Researchers Database

hirao atsushi

    BasicSciencerelatedtoNursing Associate Professor
Last Updated :2021/10/17

Researcher Information

J-Global ID

Research Interests

  • 尾腺   社会行動   嗅上皮   GFPトランスジェニックブタ   

Research Areas

  • Life sciences / Anatomy
  • Life sciences / Veterinary medicine
  • Life sciences / Veterinary medicine
  • Life sciences / Veterinary medicine

Academic & Professional Experience

  • 2012/04 - Today  Jichi Medical University看護学部講師

Published Papers

  • Yuumi Ishizuka, Kazuhiro Nakayama, Ayumi Ogawa, Saho Makishima, Supichaya Boonvisut, Atsushi Hirao, Yusaku Iwasaki, Toshihiko Yada, Yoshiko Yanagisawa, Hiroshi Miyashita, Masafumi Takahashi, Sadahiko Iwamoto
    JOURNAL OF MOLECULAR ENDOCRINOLOGY 52 (2) 145 - 158 0952-5041 2014/04 [Refereed][Not invited]
    Mammalian tribbles homolog 1 (TRIB1) regulates hepatic lipogenesis and is genetically associated with plasma triglyceride (TG) levels and cholesterol, but the molecular mechanisms remain obscure. We explored these mechanisms in mouse livers transfected with a TRIB1 overexpression, a shRNA template or a control (LacZ) adenovirus vector. The overexpression of TRIB1 reduced, whereas induction of the shRNA template increased, plasma glucose, TG, and cholesterol and simultaneously hepatic TG and glycogen levels. The involvement of TRIB1 in hepatic lipid accumulation was supported by the findings of a human SNP association study. A TRIB1 SNP, rs6982502, was identified in an enhancer sequence, modulated enhancer activity in reporter gene assays, and was significantly (P = 9.39 x 10(-7)) associated with ultrasonographically diagnosed nonalcoholic fatty liver disease in a population of 5570 individuals. Transcriptome analyses of mouse livers revealed significant modulation of the gene sets involved in glycogenolysis and lipogenesis. Enforced TRIB1 expression abolished CCAAT/enhancer binding protein A (CEBPA), CEBPB, and MLXIPL proteins, whereas knockdown increased the protein level. Levels of TRIB1 expression simultaneously affected MKK4 (MAP2K4), MEK1 (MAP2K1), and ERK1/2 (MAPK1/3) protein levels and the phosphorylation of JNK, but not of ERK1/2. Pull-down and mammalian two-hybrid analyses revealed novel molecular interaction between TRIB1 and a hepatic lipogenic master regulator, MLXIPL. Co-expression of TRIB1 and CEBPA or MLXIPL reduced their protein levels and proteasome inhibitors attenuated the reduction. These data suggested that the modulation of TRIB1 expression affects hepatic lipogenesis and glycogenesis through multiple molecular interactions.
  • Natalie F. Shanks, Jeffrey N. Savas, Tomohiko Maruo, Ondrej Cais, Atsushi Hirao, Souichi Oe, Anirvan Ghosh, Yasuko Noda, Ingo H. Greger, John R. Yates, Terunaga Nakagawa
    CELL REPORTS 1 (6) 590 - 598 2211-1247 2012/06 [Refereed][Not invited]
    AMPA receptor (AMPA-R) complexes consist of channel-forming subunits, GluA1-4, and auxiliary proteins, including TARPs, CNIHs, synDIG1, and CKAMP44, which can modulate AMPA-R function in specific ways. The combinatorial effects of four GluA subunits binding to various auxiliary subunits amplify the functional diversity of AMPA-Rs. The significance and magnitude of molecular diversity, however, remain elusive. To gain insight into the molecular complexity of AMPA and kainate receptors, we compared the proteins that copurify with each receptor type in the rat brain. This interactome study identified the majority of known interacting proteins and, more importantly, provides candidates for additional studies. We validate the claudin homolog GSG1L as a newly identified binding protein and unique modulator of AMPA-R gating, as determined by detailed molecular, cellular, electrophysiological, and biochemical experiments. GSG1L extends the functional variety of AMPA-R complexes, and further investigation of other candidates may reveal additional complexity of ionotropic glutamate receptor function.
  • Tatsuo Kawarasaki, Kazuhiko Uchiyama, Atsushi Hirao, Sadahiro Azuma, Masayoshi Otake, Masatoshi Shibata, Seiko Tsuchiya, Shin Enosawa, Koichi Takeuchi, Kenjiro Konno, Yoji Hakamata, Hiroyuki Yoshino, Takuya Wakai, Shigeo Ookawara, Hozumi Tanaka, Eiji Kobayashi, Takashi Murakami
    JOURNAL OF BIOMEDICAL OPTICS 14 (5) 054017  1083-3668 2009/09 [Refereed][Not invited]
    Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3241985]
  • Yuko Maejima, Masato Aoyama, Shigeo Ookawara, Atsushi Hirao, Shoei Sugita
    VETERINARY JOURNAL 181 (2) 193 - 199 1090-0233 2009/08 [Refereed][Not invited]
