, indicating its effectiveness. Similar interventions in other LTCFEs may also improve adherence rates.
  • Masanori Ogawa, Ryusuke Ae, Teppei Sasahara, Dai Akine
    Sangyo eiseigaku zasshi = Journal of occupational health 2020/12 
    OBJECTS: The number of workers dispatched to developing countries has increased recently. The sanitary conditions in these countries are different from those in developed countries and from what the workers are used to. Therefore, health control, especially infection control, is an important consideration for working there. In this study, we investigate workers' needs as well as the occupational physicians' skills concerning working in developing countries. We propose a more effective education system for health control. METHODS: Regarding workers who have lived in developing countries, we surveyed the company profiles, duration of stay, vaccination status, infectious education, medical assistance, and satisfaction with infection control strategies of their employer companies. Regarding occupational physicians, we surveyed their profiles, their experiences in consultation, and advice from/to the workers dispatched to developing countries as well as their suggestive advice for staying there. RESULTS: Factors that contributed to workers' satisfaction with their employment companies were prior education and health consulting services in addition to company size. Many occupational physicians believed that this kind of information should be supplied but they did not have the confidence to provide it. CONCLUSIONS: Workers who are dispatched overseas should receive prior education and access to health consulting services. It is necessary for information providers such as occupational physicians to be knowledgeable in travel medicine.
  • Teppei Sasahara, Ryusuke Ae, Akio Yoshimura, Koki Kosami, Kazumasa Sasaki, Yumiko Kimura, Dai Akine, Masanori Ogawa, Kenji Hamabata, Shuji Hatakeyama, Longzhu Cui
    BMC geriatrics 20 (1) 481 - 481 2020/11 
    BACKGROUND: A high prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization has been reported among residents in geriatric long-term care facilities (LTCFs). Some studies indicate that MRSA might be imported from hospitals into LTCFs via resident transfer; however, other studies report that high MRSA prevalence might be caused by cross-transmission inside LTCFs. We aimed to assess which factors have a large impact on the high MRSA prevalence among residents of geriatric LTCFs. METHODS: We conducted a cohort study among 260 residents of four geriatric LTCFs in Japan. Dividing participants into two cohorts, we separately analyzed (1) the association between prevalence of MRSA carriage and length of LTCF residence (Cohort 1: n = 204), and (2) proportion of residents identified as MRSA negative who were initially tested at admission but subsequently identified as positive in secondary testing performed at ≥2 months after their initial test (Cohort 2: n = 79). RESULTS: Among 204 residents in Cohort 1, 20 (9.8%) were identified as positive for MRSA. Compared with residents identified as MRSA negative, a larger proportion of MRSA-positive residents had shorter periods of residence from the initial admission (median length of residence: 5.5 vs. 2.8 months), although this difference was not statistically significant (p = 0.084). Among 79 residents in Cohort 2, 60 (75.9%) were identified as MRSA negative at the initial testing. Of these 60 residents, only one (1.7%) had subsequent positive conversion in secondary MRSA testing. In contrast, among 19 residents identified as MRSA positive in the initial testing, 10 (52.6%) were negative in secondary testing. CONCLUSIONS: The prevalence of MRSA was lower among residents with longer periods of LTCF residence than among those with shorter periods. Furthermore, few residents were found to become MRSA carrier after their initial admission. These findings highlight that MRSA in LTCFs might be associated with resident transfer rather than spread via cross-transmission inside LTCFs.
  • Yumi Sano, Toru Yoshikawa, Yoshifumi Nakashima, Michiko Kido, Masanori Ogawa, Hiroko Makimoto, Kichiro Matsumoto, Yoshiharu Aizawa
    Sangyo eiseigaku zasshi = Journal of occupational health 62 (3) 115 - 126 1341-0725 2020/05 [Refereed][Not invited]
     
    OBJECTIVES: The objectives of this study were to analyze current trends in occupational health activities by classifying reports from medical facilities in Japan. METHODS: Reports of current workplace-level occupational health activities from medical activities that were collected by the Japan Medical Association Occupational Health Committee were used for the study. Of 5,000 questionnaire forms sent to medical facilities, 1,920 responses were returned. The freely described reports on ongoing occupational health activities contained in these responses were classified according to each of the following aspects of reported activities: 1) details of occupational health activities including main actors in workplace-level actions; and 2) approaches taken for occupational safety and health. The classification of the reports was implemented by a working group comprising selected occupational health practitioners and researchers. RESULTS: Among 1,920 survey responses, 581 valid texts were analyzed. Altogether, 1,044 occupational health activities currently undertaken by the facilities were extracted. The reported activities that were classified according to details of occupational health activities mainly comprised "Measures for preventing overwork, labor management, and work-style reform" (35.7%), "Measures for improving mental health" (21.0%), and "Review of occupational safety and health management systems" (19.3%). Medical facilities implementing "Measures for mental health" alongside "Measures for preventing overwork, labor management, and work-style reform" were reported in 13.2% of the responding medical facilities. "Occupational health professionals or safety and health management staff" (71.7%) were the most frequent main actors of these activities, followed by "Members of the workplace" (18.4%) and "Outsourced experts" (2.4%). "Comprehensive safety and health management" (42.0%) was the most common approach taken for occupational safety and health, followed by "Management focusing on topics" (23.8%) and "Case management" (16.5%). Most of these activities focused on primary prevention aimed at labor management including prevention of overwork, work-style reform, and mental health promotion. Another key trend could be "Teamwork among occupational safety and health staff, workers, and employers at respective workplaces as well as outsourced experts." DISCUSSION: Several key trends were extracted from current occupational health activities at medical facilities. In most cases, these measures were implemented simultaneously. This suggests the importance of combining primary prevention measures for mental health with measures for labor management including prevention of overwork. These activities reflect emerging trends that incorporate teamwork between experts, workers, and employers, and provide new perspectives on workplace-level occupational safety and health activities.
  • 看護師のストレスチェック集団分析における総合健康リスクに与える要因
    小川 真規, 秋根 大
    産業衛生学雑誌 (公社)日本産業衛生学会 62 (臨増) 454 - 454 1341-0725 2020/05
  • 佐野 友美, 吉川 徹, 中嶋 義文, 木戸 道子, 小川 真規, 槇本 宏子, 松本 吉郎, 相澤 好治
    産業衛生学雑誌 (公社)日本産業衛生学会 62 (3) 115 - 126 1341-0725 2020/05 [Refereed][Not invited]
     