    Previously it has been shown that androgen suppresses transportation-induced increases in plasma adrenocorticotropic hormone (ACTH), possibly by suppressing the secretion of corticotrophin releasing hormone (CRH) or arginine vasopressin (AVP) from the hypothalamus, or secretion of ACTH from the pituitary gland. The aim of the present study was to examine androgen target sites in the caprine diencephalon and pituitary gland using immunohistochemical methods. The androgen receptor (AR) was expressed strongly in the bed nucleus of the stria terminalis, the medial preoptic area, the arcuate nucleus, the ventromedial hypothalamic nucleus and the suprachiasmatic nucleus in the diencephalon. Between 8%, and 11% of CRH and AVP neurons in the paraventricular hypothalamic nucleus (PVN) expressed AR. In the pituitary gland, 7.1%, of corticotrophs expressed AR. The results are consistent with the proposal that androgen acts directly and indirectly on CRH and/or AVP neurons in the PVN. The possibility of a direct action of androgen oil the corticotrophs in the pituitary gland was also considered. (c) 2008 Elsevier Ltd. All rights reserved.
  • Atsushi Hirao, Masato Aoyama, Shoei Sugita
    BEHAVIOURAL PROCESSES 80 (2) 115 - 120 0376-6357 2009/02 [Refereed][Not invited]
    Recent studies have indicated that avian social behavior is influenced by olfactory cues. During the reproductive season a change in the chemical composition of uropygial gland secretion has been reported in some species and the hypothesis that olfactory signals may be produced by this gland has been proposed. To examine this hypothesis we performed two behavioral experiments to determine whether a female's uropygial gland produce chemical signals that Stimulate mating behaviors in domestic chickens. In Experiment 1 the role of the female's uropygial gland in male mating behavior was examined by removing and examining the female's uropygial gland. The frequency of mounts and copulations of intact male birds with sham-operated female birds was significantly higher than with uropygial glandectomized female birds. With respect to the number of waltzing that is one of the courtship displays intact males showed no significant difference between sham-operated female birds and uropygial glandectomized female birds. In Experiment 2 the relationship between male olfaction and the female's uropygial gland was investigated using olfactory bulbectomized male birds. The number of mounts and copulations of sham-operated male birds with sham-operated female bird was significantly higher than with uropygial glandectomized female birds. In contrast olfactory bulbectomized male birds showed no significant differences in the number of mounts and copulations between sham-operated female birds and uropygial glandectomized female birds. These results indicate that intact and sham-operated male birds prefer to mate with female birds with the uropygial gland. The number of courtship waltzing of sham-operated male birds showed no significant difference. However olfactory bulbectomized male birds significantly courted to uropygial glandectomized female birds. Summarizing our results show that while anosmic males did not have any preference both intact and sham-operated male birds chose to mate with female birds having an intact uropygial gland suggesting that mate preference involves in male olfaction and that the female's uropygial gland acts as a source of social odor Cues in domestic chickens. (C) 2008 Elsevier B.V. All rights reserved.
  • Hirao Atsushi, Kawarasaki Tatsuo, Konno Kenjiro, Takeuchi Koichi, Otake Masayoshi, Ookawara Shigeo, Kobayashi Eiji
    The Japanese journal of taste and smell research 日本味と匂学会 15 (3) 565 - 568 1340-4806 2008/12 [Not refereed][Not invited]
  • M Suzuki, Y Watanabe, Y Oyama, A Mizuno, E Kusano, A Hirao, S Ookawara
    NEUROSCIENCE LETTERS 353 (3) 189 - 192 0304-3940 2003/12 [Refereed][Not invited]
    A transient receptor potential (TRP) family, TRPV4, is a calcium-permeable swell-activated channel, playing a role in cutaneous mechanosensation. To elucidate the localization in the mechanosensitive endings, we found with immunohistochemistry in mice that TRPV4 was expressed both by small (low threshold) and large (high threshold) dorsal root ganglia neurons. In addition to free nerve endings, TRPV4 was specifically located at cutaneous mechanosensory terminals co-localized with neurofilament 200, including Meissner, Merkel, penicillate and intraepidermal terminals but not including hair follicle palisades. The distribution suggests that the sensation of pressure by mechanosensitive TRPV4 channel is transmitted through A- as well as C-fiber. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
  • M Suzuki, A Hirao, A Mizuno
    JOURNAL OF BIOLOGICAL CHEMISTRY 278 (51) 51448 - 51453 0021-9258 2003/12 [Refereed][Not invited]
    The molecular mechanism of the transmission of changes in the shape of the cell surface to ion channels remains obscure. Ca2+ influx induced by cell deformity is inhibited by actin-freezing reagents, suggesting that the actin microfilament couples with an ion channel. Transient receptor potential vanilloid 4 (TRPV4) is a candidate in the calcium-permeable, swelling-activated mechanosensitive channel in heterogeneously expressed cells. To investigate the mechanosensitive molecular complex, we found that microtubule-associated protein 7 (MAP7) is the mouse TRPV4 C-terminal binding protein. MAP7 was coimmunoprecipitated with TRPV4. The results of a pull-down assay demonstrated that the alignment of amino acids 798-809 of TRPV4 was important in this interaction. TRPV4 and MAP7 colocalized in the lung and kidney. The coexpression of these two molecules resulted in the redistribution of TRPV4 toward the membrane and increased its functional expression. The alignment of amino acids 798-809 of TRPV4 was also important in the functional expression. The activated current was abolished by actin-freezing but not by microtubule-freezing reagents. We therefore believe that MAP7 may enhance the membrane expression of TRPV4 and possibly link cytoskeletal microfilaments.
  • T Morinaga, M Nakakoshi, A Hirao, M Imai, K Ishibashi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 294 (3) 630 - 634 0006-291X 2002/06 [Refereed][Not invited]
    AQP10 is the newest member of aquaporins in mammals and expressed selectively in the duodenum and the jejunum in human functioning as aquaglyceroporin. Here we report the cloning of the mouse AQP10 gene. The gene is composed of six exons and spans 5.2 kb. The arrangement of the exons is well conserved between mouse and human. However, the initiator methionine is lost because of the mutation at the translation-initiation site. An insertion of four thymine residues in exon 2 and a deletion of a cytosine residue in exon 5 shift the reading frame. Moreover, aberrant exon/intron junction sequences of introns 2, 3, and 4 also shift the reading frame between exons. Genomic Southern blot revealed the mouse AQP10 gene as a single copy gene. The results indicate that the mouse AQP10 gene is a pseudogene. Furthermore, the mouse AQP10 transcript was not detected in the jejunum where the human AQP10 is strongly expressed. (C) 2002 Elsevier Science (USA). All rights reserved.
  • HIRAO Atsushi, SUGITA Shoei, SUGAHARA Kunio
    Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 71 (10) 483 - 490 1346-907X 2000 [Not refereed][Not invited]
    In order to clarify olfactory system of birds, the efferent and afferent connections of chicken olfactory bulb were investigated using with biocytin and horseradish peroxidase (HRP) methods. From the results of biocytin method, the efferent pathways of chicken olfactory bulb were classified into route 1-3.Route 1 projected to the ipsilateral hyperstriatum accessorium (HA), and hippocampus (Hp). Route 2 had the projections to ipsilateral hyperstriatum intercalatum supremum, hyperstriatum ventrale (HV), neostriatum (N), and nucleus basalis (Bas). Route 3 projected to the bilateral lobus parolfactorius, tuberculum olfactorium tractus fronto-archistriaticus, paleostriatum augmentatum, archistriatum anterior, cortex piriformis, area temporo-parieto-occipitalis, nucleus taeniae, contralateral HA, Hp, HV, N, and Bas. From the results of HRP method, the chicken olfactory bulb received the projections from nucleus septalis medialis, nucleus septalis lateralis, and Hp. In addition, it was appeared that the chicken olfactory bulb has the reciprocal connection with Hp.
  • HIRAO Atsushi, SUGITA Shoei, FUJIWARA Katsuhiko
    Nihon Chikusan Gakkaiho 公益社団法人 日本畜産学会 65 (9) 842 - 849 1346-907X 1994 [Not refereed][Not invited]
    The efferent and afferent connections of the bulbus olfactorium (BO) in chickens (Gallus gallus domesticus) were studied using the biocytin and horseradish peroxidase (HRP) methods. Twelve and seven fowls were used for biocytin and HRP studies, respectively. After biocytin was injected into the unilateral BO, many labeled fibers and terminal grains were seen in the bilateral tuberclum olfactorium. Labeled fibers and terminal grains were also found in the lobes parolfactorius, nucleus basalis, neostriatum (N), hyperstriatum accessorium (HA), paleostriatum augumentum (PA), archistriatum pars ventralis (AV), nucleus taeniae, cortex piriformis (CPi) and nucleus septomesencephalici (nSMT), bilaterally. After HRP was injected into the unilateral BO, retrogradely labeled neurons were found bilaterally in the HA as well as the nucleus septalis lateralis and medialis. These results show that the projections of a chicken BO in regard to CPi, Av, and TO are similar to projections of a mammalian's olfactory bulb. A projection from BO to nSMT is similar to that of a reptlian. In addition, projections of BO in regard to the PA, and projections f S to BO in the chicken have not been reported for other vertebrates.


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