    目的:医療機関における産業保健活動について、現場での事例をもとに産業保健活動の傾向や実施主体別の分類を試み、現場レベルでの今後の産業保健活動を進めていくための方向性について検討した。対象と方法:日本医師会産業保健委員会が各医療機関を対象に実施した「医療機関における産業保健活動に関するアンケート調査」調査結果を活用した。自由記載欄に記載された現在取り組んでいる産業保健活動の記述内容を対象とし、複数名の専門家により各施設の産業保健活動の分類を試みた。特に、1.個別対策事例(具体的な取り組み事例・産業保健活動の主体)2.産業保健活動の取り組み方を反映した分類の2点に基づき分類を行い、各特徴について検討した。結果:有効回答数1,920件のうち、581件の自由記載があり、1,044件の個別の産業保健活動が整理された。1.個別対策事例のうち、具体的な取り組み事例については、個別対策毎の分類では「B労務管理・過重労働対策・働き方改革(35.7%)」、「Cメンタルヘルス対策関連(21.0%)」、「A労働安全衛生管理体制強化・見直し(19.3%)」等が上位となった。また、施設毎に実施した取り組みに着目した場合、「B労務管理・過重労働対策・働き方改革関連」と「Cメンタルヘルス対策関連等」を併せて実施している施設が施設全体の13.2%に認められた。産業保健活動の主体による分類では、「a:産業保健専門職・安全衛生管理担当者(71.7%)」が最も多く、「b:現場全体(18.4%)」、「c:外部委託(2.4%)の順となった。2.産業保健活動の取り組み方を反映した分類では(1)包括的管理(42.0%)が最も多く、(2)問題別管理(23.8%)、(3)事例管理(16.5%)の順となった。考察と結論:医療機関における産業保健活動として、過重労働対策を含む労務管理・働き方改革、メンタルヘルス対策への取り組みが多く実践されていた。特に、メンタルヘルスにおける一次予防対策と過重労働における一次予防対策を併せて実施している点、外部の産業保健機関、院内の各種委員会、産業保健専門職とが連携し産業保健活動が進められている点が認められた。厳しい労働環境にある医療機関においても、当面の課題に対処しつつ、医療従事者の健康と安全に関する課題を包括的に解決できる具体的な実践が進められつつある。また、各院内委員会や外部専門家との連携によりチームとして行う産業保健活動の進展が、益々期待される。(著者抄録)
  • B型肝炎ワクチンの効果的な接種方法の検討
    鵜飼 瞳, 齋藤 厚子, 鈴木 喜代美, 秋根 大, 小川 真規
    CAMPUS HEALTH (公社)全国大学保健管理協会 57 (1) 223 - 224 1341-4313 2020/03
  • B型肝炎ワクチンの効果的な接種方法の検討
    鵜飼 瞳, 齋藤 厚子, 鈴木 喜代美, 秋根 大, 小川 真規
    CAMPUS HEALTH (公社)全国大学保健管理協会 57 (1) 223 - 224 1341-4313 2020/03
  • Teppei Sasahara, Masanori Ogawa, Itaru Fujimura, Ryusuke Ae, Koki Kosami, Yuji Morisawa
    Biocontrol science 25 (4) 223 - 230 2020 
    Tap water contamination is a growing concern in healthcare facilities, and despite chlorination, tap water in these facilities contains several pathogenic microorganisms causing healthcare-associated waterborne infections or nosocomial outbreaks. Shower units are particularly prone to contamination as they are conducive for bacterial growth and can even produce bioaerosols containing pathogenic bacteria. Shower units coupled with point-of-use (POU) water filters are a simple and safe option; however, their efficacy has been under-reported. Therefore, we determined the efficacy of showerheads attached with a POU filter capsule in preventing infections in our hospital. We investigated the presence of pathogenic bacteria in water sampled from three shower units. After replacing the original shower units with new ones incorporated with a sterile-grade water filter capsule (0.2 µm; QPoint™), the water samples were analyzed for up to 2 months. The POU filters removed several pathogenic bacteria (Mycobacterium, Pseudomonas, Stenotrophomonas, Aeromonas, and Klebsiella spp.). Filter effectiveness depends on regional water quality and we believe that effective tap water treatment combined with the use of POU filters (introduced at a reasonable cost in healthcare facilities) can considerably minimize waterborne diseases in hospitals and improve patient care.
  • Ogawa M, Akine D, Sasahara T
    Environmental health and preventive medicine 24 (1) 80 - 80 1342-078X 2019/12 [Refereed][Not invited]
     
    BACKGROUND: Two types of recombinant hepatitis B virus (HBV) vaccines are available in Japan. One type uses the antigen from genotype A (Heptavax-II®) and the other uses the antigen from genotype C (Bimmugen®). Potential differences in productivity of the hepatitis B virus surface (HBs) antibody between vaccines have not been studied in detail. We investigated the acquired level of immunity against HBV in association with two vaccines, their administration routes, and patient sex. We present the appropriate inoculation method based on the characteristics of each vaccine. METHODS: Data of 1135 medical and nursing students (481 men and 651 women) were used, each of whom was unvaccinated prior to recruitment and subsequently vaccinated three times prior to the study. The vaccine type and administration route differed according to the university department and enrolling year. The students were categorized into the following three groups: Bimmugen®-subcutaneous group, Heptavax-II®-subcutaneous group, and Heptavax-II®-intramuscular group. The total and sex-segregated positive rates of the HBs antibody among the three groups were compared using Pearson's chi-square test. The effect of time between the HBs antibody test and vaccine administration on the HBs antibody level was also analyzed similarly. RESULTS: The Bimmugen®-subcutaneous group showed the highest positive HBs antibody rate (92.0%) among the three groups. In the Heptavax-II® group, the positive rate was 66.3% in the subcutaneous injection group and 89.1% in the intramuscular injection group. There was a significant difference among these three groups. In terms of sex, women showed a significantly higher average positive rate than men in each group. In terms of effect of time between the HBs antibody test and vaccine administration, no significant differences were observed. CONCLUSIONS: Bimmugen® is associated with more effective HBs antibody production than Heptavax-II® in Japanese students. However, the Heptavax-II® vaccine is an appropriate choice for HBV vaccination in areas where HB is caused predominantly by HBV genotype C. With both vaccines, women tended to acquire more immunogenicity than men. Intramuscular injection may be the preferred administration route due to the possibility of local reactions.
  • Ogawa M, Kabe I, Terauchi Y, Tanaka S
    Journal of occupational health 61 (1) 135 - 142 1341-9145 2019/01 [Refereed][Not invited]
  • Koike S, Isse T, Kawaguchi H, Ogawa M
    Occupational medicine (Oxford, England) 0962-7480 2018/09 [Refereed][Not invited]
  • Ae R, Nakamura Y, Tada H, Kono Y, Matsui E, Itabashi K, Ogawa M, Sasahara T, Matsubara Y, Kojo T, Kotani K, Makino N, Aoyama Y, Sano T, Kosami K, Yamashita M, Oka A
    Journal of epidemiology 28 (6) 300 - 306 0917-5040 2018/06 [Refereed][Not invited]
     
    BACKGROUND: Globally, few published studies have tracked the temporal trend of dioxin levels in the human body since 2000. This study describes the annual trend of dioxin levels in human breast milk in Japanese mothers from 1998 through 2015. METHODS: An observational study was conducted from 1998 through 2015. Participants were 1,194 healthy mothers following their first delivery who were recruited annually in Japan. Breast milk samples obtained from participants were analyzed using gas chromatography and mass spectrometry for dioxins, including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and coplanar polychlorinated biphenyls (PCBs). RESULTS: Mean age was 29.5 years, and 53% of participants were 20-25 years old. A declining trend in total dioxin levels was found, from a peak of 20.8 pg toxic equivalence (TEQ)/g fat in 1998 to 7.2 pg TEQ/g fat in 2014. Data from the last 5 years of the study indicated a plateau at minimal levels. In contrast, an increasing trend was found in the mean age of participants during the last 5 years. Although significantly higher dioxin levels were observed in samples from older participants, an upward trend in dioxin levels was not observed, indicating that dietary and environmental exposure to dioxins had greatly diminished in recent years. CONCLUSIONS: Dioxin levels in human breast milk may be approaching a minimum in recent years in Japan. The findings may contribute to global reference levels for environmental pollution of dioxins, which remains a problem for many developing countries.
  • 関東地区の医療機関における産業保健活動に関する研究
    和田 耕治, 小川 真規, 小森 友貴, 日本産業衛生学会医療従事者のための産業保健研究会
    産業衛生学雑誌 (公社)日本産業衛生学会 60 (臨増) 338 - 338 1341-0725 2018/05
  • Masanori Ogawa, Ryusuke Ae, Teppei Sasahara
    Case Reports in Oncology 10 (3) 851 - 856 1662-6575 2017/11 [Refereed][Not invited]
     
    In the current study, we report a case of a 46-year-old man who presented with sudden abdominal pain and was diagnosed with rupture of hepatic angiosarcoma (HAS). He underwent surgery, but died 13 days after the onset of the abdominal pain. Chronic exposure to carcinogens, such as thorium dioxide, arsenic, vinyl chloride, and radium, is associated with HAS. However, our patient had not been exposed to such carcinogens. He had submitted himself for annual medical checkups since he was employed. His liver was cirrhotic, and medical history data showed that he had had fatty liver for at least 10 years before HAS onset. Although liver cirrhosis may play a role in the occurrence of HAS, the connection of chronic fatty liver in the tumorigenesis remains unclear. Case reports regarding HAS with fatty liver are few. To the best of our knowledge, this is the first case of HAS occurring in a cirrhotic liver that advanced from persistent fatty stage. Given that HAS is a rare tumor, data collection is important for investigating its pathophysiology. Case presentations considering health conditions before HAS onset are limited therefore, we present a case of HAS with annual health checkup data before disease onset.
  • Relationship between Self-Reported Vaccination History and Measles and Rubella Antibody Titers in Medical and Nursing Students
    Ogawa M, Ae R, Sasahara T
    Advances in Infectious Diseases 7 (2) 27 - 36 2017/05 [Refereed][Not invited]
  • Xiaoyi Cui, Mayumi Ohtsu, Nathan Mise, Akihiko Ikegami, Atsuko Mizuno, Takako Sakamoto, Masanori Ogawa, Munehito Machida, Fujio Kayama
    SPRINGERPLUS 5 (1) 885  2193-1801 2016/06 [Refereed][Not invited]
     
    This study aimed to evaluate the relationships between oxidative stress and heavy metal exposure (lead [Pb] and cadmium [Cd]), as well as co-factors such as physical activity and age, in Japanese women. This study was conducted with female subjects from a rural agricultural community in Japan. Subjects were asked to complete lifestyle-related questionnaires and undergo a group health examination. Physical activity, alcohol consumption, body mass index, and other demographic information were collected. Blood and urine samples were collected to measure urinary 8-hydroxydeoxyguanosine (8-OHdG) levels and blood and urinary Cd and Pb concentrations. Urine samples were analyzed using high performance liquid chromatography and flameless atomic absorption spectrometry; blood samples were analyzed using inductively coupled plasma-mass spectrometry. Age, physical activity, and blood and urinary Cd and Pb concentrations were included in structural equation modeling analysis. Two latent factors for heavy metal exposure and physical activity were produced to predict the total influence of the variables. The final model was good: CMIN/DF = 0.775, CFI = 1.000, GFI = 0.975, AGFI = 0.954, RMSEA = 0.000. 8-OHdG levels were positively associated with heavy metal exposure, physical activity, and age (standard beta of path analysis: 0.33, 0.38, and 0.20, respectively). Therefore, oxidative stress is associated with both, environmental and lifestyle factors, in combination with aging.
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289 - 289 1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 (一社)日本衛生学会 71 (Suppl.) S215 - S215 0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 (一社)日本衛生学会 71 (Suppl.) S191 - S191 0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    産業衛生学雑誌 (公社)日本産業衛生学会 58 (臨増) 289  1341-0725 2016/05 [Not refereed][Not invited]
  • 小川 真規, 香山 不二雄
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S215  0021-5082 2016/05 [Not refereed][Not invited]
  • 香山 不二雄, 池上 昭彦, 高木 麻衣, 水野 敦子, 三瀬 名丹, 崔 笑怡, 小川 真規, 吉田 貴彦, 小林 弥生, Zafar Fatmi
    日本衛生学雑誌 日本衛生学会 71 (Suppl.) S191  0021-5082 2016/05 [Not refereed][Not invited]
  • Fujio Kayama, Zafar Fatmi, Akihiko Ikegami, Atsuko Mizuno, Mayumi Ohtsu, Nathern Mise, Xiaoyi Cui, Masanori Ogawa, Takako Sakamoto, Yoshiko Nakagi, Takahiko Yoshida, Ambreen Sahito, Shahla Naeem, Kulsoom Ghias, Hina Zuberi, Kanwal Tariq, Yayoi Kobayashi, Keiko Nohara
    Reviews on Environmental Health 31 (1) 33 - 35 0048-7554 2016/03 [Refereed][Not invited]
     
    Aim: Exposure assessment of lead (Pb) and Arsenic (As) from food, water, and house dust intake were assessed among pregnant women, their children and fetuses in Pakistan and Japan, as well as their body burden of the metals in their blood. Method: Fifty families which included a pregnant woman, a fetus and the 1-3-year-old siblings were recruited in Karachi and Khairpur in Pakistan, and Shimotsuke and Asahikawa in Japan, respectively. Their dietary exposure to Pb and As was measured in 3-day food duplicates and drinking water by ICP-MP. Pb in house dust and respirable dust was evaluated with an energy dispersive X-ray fluorescence spectrometry. Nonradioactive isotope Pb profiles of blood specimens will be compared with those of the exposure origins, such as food duplicates, respirable house dust, the soils nearby, and gasoline. Results: Judging from the data collected and analyzed so far, contribution from dietary intake is highly correlated to higher body burden of Pb among Pakistani mothers. Additional data analyses will reveal the status of Pb and As body burden in Pakistani mothers, fetuses and their siblings, and causal sources of high body burden is delineated by Pb isotope profile analysis of different sources of Pb exposure.
  • Masanori Ogawa, Fujio Kayama
    JOURNAL OF OCCUPATIONAL MEDICINE AND TOXICOLOGY 10 13  1745-6673 2015/03 [Refereed][Not invited]
     
    Background: Aluminum is considered to be a relatively safe metal for humans. However, there are some reports that aluminum can be toxic to humans and animals. In order to estimate the toxicity of aluminum with respect to humans, we measured the aluminum concentration in urine of aluminum-handling and non-handling workers and investigated the relationships between their urinary aluminum concentrations and pre-clinical findings. Methods: Twenty-three healthy aluminum-handling workers and 10 healthy non-aluminum-handling workers participated in this study. Their medical examinations, which were otherwise unremarkable, included the collection of urine and blood. Urinary aluminum levels were analyzed using ICP analysis. As pre-clinical tests, we measured KL-6, SP-D, TRCP-5b, IL-6, and IL-8 in blood and delta-ALA and beta 2-microglobulin in urine. These were considered to be lung, bone, kidney and inflammation markers. Moreover, we measured 8-OHdG in urine as an oxidative DNA damage marker. Results: The aluminum concentration in urine ranged from 6.9 to 55.1 mu g/g cre (median: 20.1 mu g/g cre) in the aluminum-handling workers and from 5.6 to 15.6 mu g/g cre (median: 8.8 mu g/g cre) in the non-aluminum-handling workers, with a significant difference between them. In the pre-clinical findings, there were no significant differences between these two groups except in the case of delta-ALA. However, there were no significant relationships between aluminum concentration and the pre-clinical findings, work years, age or 8-OHdG in the aluminum-handling workers. Conclusions: While the excretion of aluminum in urine was elevated in aluminum-handling workers, our findings suggest that low-dose aluminum is not directly harmful to humans, at least when workers' urinary aluminum concentration is below 55 mu g/g cre.
  • Cimi Ilmiawati, Takahiko Yoshida, Toshihiro Itoh, Yoshihiko Nakagi, Yasuaki Saijo, Yoshihiko Sugioka, Mineshi Sakamoto, Akihiko Ikegami, Masanori Ogawa, Fujio Kayama
    ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE 20 (1) 18 - 27 1342-078X 2015/01 [Refereed][Not invited]
     
    To measure current Hg, Cd, and Pb exposure in Japanese children, and to estimate dietary intakes of foods responsible for high body burden. Blood, hair, and urine samples were collected from 9 to 10-year-old 229 children in Asahikawa and measured for Hg, Cd, and Pb in these matrices. Diet history questionnaire was used to estimate intake of marine foods and other food items. Hg level was measured by cold vapor atomic absorption spectrometry. Cd and Pb levels were determined with inductively coupled plasma mass spectrometry. Geometric mean (GM) of blood Hg, Cd, and Pb was 4.55 mu g/L, 0.34 mu g/L, and 0.96 mu g/dL, respectively. Urinary Cd level was 0.34 mu g/g creatinine (GM) and hair Hg was 1.31 mu g/g (GM). Approximately one-third (35 %) of blood samples had Hg level above the U.S. EPA reference dose (RfD; 5.8 mu g/L). Hair Hg level exceeded U.S. EPA RfD (1.2 mu g/g) in 59 % samples. Children in the upper quartile of blood Hg level had significantly higher intake of large predatory fish species compared to those in the lower quartile of blood Hg. Those with high blood Hg level may be explained by more frequent intake of big predatory fish. Cd and Pb exposure is generally low among Japanese children. As no safety margin exists for Pb exposure and high exposure to MeHg is noted in Japanese population; periodic biomonitoring and potential health risk assessment should continue in high-risk populations, notably among children.
  • Dietary factors and urinary arsenic excretion profiles in Japanese women with high seafood intake
    Ilmiawati C, Ogawa M, Sasaki S, Watanabe T, Kayama F
    Jichi Medical University Journal 36 1 - 11 2013 [Refereed][Not invited]
  • Masanori Ogawa, Teppei Sasahara
    JOURNAL OF OCCUPATIONAL MEDICINE AND TOXICOLOGY 7 (1) 24  1745-6673 2012/12 [Refereed][Not invited]
     
    Background: Biological monitoring is used to assess toluene exposure in medical examinations. The American Conference of Industrial Hygienists, Japanese Society for Occupational Health and Deutsche Forschungsgemeinschaft have proposed various biological exposure determinants, such as toluene in blood and urine, and o-cresol in urine. Toluene in blood is a common biomarker among them. Toluene is a volatile organic solvent; therefore, sample preservation under appropriate conditions before measurement is necessary. However, little study has been done on the stability of toluene in workers' blood samples under conditions simulating those of a medical examination. Finding: We carried out a pilot study on the stability of toluene in blood from humans, according to different methods of sample preservation. Toluene in blood was analyzed by head space-gas chromatography/mass spectrometry. The sealing performance of the vial was examined by using toluene-added blood and the stability of toluene in blood according to the preservation period was examined by using blood from toluene-handling workers, which was collected with vacuum blood tubes. The sealing performance of the headspace vial used in this study was good for three days and toluene in blood in tubes from workers was stable at least within 8 hours up to blood packing at 4 degrees C. Conclusion: We could propose that the collected blood need only be transferred into headspace vials on the collection day and analyzed within a few days, if the samples are preserved at 4 degrees C. Our data size is limited; however, it may be considered basic information for biological monitoring in medical examinations.
  • Masanori Ogawa, Yoshihiro Suzuki, Yoko Endo, Toshihiro Kawamoto, Fujio Kayama
    INDUSTRIAL HEALTH 49 (2) 195 - 202 0019-8366 2011/03 [Refereed][Not invited]
     
    Intake of foods and drinks containing benzoic acid influences the urinary hippuric acid (HA) concentration, which is used to monitor toluene exposure in Japan. Therefore, it is necessary to control the intake of benzoic acid before urine collection. Recently, some reports have suggested that components of coffee, such as chlorogenic, caffeic, and quinic acids are metabolized to HA. In this study, we evaluated the influence of coffee intake on the urinary HA concentration in toluene-nonexposed workers who had controlled their benzoic acid intake, and investigated which components of coffee influenced the urinary HA concentration. We collected urine from IS healthy men who did not handle toluene during working hours, after they had consumed coffee, and we measured their urinary HA concentrations; the benzoic acid intake was controlled in these participants during the study period. The levels of chlorogenic, caffeic, and quinic acids in coffee were analyzed by LC-MS/MS. Urinary HA concentration increased significantly with increasing coffee consumption. Spectrophotometric LC-MS/MS analysis of coffee indicated that it contained chlorogenic and quinic acids at relatively high concentrations but did not contain benzoic acid. Our findings suggest that toluene exposure in coffee-consuming workers may be overestimated.
  • Yoshida T, Ogawa M, Goto H, Ohshita A, Kurose N, Yokosawa F, Hirata M, Endo Y
    Sangyo eiseigaku zasshi = Journal of occupational health 2 53 25 - 32 1341-0725 2011 [Refereed][Not invited]
  • Yoshihiro Suzuki, Yoko Endo, Masanori Ogawa, Shinobu Yamamoto, Akito Takeuchi, Tomoo Nakagawa, Nobuhiko Onda
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877 (29) 3743 - 3747 1570-0232 2009/11 [Refereed][Not invited]
     
    N-Methyl-2-pyrrolidone (NMP) has been used in many industries and biological monitoring of NMP exposure is preferred to atmospheric monitoring in Occupational health. We developed an analytical method that did not include solid phase extraction (SPE) but utilized deuterium-labeled compounds as internal standard for high-performance liquid chromatography-electrospray ionization-mass spectrometry using a C30 column. Urinary concentrations of NMP and its known metabolites 5-hydoxy-N-methyl-2-pyrrolidone (5-HNMP), N-methyl-succinimide (MSI), and 2-hydroxy-N-methylsuccinimide (2-HMSI) were determined in a single run. The method provided baseline separation of these compounds. Their limits of detection in 10-fold diluted urine were 0.0001, 0.006, 0.008, and 0.03 mg/L, respectively. Linear calibration covered a biological exposure index (BEI) for urinary concentration. The within-run and total precisions (CV, %) were 5.6% and 9.2% for NMP, 3.4% and 4.2% for 5-HNMP, 3.7% and 6.0% for MSI, and 6.5% and 6.9% for 2-HMSI. The method was evaluated using international external quality assessment samples, and urine samples from workers exposed to NMP in an occupational area. (C) 2009 Elsevier B.V. All rights reserved.
  • Hsu-Sheng Yu, Tsunehiro Oyama, Toyohi Isse, Kyoko Kitakawa, Masanori Ogawa, Thi-Thu-Phuong Pham, Toshihiro Kawamoto
    TOXICOLOGY MECHANISMS AND METHODS 19 (9) 535 - 540 1537-6516 2009 [Refereed][Not invited]
     
    Acetaldehyde is an intermediate of ethanol oxidation. It covalently binds to DNA, and is known as a carcinogen. Aldehyde dehydrogenase 2 (ALDH2) is an important enzyme that oxidizes acetaldehyde. Approximately 45% of Chinese and Japanese individuals have the inactive ALDH2 genotypes (ALDH2* 2/*2 and ALDH2* 1/*2), and Aldh2 knockout mice appear to be a valid animal model for humans with inactive ALDH2. This review gives an over view of published studies on Aldh2 knockout mice, which were treated with ethanol or acetaldehyde. According to these studies, it was found that Aldh2 -/- mice (Aldh2 knockout mice) are more susceptible to ethanol and acetaldehyde-induced toxicity than Aldh2 +/+ mice (wild type mice). When mice were fed with ethanol, the mortality was increased. When they were exposed to atmospheres containing acetaldehyde, the Aldh2 -/- mice showed more severe toxic symptoms, like weight loss and higher blood acetaldehyde levels, as compared with the Aldh2 +/+ mice. Thus, ethanol and acetaldehyde treatment affects Aldh2 knockout mice more than wild type mice. Based on these findings, it is suggested that ethanol consumption and acetaldehyde inhalation are inferred to pose a higher risk to ALDH2-inactive humans. These results also support that ALDH2-deficient humans who habitually consume alcohol have a higher rate of cancer than humans with functional ALDH2.
  • Yoshihiro Suzuki, Yoko Endo, Masanori Ogawa, Michiko Matsuda, Yoshiaki Nakajima, Nobuhiko Onda, Motoki Iwasaki, Shoichiro Tsugane
    ANALYTICAL SCIENCES 24 (8) 1049 - 1052 0910-6340 2008/08 [Refereed][Not invited]
     
    In order to determine low levels of Cd in urine samples, we tried to remove Mo interference using ICP-MS with a dynamic reaction-cell technique, but failed due to the low sensitivity and the variance with the standards. We then performed solid-phase extraction (SPE) before the ICP-MS measurement. A commercially available chelating resin, NOBIAS PA-1, was used for SPE, and could effectively remove Mo from urine samples, permitting the accurate determination of Cd by ICP-MS. This SPE-ICP-MS method gave 0.012 mu g Cd L-1 as the method limit of quantification, and the mean recovery of Cd spiked with 0.0505 and 5.05 mu g L-1 was 93.1 and 97.6%, respectively.
  • Yoshihiro Suzuki, Yoko Endo, Masanori Ogawa, Yangho Kim, Nobuhiko Onda, Kenzo Yamanaka
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 868 (1-2) 116 - 119 1570-0232 2008/06 [Refereed][Not invited]
     
    Dimethyltin dichloride (DMTC) is widely used as a heat stabilizer in manufacturing the polyvinyl chloride. We previously reported a case of acute DMTC poisoning with neurological manifestations very similar to trimethylated tin JMT) encephalopathy, based on results of speciation analysis of methylated tins in the patient's urine with use of a combination of high performance liquid chromatography and inductively coupled plasma-mass spectrometry (HPLC-ICP-MS), which yielded peaks corresponding to DMT and TMT. In this study, we developed an analytical method to confirm TMT in urine using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and found TMT molecular ion in the patient's urine. (c) 2008 Elsevier B.V. All rights reserved.
  • Koichi Furuhashi, Masanori Ogawa, Yoshihiro Suzuki, Yoko Endo, Yangho Kim, Gaku Ichihara
    Chemical research in toxicology 21 (2) 467 - 71 0893-228X 2008/02 [Refereed][Not invited]
     
    Organotins are widely used as stabilizers of polyvinyl chloride and as catalysts or biocides. It is well known that dimethyltin (DMT) is less neurotoxic than trimethyltin (TMT). A Korean worker who was exposed to DMT compounds showed neurological symptoms similar to those of TMT encephalopathy, in association with high levels of both DMT and TMT in the urine and blood. The case suggested the possibility of the methylation of DMT in humans. Here, we investigated whether TMT is detected in the urine of mice and rats exposed only to DMT dichloride (DMTC). Three Slc:ICR mice and three Slc:Wistar rats were placed in individual metabolic cages, and one day later, they were injected intraperitoneally with DMTC (10 mg/kg body weight (wt); 5.4 mgSn/kg body wt; 45.5 micromol/kg body wt) over 4 consecutive days. Twenty-four hour urine samples were collected every evening for 11 consecutive days starting at baseline (before treatment). Speciation analyses of methyltin compounds in urine were performed using a combination of high performance liquid chromatograph-inductively coupled plasma mass spectrometry. High concentrations of DMT and time-dependent increase in TMT concentrations were found in both mice and rats during the 4-day treatment, and their concentrations decreased gradually after the cessation of treatment. The chemical compound of the detected peak was confirmed to be TMT by liquid chromatography-tandem mass spectrometry. Neither DMT nor TMT was detected in the samples collected at baseline. Our results indicate urinary excretion of TMT in mice and rats injected with DMTC, confirming the production of TMT in vivo, probably through methylation of DMT.
  • Naoki Kunugita, Toyohi Isse, Tsunehiro Oyama, Kyoko Kitagawa, Masanori Ogawa, Tetsunosuke Yamaguchi, Tsuyoshi Kinaga, Toshihiro Kawamoto
    JOURNAL OF TOXICOLOGICAL SCIENCES 33 (1) 31 - 36 0388-1350 2008/02 [Refereed][Not invited]
     
    Aldehyde dehydrogenase-2 (ALDH2) metabolizes acetaldehyde produced from ethanol into acetate and plays a major role in the oxidation of acetaldehyde in vivo. About half of all Japanese people have inactive ALDH2. We generated homozygous Aldh2 null (Aldh2-/-) mice by gene targeting knockout as a model of ALDH2-deficient humans. To investigate the mutagenicity of acetaldehyde, a micronucleus assay and a T-cell receptor (TCR) gene mutation assay were performed in Aldh2-/- mice and wild-type (Aldh2+/+) mice exposed to acetaldehyde. The mice were continuously exposed to 125 and 500 ppm of acetaldehyde vapor for 2 weeks. Another group was orally administered 100 mg/k once a day for 2 weeks continuously. The mice were killed after 2 weeks of exposure to acetaldehyde, and the frequency of micronucleated reticulocytes was measured by flow cytometry. We also observed the incidence of TCR gene mutations in T-lymphocytes by measuring the variant CD3(-)CD4(+) expression by flow cytometry. The frequency of micronucleated reticulocytes induced by acetaldehyde was significantly increased in Aldh2-/- mice, but not in Aldh2+/+ mice. TCR mutant frequency was also associated with acetaldehyde exposure in Aldh2-/- mice, especially after oral administration however, it was not associated with acetaldehyde exposure in Aldh2+/+ mice. In conclusion, Aldh2-/- mice showed high sensitivity in the micronuclei and TCR mutation assays compared with Aldh2+/+ mice after exposure to acetaldehyde.
  • Masanori Ogawa, Yoshiaki Nakajima, Ryuichi Kubota, Yoko Endo
    CLINICAL TOXICOLOGY 46 (4) 332 - 335 1556-3650 2008 [Refereed][Not invited]
     
    Case Reports. We experienced two cases of acute lead poisoning due to occupational exposure to lead. The patients were engaged in stripping off antirust compounds including Pb from a bridge and re-painting it at the same work place. Both patients exhibited colic, arthralgia, and anemia. Blood lead levels were 73.1 mu g/dl and 96.3 mu g/dl. Intravenous CaEDTA chelation therapy was therefore performed. After chelation, blood lead levels decreased and symptoms gradually disappeared. Discussion. Although the patients were working with protective equipment, the workplace was in the mountains and there was no water for washing. The patients were thus unable to washing their hands and faces. We assume that they swallowed lead dust left on their hands and faces when they removed their clothing, and believe that this poisoning occurred due to lack of knowledge sufficient for protection.
  • Tsunehiro Oyama, Yong-Dae Kim, Toyohi Isse, Pham Thi Thu Phuong, Masanori Ogawa, Tetsunosuke Yamaguchi, Tsuyoshi Kinaga, Yasunori Yashima, Heon Kim, Toshihiro Kawamoto
    Journal of Toxicological Sciences 32 (4) 421 - 428 0388-1350 2007/10 [Refereed][Not invited]
     
    Aldh (aldehyde dehydrogenase) 2 knockout (KO) mice have been generated in our laboratory. We evaluated the effects of subacute ethanol treatment on the survival rate, expression of Aldh1, Aldh2, Cytochrome P450 (Cyp) 1A1, Cyp2e1 and Cyp4b1 in wild (Aldh2 +/+) mice (C57BL/6) and Aldh2 knock out (Aldh2 ?/?) mice. Physiological saline (0.3 mL/day) was administered to 4 Aldh2 +/+ and 4 Aldh2 ?/? mice for 8 days as a control. Forty percent ethanol (0.3 mL/day ethanol 2 g/kg/day) was then administered to 5 Aldh2 +/+ and 9 Aldh2 ?/? mice for 8 days. Three mice of the ethanol administered Aldh2 +/+ group and eight mice of the ethanol administered Aldh2 ?/? group died during the 8 days. The weights of mice were decreased by ethanol exposure to 85% and 74% in Aldh2 +/+ and Aldh2 ?/? group, respectively. The survival rates of the ethanol administered Aldh2 +/+ and Aldh2 ?/? group were 40 and 11%. Liver and pancreas disorder was revealed in the ethanol administered Aldh2 +/+ and Aldh2 ?/? group in the results of serum chemical examination, immunohistochemical staining and western blot analysis. Cyp2e1 is more inducible to ethanol toxicity in Aldh2 ?/? mice compared with Aldh2 +/+ mice when ethanol is administered according to the results of quantitative PCR.
  • Yong-Dae Kim, Sang-Yong Eom, Masanori Ogawa, Tsunehiro Oyama, Toyohi Isse, Jong-Won Kang, Yan Wei Hang, Toshihiro Kawamoto, Heon Kim
    JOURNAL OF OCCUPATIONAL HEALTH 49 (5) 363 - 369 1341-9145 2007/09 [Refereed][Not invited]
     
    Excessive alcohol consumption is associated with increased risks of many diseases including cancer. We evaluated oxidative DNA damage in Aldh2 +/+ and Aldh2 -/- mice after they had been subjected to acute ethanol exposure. Olive tail moment, which was measured using a comet assay, was not increased by ethanol treatment in both Aldh2 +/+ and Aldh2 -/- mice. However, after controlling for the effect of ethanol exposure, the Aldh2 genotype was a significant determinant for Olive tail moments. Although the ethanol treatment significantly increased the hepatic 8-OHdG generation in only Aldh2 +/+ mice, the level of 8-OHdG was the highest in Aldh2 -/- ethanol treated mice. The increase in the level of 8-OHdG was associated with hepatic expression of cytochrome P450 2E1 (CYP2E1). The levels of Olive tail moment and the hepatic 8-OHdG in the Aldh2 -/- control group were significantly higher than those of the Aldh2 +/+ control group. The level of CYP2E1 in liver tissue showed a similar pattern to those of the oxidative DNA damage markers. This study shows that acute ethanol consumption increases oxidative DNA damage and that expression of CYP2E1 protein may play a pivotal role in the induction of oxidative DNA damage. The finding that oxidative DNA damage was more intense in Aldh2 -/- mice than in Aldh2 +/+ mice suggests that ALDH2-deficient individuals may be more susceptible than wild-type ALDH2 individuals to ethanol-mediated liver disease, including cancer.
  • Masanori Ogawa, Yoshiaki Nakajima, Yoko Endo
    JOURNAL OF OCCUPATIONAL HEALTH 49 (5) 402 - 404 1341-9145 2007/09 [Refereed][Not invited]
  • Sang-Yong Eom, Yan Wei Zhanga, Masanori Ogawa, Tsunehiro Oyama, Toyohi Isse, Jong-Won Kanga, Lee Chung-Jong, Yong-Dae Kim, Toshihiro Kawamoto
    JOURNAL OF HEALTH SCIENCE 53 (4) 378 - 381 1344-9702 2007/08 [Refereed][Not invited]
     
    Acetaldehyde production during ethanol metabolism has been implicated as an important link between oxidative stress and cell damage, which suggests that oxidative stress caused by ethanol exposure may be more severe in aldehyde dehydrogenase 2 (ALDH2)-deficient individuals than in those with wild-type ALDH2. We evaluated the activities of the major antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), in liver tissue isolated from Aldh2 +/+ and Aldh2 -/- mice that were exposed to ethanol. The activities of CAT and GPx were significantly increased by ethanol treatment in A1dh2 +/+ mice (3.33-fold and 1.65-fold, respectively). The mean activity of SOD in A1dh2 +/+ mice was 1.46-fold that in the A1dh2 +/+ control group, but the difference was not statistically significant. In Aldh2 -/- mice, the activities of SOD and CAT were decreased and that of GPx was slightly increased after ethanol exposure, but the differences were not significant. We postulate that antioxidant enzyme expression after ethanol consumption may differ according to the intracellular level of acetaldehyde or free radicals, which in turn depends on the activity of ALDH2. These results suggest that the greater toxicity of ethanol in Aldh2 -/- mice than in Aldh2 +/+ mice may be due to decreased antioxidant enzyme expression.
  • Ryuichi Kubota, Yoko Endo, Akito Takeuchi, Yoshinori Inoue, Hiroko Ogata, Masanori Ogawa, Tomoo Nakagawa, Nobuhiko Onda, Ginji Endo
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 854 (1-2) 204 - 210 1570-0232 2007/07 [Refereed][Not invited]
     
    An analytical method using a combination of solid-phase extraction (SPE) and gas chromatography with a flame thermionic detector (GC/FTD) was developed for determination of N-methyl-2-pyrrolidone (NMP), N-methylsuccinimide (NISI), and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine. The SPE cartridge of poly(divinylbenzene/hydroxymethacrylate) used was directly loaded with urine sample, followed by elution with methyl isobutyl ketone (MIBK) and subsequent centrifugation, and the supernatant was injected into the capillary GC using a DB 1701. This method allowed efficient separation of NMP, MSI, and 2-HMSI, which were nearly free of interference by other GC peaks arising from urine. Recoveries of NMP, MSI, and 2-HMSI from the SPE cartridge were about 98, 101, and 67%, respectively, with limits of detection of 0.04, 0.02, and 0.06 mg/L, respectively, which met the regulatory requirements. The present method was used for assay in biological monitoring of workers exposed to NMP in their occupational environment. (C) 2007 Elsevier B.V. All rights reserved.
  • Akihisa Hata, Yoko Endo, Yoshiaki Nakajima, Maiko Ikebe, Masanori Ogawa, Noboru Fujitani, Ginji Endo
    JOURNAL OF OCCUPATIONAL HEALTH 49 (3) 217 - 223 1341-9145 2007/05 [Refereed][Not invited]
     
    The toxicity and carcinogenicity of arsenic depend on its species. Individuals living in Japan consume much seafood that contains high levels of organoarsenics. Speciation analysis of urinary arsenic is required to clarify the health risks of arsenic intake. There has been no report of urinary arsenic analysis in Japan using high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). We performed speciation analysis of urinary arsenic for 210 Japanese male subjects without occupational exposure using HPLC-ICP-MS. The median values of urinary arsenics were as follows: sodium arsenite (AsIII), 3.5; sodium arsenate (AsV), 0.1; monomethylarsonic acid (MMA), 3.1; dimethylarsinic acid (DMA), 42.6; arsenobetaine (AsBe), 61.3; arsenocholine, trimethylarsine oxide, and unidentified arsenics (others), 5.2; and total arsenic (total As), 141.3 mu gAs/1. The median creatinine-adjusted values were as follows: AsIII, 3.0; AsV, 0.1; MMA, 2.6; DMA, 35.9; AsBe, 52.1; others 3.5; and total As, 114.9 mu gAs/g creatinine. Our findings indicate that DMA and AsBe levels in Japan are much higher than those found in Italian and American studies. It appears that the high levels of DMA and AsBe observed in Japan may be due in part to seafood intake. ACGIH and DFG set the BEI and BAT values for occupational arsenic exposure as 35 mu gAs/l and 50 mu gAs/l, respectively, using the sum of inorganic arsenic (iAs), MMA, and DMA. In the general Japanese population, the sums of these were above 50 mu gAs/l in 115 (55%) samples. We therefore recommend excluding DMA concentration in monitoring of iAs exposure.
  • Tsunehiro Oyama, Toyohi Isse, Masanori Ogawa, Manabu Muto, Iwao Uchiyama, Toshihiro Kawamoto
    FRONTIERS IN BIOSCIENCE 12 1927 - 1934 1093-9946 2007/01 [Refereed][Not invited]
     
    In this study, we evaluated the inhalation toxicity of acetaldehyde in Aldh2 KO ( Aldh-/-) mice, using pathological method. Male C57BL/6 ( Aldh+/+) mice and Aldh -/- mice were exposed to atmospheres containing acetaldehyde at levels of 0, 125, and 500 ppm for 24 h/day during 14 days. Although the average blood acetaldehyde concentration of Aldh -/- mice was higher than that of Aldh2+/+ mice in the acetaldehyde exposure group, observable effects by the acetaldehyde exposure on the lung and liver were not different between wild type and ALDH2 null mice. In Aldh2-/- mice, the levels of 1) erosion of respiratory epithelium and the subepithelial hemorrhage in nose, 2) hemorrhage in nasal cavity, 3) degeneration of respiratory epithelium in larynx, pharynx and trachea, and 4) degeneration of dorsal skin were higher compared with Aldh2+/+ mice, indicating that Aldh2-/- mice are more acetaldehyde-sensitive than Aldh2+/+ mice. This is the first example for studying pathological effects of Aldh2 deficiency using Aldh-/- mice exposed to a low level of acetaldehyde.
  • Masanori Ogawa, Tsunehiro Oyama, Toyohi Isse, Koichi Saito, Yoshitaka Tomigahara, Yoko Endo, Toshihiro Kawamoto
    TOXICOLOGY LETTERS 168 (2) 148 - 154 0378-4274 2007/01 [Refereed][Not invited]
     
    In order to clarify the effects of ALDH2 polymorphism on the carcinogenicity and organ damage caused by ethanol consumption, labeled ethanol was administered to wild-type (C57BL/6, Aldh2+/+) and Aldh2 knock-out (Aldh2-/-) mice, and DNA adduct levels of organs were compared according to Aldh2 genotype. Aldh2-/- mice, which have the same genetic background as C57BL/6 mice except in the Aldh2 gene, were used as a model of lack of ALDH2 activity in humans. The DNA adduct levels in liver, stomach, and kidney and radioactivity in liver, stomach, kidney, and serum were measured by liquid scintillation counting 6, 12, and 24 It after administration. Though radioactivity levels in all organs decreased over time, there were no significant differences in radioactivity between Aldh2+/+ and Aldh2-/- mice. On the other hand, the DNA radioactivity in each organ tested differed significantly between Aldh2+/+ and Aldh2-/- mice 24h after administration. These findings show that ethanol consumption affects DNA in Aldh2-/- mice much more strongly than in Aldh2+/+ mice. According to the IARC document, ethanol consumption is carcinogenic to humans (Group 1). Moreover, several studies have shown that ALDH2-deficient humans who habitually consume ethanol have higher rates of cancer than humans with ALDH2. Our results support these findings of epidemiological studies. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
  • Fujita H, Sawada Y, Ogawa M, Endo Y
    Sangyo eiseigaku zasshi = Journal of occupational health 1 49 (1) 1 - 8 1341-0725 2007/01 [Refereed][Not invited]
  • Rie Narai, Tsunehiro Oyama, Masanori Ogawa, Tetsunosuke Yamaguchi, Tsuyoshi Kinaga, Tomoe Murakami, Toyohi Isse, Shin-ichi Ozaki, Yasunori Yashima, Ken Okabayashi, Hideo Ochiai, Keiichiro Yarita, Akihiro Fujino, Toshihiro Kawamoto
    JOURNAL OF OCCUPATIONAL HEALTH 49 (1) 9 - 16 1341-9145 2007/01 [Refereed][Not invited]
     
    Around three million Japanese are persistently infected with HBV or HCV. Though most of them work in various industries, little is known about the actual conditions in their workplaces. To clarify the workplace conditions of workers with hepatitis, three kinds of questionnaire surveys, answered by occupational health physicians and workers with hepatitis, were carried out. The rates of workers recognized as workers with hepatitis B or C by occupational health physicians were 0.82% and 0.48% of 130,092 workers, respectively. About 30% of workers with hepatitis were engaged in "hazardous work". The percentage of workers engaged in various types of hazardous work among workers with hepatitis was nearly the same as that among all Japanese workers. About 30% of occupational health physicians witnessed exacerbation of hepatitis in the workers at their workplaces, and 22% of workers with hepatitis experienced exacerbation of hepatitis. The rate of workers with hepatitis who had experienced exacerbation was not significantly different between workers with and without hazardous work. Workers with hepatitis have strong, concerns about the relationship between work and exacerbation. As causes of exacerbation, occupational health physicians cited "unknown", "drinking" and "quit treatment" while workers with hepatitis answered "work-related causes", besides "unknown" and "drinking."
  • Tomoe Murakami, Tsunehiro Oyama, Toyohi Isse, Masanori Ogawa, Takuya Sugie, Toshihiro Kawamoto
    Environmental Health and Preventive Medicine 12 (2) 56 - 65 1342-078X 2007 [Refereed][Not invited]
     
    In this study, we aim to compare the criteria for sensitizers among national organizations in various countries and international organizations, and to specify whether each Pollutant Release and Transfer Register (PRTR)-designated chemical substance is a sensitizer by each organization. The definition of sensitizing chemicals and the designation of respective sensitizers according to the PRTR law, Japan Society for Occupational Health (JSOH), American Conference of Governmental Industrial Hygienists (ACGIH), European Union (EU), and Deutsche Forschungsgemeinshaft (DFG) were studied. Of the 435 PRTR-designated chemical substances, 15 are listed as sensitizers according to the PRTR law, 16 as sensitizers of the airway and 21 as sensitizers of the skin by JSOH, 12 as sensitizers (no discrimination) by ACGIH, 19 (airway) and 85 (skin) by EU, and 15 (airway) and 43 (skin) by DFG. Only 9 substances were designated as sensitizers by all these organizations. The variation in the designation of sensitizers is accounted for by the differences in the classification criteria and grouping of chemical substances. JSOH limits the definition of sensitizers to substances that induce allergic reactions in humans and uses only human data. Other organizations utilize not only human evidence but also appropriate animal tests. In addition, EU designates an isocyanate as a sensitizer except those for which there is evidence showing that they do not cause respiratory sensitivity. The worldwide enforcement of the globally harmonized system (GHS) of classification and labeling of chemicals could promote not only the consistent designation of sensitizers among national and international organizations, but also the development of testing guidelines and classification criteria for mixtures.
  • Hiroshi Fujita, Masanori Ogawa, Yoko Endo
    JOURNAL OF OCCUPATIONAL HEALTH 48 (6) 413 - 416 1341-9145 2006/11 [Refereed][Not invited]
  • Masanori Ogawa, Tsunehiro Oyama, Toyohi Isse, Tetsunosuke Yamaguchi, Tomoe Murakami, Yoko Endo, Toshihiro Kawamoto
    JOURNAL OF OCCUPATIONAL HEALTH 48 (5) 314 - 328 1341-9145 2006/09 [Refereed][Not invited]
     
    Health-Many new biomarkers are being studied, in addition to classical biomarkers, such as chemical substances and their metabolites in blood and urine and modified enzymes. Among these new biomarkers, hemoglobin adducts are thought to be especially useful for the estimation of chemical exposures. We review here the use of biomarkers for monitoring exposures to nine substances, mainly focusing on PRTR class I designated chemical substances, styrene, phenyloxirane (styrene oxide), 4,4'-methylendiphenyl diisocyanate (MDI), 4,4'-methylendianiline (MDA), 1,3-butadiene, ethylene oxide, propylene oxide, acrylamicle and acrylonitrile. Hemoglobin adduct levels were elevated after exposures to styrene, MDI, MDA, 1, 3-butadiene ethylene oxide, acrylamide and acrylonitrile. Moreover: hemoglobin adducts of butadiene, ethylene oxide, acrylamide and acrylonitrile have several useful advantages. For example, the hemoglobin adduct of 1,3-butadiene is an even more useful biomarker of exposure than urinary metabolites, and in the case of ethylene oxide, even though the concentration of ethylene oxide-Hb in the blood of workers did not exceed the value of the German exposure equivalent, a significant difference in it was found between workers and a control group. Also hemoglobin adducts of acrylamide and acrylonitrile can reflect their exposures because there are no urinary metabolites of acrylamide and acrylonitrile that are useful for exposure assessment. In addition to these advantages, hemoglobin adducts are superior to DNA adducts with respect to the availability of large amounts, availability of methods for chemical identification, and well-defined life spans due to the absence of repair. Hemoglobin adducts can be effective biomarkers for assessing exposure to and the effects of chemicals.
  • Tsunehiro Oyama, Toyohi Isse, Tomoe Murakami, Rie Suzuki-Narai, Masanori Ogawa, Tetsunosuke Yamaguchi, Tsuyoshi Kinaga, Yasunori Yashima, Shinichi Ozaki, Yong-Dae Kim, Heon Kim, Toshihiro Kawamoto
    Environmental Health and Preventive Medicine 11 (3) 97 - 101 1342-078X 2006/05 [Refereed][Not invited]
     
    The number of fatalities in Japan attributable to lung cancer exceeded 50000 in 2001. It is socially desirable that various markers, which can be utilized for the prevention of lung cancer, be established. We believe that smoking or exposure to carcinogens in air induces mutations in bronchial and alveolar epithelia, leading to the development of lung cancer. It would be useful to have markers of individual differences in susceptibility to chemical carcinogen-induced lung cancer 1) to identify genetic polymorphisms of enzymes metabolizing chemical carcinogens and 2) to investigate the expression of enzymes metabolizing chemical carcinogens. In this paper, we review CYP expression in the bronchial epithelium. CYP1, CYP2 and CYP3 are expressed in the bronchial epithelium. We also show the relationship between the genetic polymorphisms of cytochrome P450 (CYP) and a person's susceptibility to chemical carcinogen-induced lung cancer. We demonstrate the relationship between cigarette consumption and the CYP expression profile in the bronchial epithelium. To maintain and promote public health, we must apply evidence, such as CYP polymorphisms and CYP profiles to disease prevention and also to aggressively advance evidence-based prevention (EBP) of lung cancer.
  • M Ogawa, T Isse, T Oyama, N Kunugita, T Yamaguchi, T Kinaga, R Narai, A Matsumoto, YD Kim, H Kim, Uchiyama, I, T Kawamoto
    INDUSTRIAL HEALTH 44 (1) 179 - 183 0019-8366 2006/01 [Refereed][Not invited]
     
    To clarify the carcinogenicity of acetaldehyde when associated with ALDH (aldehyde dehydrogenase) 2 polymorphism, Aldh2 knock-out (Aldh2-/-) mice and their wild type (Aldh2+/+) mice were exposed to two different concentrations of acetaldehyde (125ppm and 500ppm) for two weeks. Aldh2-/- mice, which have the same genetic background as C57BL/6J (wild mice) except for the Aldh2 gene, were used as models of humans who lack ALDH2 activity. Urinary 8hydroxydeoxyguanosine (8-OHdG) and plasma malondialdehyde (MDA) levels were measured as indicators of oxidative DNA damage and lipid peroxidation, respectively. At 125 ppm acetaldehyde exposure for 12 d, urinary 8-OHdG levels in Aldh2+/+ mice did not increase. However, urinary 8-OHdG levels in Aldh2-/- mice were slightly increased by the end of the exposure. On the other hand, plasma MDA levels did not increase in either Aldh2-/- or Aldh2+/+ mice. At 500 ppm, urinary 8-OHdG levels in both Aldh2-/- and Aldh2+/+ mice significantly increased after 6 and 12 d, but there was no genetic difference. On the other hand, plasma MDA levels in Aldh2+/+ and Aldh2-/-mice did not increase at either 125 ppm or 500 ppm after two weeks of exposure. In conclusion, it is suspected that DNA was damaged by acetaldehyde inhalation, and that susceptibility to acetaldehyde varies according to Aldh2 genotype.
  • Toyohi Isse, Tsunchiro Oyama, Koji Matsuno, Masanori Ogawa, Ric Narai-Suzuki, Tetsunosuke Yamaguchi, Tomoe Murakami, Tsuyoshi Kinaga, Iwao Uchiyama, Toshihiro Kawamoto
    Journal of Toxicological Sciences 30 (4) 329 - 337 0388-1350 2005/12 [Refereed][Not invited]
     
    Aldehyde dehydrogenase 2 (ALDH2) is an important enzyme that oxidizes acetaldehyde. Approximately 45% of Chinese and Japanese individuals have the inactive ALDH2 genotypes (ALDH2*2/ *2 and ALDH2*1/*2) acute inhalation toxicity of acetaldehyde has not been evaluated in these populations. We compared the toxicity between wild-type (Aldh2+/+) and Aldh2-inactive transgenic (Aldh2-/-) mice by using the paired acute inhalation test modified from the acute toxic class method (OECD TG433). Blood acetaldehyde level was measured 4 hr after the inhalation. A pair of Aldh2+/+ and Aldh2-/- mice was put into a chamber and was exposed to 5000 ppm of acetaldehyde. At the start of the inhalation, the mice exhibited hypoactivity and closing of the eyes. Subsequently, symptoms such as crouching, bradypnea, and piloerection were observed. Flushing was observed only in the Aldh2+/+ mice. Symptoms such as tears, straggling gait, prone position, pale skin, abnormal deep respiration, dyspnea, and one case of death were observed only in the Aldh2-/- mice. The symptoms did not change 1 hr after inhalation in the Aldh2+/+ mice. In contrast, in the Aldh2-/- mice, the symptoms became more severe until the end of the inhalation. The blood acetaldehyde level in the Aldh2-/- mice was approximately twice that in the Aldh2+/+ mice 4 hr after inhalation. The Aldh2-/- mice evidently showed more severe toxicity as compared with the Aldh2+/+ mice due to acute inhalation of acetaldehyde at a concentration of 5000 ppm. Acetaldehyde toxicity in Aldh2+/+ and Aldh2-/- mice was estimated and classified one class different. Based on this study, acetaldehyde inhalations were inferred to pose a higher risk to ALDH2-inactive human individuals.
  • T Oyama, N Kagawa, N Kunugita, K Kitagawa, M Ogawa, T Yamaguchi, R Suzuki, T Kinaga, Y Yashima, S Ozaki, T Isse, YD Kim, H Kim, T Kawamoto
    FRONTIERS IN BIOSCIENCE-LANDMARK 9 1967 - 1976 1093-9946 2004/05 [Refereed][Not invited]
     
    CYPs (cytochrome P450s) catalyze the conversion of numerous numbers of xenobiotics including carcinogens and drugs. CYPs can be involved in metabolic pathways of activation of procarcinogens and/or inactivation of carcinogens during the tumorigenic processes. Recently, increasing number of cancer tissues as well as normal tissues have been found to express a variety of CYPs. The local expression of CYPs in tumors appears to be very important for the management of cancers since CYPs expressed in tumors may be involved in activation and/or inactivation of anticancer drugs. The expression of CYPs in tumors may also convert endogenous substrates to metabolites that facilitate cancer development. In this review, we summarize the association of CYP expression in cancer tissues with carcinogenesis and cancer treatment.

Books etc

  • Coffee in Health and Disease
    Ogawa Masanori (ContributorCoffee and Hippuric Acid)
    Elsevier 2014
  • レアメタル便覧
    小川 真規 (Joint workコバルト、ニッケル)
    丸善 2010
  • 「病気予防」百科
    小川 真規 (Joint workシックハウス症候群)
    日本医療企画 2007
  • 日本医事新報
    小川 真規 (Joint work有機溶剤の測定と体内動態)
    日本医事新報社 2006/09

MISC

Research Grants & Projects

  • 交流分析を用いた、新人看護師の就労支援の在り方の検討
    日本学術振興会:
    Date (from‐to) : 2019/04 -2023/03 
    Author : 小川 真規
  • 海外派遣労働者に対する適切な感染症対策の検討-労働者・産業医の視点から
    日本学術振興会:
    Date (from‐to) : 2014/04 -2017/03 
    Author : 小川 真規
  • 心理学と情報工学を利用した食品リスク情報コミュニケーションツールとその評価手法の開発
    厚生労働省:厚生科研費
    Date (from‐to) : 2012/04 -2014/03 
    Author : 和田有史
  • アルミニウム作業者における尿中アルミニウムを用いた生体影響評価
    日本学術振興会:
    Date (from‐to) : 2011/04 -2014/03 
    Author : 小川 真規

Social Contribution

  • 自治医科大学産業医研修会
    Role : Lecturer
  • 埼玉県立大学 非常勤講師
    Role : Lecturer


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